Abstract:
:Monoclonal antibodies for targeting cytotoxic conjugates to tumor cells are currently being evaluated together with extracorporeal affinity adsorption. The aim of the adsorption was to reduce undesired side effects in normal organs and to increase the tumor-to-normal tissue ratios. This technique is also applicable to several other therapeutic areas such as immune-mediated disorders, that is, autoimmunity, allergy, and transplantation rejection. We describe an improved technique for extracorporeal affinity adsorption of radiolabeled biotinylated antibodies in rats. Blood access is established through the tail artery and tail vein, without surgical insertion of permanent catheters. This technique is simple, does not require surgery, and causes only minimal stress to the animals. In addition, experiments can be carried out on several animals simultaneously. This new technique is of considerable benefit for studying extracorporeal affinity adsorption in rats, as experiments can be carried out with negligible anatomical and physiological interventions, compared to previously used techniques.
journal_name
Artif Organsjournal_title
Artificial organsauthors
Mårtensson L,Nilsson R,Sjögren HO,Strand SE,Tennvall Jdoi
10.1111/j.1525-1594.2007.00380.xsubject
Has Abstractpub_date
2007-04-01 00:00:00pages
312-6issue
4eissn
0160-564Xissn
1525-1594pii
AOR380journal_volume
31pub_type
杂志文章abstract::The purified water supplies and randomly selected dialysates of 51 chronic and acute dialysis centers in the central United States were surveyed to assess the relative risks to dialysis patients from microbial and endotoxin contamination. A culture medium more sensitive than those generally employed in routine quality...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1990.tb01601.x
更新日期:1990-04-01 00:00:00
abstract::Characteristics of morphology, cardiac function, and cardiac reserve at late state were evaluated at rest, by changing the pacing rates, and after exercise in patients with ventricular pacemakers for isolated congenital complete atrioventricular block. Heart size was reduced statistically after pacemaker implantation,...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1987.tb02667.x
更新日期:1987-06-01 00:00:00
abstract::The abdominal left ventricular assist device (ALVAD) is an order of magnitude more effective than conventional intra-aortic balloon pumping (IABP) in unloading and providing circulatory support to the failing left ventricle. This is a report of a unique case which demonstrates that in the absence of pulmonary vascular...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1978.tb01632.x
更新日期:1978-11-01 00:00:00
abstract::Bovine pericardium is a widely utilized biomaterial. Usually, after harvesting, it is advantageous that the pericardium be immersed in glycerol to improve its shelf life. This can induce some degree of toxicity in the material. The studies were performed in compliance with the rules of ISO 10993 and OECD 487, in the b...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2008.00542.x
更新日期:2008-04-01 00:00:00
abstract::The purpose of this paper is to review the pathways of hydrogen ion production and removal due to intermediary metabolism. The principal method of analysis employed was one based on a theoretical approach because it is the most accurate and has the broadest application. In the absence of hypoxia and insulin deficiency...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1982.tb04126.x
更新日期:1982-11-01 00:00:00
abstract::Since the introduction of high-performance prosthetic heart valves, particularly bileaflet valves and monoleaflet valves with larger opening angles, we have observed in vivo complex leaflet motion which has not been experimentally confirmed yet. We developed a computer-controlled hydraulic mock circulator to study the...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1997.tb00373.x
更新日期:1997-04-01 00:00:00
abstract::As a preliminary investigation to establish a cryopreservation method suited for bioartificial livers (BALs), three-dimensional (3-D) cryopreservation experiments with fibroblasts were performed, in which the cells were firstly seeded into a porous scaffold, and the scaffold containing the cells was then cryopreserved...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2009.00933.x
更新日期:2010-07-01 00:00:00
abstract::A comparative study of the thrombogenicity of several microporous membranes was performed using an ex vivo system simulating the conditions in an artificial lung. A study of platelet adhesion and adsorbed protein layer indicated that the membranes separated into three groups. Copolyurethane membranes adsorbed very few...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1985.tb04343.x
