Abstract:
:The aim of this work was to identify spectral markers of cell proliferation that could be of use in clinical MRS. Cultured C6 ATCC rat glioma cells were used as models for this purpose and metabolites were extracted with perchloric acid at three different growth curve stages: log, confluence and post-confluence. 1D and 2D in vitro(1)H NMR spectra were recorded at 9.4 T. Statistically significant changes in myo-inositol and glutamine concentrations between log phase and post-confluence were found when normalized to the creatine ratio. The myo-inositol/creatine ratio was 2.76 +/- 0.82 at log phase increasing to 7.43 +/- 1.34 at post-confluence, while the glutamine/creatine ratio decreased from 0.22 +/- 0.03 to 0.10 +/- 0.02. No significant differences were recorded for other metabolites investigated. The fact that both myo-inositol and glutamine are detectable by in vivo MRS at clinical fields makes their changes relevant as potential astrocytic tumour proliferation rate markers in clinical MRS.
journal_name
NMR Biomedjournal_title
NMR in biomedicineauthors
Valverde D,Quintero MR,Candiota AP,Badiella L,Cabañas ME,Arús Cdoi
10.1002/nbm.1024subject
Has Abstractpub_date
2006-04-01 00:00:00pages
223-30issue
2eissn
0952-3480issn
1099-1492journal_volume
19pub_type
杂志文章abstract::Increased lactate production through glycolysis in aerobic conditions is a hallmark of cancer. Some anticancer drugs have been designed to exploit elevated glycolysis in cancer cells. For example, lonidamine (LND) inhibits lactate transport, leading to intracellular acidification in cancer cells. Chemical exchange sat...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3287
更新日期:2015-05-01 00:00:00
abstract::To assess infarction development in pig hearts, Mn-enhanced and Gd-enhanced MRI were used. In domestic pigs (25-35 kg, n = 37), the first and second diagonal branches of the left anterior descending coronary artery were ligated to induce acute ischemia and infarction (ischemia+reperfusion) or chronic infarction of inc...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1297
更新日期:2009-02-01 00:00:00
abstract::Phosphorus-31 magnetic resonance spectroscopy ((31)P-MRS) has gained much interest in schizophrenia research in recent years since it allows the non-invasive measurement of high-energy phosphates and phospholipids in vivo. However, until now only differences in metabolite concentrations between certain brain areas of ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/(sici)1099-1492(199912)12:8<483::aid-nbm58
更新日期:1999-12-01 00:00:00
abstract::Non-alcoholic steatohepatitis (NASH) is characterized at histology by steatosis, hepatocyte ballooning and inflammatory infiltrates, with or without fibrosis. Although diamagnetic material in fibrosis and inflammation can be detected with quantitative susceptibility imaging, fatty acid composition changes in NASH rela...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3766
更新日期:2017-10-01 00:00:00
abstract::Most ovarian tumors are cystic structures containing variable amounts of fluid. Several studies of ovarian cyst fluid focus on one specific metabolite using conventional assay systems. We examined the potential of (1)H-nuclear magnetic resonance spectroscopy in evaluation of the overall metabolic composition of cyst f...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/1099-1492(200008)13:5<297::aid-nbm648>3.0.
