Abstract:
:Isoprostanes, non-enzymatic peroxidation products of arachidonic acid, are attractive biomarkers of oxidative stress in research in biology, medicine and nutrition. For the appropriate use of biomarkers it is required that these are both biologically and technically valid. Whereas the biological validity of isoprostanes is well-established, it is technically quite complicated to measure isoprostanes and its metabolites in body fluids, and its rapid disappearance from plasma may hamper practical application. This paper shortly introduces isoprostanes as a biomarker for studies with humans, describes a novel fast and sensitive method for measuring isoprostanes in plasma by high-performance liquid chromatography and tandem mass spectrometry, and provides several examples of the use of the method in studies in humans. By taking care of the biological and technical validity of this biomarker it is possible to establish the antioxidant effects of some food ingredients in studies with human volunteers.
journal_name
Amino Acidsjournal_title
Amino acidsauthors
Coolen SA,van Buuren B,Duchateau G,Upritchard J,Verhagen Hdoi
10.1007/s00726-005-0229-2subject
Has Abstractpub_date
2005-12-01 00:00:00pages
429-36issue
4eissn
0939-4451issn
1438-2199journal_volume
29pub_type
杂志文章相关文献
AMINO ACIDS文献大全abstract::Taurine depletion leads to impaired mitochondrial function, as characterized by reduced ATP production and elevated superoxide generation. These defects can fundamentally alter cardiomyocyte function and if left unchanged can result in cell death. To protect against these stresses, cardiomyocytes possess quality contr...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-015-2053-7
更新日期:2015-12-01 00:00:00
abstract::Endurance exercise training as well as leucine supplementation modulates glucose homeostasis and protein turnover in mammals. Here, we analyze whether leucine supplementation alters the effects of endurance exercise on these parameters in healthy mice. Mice were distributed into sedentary (C) and exercise (T) groups. ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-014-1903-z
更新日期:2015-04-01 00:00:00
abstract::Mitochondria are essential cell organelles of eukaryotes. Hence, it is vitally important to develop an automated and reliable method for timely identification of novel mitochondrial proteins. In this study, mitochondrial proteins were encoded by dipeptide composition technology; then, the genetic algorithm-partial lea...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-006-0465-0
更新日期:2007-11-01 00:00:00
abstract::3-Hydroxyaspartic acid and its derivatives are found both in the free form and as peptide constituents in various microorganisms and fungi. Considering the biological importance of this amino acid and its potential utility as a multifunctional building block in organic syntheses, we have developed a short-step, asymme...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-007-0595-z
更新日期:2008-08-01 00:00:00
abstract::Cell-penetrating peptides, a group of short peptides, can traverse cell membranes to enter cells and thus facilitate the uptake of various molecular cargoes. Thus, they have the potential to become powerful drug delivery systems. The correct identification of peptides as cell-penetrating or non-cell-penetrating would ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-015-1974-5
更新日期:2015-07-01 00:00:00
abstract::This work is an extension of our previous work (Hall et al., 1993) on the synthesis and cytotoxic activity of boronated peptides. The aim of this work was to carry out structural modifications of the amine terminal in compounds1 and2, to increase water solubility, and its effect on the cytotoxicity to tumor cell lines...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00806550
更新日期:1995-12-01 00:00:00
abstract::We have developed the first operationally simple and environmentally benign protocol for the aqueous asymmetric Michael addition reaction of chiral nickel(II) glycinate with nitroalkenes. The reactions proceeded smoothly in the presence of TBAB (tetrabutyl ammonium bromide) in neat water at room temperature and provid...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-0870-x
更新日期:2012-05-01 00:00:00
abstract::Protein and peptide oxidation is a key feature in the progression of a variety of disease states and in the poor performance of protein-based products. The present work demonstrates a mass spectrometry-based approach to profiling degradation at the amino acid residue level. Synthetic peptides containing the photosensi...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-009-0440-7
