In vivo gamma imaging of the secondary tumors of transplanted human fetal striatum neural stem cells-derived primary tumor cells.

Abstract:

:This study describes gamma-imaging of the secondary tumors from the transplanted human fetal striatum neural stem cells-derived primary tumor cells in nude mice. The subcutaneous primary tumors were detected to express integrin alphavbeta3, and the corresponding cells were isolated and enriched in vitro, then transplanted to the nude mice. The technetium-99m-labeled Arg-Gly-Asp peptide, with high affinity to integrin alphavbeta3, was prepared for biodistribution and gamma-imaging. The secondary tumors were readily visualized at 1-h postinjection, and the tumor uptake of radiotracer was similar to that of positive control animals transplanted with U87MG human glioma cells. The tumor specificity of radiotracer was demonstrated by blocking experiment. We concluded that gamma-imaging is a promising approach in imaging the tumorigenesis of transplanted stem cells in vivo.

journal_name

Neuroreport

journal_title

Neuroreport

authors

He Q,Liu Z,Jia B,Li X,Shi J,Zhang J,Lan F,Yang Z,Liu Y,Shen L,Wang F

doi

10.1097/WNR.0b013e328303c020

subject

Has Abstract

pub_date

2008-07-02 00:00:00

pages

1009-14

issue

10

eissn

0959-4965

issn

1473-558X

pii

00001756-200807020-00003

journal_volume

19

pub_type

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