Abstract:
:For many years the precise genetic etiology of the majority of Wilms' tumors has remained unexplained. Recently, the WTX gene, mapped to chromosome Xq11.1, has been reported to be lost or mutated in approximately one-third of Wilms' tumors. Moreover, in female cases, the somatically inactivated alleles were found to invariantly derive from the active chromosome X. Consequently, WTX has been proposed as a 'one-hit' tumor suppressor gene. To provide further insights on the contribution of WTX to the development of the disease, we have examined 102 Wilms' tumors, obtained from 43 male and 57 female patients. Quantitative PCR analyses detected WTX deletions in 5 of 45 (11%) tumors from males, whereas loss of heterozygosity at WTX-linked microsatellites was observed in 9 tumors from 50 informative females (19%). However, in the latter group, using a combination of HUMARA assay and bisulfite-modified DNA sequencing, we found that the deletion affected the active chromosome X only in two cases (4%). Sequence analyses detected an inactivating somatic mutation of WTX in a single tumor, in which a strongly reduced expression of the mutant allele respect to the wild-type allele was observed, a finding not consistent with its localization on the active chromosome X. Overall, a functional somatic nullizygosity of the WTX gene was ascertained only in seven of the Wilms' tumors included in the study (approximately 7%). Our findings indicate that previously reported estimates on the proportion of Wilms' tumors due to WTX alterations should be reconsidered.
journal_name
Oncogenejournal_title
Oncogeneauthors
Perotti D,Gamba B,Sardella M,Spreafico F,Terenziani M,Collini P,Pession A,Nantron M,Fossati-Bellani F,Radice Pdoi
10.1038/onc.2008.93subject
Has Abstractpub_date
2008-07-31 00:00:00pages
4625-32issue
33eissn
0950-9232issn
1476-5594pii
onc200893journal_volume
27pub_type
杂志文章相关文献
ONCOGENE文献大全abstract::This issue attempts to give a 'state of the art' overview of the AP-1 transcription factor family, a fundamental class of transcriptional regulators. The AP-1 family consists of several bZIP (basic region leucine zipper) domain proteins, the Jun, the Fos, and the ATF subfamilies, which all have to dimerize before they...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1204416
更新日期:2001-04-30 00:00:00
abstract::The Src family kinases c-Src, and its downstream effectors, the Rho family of small GTPases RhoA and Jun N-terminal kinase (JNK) have a significant role in tumorigenesis. In this report, using the Drosophila wing disc epithelium as a model system, we demonstrate that the actin-Capping Protein (CP) αβ heterodimer, whic...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2013.155
更新日期:2014-04-17 00:00:00
abstract::Constitutive expression of the activated Rap1A protein inhibits differentiation of myogenic C2 cells whereas the inactivated Rap1A protein favours cell differentiation and induces late endocytic compartments clustering. Although the role of Rap1A in MAPK activation has been analysed in various cell types, the signalli...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1203984
更新日期:2000-12-07 00:00:00
abstract::Targeted therapeutics are potential therapeutic agents because of their selectivity and efficacy against tumors resistant to conventional therapy. The goal of this study was to determine the comparative activity of monovalent, engineered anti-Her2/neu immunotoxins fused to recombinant gelonin (rGel) to the activity of...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2012.612
更新日期:2014-01-23 00:00:00
abstract::We have previously reported that platelet-derived growth factor (PDGF) induced tyrosine phosphorylation of GTPase-activating protein (GAP) in intact quiescent fibroblasts under conditions in which insulin and basic fibroblast growth factor (bFGF) were ineffective (Molloy et al., 1988). In the present study, we have pr...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract::NF-kappaB is known to exert a cytoprotective action against TNF-alpha-induced apoptosis. To study the role of NF-kappaB in various TNF-alpha-treated epithelial cell lines, we generated stable transfectants overexpressing a mutated unresponsive form of the IkappaBalpha inhibitor (MT cells). As NF-kappaB prevented TNF-a...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205489
更新日期:2002-05-30 00:00:00
abstract::To investigate the mechanisms of colorectal carcinogenesis, we searched for genes regulated by adenomatous polyposis coli gene product (APC) and identified a novel gene, termed HELAD1 (helicase, APC down-regulated 1). A recombinant polypeptide representing the ATPases associated with cellular activities (AAA) domain o...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205751
