当前位置： SCI文献检索 > CLINICAL AND EXPERIMENTAL IMMUNOLOGY期刊下所有文献 > Pre-incubation with interleukin-4 mediates a direct protective effect against the loss of pancreatic beta-cell viability induced by proinflammatory cytokines.

Pre-incubation with interleukin-4 mediates a direct protective effect against the loss of pancreatic beta-cell viability induced by proinflammatory cytokines.

Abstract：

:Loss of pancreatic beta-cells in type I diabetes is associated with an increase in T helper 1 (Th1) proinflammatory cytokines in the islet milieu, with a concomitant reduction in Th2 anti-inflammatory cytokines. In animal models, manoeuvres designed to polarize Th1 responses towards Th2, particularly involving interleukin (IL)-4, have been shown to protect against insulitis and diabetes. The aim of this study was to determine whether IL-4 can exert a direct effect on beta-cell viability. The rat pancreatic beta-cell line, BRIN-BD11, was used. IL-4R mRNA expression was assayed by reverse transcription-polymerase chain reaction and DNA sequencing and protein expression measured using anti-IL-4R antibodies and confocal microscopy. Cells were pretreated in vitro with IL-4, incubated with IL-1beta and interferon (IFN)-gamma and DNA fragmentation and nitrite production analysed by flow cytometry and Griess assay, respectively. Expression of type I (IL-4R alpha and common gamma-chain) and type II (IL-4R alpha, IL-13R alpha-1) IL-4R mRNA transcripts, together with cell surface expression of IL-4R, was demonstrated. Pre-incubation with IL-4 reduced significantly cell death induced by IL-1beta alone or by a combination of IL-1beta and IFN-gamma, although this was not accompanied by a reduced production of nitrite. The protective effect of IL-4 was not seen when all three cytokines were added simultaneously. These results demonstrate, for the first time, expression of IL-4 receptor components on rat pancreatic beta-cells and reveal a direct protective effect on the loss of viability mediated by proinflammatory cytokines when beta-cells are pre-incubated with IL-4.

journal_name

Clin Exp Immunol

journal_title

Clinical and experimental immunology

authors

Kaminski A,Kaminski ER,Morgan NG

doi

10.1111/j.1365-2249.2007.03375.x

subject

Has Abstract

pub_date

2007-06-01 00:00:00

pages

583-8

issue

3

eissn

0009-9104

issn

1365-2249

pii

CEI3375

journal_volume

148

pub_type

杂志文章

相关文献

CLINICAL AND EXPERIMENTAL IMMUNOLOGY文献大全
  • T cell vaccination in multiple sclerosis patients with autologous CSF-derived activated T cells: results from a pilot study.

    abstract：:Myelin-reactive T cells are considered to play an essential role in the pathogenesis of multiple sclerosis (MS), an autoimmune disease of the central nervous system. We have previously studied the effects of T cell vaccination (TCV), a procedure by which MS patients are immunized with attenuated autologous myelin basi...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.2003.02019.x

    authors： Van der Aa A,Hellings N,Medaer R,Gelin G,Palmers Y,Raus J,Stinissen P

    更新日期：2003-01-01 00:00:00

  • Endothelial tissue factor stimulation by proteinase 3 and elastase.

    abstract：:In ANCA-associated vasculitis the activation of primed leucocytes by autoantibodies with subsequent release of proteases such as myeloperoxidase (MPO), proteinase 3 (PR3) and elastase is thought to play an important pathogenetic role. Whether these proteases contribute to the vascular lesions by stimulating the procoa...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.2001.01587.x

    authors： Haubitz M,Gerlach M,Kruse HJ,Brunkhorst R

    更新日期：2001-12-01 00:00:00

  • Humoral immune responses in periodontal disease may have mucosal and systemic immune features.

