Labeling of cells with ferumoxides-protamine sulfate complexes does not inhibit function or differentiation capacity of hematopoietic or mesenchymal stem cells.

Abstract:

:Two FDA-approved agents, ferumoxides (Feridex), a suspension of superparamagnetic iron oxide (SPIO) nanoparticles and protamine sulfate, a drug used to reverse heparin anticoagulation, can be complexed and used to label cells magnetically ex vivo. Labeling stem cells with ferumoxides-protamine sulfate (FePro) complexes allows for non-invasive monitoring by MRI. However, in order for stem cell trials or therapies to be effective, this labeling technique must not inhibit the ability of cells to differentiate. In this study, we examined the effect of FePro labeling on stem cell differentiation. Viability, phenotypic expression and differential capacity of FePro labeled CD34 + hematopoietic stem cells (HSC) and mesenchymal stem cells (MSC) were compared with unlabeled control cells. Colony-forming unit (CFU) assays showed that the capacity to differentiate was equivalent for labeled and unlabeled HSC. Furthermore, labeling did not alter expression of surface phenotypic markers (CD34, CD31, CXCR4, CD20, CD3 and CD14) on HSC, as measured by flow cytometry. SDF-1-induced HSC migration and HSC differentiation to dendritic cells were also unaffected by FePro labeling. Both FePro-labeled and unlabeled MSC were cultured in chondrogenesis-inducing conditions. Alcian blue staining for proteoglycans revealed similar chondrogenic differentiation for both FePro-labeled and unlabeled cells. Furthermore, collagen X proteins, indicators of cartilage formation, were detected at similar levels in both labeled and unlabeled cell pellets. Prussian blue staining confirmed that cells in labeled pellets contained iron oxide, whereas cells in unlabeled pellets did not. It is concluded that FePro labeling does not alter the function or differentiation capacity of HSC and MSC. These data increase confidence that MRI studies of FePro-labeled HSC or MSC will provide an accurate representation of in vivo trafficking of unlabeled cells.

journal_name

NMR Biomed

journal_title

NMR in biomedicine

authors

Arbab AS,Yocum GT,Rad AM,Khakoo AY,Fellowes V,Read EJ,Frank JA

doi

10.1002/nbm.991

subject

Has Abstract

pub_date

2005-12-01 00:00:00

pages

553-9

issue

8

eissn

0952-3480

issn

1099-1492

journal_volume

18

pub_type

杂志文章
  • Simultaneous assessment of both lung morphometry and gas exchange function within a single breath-hold by hyperpolarized 129 Xe MRI.

    abstract::During the measurement of hyperpolarized 129 Xe magnetic resonance imaging (MRI), the diffusion-weighted imaging (DWI) technique provides valuable information for the assessment of lung morphometry at the alveolar level, whereas the chemical shift saturation recovery (CSSR) technique can evaluate the gas exchange func...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/nbm.3730

    authors: Zhong J,Zhang H,Ruan W,Xie J,Li H,Deng H,Han Y,Sun X,Ye C,Zhou X

    更新日期:2017-08-01 00:00:00

  • Characterization of a rat orthotopic pancreatic head tumor model using three-dimensional and quantitative multi-parametric MRI.

    abstract::The purpose of this study was to investigate the reliability of 3D isotropic MRI and quantitative multi-parametric MRI characterization on an orthotopic pancreatic head tumor model in rats. 3D isotropic T2 -weighted MRI was performed as a routine for tumor longitudinal follow-up and volume estimation. Common bile duct...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/nbm.3676

    authors: Yin T,Peeters R,Feng Y,Liu Y,Yu J,Dymarkowski S,Himmelreich U,Oyen R,Ni Y

    更新日期:2017-02-01 00:00:00

  • Regional neurochemical profiles in the human brain measured by ¹H MRS at 7 T using local B₁ shimming.

    abstract::Increased sensitivity and chemical shift dispersion at ultra-high magnetic fields enable the precise quantification of an extended range of brain metabolites from (1)H MRS. However, all previous neurochemical profiling studies using single-voxel MRS at 7 T have been limited to data acquired from the occipital lobe wit...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/nbm.1727

    authors: Emir UE,Auerbach EJ,Van De Moortele PF,Marjańska M,Uğurbil K,Terpstra M,Tkáč I,Oz G