更新日期:1985-02-01 00:00:00
abstract::Individualized short hemodialysis treatment schedules, approximately three 3-h treatments per week, were introduced in a nephrology unit in 1976. In May 1985, 259 patients had been treated, which corresponds to approximately 60 new patients per million inhabitants per year. Ages at start of therapy ranged between 17 a...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1986.tb02541.x
更新日期:1986-06-01 00:00:00
abstract::Electrolyzed water accelerated the healing of full-thickness cutaneous wounds in rats, but only anode chamber water (acid pH or neutralized) was effective. Hypochlorous acid (HOCl), also produced by electrolysis, was ineffective, suggesting that these types of electrolyzed water enhance wound healing by a mechanism un...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.2000.06557-3.x
更新日期:2000-12-01 00:00:00
abstract::The generation of anaphylatoxins, particularly C5a, is important in extracorporeal circulation therapies such as granulocyte/monocyte apheresis, which activates the complement system and elevates C5a levels. However, no side effects of granulocyte/monocyte apheresis using cellulose acetate beads have been reported. To...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2009.00995.x
更新日期:2010-12-01 00:00:00
abstract::In cardiac surgeries, it is frequently necessary to make the external blood circulation and oxygenation artificially. This procedure is called extracorporeal circulation (EC) or heart-lung bypass. During EC, one of the most important parameters, which demands continuous monitoring, is the blood flow. In many cases, th...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.2000.06532.x
更新日期:2000-03-01 00:00:00
abstract::We aimed to evaluate the timing of preoperative intra-aortic balloon pump (IABP) placement and outcomes in patients undergoing off-pump coronary artery bypass grafting (OPCAB). Patients with prophylactic IABP placement before OPCAB presenting between January 1, 2010 and December 31, 2013 were included. Patients were c...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.13009
更新日期:2018-03-01 00:00:00
abstract::Success of total artificial heart (TAH) implantation as a temporary measure depends on three factors: proper patient selection, adequate surgical technique, and safe cardiac prostheses. From 1986 until March 1990, 237 patients with stagnant heart failure were examined. Sixty-one patients (27%) were put on a waiting li...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1991.tb00745.x
更新日期:1991-10-01 00:00:00
abstract::ABO incompatible allogeneic bone marrow transplants can be performed successfully to treat patients with leukemia or aplastic anemia. These transplants carry no great risk of rejection or graft-versus-host disease, however, some method must be used to avoid acute hemolysis at the time of infusion of ABO incompatible m...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1981.tb03998.x
更新日期:1981-08-01 00:00:00
abstract::There has been increasing interest in the development of a hepatocyte bioreactor for the treatment of acute hepatic failure; however, little is known about the effect of hepatocyte byproducts on the viability of the cells in the bioreactor environment. We investigated the effects of increasing concentrations of bile o...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.1998.06088.x
更新日期:1998-10-01 00:00:00
abstract::Pharmacologic agents and other non-protein-bound compounds smaller than 5,000 daltons have the potential to be removed by continuous arteriovenous hemofiltration (CAVH). A proposed method for estimating drug clearance by CAVH (ClCAVH) equates ultrafiltrate clearance to the product of the sieving coefficient and the av...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1990.tb02997.x
更新日期:1990-12-01 00:00:00
abstract::Fibroblast cell culture was performed to evaluate cell adhesion and cell morphology on novel hydrolyzable copolymers composed of poly(D,L-lactic acid) (PDLA) macromonomer, 2-methacryloyloxyethyl phosphorylcholine (MPC), and n-butyl methacrylate. The copolymers were used as cell culture materials for regulating the int...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.2003.07015.x
更新日期:2003-03-01 00:00:00
abstract::Left ventricular assist devices (LVADs) are increasingly being used as life-saving therapy in patients with end-stage heart failure. The changes in spirometry following LVAD implantation and subsequent unloading of the left ventricle and pulmonary circulation are unknown. In this study, we explored long-term changes i...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.12507
