Abstract:
:Serotonin 1A (5HT1A) receptor agonists have shown neuroprotective properties in different models of central nervous system injury. Activation of neuronal 5HT1A receptors appears to be involved in the neuroprotective effects. It remains to be elucidated if astroglial cells are responsive to the 5HT1A neuroprotective effects. The participation of astroglial S100B trophic factor has been proposed since 5HT1A activation leads to S100B release and nanomolar concentration level of this molecule showed pro-survival activity in neuronal cultures. Using the cortical devascularization model (CD; unilateral pial disruption), a procedure that results in localized ischemia without producing direct physical damage to brain tissue, we tested the effects of a full 5HT1A agonist, 8-OH-DPAT, or the antagonist WAY-100635 on cortical neuronal survival, astroglial cell response and S100B expression. Wistar rats were subjected to CD lesion which consisted of a craniotomy followed by physical damage to the underlying pial blood vessels. Two and twenty-four hours after the CD lesion, animals received intraperitoneally 8-OH-DPAT (1 mg/kg), WAY-100635 (1 mg/kg) or vehicle (sterile saline). At 3, 7 or 14 days post-lesion, animals were sacrificed and their brains processed for immunohistochemistry to detect GFAP, vimentin, MAP-2, S100B and nuclear Hoechst staining. S100B level in the brain cortex and serum was quantified by an ELISA assay. Serum S100B was considered an index of S100B release. 8-OH-DPAT treatment reduced neuronal death, dendrite loss, astroglial hypertrophy and hyperplasia. In contrast, WAY-100635 treatment increased these parameters of damage. S100B intracellular immunoreactivity in astrocytes and total S100B level showed long-lasting changes after the CD lesion and subsequent treatments depending on the 5HT1A activity. The level of serum S100B was increased in 8-OH-DPAT-treated animals. Increased damage observed in WAY-100635-treated animals supports the hypothesis that the protective 8-OH-DPAT action may be mediated by specific 5HT1A receptors. The reduction in astroglial hypertrophy and hyperplasia as well as long-term changes in S100B immunoreactivity and increased S100B release that we observed allows us to hypothesize that astroglial cells may play an important role in 5HT1A-mediated neuroprotection.
journal_name
Brain Resjournal_title
Brain researchauthors
Ramos AJ,Rubio MD,Defagot C,Hischberg L,Villar MJ,Brusco Adoi
10.1016/j.brainres.2004.10.019subject
Has Abstractpub_date
2004-12-31 00:00:00pages
201-20issue
2eissn
0006-8993issn
1872-6240pii
S0006-8993(04)01663-4journal_volume
1030pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::We measured the release of neuropeptide Y (NPY) from hippocampal slices of rats at various times after limbic seizures induced by a subcutaneous injection of 12 mg/kg kainic acid (KA). Two days after KA, 100 mM KCl induced a 1.6 +/- 0.2-fold increase in NPY release compared to saline-injected rats (P < 0.05), while sp...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)90847-8
更新日期:1994-10-10 00:00:00
abstract::Retinal and tectal potentials to alternating gratings were recorded in pigeons raised monocularly deprived. Deprived eyes showed consistent myopia and anterior-posterior axis elongation. In addition, vitreal opacities were observed in 70% of the deprived eyes. In pigeons with vitreal opacities, a reduction of retinal ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)90165-9
更新日期:1985-07-15 00:00:00
abstract::In humans and many other mammalian species, the behavioural consequences of a cortical lesion tend to be milder when it occurs early in life, and there is evidence that an important factor contributing to the behavioural sparing in the young is the formation of new thalamo-cortical connections by thalamic neurons init...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2007.05.063
更新日期:2007-09-05 00:00:00
abstract::Diphtheria toxin (DTX) kills cells by inactivating ribosomal translocation and when used to retrogradely intoxicate cat intercostal motoneurones produces marked morphological alterations in Nissl bodies, including those specifically sited postsynaptic to C-type axon terminals. Here, qualitative examinations of 'intoxi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)90302-r
更新日期:1990-02-12 00:00:00
abstract::Previous studies have shown that access to conceptual/semantic information precedes phonological access in alphabetic language production such as English or Dutch. The present study investigated the time course of semantic and orthographic encoding in Chinese (a non-alphabetic language) spoken word production. Partici...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.03.049
更新日期:2009-06-01 00:00:00
abstract::Unilateral injections of 6-hydroxydopamine into the substantia nigra in the rat significantly attenuated cortical spreading depression (CSD)-induced eating from the hemisphere ipsilateral but not contralateral to the lesion. The lateralized decrease in elicited feeding was correlated with postlesion body weight loss, ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(79)90428-1
