Abstract:
:A single point mutation in a novel immune-associated nucleotide gene 5 (Ian5) coincides with severe T cell lymphopenia in BB rats. We used a transgenic rescue approach in lymphopenic BB-derived congenic F344.lyp/lyp rats to determine whether this mutation is responsible for lymphopenia and to establish the functional importance of this novel gene. A 150-kb P1 artificial chromosome (PAC) transgene harboring a wild-type allele of the rat Ian5 gene restored Ian5 transcript and protein levels, completely rescuing the T cell lymphopenia in the F344.lyp/lyp rats. This successful complementation provides direct functional evidence that the Ian5 gene product is essential for maintaining normal T cell levels. It also demonstrates that transgenic rescue in the rat is a practical and definitive method for revealing the function of a novel gene.
journal_name
Physiol Genomicsjournal_title
Physiological genomicsauthors
Michalkiewicz M,Michalkiewicz T,Ettinger RA,Rutledge EA,Fuller JM,Moralejo DH,Van Yserloo B,MacMurray AJ,Kwitek AE,Jacob HJ,Lander ES,Lernmark Adoi
10.1152/physiolgenomics.00126.2004subject
Has Abstractpub_date
2004-10-04 00:00:00pages
228-32issue
2eissn
1094-8341issn
1531-2267pii
00126.2004journal_volume
19pub_type
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