Abstract:
:It has been found that Arabian and Thoroughbred horses differ in muscle fiber structure and thus in physiological changes occurring in muscles during exercise. The aim of the present study was to identify the global gene expression modifications that occur in skeletal muscle following a training regime to prepare for flat racing. Whole transcriptomes of muscle (gluteus medius) were compared between three time points of tissue collection: T0 (untrained horses), T1 (horses after intense gallop phase), and T2 (horses at the end of racing season), 23 samples in total. The numerous groups of exercise-regulated differentially expressed genes (DEGs) were related to muscle cell structure and signaling and included insulin-like growth factor 1 receptor (IGF1R), insulin receptor (INSR), transforming growth factor beta receptors 1 and 2 (TGFBR1, TGFBR2), vascular endothelial growth factor B (VEGFB); epidermal growth factor (EGF), hepatocyte growth factor (HGF), and vascular endothelial growth factor D (FIGF). In Arabian horses, exercise modified the expression of genes belonging to the PPAR signaling pathway (e.g., PPARA, PPARD, and PLIN2), calcium signaling pathway, and pathways associated with metabolic processes (e.g., oxidative phosphorylation, fatty acid metabolism, glycolysis/gluconeogenesis, and citrate cycle). According to detected gene expression modifications, our results suggested that in Arabian horses, exercise switches energy generation toward fatty acid utilization and enhances glycogen transport and calcium signaling. The use of the RNA-Seq approach in analyzing the skeletal muscle transcriptome allowed for the proposal of a panel of new candidate genes potentially related to body homeostasis maintenance and racing performance in Arabian horses.
journal_name
Physiol Genomicsjournal_title
Physiological genomicsauthors
Ropka-Molik K,Stefaniuk-Szmukier M,Z Ukowski K,Piórkowska K,Bugno-Poniewierska Mdoi
10.1152/physiolgenomics.00130.2016subject
Has Abstractpub_date
2017-06-01 00:00:00pages
318-326issue
6eissn
1094-8341issn
1531-2267pii
physiolgenomics.00130.2016journal_volume
49pub_type
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