Abstract：
:We developed a detailed mathematical model describing the coupling between the molecular weight distribution dynamics of poly(3-hydroxybutyrate-co-3hydroxyvalerate) (PHBV) copolymer chains with those of hydroxybutyrate (HB) and hydroxyvalerate (HV) monomer formation. Sensitivity analysis of the model revealed that both the monomer composition and the molecular weight distribution of the copolymer chains are strongly affected by the ratio between the rates at which the two-monomer units are incorporated into the chains. This ratio depends on the relative HB and HV availability, which in turn is a function of the expression levels of genes encoding enzymes that catalyze monomer formation. Regulation of gene expression was accomplished through the aid of an artificial genetic network, the patterns of expression of which can be controlled by appropriately tuning the concentration of an extracellular inducer. Extensive simulations were used to study the effects of operating conditions and parameter uncertainties on the range of achievable copolymer compositions. Since the predicted conditions fell in the range of feasible bioprocessing manipulations, it is expected that such strategy could be successfully employed. Thus, the presented model constitutes a powerful tool for designing genetic networks that can drive the formation of PHBV copolymer structures with desirable characteristics.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Iadevaia S,Mantzaris NVdoi
10.1016/j.jbiotec.2005.08.030subject
Has Abstractpub_date
2006-03-09 00:00:00pages
99-121issue
1eissn
0168-1656issn
1873-4863pii
S0168-1656(05)00542-0journal_volume
122pub_type
杂志文章abstract：:Proteolytically cleaved human 22 kDa growth hormone (22K hGH) between the amino acid residues 134 and 150 by plasmin or other proteases in vitro has been reported to be most active in growth promoting activity. In this study a deleted mutant hGH lacking amino acid residues from 135 to 146 and having more sensitivity t...
journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
pub_type： 杂志文章,评审
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abstract：:The vitamin E family consists of 4 tocopherol and 4 tocotrienol compounds. During recent years, tocotrienols have gained increased interest due to their biological activities that are beyond the vitamin E activity. Here we report the engineering of plasmid-free Escherichia coli strains for an efficient synthesis of 2-...
journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2007.08.017
更新日期：2008-01-20 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/s0168-1656(03)00072-5
更新日期：2003-06-12 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2015.09.024
更新日期：2015-11-20 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/s0168-1656(02)00051-2
更新日期：2002-07-03 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/0168-1656(90)90044-c
更新日期：1990-11-01 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2017.02.021
更新日期：2017-08-20 00:00:00
abstract：:Two extra-cellular endoxylanases (Xyl Ia and Ib) were purified to homogeneity from the newly isolated thermophilic fungus, Myceliophthora sp. IMI 387099. Xyl Ia and Ib, having a molecular mass of approximately 53 kDa and pI of 5.2 and 4.8, respectively, were optimally active at 75 degrees C and at pH 6.0. They were st...
journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2003.12.010
更新日期：2004-04-29 00:00:00
abstract：:A novel bacterial species was isolated from an industrial-scale biogas plant. The isolate Herbinix hemicellulosilytica T3/55(T) is able to degrade crystalline cellulose. Comparative 16S rRNA gene sequencing demonstrated that the isolate is closely related to environmental samples forming a hitherto unknown sub-cluster...
journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2015.07.022
更新日期：2015-11-20 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2006.04.016
更新日期：2006-08-05 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2004.02.009
更新日期：2004-06-10 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2015.07.009
更新日期：2015-10-20 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/0168-1656(90)90021-3
更新日期：1990-04-01 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2018.04.002
更新日期：2018-06-20 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章,评审
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更新日期：2001-09-13 00:00:00
abstract：:The production of cephalosporin C (CPC) and its precursors penicillin N (PEN N), deacetoxycephalosporin C (DAOC) and deacetylcephalosporin C (DAC), with a highly productive strain of Cephalosporin acremonium, was investigated in an 80-1 airlift tower loop reactor with four static mixer modules (Type SMV, Sulzer) (ATLR...
journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/0168-1656(93)90168-m
更新日期：1993-04-01 00:00:00
abstract：:Endo beta-1,4-mannanases (beta-mannanases, EC 3.2.1.78), belonging to CAZy GH5 and GH26 families, catalyze the hydrolysis of structurally different mannans. In this study, the mannanase encoding gene of Aspergillus aculeatus VN was expressed in Aspergillus niger D15#26 using pAN 52-4 vector, under the control of PgpdA...
journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2010.05.012
更新日期：2010-08-02 00:00:00
abstract：:Flavonoids have notable biological activities and have been widely used in the medicinal and chemical industries. However, single-copy integration of heterologous pathway genes limits the production of flavonoids. In this work, we designed and constructed single-step integration of multiple flavonoid (2S)-naringenin b...
journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2020.11.009
更新日期：2021-01-10 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2008.07.1815
更新日期：2008-10-10 00:00:00
abstract：:Glutathione (GSH) is an important bioactive tripeptide and widely used in food, medicine and other industries. Recently, the bifunctional glutathione synthetase, GshF, has been applied for efficient GSH production under inducible promoter. In this study, the constitutive expression of GshF from Streptococcus sanguinis...
journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2018.11.001
更新日期：2019-01-10 00:00:00
abstract：:The biocatalytic efficiency of recombinant Corynebacterium glutamicum expressing the chnB gene encoding cyclohexanone monooxygenase (CHMO) of Acinetobacter calcoaceticus NCIMB 9871 was investigated. Optimization of an expression system and induction conditions enabled the recombinant biocatalyst to produce CHMO to a s...
journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2009.04.008
更新日期：2009-06-15 00:00:00
