Abstract:
:Chlamydia trachomatis is the leading cause of preventable blindness and bacterial sexually transmitted diseases worldwide. Plaque assays have been used to clonally segregate laboratory-adapted C. trachomatis strains from mixed infections, but no assays have been reported to segregate clones from recent clinical samples. We developed a novel shotgun cell culture harvest assay for this purpose because we found that recent clinical samples do not form plaques. Clones were strain-typed by using outer membrane protein A and 16S rRNA sequences. Surprisingly, ocular trachoma reference strain A/SA-1 contained clones of Chlamydophila abortus. C. abortus primarily infects ruminants and pigs and has never been identified in populations where trachoma is endemic. Three clonal variants of reference strain Ba/Apache-2 were also identified. Our findings reflect the importance of clonal isolation in identifying constituents of mixed infections containing new or emerging strains and of viable clones for research to more fully understand the dynamics of in vivo strain-mixing, evolution, and disease pathogenesis.
journal_name
Emerg Infect Disjournal_title
Emerging infectious diseasesauthors
Somboonna N,Mead S,Liu J,Dean Ddoi
10.3201/eid1403.071071subject
Has Abstractpub_date
2008-03-01 00:00:00pages
445-53issue
3eissn
1080-6040issn
1080-6059journal_volume
14pub_type
杂志文章abstract::In Africa, although emergence of multidrug-resistant (MDR) tuberculosis (TB) represents a serious threat in countries severely affected by the HIV epidemic, most countries lack drug-resistant TB data. This finding was particularly true in the Kingdom of Swaziland, which has the world's highest HIV and TB prevalences. ...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1801.110850
更新日期:2012-01-01 00:00:00
abstract::Vaccinia virus (VACV) is a zoonotic agent that causes a disease called bovine vaccinia, which is detected mainly in milking cattle and humans in close contact with these animals. Even though many aspects of VACV infection have been described, much is still unknown about its circulation in the environment and its natur...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid2306.161643
更新日期:2017-06-01 00:00:00
abstract::We aimed to clarify changes in serotypes and genotypes mediating β-lactam and macrolide resistance in Streptococcus pneumoniae isolates from Japanese children who had invasive pneumococcal disease (IPD) after the 7-valent pneumococcal conjugate vaccine (PCV7) was introduced into Japan; 341 participating general hospit...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid2007.131485
更新日期:2014-07-01 00:00:00
abstract::To identify the vectors of bluetongue virus (BTV) in Germany, we monitored Culicoides spp. biting midges during April 2007-May 2008. Molecular characterization of batches of midges that tested positive for BTV suggests C. obsoletus sensu stricto as a relevant vector of bluetongue disease in central Europe. ...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1509.090562
更新日期:2009-09-01 00:00:00
abstract::In winter 2015-16, norovirus GII.17 Kawasaki 2014 emerged as a cause of sporadic gastroenteritis in children in Italy. Median patient age was higher for those with GII.17 than GII.4 infection (55 vs. 24 months), suggesting limited cross-protection for older children. ...
