In vivo and in vitro evaluation of essential oils from Ligusticum chuanxiong Hort on the transdermal delivery of flurbiprofen in rabbits.


:The present study was designed to evaluate skin permeation enhancement effect of essential oils from Ligusticum chuanxiong Hort (chuanxiong oil) in rabbits and to compare the in vivo absorption and in vitro permeation using flurbiprofen as a model drug. In vivo results demonstrated that chuanxiong oil showed a rapid and marked permeation enhancement effect. The group with 10% oil exhibited the highest value of area under the curve (AUC) of 418+/-124 microg/ml x h, which was 2.43 times the high of control. The AUC value of 3% oil group (245+/-81.6 microg/ml x h) was similar to that of 5% oleic acid group (235+/-74.5 microg/ml x h). Whereas in vitro results indicated the enhancement of chuanxiong oil was relatively weak. The group with 3% oil appeared to the highest flurbiprofen flux (84.9+/-19.3 microg/cm2/h), to some extent lower than 5% oleic acid group (107+/-5.85 microg/cm2/h). At 10% and 15% concentrations, chuanxiong oil even decreased the flux of flurbiprofen compared with the control. Both in vitro results with pretreated skin and flurbiprofen content accumulated in skin indicated the potential mechanism for the in vitro enhancement of chuanxiong oil was the weakened barrier function by improving in the partitioning of flurbiprofen to the stratum corneum. The discrepancy was noted between the in vivo and in vitro results, indicating only about the weakened barrier function was not enough to explain the sharply increment of in vivo absorption of flurbiprofen by chuanxiong oil. The GS-MS results indicated phthalides identified from chuanxiong oil might mainly contribute to enhance in vivo absorption of flurbiprofen because of its large quantities (91.15%).


Biol Pharm Bull


Zhang LC,Hu JH,Li L,Gao LH,Zhu QG,Li Z,Wang ZZ,Su DF




Has Abstract


2006-06-01 00:00:00














  • Nobiletin suppresses MMP-9 expression through modulation of p38 MAPK activity in human dermal fibrobalsts.

    abstract::We aimed to identify a novel flavonoid from the in-house natural products to suppress matrix metalloproteases (MMPs), which is responsible for degradation of collagen and other extracellular matrix proteins. Total eight natural products were screened for identification of a novel MMP-9 suppressor using MMP-9 reporter ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kim JJ,Korm S,Kim WS,Kim OS,Lee JS,Min HG,Chin YW,Cha HJ

    更新日期:2014-01-01 00:00:00

  • Prevention of Atherosclerosis by the Induction of Microbial Polyamine Production in the Intestinal Lumen.

    abstract::Low molecular weight metabolites produced by the intestinal microbiome that have been associated with health and disease as metabolites need to be constantly absorbed from the intestinal lumen and transported to intestinal epithelial cells and blood. Polyamines, especially spermidine and spermine, are bioactive chemic...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章,评审


    authors: Matsumoto M

    更新日期:2020-01-01 00:00:00

  • Checkpoint kinase 1 is cleaved in a caspase-dependent pathway during genotoxic stress-induced apoptosis.

    abstract::Checkpoint kinase 1 (Chk1) plays important roles in genotoxic stress-induced cell cycle checkpoint and in normal cell cycle progression. Here, we show that Chk1 is cleaved in the treatment of apoptotic dose of etoposide (ETP) or cisplatin (CIS) but not of viable dose in HeLa S3 cells. The cleavage of Chk1 was complete...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Okita N,Kudo Y,Tanuma S

    更新日期:2007-02-01 00:00:00

  • Berberine induces G1 arrest and apoptosis in human glioblastoma T98G cells through mitochondrial/caspases pathway.

    abstract::Berberine is an isoquinoline plant alkaloid with a long history of being used for the treatment of many diseases in Chinese herbal medicine. Berberine has a wide range of biochemical and pharmacological effects, including antitumor activities, but its mechanism of action is not clearly understood. In this study, we in...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Eom KS,Hong JM,Youn MJ,So HS,Park R,Kim JM,Kim TY

    更新日期:2008-04-01 00:00:00

  • Diphenyleneiodonium chloride, an inhibitor of reduced nicotinamide adenine dinucleotide phosphate oxidase, suppresses light-dependent induction of clock and DNA repair genes in zebrafish.

    abstract::In most species, solar light is both a DNA-damaging agent and the key entraining stimulus for the endogenous circadian clock. The zebrafish is an attractive vertebrate system in which to study the influence of light on gene expression because the DNA repair proteins and circadian oscillators in this species are light-...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Osaki T,Uchida Y,Hirayama J,Nishina H

    更新日期:2011-01-01 00:00:00

  • Influence of pH on heat-induced aggregation and degradation of therapeutic monoclonal antibodies.

