Abstract：
AIMS:Alteration of connexin43 (Cx43)-mediated intercellular communication is known to promote susceptibility to ventricular tachyarrhythmias. However, the precise mechanism of the altered Cx43 responsible for arrhythmogenesis remains unclear. We sought to understand changes in impulse propagation of ventricular myocytes under dominant-negative (DN) inhibition of Cx43 in the development of arrhythmias. METHODS AND RESULTS:Intercellular communication was inhibited in confluent monolayers of neonatal rat cultured myocytes by an adenoviral vector-mediated gene transfer for DNCx43-fused red fluorescence protein (RFP). A high-resolution, macro-zoom fluorescence imaging system was used to visualize both the fluo4- and RFP-fluorescence intensities as measures of Ca2+ transient propagation and distribution of DNCx43 inhibition, respectively, in the myocyte monolayers. DNCx43 inhibition of the monolayers resulted in not only a significant slowing of Ca2+ transient propagation velocity, but also a preferential emergence of spiral-wave reentrant arrhythmias elicited by rapid pacing. Detailed observations on the development of spiral waves revealed that the gene-transferred myocyte monolayers exhibited regional slowing of propagation and subsequent generation of wave break, resulting in reentrant arrhythmias. Furthermore, DNCx43-RFP-transferred monolayers showed higher fluorescence intensity of RFP at the break point than at the surrounding myocardium, indicating a culprit role of DNCx43 inhibition in the genesis of spiral reentry. CONCLUSION:The present results indicate that regional heterogeneity in gap-junctional communication promotes, in addition to slowing of conduction velocity, susceptibility to reentrant tachyarrhythmias.
journal_name
Cardiovasc Resjournal_title
Cardiovascular researchauthors
Nakagami T,Tanaka H,Dai P,Lin SF,Tanabe T,Mani H,Fujiwara K,Matsubara H,Takamatsu Tdoi
10.1093/cvr/cvn084subject
Has Abstractpub_date
2008-07-01 00:00:00pages
70-9issue
1eissn
0008-6363issn
1755-3245pii
cvn084journal_volume
79pub_type
杂志文章abstract：OBJECTIVE:To investigate the effect of the ATP-sensitive potassium (KATP) channel inhibitor sodium 5-hydroxydecanoate (5-HD) on the reduction in myocardial infarct size afforded by ischaemic preconditioning in the sodium pentobarbitone rabbit. METHODS:New Zealand white rabbits were anaesthetised with sodium pentobarbi...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：
更新日期：1996-06-01 00:00:00
abstract：:Effects of sublingual glyceryl trinitrate (GTN) were studied in ten patients without heart failure during diagnostic cardiac catheterisation following angiography. GTN caused substantial reduction in peak left ventricular and aortic pressure (19 mmHg) with lesser reduction in mean aortic pressure (9 mmHg) and no chang...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/20.2.153
更新日期：1986-02-01 00:00:00
abstract：:The failing heart is characterized by alterations in energy metabolism, including mitochondrial dysfunction and a reduction in fatty acid (FA) oxidation rate, which is partially compensated by an increase in glucose utilization. Together, these changes lead to an impaired capacity to convert chemical energy into mecha...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1093/cvr/cvn282
更新日期：2009-02-15 00:00:00
abstract：OBJECTIVES:Nitric oxide (NO) has complex effects on myocardial function particularly following ischaemia-reperfusion. The goal of this study was to examine the result of repetitive myocardial stunning on myocardial NO release and expression of inducible (iNOS) and constitutive (eNOS) NO synthases. METHODS AND RESULTS:...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(99)00149-2
更新日期：1999-08-15 00:00:00
abstract：STUDY OBJECTIVE:The aim was to investigate whether basal coronary vascular tone and myocardial perfusion depend upon endothelial nitric oxide (NO) synthesis. DESIGN:Myocardial blood flow and vascular resistance of the left and right ventricles were studied before and after intravenous infusions of either NG-nitro-L-ar...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/25.8.700
更新日期：1991-08-01 00:00:00
abstract：STUDY OBJECTIVE:The aim of the study was to test an intermittent ECG synchronised suction device which was added to a coronary vein retroinfusion system (selective suction and retroinfusion = SSR) in order to transport oxygenated fluid more efficiently into ischaemic myocardium. DESIGN:The suction period preceding the...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/24.6.456
更新日期：1990-06-01 00:00:00
abstract：:Although researchers in radiation and cancer biology have known about the existence of free radicals and their potential role in pathobiology for several decades, cardiac biologists only began to take notice of these noxious species in the 1970s. Exponential growth of free radical research occurred after the discovery...