    更新日期:2012-01-01 00:00:00

  • Quantitation by (1)H-NMR of dolichol, cholesterol and choline-containing lipids in extracts of normal and phathological thyroid tissue.

    abstract::Proton magnetic resonance spectroscopy at 1.9 T was used to quantify dolichols, cholesterols, choline-containing phospholipids and double bonds in unsaturated acyl chains in lipid extracts of four types of thyroid tissue [normal (n = 27), papillary cancer (n = 15), adenoma (n = 13) and Basedow disease (n = 6)]. In nor...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/1099-1492(200011)13:7<377::aid-nbm658>3.0.

    authors: Yoshioka Y,Sasaki J,Yamamoto M,Saitoh K,Nakaya S,Kubokawa M

    更新日期:2000-11-01 00:00:00

  • Cluster analysis of DCE-MRI data identifies regional tracer-kinetic changes after tumor treatment with high intensity focused ultrasound.

    abstract::Evaluation of high intensity focused ultrasound (HIFU) treatment with MRI is generally based on assessment of the non-perfused volume from contrast-enhanced T1-weighted images. However, the vascular status of tissue surrounding the non-perfused volume has not been extensively investigated with MRI. In this study, clus...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/nbm.3406

    authors: Jacobs I,Hectors SJ,Schabel MC,Grüll H,Strijkers GJ,Nicolay K

    更新日期:2015-11-01 00:00:00

  • Assessing manganese efflux using SEA0400 and cardiac T1-mapping manganese-enhanced MRI in a murine model.

    abstract::The sodium-calcium exchanger (NCX) is one of the transporters contributing to the control of intracellular calcium (Ca(2+)) concentration by normally mediating net Ca(2+) efflux. However, the reverse mode of the NCX can cause intracellular Ca(2+) concentration overload, which exacerbates the myocardial tissue injury r...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/nbm.1414

    authors: Waghorn B,Yang Y,Baba A,Matsuda T,Schumacher A,Yanasak N,Hu TC

    更新日期:2009-10-01 00:00:00

  • A statistical clustering approach to discriminating perfusion from conduit vessel signal contributions in a pulmonary ASL MR image.

    abstract::The measurement of pulmonary perfusion (blood delivered to the capillary bed within a voxel) using arterial spin labeling (ASL) magnetic resonance imaging is often complicated by signal artifacts from conduit vessels that carry blood destined for voxels at a distant location in the lung. One approach to dealing with c...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/nbm.3358

    authors: Walker SC,Asadi AK,Hopkins SR,Buxton RB,Prisk GK

    更新日期:2015-09-01 00:00:00

  • Single echo acquisition MRI using RF encoding.

    abstract::Encoding of spatial information in magnetic resonance imaging is conventionally accomplished by using magnetic field gradients. During gradient encoding, the position in k-space is determined by a time-integral of the gradient field, resulting in a limitation in imaging speed due to either gradient power or secondary ...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/nbm.1399

    authors: Wright SM,McDougall MP

    更新日期:2009-11-01 00:00:00

  • Assessment of cortical damage in early multiple sclerosis with quantitative T2 relaxometry.

    abstract::T2 relaxation time is a quantitative MRI in vivo surrogate of cerebral tissue damage in multiple sclerosis (MS) patients. Cortical T2 prolongation is a known feature in later disease stages, but has not been demonstrated in the cortical normal appearing gray matter (NAGM) in early MS. This study centers on the quantit...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/nbm.3486

    authors: Gracien RM,Reitz SC,Hof SM,Fleischer V,Zimmermann H,Droby A,Steinmetz H,Zipp F,Deichmann R,Klein JC

    更新日期:2016-04-01 00:00:00

  • High spatial resolution MRI and proton MRS of human frontal cortex.

