Insulin-stimulated NADH/NAD+ redox state increases NAD(P)H oxidase activity in cultured rat vascular smooth muscle cells.


BACKGROUND:We reported that insulin modestly stimulates NAD(P)H oxidase activity in cultured rat vascular smooth muscle cells (VSMC) and synergistically stimulates enzyme activity with angiotensin II (Ang II), leading to synergistic stimulation of VSMC migration. The aim of this study was to determine the mechanism of insulin-stimulated NAD(P)H oxidase activity. METHODS:Cultured rat VSMC O(2)(-) and H(2)O(2) production was measured by lucigenin and luminol luminescence, respectively, and lactate and pyruvate content of cell lysates determined by measuring increase or loss, respectively, of NADH in the presence of lactate dehydrogenase. Migration of VSMC was determined by a wound closure method. RESULTS:Administration of 1 nmol/L insulin increased the lactate/pyruvate ratio (LPR), and hence the NADH/NAD(+) ratio by 122 +/- 16% (P < .05). Exogenous lactate (5 mmol/L), which increased the LPR similarly to insulin, increased O(2)(-) production by 123% +/- 32% (P < .05); Ang II (50 nmol/L) increased it by 60% +/- 9% (P < .05); but together these agents synergistically increased O(2)(-) production to 343% +/- 72% above the control value (P < .05 v the sum of the lactate-and Ang II-stimulated values), all in a diphenyleneiodonium-sensitive or apocynin-sensitive manner. Blocking the increase of insulin in the LPR with exogenous pyruvate or oxaloacetate blocked insulin's stimulation and insulin's plus Ang II's synergistic stimulation of O(2)(-)production as well as insulin's stimulation of migration of Ang II-treated VSMC. Insulin plus Ang II also stimulated H(2)O(2) production. Neither wortmannin nor pertussis toxin, which inhibit insulin-stimulated NAD(P)H oxidase activity in other cell types, affected insulin or insulin plus Ang II-stimulated O(2)(-)production. CONCLUSIONS:Insulin stimulates NAD(P)H oxidase activity and with Ang II synergistically stimulates it in cultured rat VSMC by increasing the NADH/NAD(+) redox potential, but not by phosphatidylinositol 3-kinase or heterotrimeric G(iota) protein-dependent pathways.


Am J Hypertens


Yang M,Kahn AM




Has Abstract


2006-06-01 00:00:00














  • High Home Blood Pressure Variability Associates With Exaggerated Blood Pressure Response to Cold Stress.

    abstract:BACKGROUND:Exaggerated sympathetic cardiovascular (CV) reactivity to stress associates with elevated risk for clinical and preclinical end points of CV disease. It would be useful to identify these individuals, preferably from feasible measurements commonly used in health care. Our study examined the association betwee...

    journal_title:American journal of hypertension

    pub_type: 杂志文章


    authors: Hintsala HE,Kiviniemi AM,Antikainen R,Mäntysaari M,Jokelainen J,Hassi J,Tulppo MP,Herzig KH,Keinänen-Kiukaanniemi S,Rintamäki H,Jaakkola JJK,Ikäheimo TM

    更新日期:2019-05-09 00:00:00

  • Should Blood Pressure Targets After Lacunar Stroke Vary by Body Size? The SPS3 Trial.

    abstract:BACKGROUND:It is unknown whether the physiological impact of a given blood pressure (BP) varies by body size. We explored interactions between higher vs. lower systolic BP (SBP) targets and anthropometric measures (body mass index (BMI), body surface area (BSA), height, weight) and recurrent stroke and death in the Sec...

    journal_title:American journal of hypertension

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Field TS,McClure LA,White CL,Pergola PE,Hart RG,Benavente OR,Hill MD,SPS3 Investigators.

