Abstract:
:The establishment of a vascular network within tumours is a key step in the progression towards an aggressive, metastatic state, with poor prognosis. We have developed a novel in vitro model to specifically capture the interaction between endothelial cells and solid tumours. Micro-vascularised in vitro tumour constructs were produced by introducing endothelial cells to multicellular spheroids formed in hanging drops. Upon introduction, the endothelial cells migrated into the tumour spheroid, establishing tubular networks and luminal structures. This system relies on the natural pro-angiogenic capacity of multicellular spheroids, and does not require the addition of exogenous angiogenic factors, or use of extracellular-matrix substitutes.
journal_name
Angiogenesisjournal_title
Angiogenesisauthors
Timmins NE,Dietmair S,Nielsen LKdoi
10.1007/s10456-004-8911-7subject
Has Abstractpub_date
2004-01-01 00:00:00pages
97-103issue
2eissn
0969-6970issn
1573-7209pii
5378911journal_volume
7pub_type
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