Rickettsia prowazekii and real-time polymerase chain reaction.

Abstract:

:Rickettsia prowazekii is the causative agent of epidemic typhus and a potential bioterrorism agent. Sensitive and specific rapid assays are needed to complement existing methods of detecting this organism. We developed a real-time quantitative polymerase chain reaction assay by using a species-specific probe targeting the gltA gene. This assay, which was rapid, specific for R. prowazekii only, and sensitive (cutoff detection of 1 to 5 copies per sample), detected and directly identified R. prowazekii in blood of 12 experimentally infected mice sampled at day 3 and 6 postinfection or in naturally or experimentally infected lice. Because our assay is highly standardized and easily adaptable, it could improve epidemic typhus surveillance in public health programs, especially for countries with underdiagnosed or unrecognized human cases.

journal_name

Emerg Infect Dis

authors

Svraka S,Rolain JM,Bechah Y,Gatabazi J,Raoult D

doi

10.3201/eid1203.050888

subject

Has Abstract

pub_date

2006-03-01 00:00:00

pages

428-32

issue

3

eissn

1080-6040

issn

1080-6059

journal_volume

12

pub_type

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