更新日期:2000-08-01 00:00:00
abstract::Elevated brain myo-inositol (m-Ins) concentration (a putative marker of neuroinflammation) has been reported in patients suffering from type 2 diabetes mellitus (T2DM). Obesity alone and T2DM have been found to be associated with a lower concentration of N-acetyloaspartate and N-acetylaspartylglutamate (tNAA, a marker...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3957
更新日期:2018-09-01 00:00:00
abstract::Cerebral ischemia provokes tissue damage by two major patho-physiological mechanisms. Direct cell necrosis is induced by diminished access of neurons and glia to essential nutrients such as glucose and oxygen leading to energy failure. A second factor of cellular loss is related to the activation of immune-competent c...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.770
更新日期:2002-06-01 00:00:00
abstract::Chemical exchange saturation transfer (CEST)-derived, pH-weighted, amide proton transfer (APT) MRI has shown promise in animal studies for the prediction of infarction risk in ischemic tissue. Here, APT MRI was translated to patients with acute stroke (1-24 h post-symptom onset), and assessments of APT contrast, perfu...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3048
更新日期:2014-02-01 00:00:00
abstract::Metabolic activity in the lung is known to change in response to external insults, inflammation, and cancer. We report measurements of metabolism in the isolated, perfused rat lung of healthy controls and in diseased lungs undergoing acute inflammation using hyperpolarized 1-(13) C-labeled pyruvate. The overall appare...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3139
更新日期:2014-08-01 00:00:00
abstract::Diffusion tensor MRI (DTI) is a method for the noninvasive assessment of cellular organization and integrity in vivo. In this study, in vivo DTI was performed to demonstrate its ability to reflect photoreceptor cell alignment in adult C57BL/6 wild-type mice. Age-matched retinal degeneration 1 (rd1) mice were employed ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1609
更新日期:2011-06-01 00:00:00
abstract::Obesity causes damage to several organs, including the brain. Recent studies have been focusing on understanding the mechanisms through which obesity affects brain structure and function using neuroimaging techniques. A functional biomarker, such as cerebral blood flow (CBF), is a powerful tool that can be used to exp...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.4375
更新日期:2020-10-01 00:00:00
abstract::Aminoacylase 1 deficiency is a novel inborn error of metabolism. The clinical significance of the deficiency is under discussion, as well as the possible consequences of the defect for brain metabolism and function. This study includes the five originally published cases as well as three novel ones. NMR spectroscopy o...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1170
更新日期:2008-02-01 00:00:00
abstract::Hyperglycemia (blood glucose concentration >150 mg/dL) is common in extremely low gestational age newborns (ELGANs; birth at <28 week gestation). Hyperglycemia increases the risk of brain injury in the neonatal period. The long-term effects are not well understood. In adult rats, hyperglycemia alters hippocampal energ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3910
更新日期:2018-05-01 00:00:00
abstract::This review highlights various magnetic resonance image (MRI) segmentation algorithms that employ pattern recognition. The procedures are grouped into two categories: low- to intermediate-level, and high-level image processing. The former consists of grey level histogram analysis, texture definition, edge identificati...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/(sici)1099-1492(199806/08)11:4/5<157::aid-
更新日期:1998-06-01 00:00:00
abstract::Resolution enhancement for glutamate (Glu), glutamine (Gln) and glutathione (GSH) in the human brain by TE-optimized point-resolved spectroscopy (PRESS) at 7 T is reported. Sub-TE dependences of the multiplets of Glu, Gln, GSH, γ-aminobutyric acid (GABA) and N-acetylaspartate (NAA) at 2.2-2.6 ppm were investigated wit...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1529
更新日期:2010-11-01 00:00:00
abstract::In this study, metabolic changes in the 79A/C3H, ME7/VM, ME7/C3H, 87V/VM and 22A/SV scrapie mouse models were investigated during the clinical phase of the disease, using in vitro proton nuclear magnetic resonance spectroscopy. N-acetyl-aspartate was found to be significantly reduced in infected mice of the ME7/ C3H (...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/(SICI)1099-1492(199612)9:8<359::AID-NBM428
更新日期:1996-12-01 00:00:00
abstract::A noise figure and noise parameter measurement system was developed that consists of a combination spectrum and network analyzer, preamplifier, programmable power supply, noise source, tuning board, and desktop computer. The system uses the Y-factor method for noise figure calculation and allows calibrations to correc...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3138
更新日期:2014-08-01 00:00:00
