Abstract:
:Recent studies have shown that antibodies with low fucose content in their oligosaccharides exhibit highly potent antibody-dependent cellular cytotoxicity (ADCC). However, composites of therapeutic antibodies produced by conventional production systems using cell lines such as Chinese hamster ovary (CHO) and SP2/0 do not necessarily contain sufficient amounts of non-fucosylated antibody species. In this study, we combined two lectin-affinity chromatography techniques, Concanavalin A and Lens culinaris agglutinin, to enrich the non-fucosylated species from therapeutic material using the anti-Her2/neu model antibody. Oligosaccharide analysis by matrix-assisted laser desorption/ionization-time of flight MS following peptide-N-glycosidase F digestion suggested that non-fucosylated antibody could be enriched in the purified fraction with efficient removal of high-mannose species. The ADCC activity of the purified fraction was about 100-fold higher than that of the initial material. The chromatographic strategy presented here can be a useful tool to elevate ADCC activity of antibody materials without concentrating high-mannose oligosaccharides.
journal_name
Biol Pharm Bulljournal_title
Biological & pharmaceutical bulletinauthors
Tojo S,Okazaki A,Wakitani M,Shinkawa T,Uchida K,Suzawa Tdoi
10.1248/bpb.32.1604subject
Has Abstractpub_date
2009-09-01 00:00:00pages
1604-8issue
9eissn
0918-6158issn
1347-5215pii
JST.JSTAGE/bpb/32.1604journal_volume
32pub_type
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