更新日期:2010-06-01 00:00:00
abstract::The aim of this study was to determine the effective and optimum dose of taurine for exercise performance and to maintain tissue taurine concentration. Rats received a respective daily dose of 0, 20, 100, and 500 mg/kg body weight of taurine (EC and ET-1, -2, -3 groups, respectively) for two weeks, and then, were subj...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-004-0133-1
更新日期:2004-12-01 00:00:00
abstract::The enzymes Akt, mTOR, p70(S6K), rpS6, GSK3, and glycogen synthase interact in the control of protein and/or glycogen synthesis in skeletal muscle, and each has been found to respond to exercise and nutrient supplementation. In the present study, we tested the hypothesis that nutrient supplementation post exercise, in...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-007-0620-2
更新日期:2008-06-01 00:00:00
abstract::Anti-tissue transglutaminase (tTG) antibodies are specifically produced in the small-intestinal mucosa of celiac disease (CD) patients. It is now recognized that these antibodies, acting on cell-surface tTG, may play an active role in CD pathogenesis triggering an intracellular response via the activation of different...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1120-y
更新日期:2013-01-01 00:00:00
abstract::The translation elongation factor eIF5A is conserved through evolution and is necessary to rescue the ribosome during translation elongation of polyproline-containing proteins. Although the site of eIF5A binding to the ribosome is known, no systematic analysis has been performed so far to determine the important resid...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-016-2279-z
更新日期:2016-10-01 00:00:00
abstract::This study investigated the effect of different sampling environments on hemolymph amino acid content of individual Drosophila melanogaster larvae. Hemolymph was collected from individual third instar larvae under cold-anesthetized, awake, and stress conditions. Qualitative and quantitative hemolymph amino acid analys...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-009-0284-1
更新日期:2010-03-01 00:00:00
abstract::Placental vascular formation and blood flow are crucial for fetal survival, growth and development, and arginine regulates vascular development and function. This study determined the effects of dietary arginine or N-carbamylglutamate (NCG) supplementation during late gestation of sows on the microRNAs, vascular endot...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-0948-5
更新日期:2012-06-01 00:00:00
abstract::Developmental changes in the levels of the excitatory amino acids L-glutamate (Glu) and L-Aspartate (Asp) and inhibitory amino acids glycine (Gly) and gamma-amino butyric acid (GABA), as well as taurine and its related amino acids L-methionine (Met), L-cysteine (Cys) and L-serine (Ser) in the brain and pectoralis musc...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-008-0068-z
更新日期:2009-02-01 00:00:00
abstract::The hepatitis B core (HBc) protein has been used successfully in numerous experiments as a carrier for heterologous peptides. Folding and capsid formation of the chimeric proteins is not always achieved easily. In silico analyses were performed to provide further comprehension of the feasibility for predicting success...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-009-0365-1
更新日期:2010-05-01 00:00:00
abstract::This paper analyses the presence of and changes in free amino acids and biogenic amines in red and white muscle of bigeye tuna during storage in controlled atmospheres with 2 gas mixes containing different concentrations of CO(2) and O(2). Levels of amines were generally higher in white than in red muscle, with the ex...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-003-0054-4
更新日期:2004-03-01 00:00:00
abstract::We studied in vivo production of taurine, hypotaurine and sulfate following subcutaneous administration of L-cysteinesulfinate (CSA) to rats and mice. When 5.0 mmol/kg of body weight of CSA was injected to rats, increased urinary excretions of taurine, hypotaurine and sulfate in 24 h urine were 617, 52 and 1,767 micro...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-005-0277-7
更新日期:2006-07-01 00:00:00
abstract::We describe six children with tetrahydrobiopterin (BH(4)) responsive phenylalanine hydroxylase (PAH) deficiency. All patients carry two mutant alleles in the PAH gene. Cofactor deficiency was excluded. The effect of BH(4) administration was studied by correlating different oral BH(4) doses with plasma phenylalanine le...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-002-0354-0
更新日期:2003-07-01 00:00:00
