Abstract:
:The zebrafish has become a popular model system for the study of vertebrate developmental biology because of its numerous strengths as a molecular genetic and embryological system. To determine the requirement for specific genes during embryogenesis, it is necessary to generate organisms carrying loss-of-function mutations. This can be accomplished in zebrafish through a reverse genetic approach. This review discusses the current techniques for generating mutations in known genes in zebrafish. These techniques include the generation of chromosomal deletions and the subsequent identification of complementation groups within deletions through noncomplementation assays. In addition, this review will discuss methods currently being evaluated that may improve the methods for finding mutations in a known sequence, including screening for randomly induced small deletions within genes and screening for randomly induced point mutations within specific genes.
journal_name
Physiol Genomicsjournal_title
Physiological genomicsauthors
Lekven AC,Helde KA,Thorpe CJ,Rooke R,Moon RTdoi
10.1152/physiolgenomics.2000.2.2.37subject
Has Abstractpub_date
2000-03-13 00:00:00pages
37-48issue
2eissn
1094-8341issn
1531-2267pii
2/2/37journal_volume
2pub_type
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