journal_title:Emerging infectious diseases
pub_type: 历史文章,杂志文章
doi:10.3201/eid2307.161255
更新日期:2017-07-01 00:00:00
abstract::Since April 2014, invasive pneumococcal disease incidence has increased substantially across North East England, United Kingdom, reversing the decline that followed the 2006 introduction of pneumococcal conjugate vaccines. Significant increases occurred in 23-valent polysaccharide vaccine serotypes and nonvaccine sero...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid2301.160897
更新日期:2017-01-01 00:00:00
abstract::A sentinel group of primary-care physicians in Wales actively reported cases of acute bloody diarrhea from February 1997 through December 1998. The estimated annual rate was 18 cases per 100,000 population. Most (80%) cases were due to Campylobacter or Salmonella; however, 18% were undiagnosed. ...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid0604.000418
更新日期:2000-07-01 00:00:00
abstract::The mean annual incidence of hemolytic uremic syndrome in persons =15 years of age in Italy from 1988 to 2000 was 0.28 per 100,000 population. Laboratory investigations showed that Shiga toxin-producing Escherichia coli (STEC) infection occurred in 73.1% of patients. STEC O157 was the most common serotype, but a con...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid0901.020266
更新日期:2003-01-01 00:00:00
abstract::Chikungunya virus (CHIKV) was isolated from 12 febrile humans in Yucatan, Mexico, in 2015. One patient was co-infected with dengue virus type 1. Two additional CHIKV isolates were obtained from Aedes aegypti mosquitoes collected in the homes of patients. Phylogenetic analysis showed that the CHIKV isolates belong to t...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid2210.152087
更新日期:2016-10-01 00:00:00
abstract::Hepatitis E virus (HEV) is a zoonotic virus for which pigs are the primary animal reservoir. To investigate whether HEV occurs in mink in Denmark, we screened feces and tissues from domestic and wild mink. Our finding of a novel HEV variant supports previous findings of HEV variants in a variety of species. ...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1912.130614
更新日期:2013-12-01 00:00:00
abstract::We documented a 6-fold increase in the frequency of nontuberculous mycobacteria isolated from clinical samples in Botswana during 2011-2014. Because antituberculosis treatment is often initiated only on the basis of acid-fast bacilli smear-positive microscopy results, some patients with nontuberculous mycobacterial in...
journal_title:Emerging infectious diseases
pub_type: 历史文章,杂志文章
doi:10.3201/eid2507.181440
更新日期:2019-07-01 00:00:00
abstract::Identification and prioritization of effective food safety interventions require an understanding of the relationship between food and pathogen from farm to consumption. Critical to this cause is food attribution, the capacity to attribute cases of foodborne disease to the food vehicle or other source responsible for ...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1107.040634
更新日期:2005-07-01 00:00:00
abstract::Recent epidemiologic, virologic, and modeling reports support the possibility of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission from persons who are presymptomatic (SARS-CoV-2 detected before symptom onset) or asymptomatic (SARS-CoV-2 detected but symptoms never develop). SARS-CoV-2 transmis...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid2607.201595
更新日期:2020-07-01 00:00:00
abstract::We conducted a retrospective population-based study of 140 episodes of Streptococcus dysgalactiae subsp. equisimilis bacteremia occurring in Finland during 1995-2004. Rare emm types were associated with more severe disease and increased mortality rates. Skin and soft tissue infections were more frequent clinical signs...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1605.080803
更新日期:2010-05-01 00:00:00
abstract::Invasive and skin community-associated (CA)-methicillin-resistant Staphylococcus aureus isolates from children were matched with invasive CA-methicillin-sensitive S. aureus strains during 2000-2004. Isolates were analyzed for presence of Panton-Valentine leukocidin. A USA400 lineage clone (n = 6) and the predominant U...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1302.060295
更新日期:2007-02-01 00:00:00
abstract::From January 1982 to May 2000, 17 infections caused by Burkholderia pseudomallei were diagnosed in 15 patients in Taiwan; almost all the infections were diagnosed from 1994 to May 2000. Of the 15 patients, 9 (60%) had underlying diseases, and 10 (67%) had bacteremic pneumonia. Thirteen (76%) episodes of infection were...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid0703.010310
更新日期:2001-05-01 00:00:00
abstract::During May-August 2016, mumps virus genotype K was detected in 12 Vietnam citizens who entered China at the Shuikou border crossing and 1 girl from China. We provide evidence that mumps genotype K is circulating in Vietnam and was imported to China from Vietnam. ...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid2404.170591
更新日期:2018-04-01 00:00:00
abstract::We describe 58 adult patients with melioidosis in Cambodia (2007-2010). Diabetes was the main risk factor (59%); 67% of infections occurred during the rainy season. Bloodstream infection was present in 67% of patients, which represents 12% of all bloodstream infections. The case-fatality rate was 52% and associated wi...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1707.101069
更新日期:2011-07-01 00:00:00
abstract::We found that lethal, urban rat control is associated with a significant increase in the odds that surviving rats carry Leptospira interrogans. Our results suggest that human interventions have the potential to affect and even increase the prevalence of zoonotic pathogens within rat populations. ...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid2402.171371
更新日期:2018-02-01 00:00:00
abstract::Ross River virus, a mosquitoborne alphavirus, causes epidemic polyarthritis in Australia and the Pacific region. We analyzed serum cytokine, chemokine, and growth factor levels in travelers returning to Germany from Australia. Serum samples showed elevated concentrations in the acute phase of the illness and, more pro...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid2304.160466
更新日期:2017-04-01 00:00:00
abstract::Dengue was first reported in Puerto Rico in 1899 and sporadically thereafter. Following outbreaks in 1963 and 1969, the Centers for Disease Control and Prevention has worked closely with the Puerto Rico Department of Health to monitor and reduce the public health burden of dengue. During that time, evolving epidemiolo...