abstract::Alterations in adipocyte characteristics are highly implicated in the pathology of obesity. In a recent article, we demonstrated that high-fat diet-induced obesity impairs lysosomal function, thereby suppressing autophagy in mice white adipose tissue. Taurine, an amino acid naturally contained in the normal diet and e...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-018-2550-6
更新日期:2018-05-01 00:00:00
abstract::This study investigated the effect of taurine and β-alanine supplementation on muscle function and muscle taurine transporter (TauT) protein expression in mdx mice. Wild-type (WT) and mdx mice (5 months) were supplemented with taurine or β-alanine for 4 weeks, after which in vitro contractile properties, fatigue resis...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-016-2292-2
更新日期:2016-11-01 00:00:00
abstract::The daily requirement of a 70-kg male for creatine is about 2 g; up to half of this may be obtained from a typical omnivorous diet, with the remainder being synthesized in the body Creatine is a carninutrient, which means that it is only available to adults via animal foodstuffs, principally skeletal muscle, or via su...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-016-2188-1
更新日期:2016-08-01 00:00:00
abstract::Muscle carnosine synthesis is limited by the availability of beta-alanine. Thirteen male subjects were supplemented with beta-alanine (CarnoSyn) for 4 wks, 8 of these for 10 wks. A biopsy of the vastus lateralis was obtained from 6 of the 8 at 0, 4 and 10 wks. Subjects undertook a cycle capacity test to determine tota...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-006-0364-4
更新日期:2007-02-01 00:00:00
abstract::Metformin, the drug of first choice in type 2 diabetes mellitus (T2DM), reduces cardiovascular (CV) morbidity and mortality in part independently of improved glycemic control and changes in traditional risk factors. However, there are discordant reports on the effects of metformin on endothelial function in T2DM. Our ...
journal_title:Amino acids
pub_type: 临床试验,杂志文章
doi:10.1007/s00726-015-1976-3
更新日期:2015-09-01 00:00:00
abstract::The putative translation factor eIF5A is essential for cell viability and is highly conserved from archaebacteria to mammals. This factor is the only cellular protein that undergoes an essential posttranslational modification dependent on the polyamine spermidine, called hypusination. This review focuses on the functi...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-007-0533-0
更新日期:2007-08-01 00:00:00
abstract:OBJECTIVE:Chemo-therapeutic treatment of glioma patients has minor success. Little is known about mechanisms of a pronounced resistance of gliomas towards actual therapies, yet. ABC-1 belongs to the group of transporters known to be involved in the export of hydrophobic substances and vascular regulation. This study in...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-005-0171-3
更新日期:2005-03-01 00:00:00
abstract::S-adenosylmethionine (SAM), a major substrate in 1-C metabolism is a common precursor in the biosynthetic pathways of polyamines and ethylene, two important plant growth regulators, which exhibit opposing developmental effects, especially during fruit ripening. However, the flux of various substrates including SAM int...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-013-1624-8
更新日期:2014-03-01 00:00:00
abstract::Knowing the key features of the structure and the biochemistry of proteins is crucial to improving enzymes of industrial interest like β-fructofuranosidase. Gene sacA from Bacillus licheniformis ATCC 14580 codifies a sucrose-6-phosphate hydrolase, a β-fructofuranosidase (E.C. 3.1.2.26, protein BlsacA), which has no cr...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-018-02690-2
更新日期:2019-04-01 00:00:00
abstract::It is controversial whether homocysteic acid or other homocysteine derivatives show growth promoting effects. In a clonogenic assay we could show that homocysteine thiolactone and its alpha alkylated derivative increased colony formation significantly. Our work favorizes previous observations showing growth promoting ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00808453
更新日期:1994-02-01 00:00:00
abstract::Spermine oxidase (SMO) was discovered much more recently than other enzymes involved in polyamine metabolism; this review summarizes 10 years of researches on this enzyme. Spermine oxidase (SMO) is a FAD-dependent enzyme that specifically oxidizes spermine (Spm) and plays a dominant role in the highly regulated mammal...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-011-1014-z
更新日期:2012-02-01 00:00:00