Abstract:
:Although some single polymorphism analyses of the angiotensinogen (AGT) gene have been found to be associated with hypertension, the results are still inconsistent. The objectives of this study are to evaluate the association of the genotype and haplotype distributions of three single-nucleotide polymorphisms (SNPs) (G-217A, A-6G, and M235T) in the AGT gene with hypertension. In a sample of 461 hypertensive and 327 normotensive patients in Taiwan, we found that -217AA and -6GG homozygotes conferred independently an increased risk to hypertension (P = 0.008 and P = 0.037, respectively), as illustrated by their significant associations with hypertension in both single SNP and pair-wise SNPs analyses. Meanwhile, a very weak linkage disequilibrium was found between the G-217A and the A-6G polymorphisms in terms of r2 (<0.05). On the basis of likelihood ratio test, only the set of haplotypes that constituted the A-6G and the M235T polymorphisms was associated with hypertension (chi2 = 20.91, P = 0.0008), which was mainly due to the increased frequency of the recombinant haplotypes (-6A identical with 235M and -6G identical with 235T), and a pathophysiological role in the predisposition to hypertension was hence indicated. In functional assays, the promoter activities of the haplotypes -217A identical with -6A and -217G identical with -6G were significantly higher than the most common haplotype -217G identical with -6A. These results highlight the necessity of a thorough analysis of all reported variants of a candidate gene in the elucidation of genetic susceptibility to a complex disease like hypertension, even when the variants are in the same haplotype block.
journal_name
Physiol Genomicsjournal_title
Physiological genomicsauthors
Wu SJ,Chiang FT,Chen WJ,Liu PH,Hsu KL,Hwang JJ,Lai LP,Lin JL,Tseng CD,Tseng YZdoi
10.1152/physiolgenomics.00133.2003subject
Has Abstractpub_date
2004-04-13 00:00:00pages
79-86issue
2eissn
1094-8341issn
1531-2267pii
00133.2003journal_volume
17pub_type
杂志文章abstract::The genetic basis of susceptibility to pulmonary fibrosis is largely unknown. Initially, in this study, loci regulating the response of bleomycin-induced pulmonary fibrosis were mapped using a set of recombinant congenic strains bred from pulmonary fibrosis-resistant A/J and susceptible C57BL/6J (B6) mice. Linkage was...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00095.2005
更新日期:2005-09-21 00:00:00
abstract::Recombinant human erythropoietin (rHuEPO) is frequently abused by athletes as a performance-enhancing drug, despite being prohibited by the World Anti-Doping Agency. Although the methods to detect blood doping, including rHuEPO injections, have improved in recent years, they remain imperfect. In a proof-of-principle s...
journal_title:Physiological genomics
pub_type: 临床试验,杂志文章
doi:10.1152/physiolgenomics.00108.2015
更新日期:2016-03-01 00:00:00
abstract::Chronic kidney disease (CKD), defined as reduced glomerular filtration rate, is increasingly becoming a major public health issue. At the histological level, renal fibrosis is the final common pathway leading to end-stage renal disease, irrespective of the initial injury. According to this view, antifibrotic agents sh...
journal_title:Physiological genomics
pub_type: 杂志文章,评审
doi:10.1152/physiolgenomics.00039.2017
更新日期:2018-01-01 00:00:00
abstract::Mast cell numbers are significantly increased in bladder disorders including malignancy and interstitial cystitis, but their precise role has been difficult to determine. We characterized the role of mast cells on gene regulation associated with antigen-induced bladder inflammation in mice. For this purpose, we examin...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00044.2001
更新日期:2001-10-10 00:00:00
abstract::The Ala allele of PPARG Pro12Ala ( rs1801282 ) is associated with greater improvements to the glucose metabolism in exercise studies, but whether this extends to peripheral insulin sensitivity is unknown. Our objective was to investigate the effect of PPARG Pro12Ala on exercise-induced changes in peripheral insulin se...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00101.2018
更新日期:2019-06-01 00:00:00
abstract::To account for sex as a biological variable, it is sometimes necessary to identify the sex of an embryo or embryonic cell that was used to generate libraries for RNA sequencing, without the sex being known a priori. The preferred approach for this would take advantage of the mRNA data, rather than relying on other met...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00001.2018
更新日期:2018-08-01 00:00:00
abstract::Following vascular injury medial smooth muscle cells dedifferentiate and migrate through the internal elastic lamina where they form a neointima. The goal of the current study was to identify changes in gene expression that occur before the development of neointima and are associated with the early response to injury....