    更新日期:2015-06-01 00:00:00

  • Is endothelial function of the radial artery altered in human essential hypertension?

    abstract::There is controversy over whether endothelial function is impaired in human essential hypertension. All studies to date have used measurements of forearm blood flow by plethysmography to assess endothelium-dependent vasodilation and endothelial function. In contrast to these studies, which have focused on resistance v...

    journal_title:American journal of hypertension

    pub_type: 临床试验,杂志文章


    authors: Schmieder RE,Weihprecht H,Schobel H,John S,Weidinger G,Gatzka C,Veelken R

    更新日期:1997-03-01 00:00:00

  • Angiotensin II subtype-1 receptor antagonists improve hemodynamic and renal changes without affecting glucose metabolisms in genetic rat model of non-insulin-dependent diabetes mellitus.

    abstract::The Otsuka Long-Evans Tokushima Fatty (OLETF) rat is a new genetic model of non-insulin-dependent diabetes mellitus (NIDDM). We investigated whether treatment with an angiotensin II (ANGII) subtype-1 receptor antagonist delays the onset of NIDDM and attenuates diabetic nephropathy in the OLETF rat. OLETF rats fed a re...

    journal_title:American journal of hypertension

    pub_type: 杂志文章


    authors: Uehara Y,Hirawa N,Kawabata Y,Numabe A,Gomi T,Ikeda T,Omata M

    更新日期:1999-01-01 00:00:00

  • Effect of education on blood pressure control in elderly persons: a randomized controlled trial.

    abstract:BACKGROUND:It is not clear which educational strategy is most effective in helping patients to change their lifestyles. This study compared the efficacy of two different educational models on reducing blood pressure (BP). METHODS:This was a randomized controlled trial in ambulatory hypertensive patients >65 years of a...

    journal_title:American journal of hypertension

    pub_type: 杂志文章,随机对照试验


    authors: Figar S,Galarza C,Petrlik E,Hornstein L,Rodríguez Loria G,Waisman G,Rada M,Soriano ER,de Quirós FG

    更新日期:2006-07-01 00:00:00

  • Increased Circulating Visfatin Is Associated With Progression of Kidney Disease in Non-Diabetic Hypertensive Patients.

    abstract:BACKGROUD:Declining renal function is an independent risk factor for all-cause mortality in cardiovascular disease. Visfatin has been described as a marker of inflammation and endothelial dysfunction, but whether circulating visfatin levels are predictive to a subsequent decline in renal function remains unclear. METH...

    journal_title:American journal of hypertension

    pub_type: 杂志文章


    authors: Hsu CY,Huang PH,Chen TH,Chiang CH,Leu HB,Huang CC,Chen JW,Lin SJ

    更新日期:2016-04-01 00:00:00

  • Glomerular Density in Biopsy-Proven Hypertensive Nephrosclerosis.

    abstract:BACKGROUND:Previous autopsy studies suggested that a reduced nephron number is associated with increased risk of hypertension and chronic kidney disease. However, the significance of the nephron number estimated from a renal biopsy in patients with hypertensive nephrosclerosis (HNS) has not yet been elucidated. METHOD...

    journal_title:American journal of hypertension

    pub_type: 杂志文章


    authors: Haruhara K,Tsuboi N,Kanzaki G,Koike K,Suyama M,Shimizu A,Miyazaki Y,Kawamura T,Ogura M,Yokoo T

    更新日期:2015-09-01 00:00:00

  • Effects of isradipine on peripheral hemodynamic reflex responses in mild-to-moderate essential hypertension.

    abstract::In a randomized double-blind, placebo-controlled, crossover study of isradipine (5 mg twice daily), effects on peripheral hemodynamic reflex responses were studied in nine patients (mean age 48 years) at baseline and after six weeks of active treatment. Assessments included vital signs, resting blood flow in the calf ...

    journal_title:American journal of hypertension

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Duprez D,De Backer T,De Pue N,Hermans L,De Buyzere M,Clement DL

    更新日期:1991-02-01 00:00:00

  • The essence of effective treatment and compliance is simplicity.

    abstract::In recent years, several international bodies of experts have established extensive guidelines for the prevention and treatment of coronary heart disease (CHD) that clearly define the importance of altering patients' lifestyles and administering therapeutic agents that positively affect disease progression. However, s...

    journal_title:American journal of hypertension

    pub_type: 杂志文章,评审


    authors: Erhardt LR

    更新日期:1999-10-01 00:00:00

  • Hyperinsulinemia, insulin resistance, and hyperglycemia: contributing factors in the pathogenesis of hypertension and atherosclerosis.

    abstract::Subtle abnormalities of carbohydrate metabolism and overt diabetes mellitus are both associated with a substantial increase in the prevalence of hypertension and the accelerated development of atherosclerosis. Hypertension is also a presumed independent risk factor for atherosclerosis, although some of the atherogenic...