    abstract：:The humoral immune response, especially IgG and IgA, is considered to be protective in the pathogenesis of periodontal disease, but the precise mechanisms are still unknown. Immunoglobulins arriving at the periodontal lesion are from both systemic and local tissue sources. In order to understand better the local immun...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.1999.00819.x

    authors： Kinane DF,Lappin DF,Koulouri O,Buckley A

    更新日期：1999-03-01 00:00:00

  • Induction and increase of HLA-DR antigen expression by immune interferon on ML-3 cell line enhances the anti-HLA-DR immunotoxin activity.

    abstract：:In order to evaluate the impact of induction and increase target antigen expression on immunotoxin potency, we measured the potentiating effect of recombinant immune interferon-gamma (rIFN-gamma) on the cytotoxicity of an anti HLA-DR ricin A-chain immunotoxin (2G5 RTA-IT) on the myeloid cell line ML-3. After 48 h of i...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1990.tb05429.x

    authors： Chiron M,Jaffrezou JP,Carayon P,Bordier C,Roubinet F,Xavier C,Brandely M,Laurent G

    更新日期：1990-11-01 00:00:00

  • An abnormal adherence of monocytes to fibronectin in thyroid autoimmunity has consequences for cell polarization and the development of veiled cells.

    abstract：:Blood monocytes of patients with thyroid autoimmune disease (TAID) display defects in rearranging their cortical actomyosin cytoskeleton ('polarize') in response to chemoattractants. Such rearrangements also take place after the adherence of monocytes to the extracellular matrix (ECM). It is therefore not surprising t...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.2001.01583.x

    authors： Canning MO,Grotenhuis K,De Haan-Meulman M,De Wit HJ,Berghout A,Drexhage HA

    更新日期：2001-07-01 00:00:00

  • Antigen specific correlations of cellular immune responses in human leishmaniasis suggests mechanisms for immunoregulation.

    abstract：:Regulation of the immune response directed against Leishmania is critical for the establishment of effective control of the disease. It is likely that some types of immune responses directed against Leishmania can lead to more severe clinical forms of leishmaniasis causing a poor control of the pathogen and/or patholo...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2004.02426.x

    authors： Antonelli LR,Dutra WO,Almeida RP,Bacellar O,Gollob KJ

    更新日期：2004-05-01 00:00:00

  • Emerging viruses and current strategies for vaccine intervention.

    abstract：:During the past decade several notable viruses have suddenly emerged from obscurity or anonymity to become serious global health threats, provoking concern regarding their sustained epidemic transmission in immunologically naive human populations. With each new threat comes the call for rapid vaccine development. Inde...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章,评审

    doi：10.1111/cei.13295

    authors： Afrough B,Dowall S,Hewson R

    更新日期：2019-05-01 00:00:00

  • Relationship of granulocyte colony stimulating factor with other acute phase reactants in man.

    abstract：:The non-specific acute phase response in mice is associated with increased resistance to bacterial infection, which is critically mediated by granulocyte colony stimulating factor (G-CSF), but the behaviour of G-CSF in the human acute phase response is not known. Cardiothoracic surgery is a powerful acute phase stimul...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2005.02732.x

    authors： Noursadeghi M,Pepys MB,Gallimore R,Cohen J

    更新日期：2005-04-01 00:00:00

  • Thyroglobulin antibodies in Graves' disease are associated with T-cell receptor beta chain and major histocompatibility complex loci.

    abstract：:We have investigated the T-cell antigen receptor constant beta and alpha chain genes (TCR-C beta, -C alpha) and the immunoglobulin (Ig) heavy chain switch regions of patients with Graves' disease (GD) using restriction fragment length polymorphism (RFLP) analysis. No significant associations were found with RFLPs of e...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Demaine AG,Ratanachaiyavong S,Pope R,Ewins D,Millward BA,McGregor AM

    更新日期：1989-07-01 00:00:00

  • Altered polyvinylpyrrolidone clearance and immune responsiveness caused by small dietary changes.

    abstract：:The introduction of 25 mg of a new protein antigen into the diets of mice produced transient increases in PVP clearance which reached a maximum 60 hr after the change of diet and had disappeared by 4 days. At the same time the immune responsiveness to unrelated antigens given parenterally was also raised transiently, ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Newby TJ,Stokes CR,Bourne FJ