更新日期:1979-01-12 00:00:00
abstract::Fos expression in the globus pallidus (GP) of rats was elicited by the D2 agonist quinpirole both ipsilateral and contralateral to a unilateral nigrostriatal 6-hydroxydopamine (6-OHDA) injection; however, the 6-OHDA-treated hemisphere was more sensitive to this effect. The quinpirole-induced GP Fos expression was anta...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)91166-p
更新日期:1993-12-31 00:00:00
abstract::The effect of high concentrations of L-ascorbic acid on the in vivo and in vitro growth of human neuroblastoma has been investigated. Directly implemented into cell culture it decreased the DNA, RNA and protein synthesis, and mitosis of neuroblastoma cells, without affecting normal neuronal cells. In vivo treatment of...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)91139-4
更新日期:1985-09-09 00:00:00
abstract::The hypothalamic angiotensin II (Ang II) system plays an important role in pituitary hormone release. Little is known about this system in the mouse brain. We studied the distribution of angiotensin-converting-enzyme (ACE), Ang II, Ang II receptor subtypes, and vasopressin in the hypothalamus of adult male mice. Autor...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)00220-5
更新日期:1997-05-23 00:00:00
abstract::Data are presented which show a different pattern of dopamine and noradrenaline utilization in terminal regions of the A9 and A10 dopaminergic systems and in terminal regions of the dorsal noradrenergic bundle after electrical stimulation of the ventral tegmental area and an adjacent area. At the sites of the electrod...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(83)90505-x
更新日期:1983-06-06 00:00:00
abstract::Commissural and ipsilateral intrinsic connections of the vestibular nuclear complex of cats were investigated using retrograde transport of horseradish peroxidase (HRP). HRP was microiontophoretically injected into limited areas (0.2-0.5 mm in diameter) of the respective vestibular nuclei. In the commissural connectio...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)91474-x
更新日期:1985-08-19 00:00:00
abstract::Hamsters exhibited a circadian variation in metabolic activity determined by [14C]2-deoxyglucose uptake in the suprachiasmatic nuclei, as has been previously reported for rats. The 13-line ground squirrel, a diurnal rodent, did not show a circadian variation in metabolic activity. The data are consistent with the hypo...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(82)90626-6
更新日期:1982-01-28 00:00:00
abstract::The study examined whether intravenous (i.v.) leptin increased Fos-production in spinally projecting neurons in the hypothalamic paraventricular nucleus (PVN). We combined (i) rhodamine tagged microspheres injected into the upper thoracic spinal cord and (ii) Fos (marker of neuronal activation) immunohistochemistry. E...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)01902-2
更新日期:1999-10-09 00:00:00
abstract::The present investigations were designed to assess the effect of the serotoninergic system on luteinizing hormone (LH) and LH-releasing hormone (LH-RH) secretion in female rats aged 14 and 30 days. The administration of 5-hydroxytryptophan (5-HTP; 75 mg/kg i.p.) increased hypothalamic serotonin (5-HT) concentrations i...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)91634-s
更新日期:1990-07-16 00:00:00
abstract::The L-isomer of beta-N-methylamino-L-alanine (BMAA), present in free form in seed of Cycas circinalis, elicits in spinal cord cultures a pattern of acute postsynaptic neuronal vacuolation comparable to that induced by beta-N-oxalylamino-L-alanine (BOAA), an excitotoxic amino acid of greater potency isolated from seed ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)90342-8
更新日期:1987-05-05 00:00:00
abstract::Fast axonal transport and neuronal protein synthesis was studied in the isolated nervous system of Aplysia californica. The abdominal ganglion with attached pleural-abdominal connectives (PAC) was removed and the ganglion pulse-labelled with [35S]methionine for 30 min in vitro. The axon containing connectives were lig...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)90388-9
更新日期:1985-04-15 00:00:00
abstract::Quantal analysis of transmitter release is useful in examining presynaptic mechanisms involved in synaptic transmission. However, in central neurons, the presence of multiple synapses makes it difficult to use the traditional quantal analysis, developed for the neuromuscular transmission. We developed a method to mini...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2008.02.056
更新日期:2008-05-01 00:00:00
abstract::The ventral tegmental area (VTA) is a brain region centrally involved in the development and expression of a variety of behaviors associated with drug use. Orexin/hypocretin (ox/hcrt) and corticotropin releasing factor (CRF) are both peptides released into the VTA, and an increasing number of studies show that both ox...