    journal_title:American journal of hypertension

    pub_type: 杂志文章,评审


    authors: Sowers JR,Standley PR,Ram JL,Jacober S,Simpson L,Rose K

    更新日期:1993-07-01 00:00:00

  • Birth measurements, family history, and environmental factors associated with later-life hypertensive status.

    abstract:BACKGROUND:This birth cohort study was conducted to investigate the contribution of prenatal and antenatal environmental exposures to later-life hypertensive status. METHODS:Two thousand five hundred and three individuals born in 1921-1954 at the Peking Union Medical College Hospital (PUMCH) were targeted; 2,081 (83.1...

    journal_title:American journal of hypertension

    pub_type: 杂志文章


    authors: Chen X,Zhang ZX,George LK,Wang ZS,Fan ZJ,Xu T,Zhou XL,Han SM,Wen HB,Zeng Y

    更新日期:2012-04-01 00:00:00

  • Association of Orthostatic Hypotension Timing With Clinical Events in Adults With Diabetes and Hypertension: Results From the ACCORD Trial.

    abstract:OBJECTIVE:To determine the effects of orthostatic hypotension (OH) measurement timing on its associations with dizziness, falls, fractures, cardiovascular disease (CVD), and mortality. METHODS:We analyzed OH measurements from the Action to Control Cardiovascular Risk in Diabetes BP trial, which evaluated two blood pre...

    journal_title:American journal of hypertension

    pub_type: 杂志文章


    authors: Juraschek SP,Lipsitz LA,Beach JL,Mukamal KJ

    更新日期:2019-06-11 00:00:00

  • G-6A polymorphism of angiotensinogen gene modulates the effect of blood pressure on carotid intima-media thickness. The Bogalusa Heart Study.

    abstract:BACKGROUND:The adverse association between blood pressure and carotid artery intima-media thickness (IMT), a surrogate measure of subclinical atherosclerosis, is well-known. However, whether the G-6A polymorphism of the angiotensinogen (AGT) gene, a candidate gene of hypertension and vascular remodeling, modulates this...

    journal_title:American journal of hypertension

    pub_type: 杂志文章


    authors: Bhuiyan AR,Chen W,Srinivasan SR,Rice J,Mock N,Tang R,Bond MG,Boerwinkle E,Berenson GS

    更新日期:2007-10-01 00:00:00

  • LASSBio-294, A compound with inotropic and lusitropic activity, decreases cardiac remodeling and improves Ca²(+) influx into sarcoplasmic reticulum after myocardial infarction.

    abstract:BACKGROUND:Myocardial infarction (MI) is commonly associated with cardiac hypertrophy, reduced Ca²(+) uptake into the sarcoplasmic reticulum (SR) and impaired myocardial relaxation. Treatment to prevent MI-associated complications is currently lacking. The purpose of the present study was to investigate the remodeling ...

    journal_title:American journal of hypertension

    pub_type: 杂志文章


    authors: Costa DG,da Silva JS,Kümmerle AE,Sudo RT,Landgraf SS,Caruso-Neves C,Fraga CA,de Lacerda Barreiro EJ,Zapata-Sudo G

    更新日期:2010-11-01 00:00:00

  • Cardioprotective Effects of Paricalcitol Alone and in Combination With FGF23 Receptor Inhibition in Chronic Renal Failure: Experimental and Clinical Studies.

    abstract:BACKGROUND:In uremic animals, vitamin D receptor (VDR) agonists like paricalcitol (Pc) attenuate cardiac hypertrophy, but this effect has not been replicated consistently in humans with chronic kidney disease. Elevated fibroblast growth factor 23 (FGF23) levels cause cardiac hypertrophy with activation of the myocardia...

    journal_title:American journal of hypertension

    pub_type: 杂志文章


    authors: Czaya B,Seeherunvong W,Singh S,Yanucil C,Ruiz P,Quiroz Y,Grabner A,Katsoufis C,Swaminathan S,Abitbol C,Rodriguez-Iturbe B,Faul C,Freundlich M

    更新日期:2019-01-01 00:00:00

  • Effect of antihypertensive monotherapy and combination therapy on arterial distensibility and left ventricular mass.

    abstract:BACKGROUND:Angiotensin-converting enzyme (ACE) inhibitors and calcium channel blockers (CCBs) increase arterial compliance and decrease left ventricular mass in hypertensive patients. This study examined whether combined therapy has greater arterial and cardiac effects than doubled doses of the individual drugs. METHO...