    更新日期：1980-02-01 00:00:00

  • Monocyte chemoattractant protein-1 (MCP-1) inhibits the intestinal-like differentiation of monocytes.

    abstract：:Monocytes (MO) migrating into normal, non-inflamed intestinal mucosa undergo a specific differentiation resulting in a non-reactive, tolerogenic intestinal macrophage (IMAC). Recently we demonstrated the differentiation of MO into an intestinal-like macrophage (MAC) phenotype in vitro in a three-dimensional cell cultu...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2006.03113.x

    authors： Spoettl T,Hausmann M,Herlyn M,Gunckel M,Dirmeier A,Falk W,Herfarth H,Schoelmerich J,Rogler G

    更新日期：2006-07-01 00:00:00

  • Reactivity of presumed anti-natural killer cell antibody Leu 7 with intrafollicular T lymphocytes.

    abstract：:This report describes the presence of a T lymphocyte subpopulation in germinal centres of lymph follicles. This subpopulation is defined by reactivity with Leu 7 antibody, in addition to OKT11, OKT1, OKT3 and OKT4 positivity. The functional activity of this T lymphocyte subpopulation is a matter of discussion and has ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Poppema S,Visser L,De Leij L

    更新日期：1983-12-01 00:00:00

  • Complement membrane attack is required for endplate damage and clinical disease in passive experimental myasthenia gravis in Lewis rats.

    abstract：:Myasthenia gravis (MG) is a debilitating and potentially fatal neuromuscular disease characterized by the generation of autoantibodies reactive with nicotinic acetylcholine receptors (AChR) that cause loss of AChR from the neuromuscular endplate with resultant failure of neuromuscular transmission. A role for compleme...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2006.03198.x

    authors： Chamberlain-Banoub J,Neal JW,Mizuno M,Harris CL,Morgan BP

    更新日期：2006-11-01 00:00:00

  • Allogenic induction of thromboplastin synthesis in monocytes and endothelial cells. Biphasic effect of cyclosporin A.

    abstract：:Monocytes and endothelial cells were stimulated in co-culture with allogeneic lymphocytes to produce thromboplastin (TPL). The induction was biphasic, an early response (8-24 h) was greatly augmented by cyclosporin A (CS) (0.5-5 micrograms/ml) whereas the late response (day 3-4) was inhibited. Prednisolone inhibited b...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Carlsen E,Gaudernack G,Filion-Myklebust C,Pettersen KS,Prydz H

    更新日期：1989-06-01 00:00:00

  • Humoral and cell-mediated immune responses in chronic typhoid carriers.

    abstract：:Humoral and cell-mediated immune responses to Salmonella typhi were studied in 10 chronic typhoid carriers, and in healthy controls. Carriers showed impaired cellular reactivity to S. typhi antigens in the leucocyte migration inhibition test (LMI). Carriers did not show a generalized depression of cell-mediated immuni...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Dham SK,Thompson RA

    更新日期：1982-10-01 00:00:00

  • Differential responses of human dendritic cells to metabolites from the oral/airway microbiome.

    abstract：:Small molecule metabolites that are produced or altered by host-associated microbial communities are emerging as significant immune response modifiers. However, there is a key gap in our knowledge of how oral microbial metabolites affect the immune response. Here, we examined the effects of metabolites from five bacte...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/cei.12943

    authors： Whiteson K,Agrawal S,Agrawal A

    更新日期：2017-06-01 00:00:00

  • Infiltrating cellular pattern in kidney graft biopsies translates into forkhead box protein 3 up-regulation and p16INK4α senescence protein down-regulation in patients treated with belatacept compared to cyclosporin A.

