Abstract:
:The lactose transport system of Citrobacter freundii was characterized. Both the lactose transport system and beta-galactosidase were induced with either lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG), the latter being the better inducer. The Km values for methyl-beta-D-thiogalactopyranoside (TMG) transport and lactose transport were 0.61 mM and 1.1 mM, respectively, and the Vmax values were 53 nmol/min/mg cell protein and 12 nmol/min/mg cell protein, respectively. Thus, TMG is a better substrate than lactose. Thiogalactopyranoside (TDG) was a very potent competitive inhibitor. Neither Na+ nor Li+ had a significant effect on the TMG transport or the lactose transport. Proton/substrate cotransport (symport) via this system was observed.
journal_name
Biol Pharm Bulljournal_title
Biological & pharmaceutical bulletinauthors
Okazaki N,Tsuda M,Wilson TH,Tsuchiya Tdoi
10.1248/bpb.17.794subject
Has Abstractpub_date
1994-06-01 00:00:00pages
794-7issue
6eissn
0918-6158issn
1347-5215journal_volume
17pub_type
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pub_type: 杂志文章,多中心研究,随机对照试验
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journal_title:Biological & pharmaceutical bulletin
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