journal_title:Brain research
pub_type: 杂志文章,评审
doi:10.1016/j.brainres.2009.10.068
更新日期:2010-02-16 00:00:00
abstract::(1) Visual, auditory and olfactory input were not necessary for the performance of the lordosis reflex by female rats. Since taste is not involved in this behavior, somatosensory input must be sufficient for triggering the lordosis reflex. (2) Surgical cutaneous denervation markedly reduced the frequency and strength ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(76)90987-2
更新日期:1976-01-09 00:00:00
abstract::Sodium and potassium currents in the node of Ranvier decrease exponentially with time during long lasting voltage clamp experiments. This decline is strongly dependent on temperature (Q10 approximately 3). Thiamine and, particularly, its diand triphosphoric acid esters are shown to prevent this exponential decline of ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(75)90720-9
更新日期:1975-05-23 00:00:00
abstract::The intermediate, medial part of the hyperstriatum ventrale (IMHV) is an area of the avian forebrain which is essential for two forms of early learning in the domestic chick. We have developed an in vitro slice preparation which contains part of the IMHV and have found that the electrophysiological properties of the a...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(96)00361-7
更新日期:1996-07-15 00:00:00
abstract::Mitochondria critically regulate cytoplasmic Ca(2+) concentration ([Ca(2+)]c), but the effects of sensory neuron injury have not been examined. Using FCCP (1µM) to eliminate mitochondrial Ca(2+) uptake combined with oligomycin (10µM) to prevent ATP depletion, we first identified features of depolarization-induced neur...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2014.09.040
更新日期:2014-11-17 00:00:00
abstract::N-desmethylclozapine (NDMC), a major circulating metabolite of the atypical antipsychotic drug, clozapine, and has M(1) muscarinic receptor partial agonistic property. The purpose of the present study was to examine whether in vivo behavioral effects of NDMC were elicited through the activation of muscarinic receptors...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2011.08.056
更新日期:2011-10-18 00:00:00
abstract::The role of descending monoaminergic pathways in the antinociceptive effect of baclofen following intraperitoneal (i.p.) administration was investigated by determining the effect of intrathecal (i.t.) administration of neurotoxins [6-hydroxydopamine (6-OHDA) and 5,6-dihydroxytryptamine (5,6-DHT)] and receptor antagoni...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)90279-3
更新日期:1985-05-27 00:00:00
abstract::Slowness of goal-directed movements is a frequent symptom following cerebellar lesions. So far it has not been demonstrated whether this slowness represents compensation for impaired braking which is a feature of cerebellar dysfunction with the consequence of hypermetria, or whether it is an independent part of cerebe...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)01514-0
更新日期:1996-03-25 00:00:00
abstract::The blood-brain barrier (BBB) permeability primarily increases in cerebral venules during acute hypertension. Methyl-β-cyclodextrin (MβCD), a cholesterol-depleting agent, decreases the expression of caveolins disrupting caveolar structures. We aimed to determine the effects of MβCD on the BBB permeability of angiotens...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2019.03.024
更新日期:2019-07-15 00:00:00
abstract::Pituitary adenylate cyclase-activating peptide (PACAP) is a 38-amino-acid polypeptide, first isolated from hypothalamus, which directly stimulates in vitro the production of cAMP as well as the release of several pituitary hormones, such as growth hormone and luteinizing hormone. In vivo, PACAP has been shown to stimu...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)01011-1
更新日期:1997-10-31 00:00:00
abstract::Horizontal eye movements, elicited by sinusoidal rotation in darkness, were recorded with a magnetic search coil technique in pigmented rats, hemilabyrinthectomised 8-12 weeks before the investigation. Separate gains during rotation towards the lesioned side (LS) and the intact side (IS) were calculated by a computer ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(98)00329-1
更新日期:1998-06-08 00:00:00
abstract::We have previously found that crocin (crocetin di-gentiobiose ester) antagonizes the inhibitory effect of ethanol on long-term potentiation in the rat hippocampus in vivo and in vitro. To explore mechanisms underlying the antagonism of crocin against ethanol, we investigated the effects of ethanol and crocin on synapt...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)01505-9
更新日期:1998-03-16 00:00:00
abstract::The object of this study was to quantify and compare the degree of control monkeys may assert over firing patterns of normal and epileptic neurons. Thirty-seven epileptic and 70 normal neurons were studied in detail. The operant task was for the monkey to generate specified consecutive interspike intervals (ISI). The ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(78)90884-3
更新日期:1978-08-04 00:00:00