Flow cytometric DNA analysis of hepatocellular carcinoma.

Abstract:

:The prognostic value of nuclear DNA content was studied retrospectively using flow cytometry in 203 cases of resected hepatocellular carcinoma. The occurrence of DNA aneuploidy, which was detected in 50% of patients, correlated significantly with tumor size and the presence of vascular invasion or intrahepatic metastasis. Overall, patients with DNA aneuploid tumors had a significantly worse prognosis than those with DNA diploid tumors (P less than 0.001) and, also in subdivided groups by tumor size (P less than 0.01). Among DNA aneuploid patients, the survival times were significantly shorter for patients with a low DNA index (less than 1.5) than for those with a high DNA index (greater than or equal to 1.5) (P less than 0.05). In a Cox multivariate analysis, nuclear DNA content provided significant prognostic value (P = 0.008), as did vascular invasion (P = 0.001) and intrahepatic metastasis (P = 0.005). These results indicated that nuclear DNA content has an important prognostic value in hepatocellular carcinoma.

journal_name

Cancer

journal_title

Cancer

authors

Fujimoto J,Okamoto E,Yamanaka N,Toyosaka A,Mitsunobu M

doi

10.1002/1097-0142(19910215)67:4<939::aid-cncr28206

subject

Has Abstract

pub_date

1991-02-15 00:00:00

pages

939-44

issue

4

eissn

0008-543X

issn

1097-0142

journal_volume

67

pub_type

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