    abstract::High-resolution MR imaging (312 microns in plane resolution) and MR spectroscopy (0.36 cm3 nominal voxel) have been performed on human frontal cortex using a 3 in surface coil. Localized proton spectra have been obtained from contiguous 6 x 6 x 10 mm voxels using one-dimensional phase encoding, TR 2000 ms and TE 31 ms...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/(SICI)1099-1492(199610)9:7<297::AID-NBM433

    authors: López-Villegas D,Kimura H,Tunlayadechanont S,Lenkinski RE

    更新日期:1996-10-01 00:00:00

  • Manganese-enhanced magnetic resonance imaging (MEMRI) of mouse brain development.

    abstract::Given the importance of genetically modified mice in studies of mammalian brain development and human congenital brain diseases, MRI has the potential to provide an efficient in vivo approach for analyzing mutant phenotypes in the early postnatal mouse brain. The combination of reduced tissue contrast at the high magn...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/nbm.932

    authors: Wadghiri YZ,Blind JA,Duan X,Moreno C,Yu X,Joyner AL,Turnbull DH

    更新日期:2004-12-01 00:00:00

  • 1 H NMRS of carnosine combined with 31 P NMRS to better characterize skeletal muscle pH dysregulation in Duchenne muscular dystrophy.

    abstract::In recent years, quantitative nuclear magnetic resonance imaging and spectroscopy (NMRI and NMRS) have been used more systematically as outcome measures in natural history and clinical trial studies for Duchenne muscular dystrophy (DMD). Whereas most of these studies have emphasized the evaluation of the fat fraction ...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/nbm.3839

    authors: Reyngoudt H,Turk S,Carlier PG

    更新日期:2018-01-01 00:00:00

  • Continuous noninvasive monitoring of transcutaneous blood gases for a stable and persistent BOLD contrast in fMRI studies in the rat.

    abstract::The physiological status of anesthetized rats greatly influences blood oxygenation level dependent (BOLD) functional magnetic resonance imaging (fMRI). Monitoring of physiological parameters, particularly partial pressure of carbon dioxide (pCO(2)) levels in the blood, is therefore an important part in the design and ...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/nbm.978

    authors: Ramos-Cabrer P,Weber R,Wiedermann D,Hoehn M

    更新日期:2005-11-01 00:00:00

  • Phosphorus MR spectroscopy in the treatment of human extremity sarcomas.

    abstract::The application of 31P MR spectroscopy in the characterization and treatment of malignant human extremity tumors is reviewed and placed in the perspective of results obtained in murine sarcomas. Despite the now widespread acquisition of gradient localized spectral maps, the low spatial resolution that can be achieved ...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章,评审

    doi:10.1002/(sici)1099-1492(1998110)11:7<341::aid-nbm5

    authors: Sijens PE

    更新日期:1998-11-01 00:00:00

  • Cardiac lipid levels show diurnal changes and long-term variations in healthy human subjects.

    abstract::(1) H-MRS is regularly applied to determine lipid content in ectopic tissue - mostly skeletal muscle and liver - to investigate physiological and/or pathologic conditions, e.g. insulin resistance. Technical developments also allow non-invasive in vivo assessment of cardiac lipids; however, basic data about methodologi...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/nbm.3186

    authors: Ith M,Stettler C,Xu J,Boesch C,Kreis R

    更新日期:2014-11-01 00:00:00

  • Detection of bioradicals by in vivo L-band electron spin resonance spectrometry.

    abstract::The applications of in vivo electron paramagnetic resonance (EPR) or electron spin resonance (ESR) spectroscopy have been impressive over a relatively short period despite the many obstacles which had to be overcome, such as dielectric absorption and biodestruction. The loop-gap resonators and modified loop coil syste...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章,评审

    doi:10.1002/nbm.898

    authors: Fujii H,Berliner LJ

    更新日期:2004-08-01 00:00:00

  • Leakage of metabolites from tissue biopsies can result in large errors in quantitation by MRS.

    abstract::The leakage of metabolites from frozen and thawed tissue biopsies was measured semi-quantitatively by high-field (8.5 T) proton MRS. Human prostate and rat brain tissue specimens, frozen within 1 min of collection, lost significant and variable amounts of diagnostic metabolites immediately upon thawing. For prostate t...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/nbm.815

    authors: Bourne R,Dzendrowskyj T,Mountford C

    更新日期:2003-04-01 00:00:00

  • Apparent diffusion coefficient of the prostate in men prior to biopsy: determination of a cut-off value to predict malignancy of the peripheral zone.

