[Difficulties with immunofixation interpretation].

abstract：:We investigated two different freezing protocols on mouse embryos that were either grown in vivo or were grown in vitro for a certain period. Our results confirm that an extended in vitro culture period makes the embryo more susceptible for injury due to freezing and thawing. Furthermore when freezing two cell mouse e...

journal_title：Annales de biologie clinique

authors： Van den Abbeel E,Van Steirteghem AC

更新日期：1987-01-01 00:00:00

abstract：:AFP can be measured by radioimmunoassay in 52 hours using an incubation at 18 degrees C for 48 hours and the double antibody solid phase method to separate the free labelled AFP from the labelled AFP bound to the antibodies. In these conditions, the sensitivity and precision of the assay are very satisfactory and the ...

journal_title：Annales de biologie clinique

authors： Franchimont P,Zangerle PF,Debruche ML,Proyard J,Simon M,Gaspard U

更新日期：1975-01-01 00:00:00

abstract：:The recent development of specific and sensitive biochemical markers reflecting the overall rate of bone formation and bone resorption, has markedly improved the non invasive assessment of bone turnover in various metabolic bone diseases, especially for osteoporosis. The immunoassay of human osteocalcin recognizing th...

journal_title：Annales de biologie clinique

authors： Garnero P,Delmas PD

更新日期：1999-03-01 00:00:00

abstract：:Eighteen paraplegics with or without neurogenic osteo-arthropathy (POAN) were evaluated for variations in their urinary excretion levels of total (T), and free (L) hydroxyproline and its non-dialysable (ND) and dialysable (D) peptide fractions. Increases in urinary hydroxyproline, moderate rises in free hydroxyproline...

journal_title：Annales de biologie clinique

authors： Pilonchery G,Finck C,Minaire P,Messy P,Revol A

更新日期：1982-01-01 00:00:00

abstract：:Anemia is the most common pathology encountered in hematology. Etiologies are numerous, so it is important to adopt a rigorous approach. Complementary examinations must be specific to the clinical situation in order to determine the mechanisms on the one hand and decide the therapeutic management on the other. We repo...

journal_title：Annales de biologie clinique

authors： Roche C,Roche NC,Thefenne H,Saidi R,De Pina JJ,Molinier S,Oliver M

更新日期：2011-05-01 00:00:00

abstract：:Premature rupture of the membranes (PROM) is a frequent event affecting 3% of pregnancies. PROM causes 30% of premature deliveries and 20% of perinatal mortality. The diagnosis relies mainly on the clinical visualization of the amniotic fluid flow in the vagina. If not, clinicians can use bedside tests detecting eithe...

journal_title：Annales de biologie clinique

authors： Julien M,N'guema L,Bouzerara A,Toro B,Lecarpentier E,Guibourdenche J

更新日期：2018-06-01 00:00:00

abstract：AIMS:To evaluate the effect of the physical activity on bone turnover in young male soccer players at the Tanner's stage of 1-2. MATERIAL AND METHODS:61 young soccer players (13,4 +/- 0,3 years old) who actively participated in soccer since 3,7 +/- 0,7 years were compared to 60 age and sex- matched non active subjects...

journal_title：Annales de biologie clinique

authors： Kalaï E,Bahlous A,Nbigh A,Sahli H,Sellami S,Abdelmoula J

更新日期：2007-09-01 00:00:00

abstract：:The aim of this study was to evaluate a new automated fluorimetric enzyme immunoassay for CK-MB using the Vidas bioMérieux. The results for sera from 222 patients were compared with those obtained using two other methods of CK-MB determination in mass units (Stratus II Dade, Magic Lite Analyzer II Ciba Corning). Satis...

journal_title：Annales de biologie clinique

authors： Ayed SB,Boukezia N,Foglietti MJ,Bernard M

更新日期：1996-01-01 00:00:00

abstract：:The aim of this study is to present an evaluation of HbA(1c) Assay on Architect CI8200 (Abbott Diagnostic). The measurement includes Hb assay by colorimetry and HbA(1c) by immunoturbidimetry. The percentage of HbA(1c) is the report HbA(1c)/complete Hb with a conversion coefficient. Repetability (n = 30; CV: 1.15-1.91%...

journal_title：Annales de biologie clinique

authors： Beaune G,Ducruet J,Jund J,Favre S

更新日期：2009-01-01 00:00:00

abstract：:The aim of the study was to check if a slight and non visible hemolysis to naked eye such as that induced by blood coagulation could interfere in the immunonephelometric measurement of haptoglobin, and if this interference was dependent on the protein phenotype. Results confirmed that blood coagulation induced a non v...