更新日期:2002-09-12 00:00:00
abstract::The past three decades have seen an unremitting quest to identify and understand gastrointestinal stem cells, their plasticity in differentiating across cell types, as well as their role in normal, regenerative, and cancer cells. A fascinating hallmark of stem cells is their ability to undergo assymetric cell division...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/sj.onc.1208924
更新日期:2005-08-29 00:00:00
abstract::Scribble complex proteins maintain apicobasal polarity, regulate cell fate determination and function as tumour suppressors in epithelial tissue. Despite evidence that the function of Scribble is maintained in the lymphocyte lineage, we still understand little about its role as a tumour suppressor in haematological ma...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2015.167
更新日期:2016-03-03 00:00:00
abstract::We tested the cytotoxic action of 8-hydroxyguanine (8ohG) by observing the viability of several leukemic cell lines (KG-1, U937, Jurkat and K 562) in the presence of 8-hydroxydeoxyguanosine (8ohdG), a nucleoside of 8ohG. It was found that 8ohdG showed cytotoxic action only to KG-1 and that only KG-1 showed a homozygou...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1203787
更新日期:2000-09-14 00:00:00
abstract::Most cases of breast cancer (BrCa) mortality are due to vascular metastasis. BrCa cells must intravasate through endothelial cells (ECs) to enter a blood vessel in the primary tumor and then adhere to ECs and extravasate at the metastatic site. In this study we demonstrate that inhibition of hypoxia-inducible factor (...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2011.365
更新日期:2012-04-05 00:00:00
abstract::Maspin has been demonstrated to be a suppressor of invasion and cell motility in vitro, whereas in vivo analyses have reported that increased expression of maspin is associated with malignant behavior. The present study examined maspin expression in normal lung and non-small-cell lung cancers. Only proximal airway cel...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1207557
更新日期:2004-05-20 00:00:00
abstract::We report here the nucleic acid sequence and deduced amino acid sequence of a cDNA for TIS7, a gene induced by mitogens in 3T3 cells and by nerve growth factor in PC12 pheochromocytoma cells. A fragment of the TIS7 gene has previously been cloned from murine fibroblast cells infected with Newcastle Disease Virus. The ...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1989-10-01 00:00:00
abstract::Evasion of apoptosis in pediatric acute lymphoblastic leukemia (ALL) is linked to aberrant expression of inhibitor of apoptosis (IAP) proteins and dysregulated redox homeostasis, rendering leukemic cells vulnerable to redox-targeting therapies. Here we discover that inhibition of antioxidant defenses via glutathione (...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2014.338
更新日期:2015-07-30 00:00:00
abstract::Ectopic epigenetic mechanisms play important roles in facilitating tumorigenesis. Here, we first demonstrated that ANKDD1A is a functional tumor suppressor gene, especially in the hypoxia microenvironment. ANKDD1A directly interacts with FIH1 and inhibits the transcriptional activity of HIF1α by upregulating FIH1. In ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-018-0423-9
更新日期:2019-01-01 00:00:00
abstract::Mdm2 is the major negative regulator of p53 tumor-suppressor activity. This oncoprotein is overexpressed in many human tumors that retain the wild-type p53 allele. As such, targeted inhibition of Mdm2 is being considered as a therapeutic anticancer strategy. The N-terminal hydrophobic pocket of Mdm2 binds to p53 and t...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2011.625
更新日期:2012-11-08 00:00:00
abstract::Telomerase activation is crucial in human carcinogenesis. The limiting component of telomerase, the catalytic subunit (hTERT), is undetectable in normal somatic cells but present in most tumor cells, including the earliest stages of colon carcinoma. The mechanisms involved in the differential expression in normal and ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1204346
更新日期:2001-05-03 00:00:00
abstract::We have developed a new yeast-based assay for the detection of PTEN nonsense mutation, and applied it to a total of 42 astrocytic tumors. The assay utilizes homologous recombination of PCR-amplified PTEN cDNA samples to a yeast vector which expresses an in-frame PTEN::ADE2 chimera protein. An allele of nonsense mutati...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1203795
更新日期:2000-09-07 00:00:00