    journal_title:American journal of hypertension

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Neutel JM,Smith DH,Weber MA

    更新日期:2004-01-01 00:00:00

  • Efficacy of low-dose spironolactone in subjects with resistant hypertension.

    abstract:BACKGROUND:Previous reports have demonstrated the antihypertensive efficacy of high doses of spironolactone in subjects with primary aldosteronism and, to a lesser degree, subjects with resistant hypertension. METHODS:In current analysis, we examined the antihypertensive benefit of adding low-dose spironolactone to mu...

    journal_title:American journal of hypertension

    pub_type: 临床试验,杂志文章


    authors: Nishizaka MK,Zaman MA,Calhoun DA

    更新日期:2003-11-01 00:00:00

  • Does evidence-based medicine suggest that physicians should not be measuring blood pressure in the hypertensive patient?

    abstract::The most common reason for an outpatient physician visit is for the diagnosis and treatment of hypertension. The Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure (JNC) VII, which is increasingly evidence-based, advises the clinician to use studies of the mean response...

    journal_title:American journal of hypertension

    pub_type: 社论,评审


    authors: Graves JW,Sheps SG

    更新日期:2004-04-01 00:00:00

  • Association between adducin-1 G460W variant and blood pressure in Swedes is dependent on interaction with body mass index and gender.

    abstract:BACKGROUND:The W allele of the G460W polymorphism in the adducin-1 gene has been occasionally associated with increased blood pressure (BP). The aim of this study was to test whether the G460W variant is associated with BP levels and BP progression rate and whether G460W associations with BP are affected by sex, body m...

    journal_title:American journal of hypertension

    pub_type: 杂志文章


    authors: Fava C,Montagnana M,Almgren P,Rosberg L,Guidi GC,Berglund G,Melander O

    更新日期:2007-09-01 00:00:00

  • Effect of surgical treatment on hypertension in Cushing's syndrome.

    abstract::The effect of a surgical cure of hypercortisolism on hypertension in 54 patients with Cushing's syndrome was assessed. The correlation between preoperative duration of hypertension and posttreatment blood pressure was significant (P < .01). Restoration of normal cortisol was associated with blood pressure normalizatio...

    journal_title:American journal of hypertension

    pub_type: 临床试验,杂志文章


    authors: Fallo F,Sonino N,Barzon L,Pistorello M,Pagotto U,Paoletta A,Boscaro M

    更新日期:1996-01-01 00:00:00

  • alpha-Tocopherol increased nitric oxide synthase activity in blood vessels of spontaneously hypertensive rats.

    abstract::Antioxidant protection provided by different doses of alpha-tocopherol was compared by determining nitric oxide synthase (NOS) activity in blood vessels of spontaneously hypertensive rats (SHR) treated with alpha-tocopherol. SHR were divided into four groups namely hypertensive control (C), treatment with 17 mg of alp...

    journal_title:American journal of hypertension

    pub_type: 杂志文章


    authors: Newaz MA,Nawal NN,Rohaizan CH,Muslim N,Gapor A

    更新日期:1999-08-01 00:00:00

  • Effects of estradiol and progesterone on platelet calcium responses.

    abstract::We investigated the effects of 17 beta-estradiol (beta E2), alpha-estradiol (alpha E2), and progesterone (P) on baseline and vasopressin (AVP)-induced [Ca2+]i in human platelets obtained from healthy male and female volunteers. Platelets were treated with beta E2, alpha E2, P, or ethanol vehicle for 30 min at 37 degre...

    journal_title:American journal of hypertension

    pub_type: 杂志文章


    authors: Raman BB,Standley PR,Rajkumar V,Ram JL,Sowers JR

    更新日期:1995-02-01 00:00:00

  • Hypertension in primary hyperparathyroidism--reduction of blood pressure by long-term treatment with vitamin D (alphacalcidol). A double-blind, placebo-controlled study.

    abstract::Patients with primary hyperparathyroidism (HPT) often have raised blood pressure but a simple cause-and-effect relationship has not been established. In 33 persons with probable primary HPT and mild hypercalcemia detected in a health survey, diastolic blood pressure (DBP) was significantly higher than among age- and s...