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00095.2016
更新日期:2017-03-01 00:00:00
abstract::Several critical cell functions are influenced not only by internal cellular machinery but also by external mechanical and biochemical cues from the surrounding microenvironment. Slight changes to the microenvironment can result in dramatic changes to the cell's phenotype; for example, a change in the nutrients or pH ...
journal_title:Physiological genomics
pub_type: 杂志文章,评审
doi:10.1152/physiolgenomics.00170.2013
更新日期:2014-05-01 00:00:00
abstract::A high-fat (HF) diet is associated with progression of liver diseases. To illustrate genome-wide landscape of DNA methylation in liver of rats fed either a control or HF diet, two enrichment-based methods, namely methyl-DNA immunoprecipitation assay with high-throughput sequencing (MeDIP-seq) and methylation-sensitive...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00110.2014
更新日期:2015-10-01 00:00:00
abstract::The full extent to which 1,25-dihydroxyvitamin D(3) affects gene expression in human intestinal epithelial cells is unknown. We used oligonucleotide arrays to catalog vitamin D-induced changes in gene expression in Caco-2 cells, a human colon carcinoma cell line. Five paired sets of Caco-2 cell cultures were subjected...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00002.2003
更新日期:2004-04-13 00:00:00
abstract::Peroxisome proliferator-activated receptor type gamma (PPARgamma) is a subgroup of the PPAR transcription factor family. Recent studies indicate that loss of PPARgamma is associated with the development of pulmonary hypertension (PH). We hypothesized that the endothelial dysfunction associated with PPARgamma inhibitio...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00094.2009
更新日期:2009-12-30 00:00:00
abstract::The SMXA-5 recombinant inbred strain, which was established from nondiabetic parental SM/J and A/J mice, develops diabetic phenotypes such as impaired glucose tolerance. The combination of diabetogenic genes in the SM/J and A/J genomes impairs glucose tolerance in SMXA-5 mice. Using (SM/J x SMXA-5)F2 mice fed a high-f...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00027.2008
更新日期:2008-09-17 00:00:00
abstract::Systemic lupus erythematosus (SLE) is an autoimmune disorder with immune-complex deposition that affects multiple organs. Previous studies have suggested the involvement of oxidative stress and apoptosis in SLE, but no clear link to etiology has been established. Here we show that mice deficient in a transcription fac...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00209.2003
更新日期:2004-08-11 00:00:00
abstract::Myosin heavy chain genes (MYHs) are the most important functional domains of myosins, which are highly conserved throughout evolution. The human genome contains 15 MYHs, whereas the corresponding number in teleost appears to be much higher. Although teleosts comprise more than one-half of all vertebrate species, our k...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00278.2006
更新日期:2007-12-19 00:00:00
abstract::There exists a sense of urgency to begin to generate a cohesive assembly of biomedical knowledge as the pace of knowledge accumulation accelerates. The urgency is in part driven by the emergence of systems molecular medicine that emphasizes the combination of systems analysis and molecular dissection in the future of ...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00002.2007
更新日期:2007-08-20 00:00:00
abstract::A single point mutation in a novel immune-associated nucleotide gene 5 (Ian5) coincides with severe T cell lymphopenia in BB rats. We used a transgenic rescue approach in lymphopenic BB-derived congenic F344.lyp/lyp rats to determine whether this mutation is responsible for lymphopenia and to establish the functional ...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00126.2004
更新日期:2004-10-04 00:00:00
abstract::Morbidity and mortality associated with acute lung injury (ALI) and acute respiratory distress syndrome remain substantial. Although many candidate genes have been tested, a clear understanding of the pathogenesis is lacking, as is our ability to predict individual outcome. Because ALI is a complex disease, single gen...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.90392.2008
更新日期:2009-07-09 00:00:00
abstract::Cardiomyocytes derived from pluripotent embryonic stem cells (ESC) have the advantage of providing a source for standardized cell cultures. However, little is known on the regulation of the genome during differentiation of ESC to cardiomyocytes. Here, we characterize the transcriptome of the mouse ESC line CM7/1 durin...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.90287.2008
更新日期:2009-06-10 00:00:00