    abstract::Determination of the apparent diffusion coefficient (ADC) of the prostate in men (n = 60) with raised prostate-specific antigen (PSA) or an abnormal digital rectal examination (DRE) prior to transrectal ultrasound (TRUS) guided biopsy using endorectal DWI is reported. Patients were categorized into different groups ba...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/nbm.1114

    authors: Kumar V,Jagannathan NR,Kumar R,Thulkar S,Gupta SD,Dwivedi SN,Hemal AK,Gupta NP

    更新日期:2007-08-01 00:00:00

  • In vivo detection and automatic analysis of GABA in the mouse brain with MEGA-PRESS at 9.4 T.

    abstract::The goals of this study were to develop an acquisition protocol and the analysis tools for Meshcher-Garwood point-resolved spectroscopy (MEGA-PRESS) in mouse brain at 9.4 T, to allow the in vivo detection of γ-aminobutyric acid (GABA) and to examine whether isoflurane alters GABA levels in the thalamus during anesthes...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/nbm.3837

    authors: Guo J,Gang Z,Sun Y,Laine A,Small SA,Rothman DL

    更新日期:2018-01-01 00:00:00

  • 13C NMR spectroscopic analysis of phospholipid metabolism in adrenal chromaffin cells.

    abstract::Phosphatidylcholine (PC) metabolism in bovine adrenal chromaffin cells has been studied in vivo with 100 MHz 13C NMR spectroscopy. The incorporation of 13[CH3]choline was examined in cells embedded in 1% agarose strands and perfused at 37 degrees C. The pattern of 13C NMR spectroscopy. The incorporation of [13CH3]chol...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/nbm.1940040304

    authors: Rivera CJ,Plishker GA,Morrisett JD,Moore J,Percy AK

    更新日期:1991-06-01 00:00:00

  • Lung ventilation volumetry with same-breath acquisition of hyperpolarized gas and proton MRI.

    abstract::The purpose of this work was to assess the reproducibility of percentage of ventilated lung volume (PV) measured from hyperpolarized (HP) (3)He and (1)H anatomical images acquired in the same breath-hold when compared with PV measured from (3)He and (1)H images from separate breath-holds. Volumetric (3)He ventilation ...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/nbm.3187

    authors: Horn FC,Tahir BA,Stewart NJ,Collier GJ,Norquay G,Leung G,Ireland RH,Parra-Robles J,Marshall H,Wild JM

    更新日期:2014-12-01 00:00:00

  • Investigating the regional effect of the chemical shift displacement artefact on the J-modulated lactate signal at ultra high-field.

    abstract::The present work aims to show the applicability of an analytical model for the optimisation of the STEAM sequence timing parameters for lactate detection at ultra high-field. The effects of the chemical shift displacement artefact on the J-modulated signal for a weakly-coupled spin system were considered in the three ...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/nbm.4440

    authors: Fernandes CC,Lanz B,Chen C,Morris PG,Salmon CG

    更新日期:2020-11-02 00:00:00

  • Myocardial T1 mapping using SMART1 Map and MOLLI mapping in asymptomatic patients with recent extracardiac sarcoidosis.

    abstract:INTRODUCTION:Sarcoidosis is a systemic granulomatous disease affecting in particular the respiratory tract. Cardiac magnetic resonance (CMR), including a measurement of T1 relaxation time, could potentially detect early stadia of sarcoidosis of the heart. The study aims to assess T1 mapping in the detection of early ca...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/nbm.4388

    authors: Panovský R,Doubková M,Holeček T,Máchal J,Feitová V,Masárová L,Opatřil L,Mojica-Pisciotti ML,Kincl V

    更新日期:2020-11-01 00:00:00

  • Electro-optic probe for real-time assessments of RF electric field produced in an MRI scanner: Feasibility tests at 3 and 4.7 T.