journal_title：Annales de biologie clinique

authors： Guéye PM,Sall I,Lessinger JM,Férard G

更新日期：2004-11-01 00:00:00

abstract：:Twenty four strains of coagulase-negative staphylococci from clinically significant blood cultures were separated in four groups on the basis of oxacillin susceptibility and phenotype of resistance to aminoglycoside antibiotics. Susceptibility testing included disk diffusion testing, M.I.C. and M.B.C. determinations a...

journal_title：Annales de biologie clinique

authors： Truchet A,Minet J,Mamez C,Cormier M

更新日期：1990-01-01 00:00:00

abstract：:The simultaneous presence of homogeneous and heterogeneous reactions at different binding sites of a multiepitope antigen makes the description of the kinetic parameters of the so called "one step" solid phase immunometric assays complex. The authors extended the "one step" approach to the concept of the "soluble sand...

journal_title：Annales de biologie clinique

authors： Chieregatti G,Massaglia A,Giraudi G,Zannino M,Barbieri U,Boniolo A

更新日期：1990-01-01 00:00:00

abstract：:Lungs can be the target of many fungal parasitic organisms. Some of them are specific to the lung (Pneumocystis); while others will migrate there in the course of their life cycle (Ascaris) or be caught in it (Paragonimus, Echinococcus). Sometimes, they also generate hypersensitivity reactions (fungi). In making a dia...

journal_title：Annales de biologie clinique

authors： De Gentile L,Chabasse D

更新日期：1990-01-01 00:00:00

abstract：:The authors describe a method of simultaneous estimation of phenobarbitone, mephenytoin and diphenylhydantoin in the blood. After extraction with ether, the methylation of these substances by trimethylanilinium hydroxide permits their rapid separation by gas chromatography. Heptabarbitone is used as internal standard ...

journal_title：Annales de biologie clinique

authors： Maitrot B,Hue G,Canonne MA,Rocher A,Landriau N

更新日期：1976-01-01 00:00:00

abstract：:Leukotrienes constitute a class of potent biological mediators of inflammation and anaphylaxis. However, their routine assay in various biological fluids is restricted by the complexity of the methodology. Previously this could only be performed by research laboratories with high pressure liquid chromatography and rad...

journal_title：Annales de biologie clinique

authors： Chabane MH,Le Vraux V,Le Provost AM,Leynadier F

更新日期：1995-01-01 00:00:00

abstract：:The present importance of streptococcal pathology implicates the identification of the streptococcal groups. The authors have carried out a comparative study of the various methods of extraction of the group specific antigen. They demonstrated the advantages and disadvantages of each method and consider the pronase ex...

journal_title：Annales de biologie clinique

authors： Bergogne-Berezin E,Slim A

更新日期：1976-01-01 00:00:00

abstract：:This article aims to place the phosphatidylethanol (PEth) blood test in the detection area of ethanol consumption causing alcohol-related disorders, to present the current methods of analysis, data on interpretation, some practical applications and the prospects of use of this biomarker. PEth is a minor metabolite of ...

journal_title：Annales de biologie clinique

authors： Hakim F,Wiart JF,Ménard O,Allorge D,Gaulier JM

更新日期：2019-12-01 00:00:00

abstract：:The relations between chromosomal diseases and mental retardation can be envisaged in three different ways: 1) Pharmacological approach shows a peculiar sensitivity of the cholinergic system in trisomic 21 children. A trouble in the manufacture of cholinergic mediator is plausible. 2) General biochemistry shows a slig...

journal_title：Annales de biologie clinique

authors： Lejeune J

更新日期：1978-01-01 00:00:00

abstract：:The purpose of this study is to find, besides the determination of lactate in blood, a suitable and specific indicator of brain damage in newborn. Accordingly, we studied the total creatine kinase (CK) activity, by the method of Rosalki, and its subunit isoenzymes BB (CK BB) by fluorescence following electrophoresis o...

journal_title：Annales de biologie clinique

authors： Chopin N,Lavaud J,Voyer M,Bally M,Cloup M

更新日期：1980-01-01 00:00:00

abstract：:Anti-citrullinated cyclic peptide antibodies (ACPA) were initially considered very specific for the diagnosis of rheumatoid arthritis (RA), and can predict the prognosis of the disease. However, these antibodies can be detected in other autoimmune diseases, including systemic lupus erythematosus (SLE), the most common...

