Abstract:
:Genetic analysis of polygenic traits in rats and mice has been very useful for finding the approximate chromosomal locations of the genes causing quantitative phenotypic variation, so-called quantitative trait loci (QTL). Further localization of the causative genes and their ultimate identification has, however, proven to be slow and frustrating. A major technique for gene identification in such models utilizes series of congenic strains with progressively smaller chromosomal segments introgressed from one inbred strain into another inbred strain. Under the assumption that a single causative locus underlies a QTL, nested series of congenic strains were earlier suggested as an appropriate configuration for the congenic strains. It is now known that most QTL are compound, that is, the QTL signal is caused by clusters of loci where alleles exert positive, negative, and interactive effects on the trait in a given strain comparison. It is argued that in this situation an initial series of nonoverlapping contiguous congenic strains over a relatively large chromosomal region will lead to a better appreciation of the underlying complexity of the QTL and therefore more rapid gene identification. Examples from the literature where this strategy would be helpful, as well as a case where it would be potentially counterproductive, are given.
journal_name
Physiol Genomicsjournal_title
Physiological genomicsauthors
Rapp JP,Joe Bdoi
10.1152/physiolgenomics.00136.2011subject
Has Abstractpub_date
2012-02-01 00:00:00pages
117-20issue
2eissn
1094-8341issn
1531-2267pii
physiolgenomics.00136.2011journal_volume
44pub_type
杂志文章abstract::Septa from sheep hearts at 130 days gestation, term, and 14-day-old lambs were used to model the changes in gene expression patterns during the perinatal period using Agilent 15k ovine microarrays. We used Bioconductor for R to model five major patterns of coexpressed genes. Gene ontology and transcription factor anal...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00027.2015
更新日期:2015-09-01 00:00:00
abstract::To better understand the genetic basis of essential hypertension, we conducted a quantitative trait locus (QTL) analysis of a population of 207 (BALB/cJ x CBA/CaJ) F(2) male mice to identify genomic regions that regulate blood pressure, heart rate, and heart weight. We identified two loci, Bpq6 (blood pressure quantit...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00002.2002
更新日期:2002-07-12 00:00:00
abstract::The structural organization of the brain is important for normal brain function and is critical to understand in order to evaluate changes that occur during disease processes. Three-dimensional (3D) imaging of the mouse brain is necessary to appreciate the spatial context of structures within the brain. In addition, t...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00055.2012
更新日期:2012-08-01 00:00:00
abstract::Large datasets from -omics studies need to be deeply investigated. The aim of this paper is to provide a new method (LEM method) for the search of transcriptome and metabolome connections. The heuristic algorithm here described extends the classical canonical correlation analysis (CCA) to a high number of variables (w...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00139.2012
更新日期:2013-07-15 00:00:00
abstract::Slc22 family organic anion and cation transporters (OATs, OCTs, and OCTNs) are transmembrane proteins expressed predominantly in kidney and liver. These proteins mediate the uptake or excretion of numerous physiologically (and pharmacologically) important compounds, and accordingly have been the focus of intensive stu...
journal_title:Physiological genomics
pub_type: 杂志文章,评审
doi:10.1152/physiolgenomics.00014.2004
更新日期:2004-06-17 00:00:00
abstract::Repair and regeneration are key processes for tissue maintenance, and their disruption may lead to disease states. Little is known about the molecular mechanisms that underline the repair and regeneration of the digestive tract. The sea cucumber Holothuria glaberrima represents an excellent model to dissect and charac...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00228.2006
更新日期:2007-10-22 00:00:00
abstract::RNA viruses represent the predominant cause of many clinically relevant viral diseases in humans. Among several evolutionary advantages acquired by RNA viruses, the ability to usurp host cellular machinery and evade antiviral immune responses is imperative. During the past decade, RNA interference mechanisms, especial...