    abstract::During magnetic resonance imaging (MRI) examinations, the average specific absorption rate (SAR) of the whole body is calculated as an index of global energy deposition in biological tissue without taking into account the presence of metallic implants or conductive materials. However, this global SAR calculation is no...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/nbm.3849

    authors: Saniour I,Gaborit G,Perrier AL,Gillette L,Revillod G,Sablong R,Duvillaret L,Beuf O

    更新日期:2018-01-01 00:00:00

  • Absolute quantification of metabolites in breast cancer cell extracts by quantitative 2D (1) H INADEQUATE NMR.

    abstract::Metabolomic studies by NMR spectroscopy are increasingly employed for a variety of biomedical applications. A very standardized 1D proton NMR protocol is generally employed for data acquisition, associated with multivariate statistical tests. Even if targeted approaches have been proposed to quantify metabolites from ...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/nbm.1816

    authors: Martineau E,Tea I,Akoka S,Giraudeau P

    更新日期:2012-08-01 00:00:00

  • An in vivo ultrahigh field 14.1 T (1) H-MRS study on 6-OHDA and α-synuclein-based rat models of Parkinson's disease: GABA as an early disease marker.

    abstract::The detection of Parkinson's disease (PD) in its preclinical stages prior to outright neurodegeneration is essential to the development of neuroprotective therapies and could reduce the number of misdiagnosed patients. However, early diagnosis is currently hampered by lack of reliable biomarkers. (1) H magnetic resona...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章

    doi:10.1002/nbm.2817

    authors: Coune PG,Craveiro M,Gaugler MN,Mlynárik V,Schneider BL,Aebischer P,Gruetter R

    更新日期:2013-01-01 00:00:00

  • MRI in mouse developmental biology.

    abstract::Mice are used in many studies to determine the role of genetic and molecular factors in mammalian development and human congenital diseases. MRI has emerged as a major method for analyzing mutant and transgenic phenotypes in developing mice, at both embryonic and neonatal stages. Progress in this area is reviewed, wit...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章,评审

    doi:10.1002/nbm.1146

    authors: Turnbull DH,Mori S

    更新日期:2007-05-01 00:00:00

  • Electric properties tomography: Biochemical, physical and technical background, evaluation and clinical applications.

    abstract::Electric properties tomography (EPT) derives the patient's electric properties, i.e. conductivity and permittivity, using standard magnetic resonance (MR) systems and standard MR sequences. Thus, EPT does not apply externally mounted electrodes, currents or radiofrequency (RF) probes, as is the case in competing techn...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章,评审

    doi:10.1002/nbm.3729

    authors: Katscher U,van den Berg CAT

    更新日期:2017-08-01 00:00:00

  • In vivo characterization of fatty acids in human adipose tissue using natural abundance 1H decoupled 13C MRS at 1.5 T: clinical applications to dietary therapy.

    abstract::Natural abundance proton-decoupled (13)C magnetic resonance spectroscopy was used to establish the in vivo lipid composition of normal adipose tissue and the corresponding effects of altered lipid diets. Experiments were performed on a standard 1.5 T clinical MR scanner using a double-tuned (1)H-(13)C coil. Peaks from...

    journal_title:NMR in biomedicine

    pub_type: 临床试验,杂志文章

    doi:10.1002/nbm.824

    authors: Hwang JH,Bluml S,Leaf A,Ross BD

    更新日期:2003-05-01 00:00:00

  • Multi-center reproducibility of neurochemical profiles in the human brain at 7 T.

    abstract::The purpose of this work was to harmonize data acquisition and post-processing of single voxel proton MRS ((1) H-MRS) at 7 T, and to determine metabolite concentrations and the accuracy and reproducibility of metabolite levels in the adult human brain. This study was performed in compliance with local institutional hu...

    journal_title:NMR in biomedicine

    pub_type: 杂志文章,多中心研究

    doi:10.1002/nbm.3252

    authors: van de Bank BL,Emir UE,Boer VO,van Asten JJ,Maas MC,Wijnen JP,Kan HE,Oz G,Klomp DW,Scheenen TW

    更新日期:2015-03-01 00:00:00