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Development of a FIA system with immobilized enzymes for specific post-column detection of purine bases and their nucleosides separated by HPLC column.

Abstract：

:A sensitive and highly selective method for the simultaneous determination of purine bases and their nucleosides is proposed. An amperometric flow-injection system with the two immobilized enzyme reactors (guanase immobilized reactor and purine nucleoside phosphorylase/xanthine oxidase co-immobilized reactor) is used as the specific post-column detection system of HPLC, to convert compounds separated by a reversed-phase. HPLC column to electroactive species (hydrogen peroxide and uric acid) which can be detected at a flow-through platinum electrode. The proposed detection system is specific for a group of purine bases and purine nucleosides and does not respond for purine nucleotides and pyrimidine bases. The linear determination ranges are from 10 pmol to 5 nmol for four purine bases (hypoxanthine, xanthine, guanine, and adenine) and four purine nucleosides (inosine, xanthosine, guanosine, and adenosine). The detection limits are 1.2-5.5 pmol.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Yao T,Matsumoto Y,Wasa T

doi

10.1016/0168-1656(90)90021-3

subject

Has Abstract

pub_date

1990-04-01 00:00:00

pages

89-97

issue

1

eissn

0168-1656

issn

1873-4863

pii

0168-1656(90)90021-3

journal_volume

14

pub_type

杂志文章

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