    abstract：:Renal allograft survival is related directly to cell senescence. In the transplantation scenario many cellular events - participating as immunological and non-immunological factors - could contribute to accelerate this biological process, responsible for the ultimate fate of the graft. Mechanisms concerned in toleranc...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2011.04504.x

    authors： Furuzawa-Carballeda J,Lima G,Alberú J,Palafox D,Uribe-Uribe N,Morales-Buenrostro LE,Reyes Acevedo R,Mondragón G,Chevaile A,Llorente L

    更新日期：2012-02-01 00:00:00

  • Activation of c-Jun N-terminal kinase (JNK) signalling in experimentally induced gastric lesions in rats.

    abstract：:The c-Jun N-terminal kinase (JNK) participates in intracellular signalling cascades that mediate inflammatory responses. Therefore, the JNK signalling may be involved in gastric injury and inhibition of this pathway may form the basis of a new strategy for the treatment of gastric injury. The aim of this study was to ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2005.02959.x

    authors： Mitsuyama K,Tsuruta O,Matsui Y,Harada K,Tomiyasu N,Suzuki A,Takaki K,Masuda J,Handa K,Satoh Y,Bennett BL,Toyonaga A,Sata M

    更新日期：2006-01-01 00:00:00

  • Reactivity of normal rat epiphyseal chondrocytes with monoclonal antibodies recognizing different leucocyte markers.

    abstract：:The aim of the study was to test whether normal rat epiphyseal chondrocytes react with monoclonal antibodies (MoAbs) detecting different markers of lymphoid cells. For this purpose, isolated chondrocyte and, for comparison, splenocyte cytosmears were exposed to a battery of different MoAbs followed by indirect immunop...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Malejczyk J,Romaniuk A

    更新日期：1989-03-01 00:00:00

  • Specific antibody deficiency in children with recurrent respiratory infections: a controlled study with follow-up.

    abstract：:Specific antibody deficiency (SAD) to unconjugated pneumococcal vaccine (PPV) is an established primary B cell immunodeficiency. The occurrence and natural history of SAD in children is unclear. We conducted an observational study to identify SAD in children with recurrent respiratory infections. Ninety-nine children,...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/cei.12053

    authors： Ruuskanen O,Nurkka A,Helminen M,Viljanen MK,Käyhty H,Kainulainen L

    更新日期：2013-05-01 00:00:00

  • Isolation and characterization of dendritic cells from adenoids of children with otitis media with effusion.

    abstract：:Dendritic cells were enriched from adenoids of children with otitis media with effusion (OME) by density gradient centrifugation and culture techniques. An enrichment of 40-140-fold was obtained for dendritic cells. These cells were identified using morphology, enzyme cytochemistry, immunocytochemistry and functional ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1992.tb03085.x

    authors： van Nieuwkerk EB,van der Baan S,Richters CD,Kamperdijk EW

    更新日期：1992-05-01 00:00:00

  • Bullous pemphigoid blister fluid stimulates eosinophil colony formation and activates eosinophils.

    abstract：:An eosinophil stimulating material (ESM) is described in the blister fluids (BF) of six patients with bullous pemphigoid (BP). This ESM is similar in function to a subspecies of human colony stimulating factor (CSF), CSF-alpha, since (i) it stimulated the production from bone marrow cells of day 14 colonies (31 +/- 15...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Varigos GA,Morstyn G,Vadas MA

    更新日期：1982-12-01 00:00:00

  • The micro-sperm immobilization test: the use of only motile spermatozoa and studies of complement.

    abstract：:A technique is described which shows how to obtain a suspension of only progressively motile spermatozoa, from semen, by active penetration into an overlying buffer layer. About 60% of the number of motile spermatozoa in the original semen sample can be obtained as an output in the removed buffer layer. With this meth...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Hellema HW,Rümke P

    更新日期：1978-01-01 00:00:00

  • Human intestinal epithelial cell-derived interleukin (IL)-18, along with IL-2, IL-7 and IL-15, is a potent synergistic factor for the proliferation of intraepithelial lymphocytes.