journal_title：Cardiovascular research
pub_type： 社论,评审
doi：10.1016/s0008-6363(98)00244-2
更新日期：1998-12-01 00:00:00
abstract：OBJECTIVE:The sequelae of myocardial ischaemia can in principle be alleviated by repeated reperfusion, but the accumulation of adenosine monophosphate (AMP) and the loss of interstitial adenosine may lead to adenylate depletion. Repeated oxidative stress could predispose the heart to reperfusion injury. The aim of this...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/26.5.502
更新日期：1992-05-01 00:00:00
abstract：OBJECTIVE:Heart failure is associated with reduced function of sarcoplasmic reticulum (SR) Ca2+-ATPase (SERCA2a) but increased function of sarcolemmal Na+/Ca2+ exchanger (NCX), leading to decreased SR Ca2+ content and loss of frequency-potentiation of contractile force. We reported that SERCA2a-overexpression in transg...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/j.cardiores.2005.05.006
更新日期：2005-09-01 00:00:00
abstract：AIMS:Vascular endothelial growth factor-C (VEGF-C) has been shown to stimulate both angiogenesis and lymphangiogenesis in some but not all models where VEGF-C is over-expressed. Our aim was to investigate the interaction between lymphangiogenesis and angiogenesis in adult tissues regulated by VEGF-C and identify eviden...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvm094
更新日期：2008-05-01 00:00:00
abstract：OBJECTIVE:3-Methylcholanthrene (MC), a polycylic aromatic hydrocarbon, induces atherogenesis in mice fed an atherogenic diet. In this study, we tested the hypothesis that MC would induce DNA adducts in mouse aortic smooth muscle cells (SMCs) and that cytochrome P4501B1 (CYP1B1) plays an important role in the activation...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(01)00536-3
更新日期：2002-03-01 00:00:00
abstract：:Pressure--diameter loops were generated in normal, conscious dogs during left ventricular contraction by means of endocardial ultrasonic gauges and micromanometers. Studies were made in the control state, during volume and pressure loading, and during enhancement of contractility. The diameter at the end of ejection w...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/9.4.447
更新日期：1975-07-01 00:00:00
abstract：:Major advances in molecular biology over the past decade have increased our understanding of how genes are expressed and regulated in mammalian cells in vivo. This knowledge has been translated to investigations of the molecular and cellular biology of the cardiovascular system. Gene transfer, the introduction of a re...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1093/cvr/28.4.445
更新日期：1994-04-01 00:00:00
abstract：:Ventricular function curves relating stroke work and left ventricular end-diastolic pressure were generated in awake dogs during increases in preload produced by infusion of fluid and during increases in afterload produced by administration of phenylephrine. The ventricular function curves produced by preloading were ...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/13.7.392
更新日期：1979-07-01 00:00:00
abstract：Aims:Circular RNA (circRNA) is a newly validated class of single-stranded RNA, ubiquitously expressed in mammalian tissues and possessing key functions including acting as microRNA sponges and as transcriptional regulators by binding to RNA-binding proteins. While independent studies confirm the expression of circRNA i...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvw250
更新日期：2017-03-01 00:00:00
abstract：:Eighty-one consecutive patients representing a wide clinical spectrum were studied to assess the degree of limitation of the atrial extrastimulus technique for measurement of atrioventricular node refractoriness; and, to determine the relationship between conductivity and functional refractoriness of the atrioventricu...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/16.8.457
更新日期：1982-08-01 00:00:00
abstract：OBJECTIVE:The aim was to investigate how platelet activation during myocardial ischaemia can induce electrophysiological and arrhythmogenic effects, and examine the involvement of different platelet membrane receptors in producing these effects. METHODS:Transmembrane action potentials and electrograms were recorded fr...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/28.11.1662
更新日期：1994-11-01 00:00:00
abstract：AIMS:The metabolic syndrome (MS) leads to myocardial fibrosis (MF) and diastolic dysfunction. Statins have proven beneficial effects in MS, but their impact on cardiac remodelling is uncertain. We examined the effects and mechanisms of chronic statin treatment on cardiac remodelling, e.g. fibrosis and diastolic propert...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvt070
更新日期：2013-07-01 00:00:00