abstract::Here, we show that the sensitivity of (31)P MRSI of (31)P spins J-coupled to protons can be increased by almost a factor of three when compared with an optimal direct detection free induction decay. By direct detection integrated with multi-echo polarization transfer (DIMEPT), multiple signals from polarization transf...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3183
更新日期:2014-10-01 00:00:00
abstract::Changes in physiological parameters such as cerebral blood flow, cerebral blood volume, oxygen extraction, and the size and distribution of cerebral blood vessels, result in changes in the local concentration of deoxyhaemoglobin ([dHb]). The purpose of this study was to quantitatively investigate the dependence of the...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/(sici)1099-1492(199810)11:6<281::aid-nbm52
更新日期:1998-10-01 00:00:00
abstract::Normal subjects performed voluntary, isometric exercise 1 s contraction, 1 s rest for 10 min) of the first dorsal interosseous (FDI) muscle with a target force of 25, 50 and 100% of the maximal voluntary contraction (MVC) force. 31P NMR spectra were collected continuously before, during and after exercise. Data were a...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940030504
更新日期:1990-10-01 00:00:00
abstract::The purpose of this study was to investigate the effect of gadolinium (III) diethyltriaminepenta-acetic acid (Gd-DTPA) mixed with a fixative on the image contrast between the white and gray matter of the perfusion-fixed mouse brain. A series of microscopic MRI (microMRI) studies using different concentrations of Gd-DT...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1324
更新日期:2009-04-01 00:00:00
abstract::The measurement of pulmonary perfusion (blood delivered to the capillary bed within a voxel) using arterial spin labeling (ASL) magnetic resonance imaging is often complicated by signal artifacts from conduit vessels that carry blood destined for voxels at a distant location in the lung. One approach to dealing with c...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3358
更新日期:2015-09-01 00:00:00
abstract::Spontaneous fluctuations in the blood oxygenation level-dependent (BOLD) MRI signal during the resting state are increasingly being studied in healthy and diseased brain in humans and animal models. Yet, the relationship between functional brain status and the characteristics of spontaneous BOLD fluctuations remains p...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1556
更新日期:2011-01-01 00:00:00
abstract::Central obesity is the hallmark of a number of non-inheritable disorders. The advent of imaging techniques such as MRI has allowed for a fast and accurate assessment of body fat content and distribution. However, image analysis continues to be one of the major obstacles to the use of MRI in large-scale studies. In thi...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3432
更新日期:2015-12-01 00:00:00
abstract::MR spectroscopic imaging (MRSI) at ultra-high field (≥7 T) benefits from improved sensitivity that allows the detection of low-concentration metabolites in the brain. However, optimized acquisition techniques are required to overcome inherent limitations of MRSI at ultra-high field. This work describes an optimized me...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3950
更新日期:2018-11-01 00:00:00
abstract::Despite 40 years of NMR spectroscopy and 10 years of in vivo spectroscopy in humans, the break-even point for routine clinical diagnosis has not yet been reached. Obviously there are many factors that stimulate but also restrict the use of proton NMR spectroscopy in clinical routine. ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940070803
更新日期:1994-12-01 00:00:00
abstract::Results are presented from an in vivo study of human skin in which a zig-zag surface coil together with a three-pulse Fourier Series Window (FSW) protocol have been used to obtain minimally contaminated 31P skin spectra of the posterior calf. Phantom experiments indicate that while the fall-off in the B1 field profile...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/(SICI)1099-1492(199608)9:5<195::AID-NBM416
更新日期:1996-08-01 00:00:00
abstract::We review several methods that have been developed to infer microstructural and physiological information about isotropic and anisotropic tissues from diffusion weighted images (DWIs). These include Diffusion Imaging (DI), Diffusion Tensor Imaging (DTI), isotropically weighted imaging, and q-space imaging. Just as DI ...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.1940080707
更新日期:1995-11-01 00:00:00
abstract::Dynamic contrast-enhanced (DCE) magnetic resonance imaging (MRI) and proton (1H) magnetic resonance spectroscopy (MRS) provide structural and biochemical information, including vascular volume, vascular permeability and tissue metabolism. In this study, we performed analysis of the enhancement characteristic from DCE-...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1511
更新日期:2010-10-01 00:00:00
abstract::By quantification of brain metabolites, localized brain proton MRS can non-invasively provide biochemical information from distinct regions of the brain. Quantification of short-TE signals is usually based on a metabolite basis set. The basis set can be obtained by two approaches: (1) by measuring the signals of metab...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1234
更新日期:2008-07-01 00:00:00