    abstract::Monoclonal antibodies are widely used for the treatment of various diseases, and because therapeutic monoclonal antibodies are stored in an aqueous solution or in a lyophilized state, the preparation of a stabilizing formulation that prevents their deterioration (degradation and aggregation) is crucial. Given the stru...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Ishikawa T,Ito T,Endo R,Nakagawa K,Sawa E,Wakamatsu K

    更新日期:2010-01-01 00:00:00

  • Metabolic fate of ulinastatin (2); Pharmacokinetics in rabbits following intra-articular administration.

    abstract::The absorption and distribution of ulinastatin following intra-articular administration of [125I]ulinastatin to rabbits were determined and kinetic analysis using a multi-compartment model was performed. At 4 h after administration, the content of radioactivity in the synovial fluid, which was comparable to that of th...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Ohzawa N,Takahashi Y,Ogihara T,Nakai Y,Ishiguro J

    更新日期:1997-07-01 00:00:00

  • Studies on palauan medicinal herbs. II. Activation of mouse macrophages RAW 264.7 by Ongael, leaves of Phaleria cumingii (Meisn.) F. Vill. and its acylglucosylsterols.

    abstract::The extract of Ongael [leaves of Phaleria cumingii (MEISN.) F. VILL.], a Palauan medicinal herb, enhanced an in vitro phagocytic activity of mouse macrophages RAW 264.7 cells (RAW 264.7). Activity-guided fractionation of the Ongael extract by the in vitro phagocytosis assay using RAW 264.7 led to the isolation of a mi...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Matsuda H,Tokunaga M,Iwahashi H,Naruto S,Yagi H,Masuko T,Kubo M

    更新日期:2005-05-01 00:00:00

  • Characterization of partially purified alpha-glucosidase in the insoluble fraction of bovine crystalline lens.

    abstract::Two fractions of neutral alpha-glucosidase were partially purified from the insoluble fraction of bovine lens. This is the first report of such an event to the best of our knowledge. The apparent native molecular weights of these fractions were 121 kDa (fraction-I) and 254 kDa (fraction-II). Both fractions contained t...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kamei A,Fujiyama O

    更新日期:1995-08-01 00:00:00

  • Nano-mechanical reinforcement in drug-resistant ovarian cancer cells.

    abstract::The mechanical properties of cells are considered promising biomarkers for the early detection of cancer and the testing of drug efficacy against it. Nevertheless, generalized correlations between drug resistance and the nano-mechanical properties of cancer cells are yet to be defined due to the lack of necessary stud...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Seo YH,Jo YN,Oh YJ,Park S

    更新日期:2015-01-01 00:00:00

  • Region-dependent differences and alterations of protective thiol compound levels in cultured astrocytes and brain tissues.

    abstract::We examined region-dependent differences and alterations in the levels of protective thiol compounds, glutathione (GSH) and metallothionein (MT)-I and -II, in cultured rat astrocytes under several culture conditions and in brain tissues of rats at postnatal and weaning periods. Regardless of culture conditions, both p...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Adachi T,Satoh M,Pramanik R,Kuroda S,Ishido M,Kunimoto M

    更新日期:2006-07-01 00:00:00

  • The effect of zaldaride maleate, an antidiarrheal compound, on acetylcholine-induced intestinal electrolyte secretion.

    abstract::The effect of zaldaride on acetylcholine-induced colonic electrolyte secretion was examined. The short-circuit current response to acetylcholine was partially reduced by tetrodotoxin, a neuronal blocker, and was completely inhibited by atropine, an acetylcholine M receptor antagonist, in the rat colonic preparations. ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Aikawa N,Kishibayashi N,Karasawa A,Ohmori K

    更新日期:2000-11-01 00:00:00

  • Pharmacokinetic drug interactions between ampiroxicam and sulfaphenazole in rats.

    abstract::The aim of the present study was to determine the effect of sulfaphenazole (SP) on the pharmacokinetics of ampiroxicam (AM) which is metabolized by cytochrome P-450 (CYP) 2C9, since SP is a potent inhibitor of CYP 2C9, and so a dramatic pharmacokinetic drug interaction between both drugs is assumed after dosing. Singl...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Ogiso T,Iwaki M,Tanaka H,Kobayashi E,Tanino T,Sawada A,Uno S

    更新日期:1999-02-01 00:00:00

  • Proportionality of protease activities in malignant cells to their metastatic potentials.

    abstract::It has been suggested that the activities of type IV collagenase and/or ectopeptidases possessed by malignant cells are related to their metastatic potentials. In the present study, we examined the activities of three aminopeptidases, two serine proteases, as well as type IV collagenase, in three kinds of cell lines o...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Funahashi T,Shimamura M,Kocha T,Fukuda T,Aoyagi T

