Abstract：
:To increase knowledge of the pathogenic potential of the Burkholderia cepacia complex (BCC), we investigated the effects of reference strains of the nine BCC species on human bronchial epithelial cells in vitro. B. multivorans exhibited the highest rates of adherence to and internalization by host cells. Two out of three clinical isolates recovered from cystic fibrosis patients confirmed the B. multivorans high adhesiveness. All four B. multivorans isolates exhibited an aggregated pattern of adherence but any of them expressed cable pili. When bacteria were centrifuged onto cell cultures to circumvent their poor adhesiveness, B. pyrrocinia exhibited the highest internalization rate, followed by B. multivorans. The percentages of apoptotic cells in cultures infected with B. cepacia, B. multivorans, B. cenocepacia (subgroups IIIA and IIIB), B. stabilis and B. vietnamiensis were significantly higher than in control non-infected cultures. All nine BCC species triggered a similar release of the inflammatory cytokine IL-8, that was not reduced by cell treatment with cytochalasin D. Hence, our data demonstrate, for the first time, that all BCC species exhibit a similar ability to induce the expression of host immune mediators whereas they differ on their ability to adhere to, invade and kill airway epithelial cells.
journal_name
Microbes Infectjournal_title
Microbes and infectionauthors
Moura JA,Cristina de Assis M,Ventura GC,Saliba AM,Gonzaga L Jr,Si-Tahar M,Marques Ede A,Plotkowski MCdoi
10.1016/j.micinf.2007.10.002subject
Has Abstractpub_date
2008-01-01 00:00:00pages
52-9issue
1eissn
1286-4579issn
1769-714Xpii
S1286-4579(07)00313-9journal_volume
10pub_type
杂志文章abstract：:Influenza A virus (IAV) is a pulmonary pathogen, responsible for significant yearly morbidity and mortality. Due to the absence of highly effective antiviral therapies and vaccine, as well as the constant threat of an emerging pandemic strain, there is considerable need to better understand the host-pathogen interacti...
journal_title：Microbes and infection
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journal_title：Microbes and infection
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journal_title：Microbes and infection
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journal_title：Microbes and infection
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journal_title：Microbes and infection
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journal_title：Microbes and infection
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journal_title：Microbes and infection
pub_type： 杂志文章
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
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journal_title：Microbes and infection
pub_type： 杂志文章
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更新日期：2015-11-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
doi：10.1016/j.micinf.2005.08.002
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2010.12.003
更新日期：2011-04-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
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更新日期：2012-09-01 00:00:00
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journal_title：Microbes and infection
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2005.11.011
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journal_title：Microbes and infection
pub_type： 杂志文章
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journal_title：Microbes and infection
pub_type： 杂志文章
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更新日期：2011-08-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
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更新日期：2006-06-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
doi：10.1016/j.micinf.2009.08.012
更新日期：2009-11-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/s1286-4579(00)00318-x
更新日期：2000-04-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
doi：10.1016/s1286-4579(00)90364-2
更新日期：2000-06-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2006.02.019
更新日期：2006-06-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
doi：10.1016/s1286-4579(99)80063-x
更新日期：1999-07-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2005.10.020
更新日期：2006-04-01 00:00:00
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/s1286-4579(00)00366-x
更新日期：2000-05-01 00:00:00