    journal_title:American journal of hypertension

    pub_type: 临床试验,杂志文章


    authors: Lind L,Wengle B,Wide L,Sörensen OH,Ljunghall S

    更新日期:1988-10-01 00:00:00

  • Fixed-dose combination therapy with trandolapril and verapamil SR is effective in primary hypertension. Trandolapril Study Group.

    abstract::We assessed the efficacy of monotherapy with trandolapril, an angiotensin converting enzyme (ACE) inhibitor, and of verapamil slow-release (SR), a calcium antagonist, each in a range of three doses as monotherapy, and in the nine possible combinations of therapy in patients with stage I to III diastolic hypertension. ...

    journal_title:American journal of hypertension

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: DeQuattro V,Lee D

    更新日期:1997-07-01 00:00:00

  • Methods and devices for measuring arterial compliance in humans.

    abstract::This review analyses methods and devices used worldwide to evaluate the arterial stiffness. Three main methodologies are based upon analysis of pulse transit time, of wave contour of the arterial pulse, and of direct measurement of arterial geometry and pressure, corresponding to regional, systemic and local determina...

    journal_title:American journal of hypertension

    pub_type: 杂志文章,评审


    authors: Pannier BM,Avolio AP,Hoeks A,Mancia G,Takazawa K

    更新日期:2002-08-01 00:00:00

  • Gender-specific correlates of leptin with hypertension-related phenotypes in African Americans.

    abstract::Leptin may be a link in the relationship of obesity with hypertension. We evaluated associations of leptin with blood pressure (BP) in 54 normotensive and 114 hypertensive African American individuals. Plasma leptin was higher (P <.03) in hypertensive women than in normotensive women, although body mass index did not ...

    journal_title:American journal of hypertension

    pub_type: 杂志文章


    authors: El-Gharbawy AH,Kotchen JM,Grim CE,Kaldunski M,Hoffmann RG,Pausova Z,Hamet P,Kotchen TA

    更新日期:2002-11-01 00:00:00

  • Pressor and bradycardic effects of centrally administered relaxin in conscious rats.

    abstract::The current study tested the hypothesis that centrally administered relaxin elevates arterial pressure in conscious rats and that this hypertensive effect is mediated, at least in part, by central or peripheral vasopressin. Injection of human relaxin (0.068 or 0.34 microgram in 200 nL artificial cerebrospinal fluid) i...

    journal_title:American journal of hypertension

    pub_type: 杂志文章


    authors: Yang RH,Bunting S,Wyss JM,Berecek KH,Zhang L,Jin H

    更新日期:1995-04-01 00:00:00

  • Association study of the elastin microfibril interfacer 1 (EMILIN1) gene in essential hypertension.

    abstract:BACKGROUND:Elastin microfibril interfacer 1 (EMILIN-1) is a negative regulator of the transforming growth factor-beta (TGF-beta) signaling, which is involved in blood pressure (BP) homeostasis. Emilin1 knockout mice display elevated BP. The aim of the present study was to assess the association between the human EMILIN...

    journal_title:American journal of hypertension

    pub_type: 杂志文章


    authors: Shimodaira M,Nakayama T,Sato N,Naganuma T,Yamaguchi M,Aoi N,Sato M,Izumi Y,Soma M,Matsumoto K

    更新日期:2010-05-01 00:00:00

  • Changes of plasma endothelin and growth factor levels, and of left ventricular mass, after chronic AT1-receptor blockade in human hypertension.

    abstract::The stimulation of autocrine and paracrine factors such as basic fibroblast- (bFGF) and platelet-derived (PDGF) growth factors mediates many of the growth-promoting actions of angiotensin II. The aim of this study was to evaluate the effect of chronic AT1-receptor blockade on plasma endothelin-1 (ET-1) and growth fact...

    journal_title:American journal of hypertension

    pub_type: 杂志文章


    authors: Cottone S,Vadalà A,Vella MC,Nardi E,Mulé G,Contorno A,Riccobene R,Cerasola G

    更新日期:1998-05-01 00:00:00

  • Captopril renography. Early observations and diagnostic criteria.

    abstract::This article describes the methods and the interpretation of captopril renography. The essentials of the methods are the discontinuation of diuretics, the refraining from solid foods before administration of 25 mg captopril, hydration to induce diuresis, the performing of 1-min sequential images, data acquisition with...

    journal_title:American journal of hypertension

    pub_type: 杂志文章,评审


    authors: Oei HY

    更新日期:1991-12-01 00:00:00