    更新日期:1994-08-01 00:00:00

  • Identification of a tryptanthrin metabolite in rat liver microsomes by liquid chromatography/electrospray ionization-tandem mass spectrometry.

    abstract::Tryptanthrin originally isolated from Isatis tinctoria L. has been characterized to have anti-inflammatory activities through the dual inhibition of cyclooxygenase-2 and 5-lipoxygenase mediated prostaglandin and leukotriene syntheses. To characterize phase I metabolite(s), tryptanthrin was incubated with rat liver mic...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Lee SK,Kim GH,Kim DH,Kim DH,Jahng Y,Jeong TC

    更新日期:2007-10-01 00:00:00

  • Cytotoxic activity of bryonolic acid isolated from transformed hairy roots of Trichosanthes kirilowii var. japonica.

    abstract::Bryonolic acid was isolated in high yield from transformed hairy root cultures of Trichosanthes kirilowii var. Japonica. Bryonolic acid exhibited cytotoxic activity to various tumor cells in vitro, independent of cell type. Normal cells such as rat hepatocytes are less sensitive to bryonolic acid. The appearance of a ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kondo T,Inoue M,Mizukami H,Ogihara Y

    更新日期:1995-05-01 00:00:00

  • Pharmacokinetics of granisetron in adults and children with malignant diseases.

    abstract::Granisetron (GRN) is widely used for patients with various cancers who suffer from chemotherapy-induced vomiting and nausea. The, pharmacokinetics of GRN has not been fully evaluated in such patients, however, and its dosage regimen is still controversial. In this study, we determined GRN levels in serum and urine fro...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 临床试验,杂志文章


    authors: Wada I,Takeda T,Sato M,Saitoh H,Nakabayashi T,Mino K,Honma T,Takada M,Hirano K

    更新日期:2001-04-01 00:00:00

  • Studies on thermophile products. X. Further biological properties of isofatty acid-containing phosphatidylglycerol that enhances the induction of suppressor T cells.

    abstract::Isofatty acid-containing phosphatidylglycerol (Fr. 7-C), isolated from Bacillus stearothermophilus UBT8038, enhances the induction of concanavalin A (Con A)-activated suppressor T (Ts) cells in a dose dependent manner (0.01-1 microgram/ml). Its further biological properties on mouse mixed lymphocyte reaction (MLR) has...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kohama Y,Semba T,Tanaka K,Iida K,Moriyama K,Suda T,Tone H,Itoh S,Mimura T

    更新日期:1994-11-01 00:00:00

  • The antiproliferative activity of saponin-enriched fraction from Bupleurum Kaoi is through Fas-dependent apoptotic pathway in human non-small cell lung cancer A549 cells.

    abstract::Bupleuri Radix (Chai-hu in Chinese and Saiko in Japanese) is one of the most important traditional Chinese crude drugs for treating hepatitis malaria and intermittent fever. B. kaoi is one of the Bupleurum spp. families locally found in Taiwan. The effects of saponin-enriched fraction (SEF) from Bupleurum Kaoi in huma...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Hsu YL,Kuo PL,Weng TC,Yen MH,Chiang LC,Lin CC

    更新日期:2004-07-01 00:00:00

  • Osthole reverses beta-amyloid peptide cytotoxicity on neural cells by enhancing cyclic AMP response element-binding protein phosphorylation.

    abstract::Accumulation of β-amyloid peptide (Aβ) in the brain plays an important role in the pathogenesis of Alzheimer’s disease (AD). Previous studies have demonstrated the neuroprotective role of osthole against oxygen and glucose deprivation in cortical neurons. However, the effects of osthole on Aβ-induced neurotoxicity in ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Hu Y,Wen Q,Liang W,Kang T,Ren L,Zhang N,Zhao D,Sun D,Yang J

    更新日期:2013-01-01 00:00:00

  • Onset of clinical effects and plasma concentration of fluvoxamine in Japanese patients.

    abstract::It is widely accepted that selective serotonin reuptake inhibitors (SSRIs) require 2 to 4 weeks of administration before improvements in emotional symptoms of depression are seen. We evaluated whether early monitoring of Hamilton Rating Scale for Depression (HAMD) scores in patients treated with the SSRI fluvoxamine c...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 临床试验,杂志文章


    authors: Katoh Y,Uchida S,Kawai M,Takei N,Mori N,Kawakami J,Kagawa Y,Yamada S,Namiki N,Hashimoto H

    更新日期:2010-01-01 00:00:00

  • Effects of caffeine and theophylline on the development of dental caries in rats.

    abstract::The purpose of this study was to evaluate the influence of caffeine and theophylline on the development of dental caries in rats. Six Wistar dams (spf), mutans streptococci free, were obtained, each with six male pups. The dams were infected by Streptococcus sobrinus 6715 and divided into three groups which received d...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Ruenis AP,Rosalen PL,Volpato MC,Groppo FC