    abstract：:Intestinal epithelial cell (IEC)-derived cytokines, such as stem cell factor (SCF), interleukin (IL)-7 and IL-15 are known to be required for the development of intestinal intraepithelial lymphocytes (IELs). A newly described cytokine, IL-18, has also been shown to be produced by intestinal epithelial cells. To demons...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2004.02431.x

    authors： Okazawa A,Kanai T,Nakamaru K,Sato T,Inoue N,Ogata H,Iwao Y,Ikeda M,Kawamura T,Makita S,Uraushihara K,Okamoto R,Yamazaki M,Kurimoto M,Ishii H,Watanabe M,Hibi T

    更新日期：2004-05-01 00:00:00

  • CD5-positive B cells after T cell depleted bone marrow transplantation.

    abstract：:CD5 is an antigen normally found on T cells and on a minority subpopulation of B cells in fetal spleen and tonsil and on the majority of cells in B-chronic lymphocytic leukaemia. Recent reports described the occurrence of large numbers of CD5-positive B cells in the peripheral blood after bone marrow transplantation (...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Drexler HG,Brenner MK,Wimperis JZ,Gignac SM,Janossy G,Prentice HG,Hoffbrand AV

    更新日期：1987-06-01 00:00:00

  • Regulation of immunoglobulin synthesis by monocytes in multiple myeloma and benign monoclonal gammopathy.

    abstract：:The distribution and regulatory function of immunoglobulin (Ig) synthesis by monocytes were assessed using several monoclonal antibodies and a cell sorter in samples from 13 patients with multiple myeloma (MM) and nine patients with benign monoclonal gammopathy (BMG). Since all of these cells were separated directly w...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Takeshita A

    更新日期：1986-12-01 00:00:00

  • Anti-proteinase 3 antibodies, their characterization and disease associations.

    abstract：:Anti-proteinase 3 antibodies are a subgroup of anti-neutrophil cytoplasmic antibodies (ANCA), and we have established an ELISA for their detection using high performance liquid chromatography (HPLC)-purified protein. This assay is sensitive and specific: inhibition studies have shown that despite the homology between ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1994.tb06519.x

    authors： Jennings JG,Chang L,Savige JA

    更新日期：1994-02-01 00:00:00

  • Monocytes show immunoregulatory capacity on CD4+ T cells in a human in-vitro model of extracorporeal photopheresis.

    abstract：:Extracorporeal photopheresis (ECP) is a widely used immunomodulatory therapy for the treatment of various T cell-mediated disorders such as cutaneous T cell lymphoma (CTCL), graft-versus-host disease (GvHD) or systemic sclerosis. Although clinical benefits of ECP are already well described, the underlying mechanism of...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/cei.13232

    authors： Wiese F,Reinhardt-Heller K,Volz M,Gille C,Köstlin N,Billing H,Handgretinger R,Holzer U

    更新日期：2019-03-01 00:00:00

  • Interleukin 12B gene polymorphism and apparent resistance to hepatitis C virus infection.

    abstract：:Cellular immunity with interferon gamma production could have a role in protection from hepatitis C virus (HCV). Interleukin (IL)-12 is a key cytokine in promoting such anti-viral T helper 1 (Th1) responses. We hypothesized that a genetic background able to promote cellular responses may be associated with apparent pr...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2008.03655.x

    authors： Hegazy D,Thurairajah P,Metzner M,Houldsworth A,Shaw S,Kaminski E,Demaine AG,Cramp ME

    更新日期：2008-06-01 00:00:00

  • Limitations of the semisynthetic library approach for obtaining human monoclonal autoantibodies to the thyrotropin receptor of Graves' disease.

    abstract：:Graves' disease (GD) is characterized by the presence of autoantibodies against the TSH-receptor (TSH-R) which are pathogenic and, upon binding to the receptor, trigger intracellular signal transduction. The autoantibodies are oligoclonal and as they are responsible for disease activity, their characterization would l...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.1999.01042.x

    authors： Van Der Heijden JH,De Bruin TW,Glaudemans KA,De Kruif J,Banga JP,Logtenberg T

    更新日期：1999-11-01 00:00:00