Abstract:
:The complete genomic sequence of an isolate of Tobacco vein banding mosaic virus (TVBMV-YND) from Yunnan, China was determined by sequencing overlapping cDNA fragments obtained by RT-PCR with degenerate and/or specific primers. The genome is composed of 9,570 nucleotides (nt) excluding the 3'-terminal poly (A) tail and contains one single open reading frame of 9,240 nt encoding a large polyprotein of 3,079 amino acids with predicted Mr of 348.6 kDa. Phylogenetic analysis of complete genomic sequences confirmed that TVBMV is a distinct species of the genus Potyvirus. Different parts of TVBMV-YND genome shared different levels of identity with other species of potyviruses, while most parts showed greatest identity with Chilli veinal mottle virus among the potyviruses with available complete genomic sequences. TVBMV-YND had a rare Q/N cleavage site for NIb/CP and uncommon RITC motif in HC-Pro that is crucial for aphid transmission of potyviruses.
journal_name
Virus Genesjournal_title
Virus genesauthors
Yu XQ,Lan YF,Wang HY,Liu JL,Zhu XP,Valkonen JP,Li XDdoi
10.1007/s11262-007-0135-7subject
Has Abstractpub_date
2007-12-01 00:00:00pages
801-6issue
3eissn
0920-8569issn
1572-994Xjournal_volume
35pub_type
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