    更新日期:2000-03-01 00:00:00

  • Activation of C-kinase eta through its cholesterol-3-sulfate-dependent phosphorylation by casein kinase I in vitro.

    abstract::The physiological correlation between casein kinase I (CK-I) and an isoform eta of protein kinase C (C-kinase eta) was investigated in vitro, since it has been reported that (i) cholesterol-3-sulfate (CH-3S) effectively activates C-kinase eta rather than the other isoforms (C-kinase epsilon and C-kinase delta) in vitr...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Okano M,Yokoyama T,Miyanaga T,Ohtsuki K

    更新日期:2004-01-01 00:00:00

  • A naturally occurring rexinoid, honokiol, can serve as a regulator of various retinoid x receptor heterodimers.

    abstract::We investigated the properties of honokiol, a natural rexinoid, as a regulator of retinoid X receptor (RXR) heterodimers with various partner nuclear receptors (NRs), in comparison with those of the synthetic rexinoid bexarotene. Honokiol alone was hardly capable of activating peroxisome proliferator-activated recepto...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kotani H,Tanabe H,Mizukami H,Amagaya S,Inoue M

    更新日期:2012-01-01 00:00:00

  • A novel fine granule system for masking bitter taste.

    abstract::In order to prepare fine granules of sparfloxacin (SPFX), a new quinolone anti-bacterial drug that shows masking of the bitter taste of SPFX and dissolutes at a rapid rate, various film-coated fine granules containing 20% SPFX and 0-52% low-substituted hydroxypropylcellulose(L-HPC) in the cores, were prepared by a spr...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Shirai Y,Sogo K,Yamamoto K,Kojima K,Fujioka H,Makita H,Nakamura Y

    更新日期:1993-02-01 00:00:00

  • Comparison of nicotine pharmacokinetics in healthy Japanese male smokers following application of the transdermal nicotine patch and cigarette smoking.

    abstract::Transdermal nicotine patch (TNP) contains approximately 16.6 and 24.9 mg of nicotine per 20 and 30 cm2 (TNP-20 and TNP-30). The aims of the study are to investigate linearity of nicotine pharmacokinetics after single application of different strengths of TNP and to directly compare plasma nicotine concentrations with ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章,随机对照试验


    authors: Sobue S,Sekiguchi K,Kikkawa H,Akasaki M,Irie S

    更新日期:2006-05-01 00:00:00

  • Cooperative Behavior of Fluoroquinolone Combinations against Escherichia coli and Staphylococcus aureus.

    abstract::The effects of different combinations of ciprofloxacin (CIP) and norfloxacin (NOR) against Escherichia coli and Staphylococcus aureus were studied using checkerboard, fractional inhibitory concentration (FIC) and time-kill analysis methods. Results obtained by the checkerboard method showed that the more effectives co...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Vitorino GP,Becerra MC,Barrera GD,Caira MR,Mazzieri MR

    更新日期:2017-01-01 00:00:00

  • P-glycoprotein functions in peripheral-blood CD4+ cells of patients with systemic lupus erythematosus.

    abstract::Over-expression of P-glycoprotein (P-gp) in lymphocytes is implicated in the failure of immunosuppressant therapy. We investigated P-gp function in peripheral-blood CD3+, CD4+, and CD8+ cells of 14 healthy subjects and 12 patients with systemic lupus erythematosus (SLE). P-gp function was estimated by the transporter ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Henmi K,Yoshida M,Yoshikawa N,Hirano T

    更新日期:2008-05-01 00:00:00

  • Glycerol absorption by Na+-dependent carrier-mediated transport in the closed loop of the rat small intestine.

    abstract::The absorption of glycerol was examined using the closed loop of the rat small intestine in situ to clarify the transport mechanism. The absorption of glycerol, evaluated by its disappearance from the intestinal lumen, was saturable and reduced under the Na(+)-free conditions, suggesting the involvement of an Na(+)-de...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kato T,Hayashi Y,Inoue K,Yuasa H

    更新日期:2005-03-01 00:00:00

  • Determination of reduced nicotinamide adenine dinucleotide phosphate concentration using high-performance liquid chromatography with fluorescence detection: ratio of the reduced form as a biomarker of oxidative stress.

    abstract::Reduced nicotinamide adenine dinucleotide phosphate (NADPH) is the principal source of reducing power in numerous processes of physiological importance. We examined the influence of oxidative stress on the relative amounts of NADPH in human red blood cells (RBCs). To determine the homeostasis of the NADPH existing in ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Ogasawara Y,Funakoshi M,Ishii K

    更新日期:2009-11-01 00:00:00