journal_title:Physiological genomics
pub_type: 杂志文章,评审
doi:10.1152/physiolgenomics.00112.2013
更新日期:2013-11-15 00:00:00
abstract::The relative importance of regulatory versus structural evolution for the evolution of different biological systems is a subject of controversy. The primacy of regulatory evolution in the diversification of morphological traits has been promoted by many evolutionary developmental biologists. For physiological traits, ...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00159.2007
更新日期:2008-11-12 00:00:00
abstract::Preeclampsia is a progressive hypertensive disorder of pregnancy affecting 2-8% of pregnancies globally. Preexisting chronic hypertension is a major risk factor associated with developing preeclampsia, and growing evidence suggests a role for the gut microbiome in the development of preeclampsia. However, neither alte...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00121.2020
更新日期:2021-01-25 00:00:00
abstract::Psoriasis is a chronic and common human skin disorder currently with no cure. Psoriatic skin displays inflammatory, raised, and scaly lesions with widely aberrant gene expression. Recent studies have revealed critical roles that microRNAs play as a class of posttranscriptional gene regulator in skin development and sk...
journal_title:Physiological genomics
pub_type: 杂志文章,评审
doi:10.1152/physiolgenomics.00157.2013
更新日期:2014-02-15 00:00:00
abstract::This study analyzes the electrophysiological cause and behavioral consequence of dopaminergic cell loss in a knockin mouse strain bearing hypersensitive nicotinic alpha4-receptor subunits ("L9'S mice"). Adult brains of L9'S mice show moderate loss of substantia nigra dopaminergic neurons and of striatal dopaminergic i...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00012.2004
更新日期:2004-08-11 00:00:00
abstract::There exists a sense of urgency to begin to generate a cohesive assembly of biomedical knowledge as the pace of knowledge accumulation accelerates. The urgency is in part driven by the emergence of systems molecular medicine that emphasizes the combination of systems analysis and molecular dissection in the future of ...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00002.2007
更新日期:2007-08-20 00:00:00
abstract::We previously reported that mice deficient in stearoyl-CoA desaturase-1 (Scd1) and maintained on a very low-fat (VLF) diet for 10 days developed severe loss of body weight, hypoglycemia, hypercholesterolemia, and many cholestasis-like phenotypes. To better understand the metabolic changes associated with these phenoty...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00139.2007
更新日期:2008-05-13 00:00:00
abstract::Aortic banding in the rat has become a popular method to induce left ventricular (LV) hypertrophy and heart failure. However, because of often extensive intrathoracic adhesions and inflammatory cell infiltrates resulting from the traditional surgical approach, an uncomplicated second thoracic incision for genetic mani...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00035.2001
更新日期:2002-01-01 00:00:00
abstract::To investigate the transcriptional response to oxidative stress in the heart and how it changes with age, we examined the cardiac gene expression profiles of young (5-mo-old), middle-aged (15-mo-old), and old (25-mo-old) C57BL/6 mice treated with a single intraperitoneal injection of paraquat (50 mg/kg). Mice were kil...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00172.2002
更新日期:2003-04-16 00:00:00
abstract::Following vascular injury medial smooth muscle cells dedifferentiate and migrate through the internal elastic lamina where they form a neointima. The goal of the current study was to identify changes in gene expression that occur before the development of neointima and are associated with the early response to injury....
