Usefulness of flow cytometry in the diagnosis of mycosis fungoides.

Abstract:

BACKGROUND:The pathologic evaluation of mycosis fungoides (MF) is a challenging area in dermatopathology. OBJECTIVE:We sought to determine the usefulness of flow cytometry for the diagnosis of MF from skin biopsy specimens. METHODS:Skin biopsy specimens from 22 patients with a clinical suggestion for MF were evaluated by 4-color flow cytometry. The results were correlated with the International Society for Cutaneous Lymphoma (ISCL) MF diagnostic score and molecular studies for T-cell receptor gene rearrangement. RESULTS:A T-cell abnormality by flow cytometry was identified in all 11 patients with diagnostic ISCL scores whereas the 7 patients with either subdiagnostic ISCL scores or reactive histology showed no phenotypic abnormality by flow cytometry. In all, 10 of 11 patients with diagnostic skin biopsy specimens for MF had T-cell receptor gene rearrangements by polymerase chain reaction. Gene rearrangements were not detected in the subdiagnostic group. LIMITATIONS:Small study size was a limitation. CONCLUSION:Flow cytometry of skin biopsy specimens is a sensitive method for detecting abnormalities in MF and should be considered part of the routine workup of patients with a clinical suggestion of MF.

journal_name

J Am Acad Dermatol

authors

Oshtory S,Apisarnthanarax N,Gilliam AC,Cooper KD,Meyerson HJ

doi

10.1016/j.jaad.2007.01.017

subject

Has Abstract

pub_date

2007-09-01 00:00:00

pages

454-62

issue

3

eissn

0190-9622

issn

1097-6787

pii

S0190-9622(07)00202-2

journal_volume

57

pub_type

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