abstract：OBJECTIVE:Experimental and clinical studies have shown that administration of insulin during reperfusion is cardioprotective, but the underlying mechanisms are still unknown. In this study, we investigated in isolated rat cardiomyocytes subjected to hypoxia and reoxygenation whether administration of insulin during reo...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/j.cardiores.2006.02.020
更新日期：2006-05-01 00:00:00
abstract：OBJECTIVES:Prior studies have shown that the performance of the left ventricle on any one beat is influenced by the mechanical events of the previous beat, a phenomenon called "previous-beat contraction history". This previous-beat contraction history, which appears to be an interplay between the mechanical events of o...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：OBJECTIVE:In pigs, the infarct size (IS) reduction achieved by a strong preconditioning stimulus (IPs) of 10 min ischemia and 15 min reperfusion is greater than that by a weaker preconditioning stimulus (IPw) of 3 min ischemia and 15 min reperfusion. The cardioprotection achieved by IPw is completely abolished by block...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(01)00505-3
更新日期：2002-08-15 00:00:00
abstract：AIMS:Although increased Na(+)/Ca(2+) exchanger 1 (NCX1) expression is observed during heart failure (HF), the pathological role of NCX1 during the progression of HF remains unclear. We examined alterations of NCX1 expression and activity in hearts after transverse aortic constriction (TAC) surgery and explored whether ...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvw113
更新日期：2016-09-01 00:00:00
abstract：AIMS:Atherosclerotic vascular disease has an inflammatory pathogenesis. Heme from intraplaque hemorrhage may drive a protective and pro-resolving macrophage M2-like phenotype, Mhem, via AMPK and ATF1. The anti-diabetic drug metformin may also activate AMPK-dependent signalling. HYPOTHESIS:Metformin systematically indu...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvaa171
更新日期：2020-07-15 00:00:00
abstract：AIMS:It is well established that leukotrienes (LTs), products of the 5-lipoxygenase (5-LO) pathway, participate in inflammatory tissue reactions and immune responses. In the present study, the impact of the 5-LO pathway on vasculogenesis of mouse embryonic stem (ES) cells was investigated. METHODS AND RESULTS:Immunohi...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvp385
更新日期：2010-04-01 00:00:00
abstract：STUDY OBJECTIVE:The aim was to determine if prostaglandin like activity might be involved in changes due to exercise training in the coronary flow responses to hypoxia. DESIGN:The coronary flow response to hypoxia was measured under constant perfusion pressure in isolated perfused hearts from 12 endurance exercise tra...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/24.9.733
更新日期：1990-09-01 00:00:00
abstract：:Many observational studies and experimental and animal studies have demonstrated that estrogen therapy (ET) or hormone therapy (HT) significantly reduces the risk of coronary heart disease. Nonetheless, recent randomized controlled trials and the Nurses' Health Study in secondary prevention demonstrate trends toward a...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1016/j.cardiores.2005.12.004
更新日期：2006-04-01 00:00:00
abstract：AIMS:Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear receptor superfamily of ligand-activated transcriptional regulators. PPARdelta has an established role in metabolism, wound healing, and angiogenesis. However, little is known about its function in endothelial homeostasis. We investigat...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvp365
更新日期：2010-03-01 00:00:00
abstract：:The G protein-coupled receptor kinases (GRKs) participate with arrestins in the regulation and signal propagation of multiple G protein-coupled receptors (GPCR) of key physiological and pharmacological relevance in the cardiovascular system. The complex mechanisms of regulation of GRK expression, degradation and funct...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1016/j.cardiores.2005.09.011
更新日期：2006-01-01 00:00:00
abstract：:Intracellular acidosis exerts substantial effects on the contractile performance of the heart. Soon after the onset of acidosis, contractility diminishes, largely due to a decrease in myofilament Ca(2+) responsiveness. This decrease in contractility is followed by a progressive recovery that occurs despite the persist...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1016/j.cardiores.2006.12.002
更新日期：2007-03-01 00:00:00
abstract：AIM:To assess whether endothelin-1 (ET-1) induces the in vivo expression of inflammatory-related proteins, namely cyclooxygenase-2 (COX-2) and tissue factor, in the myocardium and circulating leukocytes of guinea-pigs. The involvement of platelets was also analyzed. METHODS:ET-1 (0.013 microg/min) was infused to male ...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(02)00657-0
更新日期：2003-01-01 00:00:00
