rpoB Gene mutations in rifampin-resistant Mycobacterium tuberculosis identified by polymerase chain reaction single-stranded conformational polymorphism.

Abstract:

:The use of polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) to study rpoB gene mutations in rifampin-resistant (RIFr) Mycobacterium tuberculosis has yielded contradictory results. To determine the sensitivity of this method, we analyzed 35 RIFr strains and 11 rifampin-susceptible (RIFs) strains, using the DNA sequencing of the core region of rpoB for comparison. Of the RIFr, 24 had a PCR-SSCP pattern identical to that of H37Rv; the other 11 had four different patterns. The 11 RIFs had PCR-SSCP patterns identical to that of H37Rv. The sensitivity of the assay was 31.4%; its specificity was 100%. We observed a strong correlation between the degree of resistance and the type of mutation.

journal_name

Emerg Infect Dis

authors

Bobadilla-del-Valle M,Ponce-de-Leon A,Arenas-Huertero C,Vargas-Alarcon G,Kato-Maeda M,Small PM,Couary P,Ruiz-Palacios GM,Sifuentes-Osornio J

doi

10.3201/eid0706.010615

subject

Has Abstract

pub_date

2001-11-01 00:00:00

pages

1010-3

issue

6

eissn

1080-6040

issn

1080-6059

journal_volume

7

pub_type

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