更新日期:2015-12-01 00:00:00
abstract::The injurious effects of reactive oxygen species on venous tissues and the potential protective role played by green tea polyphenol (GTPP) on human saphenous veins were investigated. Oxidative stress was induced exogenously in the vein segments, either by adding 0.8 or 1.6 M of H2O2, or by using 80 or 160 U/L of xanth...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2003.07163.x
更新日期:2003-12-01 00:00:00
abstract::From January 1980 through December 1990, implantation of a permanent pacemaker was performed in 34 patients following open heart surgery. The patients were divided into two groups according to the clinical indications for implantation of permanent pacemakers. Group 1 (9 patients) had surgical atrioventricular block, a...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1993.tb00591.x
更新日期:1993-05-01 00:00:00
abstract::The presence in rat serum of three novel immunosuppressive proteins is associated with induction of tolerance after liver grafting. One of these, KX-5 (40 kilodalton molecular weight) is present 40-100 days after grafting of DA liver into PVG, a combination in which liver grafts are not rejected; two others, KX-2 (87 ...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1996.tb00646.x
更新日期:1996-10-01 00:00:00
abstract::Temporary and immediately usable vascular access is a vital need in treating uremic patients. Subclavian vein cannulation, although a major progress, has been associated with significant morbidity and mortality. Accordingly, for the last 2.5 years the authors have been developing an alternative technique consisting of...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1986.tb02587.x
更新日期:1986-10-01 00:00:00
abstract::Over a 4 year period, five of 98 patients at our dialysis unit developed signs and symptoms consistent with first-use syndrome (FUS). Marked improvement was noted after subjecting new dialyzers to automated processing using either formaldehyde or peracetic acid. No episodes of FUS occurred in patients being treated wi...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1987.tb02645.x
更新日期:1987-04-01 00:00:00
abstract::Our aim was to study the aging effects on the in vitro biological properties of bone marrow-derived mesenchymal stem cells (BMSCs) for construction of tissue-engineered heart valves. BMSCs were taken from teenagers with congenital heart diseases, and middle-aged and elderly patients with valvular diseases. Proliferati...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2009.00824.x
更新日期:2010-03-01 00:00:00
abstract::Atherosclerosis is accelerated in dialysis patients, but less is known about asymptomatic atherosclerosis and major risk factors in patients with different stages of chronic kidney disease (CKD). We compared intima media thickness (IMT) and plaque occurrence in the carotid arteries in 104 nondiabetic patients (stages ...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2007.00522.x
更新日期:2008-03-01 00:00:00
abstract::The E4T is a totally implantable total artificial heart (TAH) resulting from many years of research work at the Cleveland Clinic Foundation (CCF) and Nimbus, Inc. It consists of four implanted subsystems: the pumping unit, the variable volume device, the transcutaneous transformer, and the internal battery. The pumpin...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1988.tb02795.x
更新日期:1988-10-01 00:00:00
abstract::Neurologic complications during on-pump cardiovascular surgery are often induced by mobilization of atherosclerotic plaques, which is directly related to enhanced wall shear stress. In the present study, we numerically evaluated the impact of dispersive aortic cannulas on aortic blood flow characteristics, with specia...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.12359
更新日期:2015-03-01 00:00:00
abstract::As the aortic valve (AV) opens, the pump pressure head remains constant, which is reflected as a "notch/plateau" in pump pressure and flow signals. However, instantaneous flow estimation may be influenced by friction and is particularly difficult in axial pumps. Therefore, a new method to determine the duration of AV ...
journal_title:Artificial organs
pub_type: 杂志文章,多中心研究
doi:10.1111/aor.12570
更新日期:2015-08-01 00:00:00
abstract::This study introduces a newly developed method for the formation of a stationary phase superficially embedded with immunoadsorbent, that is partially incomplete two-stage polymerization of acrylamide. Preliminary experimental studies show that binding activity of the immunoadsorbent was satisfactorily maintained after...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2005.29071.x
更新日期:2005-05-01 00:00:00