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00095.2016
更新日期:2017-03-01 00:00:00
abstract::High cardiorespiratory fitness (CRF) is associated with a reduced risk of Type 2 diabetes mellitus (T2DM) and improved β-cell function; genetic factors also determine these risks. This cross-sectional study investigated whether CRF modifies the association of polygenic risk of T2DM with glucose metabolism in nondiabet...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00027.2014
更新日期:2014-07-15 00:00:00
abstract::alpha-Sarcoglycan-deficient (Sgca-null) mice provide potential for elucidating the pathogenesis of limb girdle muscular dystrophy type 2D (LGMD 2D) as well as for studying the effectiveness of therapeutic strategies. Skeletal muscles of Sgca-null mice demonstrate an early onset of extensive fiber necrosis, degeneratio...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00311.2004
更新日期:2005-07-14 00:00:00
abstract::The mammalian liver works to keep the body in a state of homeostasis and plays an important role in systemic acute phase response to infections. In this study we investigated the bovine hepatic acute phase response at the gene transcription level in dairy cows with experimentally Escherichia coli-induced mastitis. At ...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00084.2011
更新日期:2012-06-01 00:00:00
abstract::We previously constructed a congenic mouse, B6.S-D2Mit194-D2Mit311 (B6.S-2) with 27 Mb of SPRET/Ei donor DNA on distal chromosome 2 in a C57BL/6J background that captured an obesity quantitative trait locus (QTL). Mice homozygous for SPRET/Ei alleles at the donor region had decreased body weight and obesity-related ph...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00267.2006
更新日期:2007-09-19 00:00:00
abstract::A high-fat (HF) diet is associated with progression of liver diseases. To illustrate genome-wide landscape of DNA methylation in liver of rats fed either a control or HF diet, two enrichment-based methods, namely methyl-DNA immunoprecipitation assay with high-throughput sequencing (MeDIP-seq) and methylation-sensitive...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00110.2014
更新日期:2015-10-01 00:00:00
abstract::The genetically hypertensive fawn-hooded (FHH/Eur) rat is characterized by the early presence of systolic and glomerular hypertension, progressive proteinuria (UPV), and albuminuria (UAV), and focal glomerulosclerosis, resulting in premature death from renal failure. Previous studies showed that at least five genetic ...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00030.2000
更新日期:2002-02-28 00:00:00
abstract::In type 1 diabetes mellitus (T1DM), also known as autoimmune diabetes, the pathogenic destruction of the insulin-producing pancreatic beta-cells is under the control of and influenced by distinct subsets of T lymphocytes. To identify the critical genes expressed by autoimmune T cells, antigen presenting cells, and pan...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00173.2004
更新日期:2005-04-14 00:00:00
abstract::The SMXA-5 recombinant inbred strain, which was established from nondiabetic parental SM/J and A/J mice, develops diabetic phenotypes such as impaired glucose tolerance. The combination of diabetogenic genes in the SM/J and A/J genomes impairs glucose tolerance in SMXA-5 mice. Using (SM/J x SMXA-5)F2 mice fed a high-f...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00027.2008
更新日期:2008-09-17 00:00:00
abstract::Although the genetics of rare, monogenic, forms of human hypertension are fairly well defined, the genetics of the common polygenic form of human essential hypertension is only emerging. With the ability to control environmental variables, animal models have provided valuable tools with which to study blood pressure (...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00027.2003
更新日期:2003-11-11 00:00:00
abstract::Although Escherichia coli LPS is known to elicit various proinflammatory responses in macrophages, its effect on the translational states of transcripts has not yet been explored on a genome-wide scale. To address this, we investigated the mRNA profiles in polysomal and free messenger ribonucleoprotein particle (mRNP)...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00095.2007
更新日期:2008-03-14 00:00:00
abstract::Caloric restriction (CR) is an effective method for prevention of age-associated diseases as well as overweight and obesity; however, there is controversy regarding the effects of dieting regimens on behavior. In this study, we investigated two different dieting regimens: repeated fasting and refeeding (RFR) and daily...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00082.2009
更新日期:2009-11-06 00:00:00
abstract::The mammary gland undergoes extensive remodeling between the beginning of pregnancy and lactation; this involves cellular processes including cell proliferation, differentiation, and apoptosis, all of which are under the control of numerous regulators. To unravel the role played by miRNA, we describe here 47 new ovine...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00091.2012
更新日期:2013-02-15 00:00:00
abstract::An increased phospholamban (PLB)-to-sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA) ratio has been suggested to contribute to the slowing of relaxation in failing human ventricle. We have used an adenoviral vector carrying the sequence for PLB to increase this ratio in isolated adult rat ventricular myocytes, and w...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.1999.1.2.41
更新日期:1999-08-31 00:00:00
abstract::Hypoxia-inducible factor-1alpha (HIF-1alpha) is a transcription factor that regulates the oxygen-dependent expression of a number of genes. This transcription factor may contribute to the abundant expression of many genes in renal medullary cells that function normally under hypoxic conditions. The present study was d...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.2001.6.3.159
更新日期:2001-08-28 00:00:00