Abstract:
:Microvessels are composed of endothelial cells that cover the luminal surface and pericytes that wrap around and along endothelial cells. In the present study, we investigated the regulation of fibrinolysis in cultured human brain microvascular pericytes and endothelial cells after exposure to thrombin. It was found that the regulation in pericytes is different from that in endothelial cells. Specifically, thrombin increases the secretion of fibrinolytic proteins (tissue- and urokinase-type plasminogen activators and plasminogen activator inhibitor-1), resulting in an enhancement of plasminogen activator activity in endothelial cells, whereas the proteinase increases the secretion of only plasminogen activator inhibitor-1 by activation of proteinase-activated receptor-1 and induces suppression of plasminogen activator activity in pericytes. The present data suggest that thrombin regulation of fibrinolytic activity in endothelial cells and pericytes may be involved in the removal of intravascular thrombi and the maintenance of hemostasis, respectively, in human brain microvessels.
journal_name
Biol Pharm Bulljournal_title
Biological & pharmaceutical bulletinauthors
Yamamoto C,Sugato M,Fujiwara Y,Kaji Tdoi
10.1248/bpb.28.208subject
Has Abstractpub_date
2005-02-01 00:00:00pages
208-11issue
2eissn
0918-6158issn
1347-5215pii
JST.JSTAGE/bpb/28.208journal_volume
28pub_type
杂志文章abstract::Reactive oxygen species (ROS) are produced in various cells and affect many biological processes. We previously reported that 2-methyl-1,4-naphtoquinone (menadione) inhibited Ca(2+) influx from the extracellular medium and exocytosis evoked by antigen stimulation in the mast cell line, RBL-2H3. Mast cells release vari...
journal_title:Biological & pharmaceutical bulletin
pub_type: 杂志文章
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journal_title:Biological & pharmaceutical bulletin
pub_type: 杂志文章
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更新日期:2005-10-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
pub_type: 杂志文章
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更新日期:2014-01-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
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更新日期:2014-01-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
pub_type: 杂志文章
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journal_title:Biological & pharmaceutical bulletin
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doi:10.1248/bpb.b13-00738
更新日期:2014-01-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1998-12-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
pub_type: 杂志文章
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更新日期:1995-09-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
pub_type: 杂志文章
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更新日期:2020-10-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
pub_type: 杂志文章
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更新日期:1996-06-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
pub_type: 杂志文章
doi:10.1248/bpb.18.1694
更新日期:1995-12-01 00:00:00
abstract::Fine particulate matter (PM2.5) pollution causes serious health disorders, because PM2.5 becomes deposited in the tracheobronchial and alveoli regions. In the extrathoracic region, there are more deposits of coarse particulate matter than fine particulates. As adverse health issues caused by coarse particulates have n...
journal_title:Biological & pharmaceutical bulletin
pub_type: 杂志文章
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更新日期:2021-01-01 00:00:00
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更新日期:2007-03-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
pub_type: 杂志文章
doi:10.1248/bpb.21.784
更新日期:1998-07-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
pub_type: 杂志文章
doi:10.1248/bpb.18.1750
更新日期:1995-12-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
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journal_title:Biological & pharmaceutical bulletin
pub_type: 杂志文章
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更新日期:2017-01-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
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更新日期:2009-12-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
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更新日期:2019-01-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
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更新日期:2009-04-01 00:00:00
abstract::Mouse brain microsomes contain an amidase that catalyzes the hydrolysis of N-arachidonylethanolamide to arachidonic acid and ethanolamine. The enzymatic activity is dependent on the protein concentration of the microsomes and observed over a wide range of pH, 7.4 to 9.5. Kinetic analysis indicated that K(m) (microM) a...
journal_title:Biological & pharmaceutical bulletin
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更新日期:1996-08-01 00:00:00
abstract::This paper deals with the effects of elenoside, (3-hidroxymethyl-1-methoxy-5,6-methylene-dioxy-4-(3,4-methylenedioxyphenyl)-2-naftoic acid lactone-beta-D-glucoside) an arylnaphthalene lignan with broad spectrum cytotoxicity in a human tumor cell line panel, isolated from Justicia hyssopifolia (Acanthaceae) grown in th...
journal_title:Biological & pharmaceutical bulletin
pub_type: 杂志文章
doi:10.1248/bpb.25.1013
更新日期:2002-08-01 00:00:00
abstract::Follistatin (FS, an activin-binding protein) and activin A (homodimer of inhibin betaA chain) promote and inhibit cell proliferation in rat liver, respectively. The roles of activin AB (heterodimer of inhibin betaA and betaB) and activin B (homodimer of inhibin betaB) in rat liver have not been elucidated yet. In this...
journal_title:Biological & pharmaceutical bulletin
pub_type: 杂志文章
doi:10.1248/bpb.23.755
更新日期:2000-06-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
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更新日期:2015-01-01 00:00:00
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journal_title:Biological & pharmaceutical bulletin
pub_type: 杂志文章
doi:10.1248/bpb.b15-00117
更新日期:2015-01-01 00:00:00
abstract::A rapid and highly sensitive LC-MS-MS method for simultaneous determination of 25-hydroxyvitamin D2 125(OH)D21 and 25-hydroxyvitamin D3 125(OH)D3] in human plasma has been developed using derivatization with a Cookson-type reagent, 4-12-(6,7-dimethoxy-4-methyl-3-oxo-3,4-dihydroquinoxalyl)ethyll-1,2,4-triazoline-3,5-di...
journal_title:Biological & pharmaceutical bulletin
pub_type: 临床试验,杂志文章
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更新日期:2001-07-01 00:00:00
abstract::Temozolomide (TMZ) is currently the first-line drug used for clinical postoperative or non-surgical chemotherapy for glioma, but acquired and intrinsic resistance to TMZ limits its application. The anti-proliferative effect of formononetin on human glioma cells had been confirmed. To improve therapeutic effects of TMZ...
journal_title:Biological & pharmaceutical bulletin
pub_type: 杂志文章
doi:10.1248/bpb.b18-00002
更新日期:2018-08-01 00:00:00
abstract::This study was performed to examine the effects of the antifibrotic agents TJN-331 and tranilast on mesangial expansion in a rat model of anti-Thy1 nephritis. We first investigated the effects of TJN-331 and tranilast on mesangial expansion induced by anti-Thy1 serum in rats, and determined the counts of glomerular ce...
journal_title:Biological & pharmaceutical bulletin
pub_type: 杂志文章
doi:10.1248/bpb.33.1710
更新日期:2010-01-01 00:00:00