abstract::Herbivores are predicted to evolve appropriate mechanisms to process the plant secondary compounds (PSCs) in their diet, and these mechanisms are likely specific to particular suites of PSCs. Changes in diet composition over evolutionary time should select for appropriate alterations in metabolism of the more recent d...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00033.2013
更新日期:2013-09-03 00:00:00
abstract::Aside from abnormal angiogenesis, dual endothelin-1/VEGF signal peptide-activated receptor deficiency (DEspR(-/-)) results in aberrant neuroepithelium and neural tube differentiation, thus elucidating DEspR's role in neurogenesis. With the emerging importance of neurogenesis in adulthood, we tested the hypothesis that...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00044.2008
更新日期:2008-11-12 00:00:00
abstract::Although increased vascular stiffness is more prominent in aging males than females, and males are more prone to vascular disease with aging, no study has investigated the genes potentially responsible for sex differences in vascular aging. We tested the hypothesis that the transcriptional adaptation to aging differs ...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00229.2006
更新日期:2007-04-24 00:00:00
abstract::Adenosine deaminase acting on RNA (ADAR) in Drosophila and mammals has recently become the target of numerous investigations. It is now clear that this protein has a number of functions in the nervous system. Indeed, the mutation of ADAR in Drosophila (dADAR) results in many pathological and physiological changes, suc...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00093.2003
更新日期:2005-01-20 00:00:00
abstract::Studies were performed to examine the extent to which mechanical stimuli mediate control of angiogenesis in bladder cells both in vitro and in vivo. Differential gene expression between control nonstretched and cyclically stretched bladder smooth muscle cells was assessed using oligonucleotide microarrays and pathway ...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.90291.2008
更新日期:2008-12-12 00:00:00
abstract::Higher fat and lower carbohydrate and amino acid oxidation are observed in women compared with men during endurance exercise. We hypothesized that the observed sex difference is due to estrogen and that menstrual cycle phase or supplementation of men with 17beta-estradiol (E(2)) would coordinately influence the mRNA c...
journal_title:Physiological genomics
pub_type: 杂志文章,随机对照试验
doi:10.1152/physiolgenomics.00115.2009
更新日期:2009-12-30 00:00:00
abstract::Physiological, morphological, and transcriptional alterations elicited by ethynyl estradiol in the uteri of Sprague-Dawley rats and C57BL/6 mice were assessed using comparable study designs, microarray platforms, and analysis methods to identify conserved estrogen signaling networks. Comparative analysis identified 15...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00175.2005
更新日期:2005-11-17 00:00:00
abstract::Little is known regarding the graft response to transplantation injury. This study investigates the posttransplantation response of genes that are constitutively expressed in the heart. Constitutive heart and lymph node tissue-restricted gene expression was first analyzed with DNA microarrays. To demonstrate changes f...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00139.2002
更新日期:2003-09-29 00:00:00
abstract::The human miR-29 family of microRNAs has three mature members, miR-29a, miR-29b, and miR-29c. miR-29s are encoded by two gene clusters. Binding sites for several transcriptional factors have been identified in the promoter regions of miR-29 genes. The miR-29 family members share a common seed region sequence and are p...
journal_title:Physiological genomics
pub_type: 杂志文章,评审
doi:10.1152/physiolgenomics.00141.2011
更新日期:2012-02-27 00:00:00
abstract::The gastric entero-chromaffin-like (ECL) cell plays a key regulatory role in peripheral regulation of acid secretion due to the release of histamine that stimulates acid secretion by the parietal cell. Studies in intact animals, gastric glands, and isolated cells after short-term culture have shown expression of stimu...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00271.2005
更新日期:2006-03-13 00:00:00
abstract::Psoriasis is a chronic and common human skin disorder currently with no cure. Psoriatic skin displays inflammatory, raised, and scaly lesions with widely aberrant gene expression. Recent studies have revealed critical roles that microRNAs play as a class of posttranscriptional gene regulator in skin development and sk...
journal_title:Physiological genomics
pub_type: 杂志文章,评审
doi:10.1152/physiolgenomics.00157.2013
更新日期:2014-02-15 00:00:00
abstract::Cultured shrimp are continuously exposed to variable environmental conditions that have been associated with stress and subsequent outbreaks of disease. To investigate the effect of environmental stress on Penaeus monodon gene expression, a 3,853 random cDNA microarray chip was generated with clones originating from s...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00068.2007
更新日期:2007-09-19 00:00:00