abstract::FHIT (Fragile Histidine Triad), a putative tumor suppressor gene, was cloned from fetal brain and colon cDNA libraries. Portions of this gene are deleted in esophageal, colon, lung and breast tumors, but this gene has not been found altered in sporadic renal cell carcinomas. We report here an alternatively spliced for...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201164
更新日期:1997-07-03 00:00:00
abstract::The E6 proteins originating from the tumour-associated Human Papillomavirus (HPV) types 16 and 18 have been shown to bind to and target the tumour suppressor protein, p53, for ubiquitin-mediated degradation. However, in cell lines derived from cervical neoplasias, the predominant early region transcripts are spliced a...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201202
更新日期:1997-07-17 00:00:00
abstract::Matrilysin (MMP-7) is thought to contribute to invasive growth and metastasis of colon carcinoma and many other human cancers. The present study demonstrates that treatment of human colon carcinoma cells with active matrilysin induces cell aggregation in vitro and promotes liver metastasis in nude mice. When two kinds...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1207181
更新日期:2003-11-27 00:00:00
abstract::The Ov/Br septin gene, which is also a fusion partner of MLL in acute myeloid leukaemia, is a member of a family of novel GTP binding proteins that have been implicated in cytokinesis and exocytosis. In this study, we describe the genomic and transcriptional organization of this gene, detailing seventeen exons distrib...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1204752
更新日期:2001-09-13 00:00:00
abstract::Transforming growth factor beta (TGFβ) is a key regulator of epithelial cell proliferation, immune function and angiogenesis. Because TGFβ signaling maintains epithelial homeostasis, dysregulated TGFβ signaling is common in many malignancies, including head and neck squamous cell carcinoma (HNSCC). Defective TGFβ sign...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/onc.2010.306
更新日期:2010-10-07 00:00:00
abstract::Human hematopoietic progenitor cells (TF-1) undergo apoptosis upon deprivation of their dependent cytokine. In this report, we have isolated and characterized some spontaneously derived cytokine-independent variants from TF-1 cells. Analysis of several signaling molecules known to be activated by the GM-CSF pathway re...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1200884
更新日期:1997-02-13 00:00:00
abstract::Arsenite trioxide (As2O3) induces apoptosis in several cell lines by disturbing key signal transduction pathways through its oxidative properties. Here, we report that As2O3 also induces the phosphorylation of the retinoid receptor RXRalpha, subsequent to oxidative damages and the activation of the stress-activated pr...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208402
更新日期:2005-03-31 00:00:00
abstract::A proportion of extraskeletal myxoid chondrosarcomas (EMC) have been shown to have a characteristic translocation t(9;22)(q22;q12) involving the EWS gene at 22q12 and the CHN orphan nuclear receptor gene at 9q22. This translocation appears to be largely specific for EMC, but has not been detected in all such tumours. ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1203156
更新日期:1999-12-09 00:00:00
abstract::The subnuclear distribution of proteins encoded by v-myb and v-myc was analysed in a cell-line of AMV-transformed chicken myeloblasts superinfected by the myc-containing retrovirus MC29. p45v-myb and p110gag-myc, co-expressed in these cells, were released in similar fashion when nuclei were treated with salt or DNAase...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::Tumor suppressor p53 has been shown to repress expression of vascular endothelial growth factor (VEGF), an endothelial cell-specific mitogen and a key mediator of tumor angiogenesis. The p63 gene, recently identified as a p53-relative, encodes multiple isoforms with structural and functional similarities and differenc...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205330
更新日期:2002-04-11 00:00:00
abstract::Uracil in DNA results from deamination of cytosine, resulting in mutagenic U : G mispairs, and misincorporation of dUMP, which gives a less harmful U : A pair. At least four different human DNA glycosylases may remove uracil and thus generate an abasic site, which is itself cytotoxic and potentially mutagenic. These e...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/sj.onc.1205996
更新日期:2002-12-16 00:00:00
abstract::Treatment with retinoic acid (RA) is effective to restore radioactive iodine uptake in metastases of a small fraction of thyroid cancer patients. In order to find predictive markers of response, we took advantage of two thyroid cancer cell lines, FTC133 and FTC238, with low RA-receptor (RAR)beta expression but differi...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210178
更新日期:2007-06-07 00:00:00