Abstract:
:A safe and effective delivery system with a submicron emulsion for puerarin was studied. Puerarin submicron emulsion was prepared by a novel complex-phase inversion-high press homogenization technology. The mechanism to reduce the hemolysis side effect of puerarin was studied by blood cell counts in rabbits. The average diameter, zeta potential and entrapment efficiency of the emulsion prepared was 198.14+/-8.61 nm, -29.45+/-1.47 mV, 87.32+/-0.34%, respectively. Compared with control group, the red blood cell values, packed cell volume, plasma hemoglobin level, haptoglobin level and osmotic fragility of puerarin i.v. group was significantly different (p<0.05) at 42, 43 d, respectively. The blood cell parameter values of puerarin submicron emulsion group were not significantly different (p>0.05) in contrast to control group. Such observations indicated that the intravascular hemolysis occurred at 42, 43 d in puerarin i.v. group rabbits, the hemolysis did not occur for puerarin emulsion group rabbits. As an explanation for these results, it was proposed that the puerarin was either incorporated into the lipophilic core or intercalated between the phospholipid molecules at the interface. It could be concluded that puerarin submicron emulsions prepared markedly reduced the hemolysis effect of puerarin.
journal_name
Biol Pharm Bulljournal_title
Biological & pharmaceutical bulletinauthors
Yue PF,Hai-Long Yuan HL,Zhu WF,Cong LB,Xie H,Liu ZG,Wang LJ,Xiao XHdoi
10.1248/bpb.31.45subject
Has Abstractpub_date
2008-01-01 00:00:00pages
45-50issue
1eissn
0918-6158issn
1347-5215pii
JST.JSTAGE/bpb/31.45journal_volume
31pub_type
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