journal_title：Annales de biologie clinique

authors： Bouchedoub Y,Djenouhat K,Rachedi N,Babasaci R,Ould Ali L,Salah K,Kherrache R,Kherbeche M,Khlifati A,Guernou FZ,Benzitouni A,Semmana M,Meghlaoui A

更新日期：2020-04-01 00:00:00

abstract：:Primary cancer of the liver, especially common in inter-tropical Africa and South-East Asia, still remains inaccessible to a really effective therapy, except for a rapid surgical excision. Improvement of its particularly poor prognosis requires therefore early screening based on reliable biological markers. Following ...

journal_title：Annales de biologie clinique

authors： Deugnier Y,David V,Leray G,Blayau M,Le Gall JY

更新日期：1988-01-01 00:00:00

abstract：:The aging process is an universal phenomenon. All individuals of a given species are dead by some age characteristic of the species. In spite of many biological and clinical observations on the changes that occur during aging, understanding about the causes of aging or the mechanisms determining "the life span potenti...

journal_title：Annales de biologie clinique

authors： Crastes de Paulet A

更新日期：1990-01-01 00:00:00

abstract：:In order to increase the versatility of phage display technology, it is desirable to be able to impose some structural constraints on the peptides that are presented by the phage particles. This is currently not feasible since the conformation of the capsid proteins, used to link the foreign peptide to the phage, are ...

journal_title：Annales de biologie clinique

authors： Santiago Vispo N,Felici F,Castagnoli L,Cesareni G

更新日期：1993-01-01 00:00:00

abstract：:Urinary ammonium excretion is the best parameter to quantify net acid excretion by the kidney. As measurement of ammonium excretion is not routinely available, clinicians use formulas to estimate NH(4)+ excretion. However, the measurement of urinary NH(4)+ concentration can be performed by an automatically method, whi...

journal_title：Annales de biologie clinique

authors： Szmidt-Adjidé V,Vanhille P

更新日期：2008-07-01 00:00:00

abstract：:Recent advances in the characterization of microcrystals in tissue samples based on physico-chemical techniques are reviewed. It is a new opportunity for the physician to access early a diagnosis of rare but severe pathologies and to start a specific therapy able to delay or to stop an irreversible alteration of the o...

journal_title：Annales de biologie clinique

authors： Bazin D,Daudon M

更新日期：2015-09-01 00:00:00

abstract：:HTLV genomic and antigenic features, replication way as well as associated pathology are recalled herein. The epidemiologic angle and the different transmission ways are also related. HTLV infection diagnostic implements are detailed: screening and specially confirmatory tests are brought to light with the help of con...

journal_title：Annales de biologie clinique

authors： Maniez-Montreuil M

更新日期：1998-09-01 00:00:00

abstract：:The aim of the present work was to assess the genetic and antigenic variability in the VP1 region of type 3 echviruses (E-3), an enterovirus serotype associated to meningitis, neuro-muscular diseases and type 1 diabetes in human. Forty-six VP1 sequences of E-3, among which 9 were isolated in tunisian infants, were inc...

journal_title：Annales de biologie clinique

authors： Farès W,Rezig D,Yahia AB,Touzi H,Meddeb Z,Triki H

更新日期：2012-03-01 00:00:00

abstract：:Laboratory diagnosis of pheochromocytoma must give evidence of increased catecholamine production. This requires measurement of catecholamines and their metabolites (normetanephrine NMN, metanephrine MN and/or VMA) in urine or in plasma. The various assays can be also performed during dynamic test that stimulate or in...

journal_title：Annales de biologie clinique

authors： Peyrin L,Mornex R

更新日期：1993-01-01 00:00:00

abstract：:Paroxysmal nocturnal hemoglobinuria (PNH) is a rare acquired hematopoietic stem cell disorder that must be correctly diagnose because it is a chronic disease with a real impact on the quality of life and the survival of the patients. PNH screening of all patients with anemia or thrombosis is not recommended. We report...

journal_title：Annales de biologie clinique

authors： Gendron N,Arlet JB,Gaussem P,Radford-Weiss I,Dupeux S,Rosain J,La Tour RP,Darnige L

更新日期：2017-10-01 00:00:00

abstract：:A fluorimetric, authomatical method for determination of free and total cholesterol in serum is described. Cholesterol esters are splitted into free cholesterol and fatty acids by cholesterol esterase. In the presence of oxygen free cholesterol will be transformed by cholesterol oxydase in delta 4 cholestenone with fo...

journal_title：Annales de biologie clinique

authors： Fruchart JC,Tsumbu BB,Dewailly P,Sezille G,Jaillard J

更新日期：1975-01-01 00:00:00