journal_title:Emerging infectious diseases
pub_type: 历史文章,杂志文章
doi:10.3201/eid2508.190089
更新日期:2019-08-01 00:00:00
abstract::Legionella longbeachae, found in soil and compost-derived products, is a globally underdiagnosed cause of Legionnaires' disease. We conducted a case-control study of L. longbeachae Legionnaires' disease in Canterbury, New Zealand. Case-patients were persons hospitalized with L. longbeachae pneumonia, and controls were...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid2307.161429
更新日期:2017-07-01 00:00:00
abstract::Up to 50% of persons with HIV and a diagnosis of tuberculosis (TB) in Thailand die during TB treatment. In a prospective observational study, a team of physicians ascribed the cause of death after reviewing verbal autopsies (interviews of family members about events preceding death), laboratory data, and medical recor...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1502.080942
更新日期:2009-02-01 00:00:00
abstract::The use of polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) to study rpoB gene mutations in rifampin-resistant (RIFr) Mycobacterium tuberculosis has yielded contradictory results. To determine the sensitivity of this method, we analyzed 35 RIFr strains and 11 rifampin-susceptible (RIFs)...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid0706.010615
更新日期:2001-11-01 00:00:00
abstract::Data are missing on the diversity of Plasmodium spp. infecting apes that live in their natural habitat, with limited possibility of human-mosquito-ape exchange. We surveyed Plasmodium spp. diversity in wild chimpanzees living in an undisturbed tropical rainforest habitat and found 5 species: P. malariae, P. vivax, P. ...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1612.100424
更新日期:2010-12-01 00:00:00
abstract::The first two recognized cases of rapidly fatal hantavirus pulmonary syndrome in Pennsylvania occurred within an 8-month period in 1997. Illness in the two patients was confirmed by immunohistochemical techniques on autopsy material. Reverse transcription-polymerase chain reaction analysis of tissue from one patient a...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid0606.000610
更新日期:2000-11-01 00:00:00
abstract::To determine the proportion of Escherichia coli O157 cases in Scotland attributable to secondary spread, we analyzed data obtained through entire-population enhanced surveillance. We identified 11% of cases as secondary. Secondary cases in single households were younger than secondary cases in outbreaks affecting >1 h...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1703.100167
更新日期:2011-03-01 00:00:00
abstract::School closure is a proposed strategy for reducing influenza transmission during a pandemic. Few studies have assessed how families respond to closures, or whether other interactions during closure could reduce this strategy's effect. Questionnaires were administered to 220 households (438 adults and 355 children) wit...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1407.080096
更新日期:2008-07-01 00:00:00
abstract::To assess the incidence of and risk factors for clinical and subclinical dengue virus (DENV) infection, we prospectively studied 1,207 adult short-term travelers from the Netherlands to dengue-endemic areas. Participants donated blood samples for serologic testing before and after travel. Blood samples were tested for...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1705.101125
更新日期:2011-05-01 00:00:00
abstract::Bartonella quintana is a bacterium that causes trench fever in humans. Past reports have shown Bartonella spp. infections in homeless populations in San Francisco, California, USA. The California Department of Public Health in collaboration with San Francisco Project Homeless Connect initiated a program in 2007 to col...
journal_title:Emerging infectious diseases
pub_type: 杂志文章
doi:10.3201/eid1506.090054
更新日期:2009-06-01 00:00:00