HIF signaling and overall gene expression changes during hypoxia and prolonged exercise differ considerably.

Abstract:

:Exercise as well as hypoxia cause an increase in angiogenesis, changes in mitochondrial density and alterations in metabolism, but it is still under debate whether the hypoxia inducible factor (HIF) is active during both situations. In this study gene expression analysis of zebrafish larvae that were raised under normoxic, hypoxic, or training conditions were compared, using microarray analysis, quantitative real-time PCR and protein data. Although HIF expression is posttranslationally regulated, mRNA expression levels of all three isoforms (HIF-1α, HIF-2α, and HIF-3α) differed in each of the experimental groups, but the changes observed in hypoxic animals were much smaller than in trained larvae. Prominent changes were seen for Hif-2α expression, which significantly increased after the first day of exercise and then decreased down to values significantly below control values. HIF-3α mRNA expression in turn increased significantly, and at the end of the training period (9-15 days postfertilization) it was elevated three times. At the protein level a transient increase in HIF-1α was observed in hypoxic larvae, whereas in the exercise group the amount of HIF-1α protein even decreased below the level of control animals. The analyzed transcriptome was more affected in hypoxic zebrafish larvae, and hardly any genes were similarly altered by both treatments. These results clearly showed that HIF proteins played different roles in trained and hypoxic zebrafish larvae and that the exercise-induced transition to a more aerobic phenotype was not achieved by persistent activation of the hypoxic signaling pathway.

journal_name

Physiol Genomics

journal_title

Physiological genomics

authors

Kopp R,Köblitz L,Egg M,Pelster B

doi

10.1152/physiolgenomics.00250.2010

subject

Has Abstract

pub_date

2011-05-13 00:00:00

pages

506-16

issue

9

eissn

1094-8341

issn

1531-2267

pii

physiolgenomics.00250.2010

journal_volume

43

pub_type

杂志文章
  • Exercise-induced changes in insulin action are associated with ACE gene polymorphisms in older adults.

    abstract::We evaluated the association between insulin resistance and the angiotensin-converting enzyme (ACE) insertion (I)/deletion (D) gene polymorphism in a group of older hypertensive subjects (63 +/- 1 yr, n = 35) before and after a 6-mo aerobic exercise program (AEX). Insulin sensitivity index (S(I)), assessed by the freq...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00048.2002

    authors: Dengel DR,Brown MD,Ferrell RE,Reynolds TH 4th,Supiano MA

    更新日期:2002-10-29 00:00:00

  • Ammonia excretion in rainbow trout (Oncorhynchus mykiss): evidence for Rh glycoprotein and H+-ATPase involvement.

    abstract::Branchial ammonia transport in freshwater teleosts is not well understood. Most studies conclude that NH(3) diffuses out of the gill and becomes protonated to NH(4)(+) in an acidified gill boundary layer. Rhesus (Rh) proteins are new members of the ammonia transporter superfamily and rainbow trout possess genes encodi...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00061.2007

    authors: Nawata CM,Hung CC,Tsui TK,Wilson JM,Wright PA,Wood CM

    更新日期:2007-11-14 00:00:00

  • High-fat diet negatively impacts both metabolic and behavioral health in outbred heterogeneous stock rats.

    abstract::Obesity is influenced by genetics and diet and has wide ranging comorbidities, including anxiety and depressive disorders. Outbred heterogeneous stock (HS) rats are used for fine-genetic mapping of complex traits and may be useful for understanding gene by diet interactions. In this study, HS rats were fed diets conta...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00018.2020

    authors: Deal AW,Seshie O,Lenzo A,Cooper N,Ozimek N,Solberg Woods LC

    更新日期:2020-09-01 00:00:00

  • Short-term administration of rhGH increases markers of cellular proliferation but not milk protein gene expression in normal lactating women.

    abstract::Growth hormone is one of few pharmacologic agents known to augment milk production in humans. We hypothesized that recombinant human GH (rhGH) increases the expression of cell proliferation and milk protein synthesis genes. Sequential milk and blood samples collected over four days were obtained from five normal lacta...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00079.2010

    authors: Maningat PD,Sen P,Rijnkels M,Hadsell DL,Bray MS,Haymond MW

    更新日期:2011-04-27 00:00:00

  • Evolution of ventricular myocyte electrophysiology.

    abstract::The relative importance of regulatory versus structural evolution for the evolution of different biological systems is a subject of controversy. The primacy of regulatory evolution in the diversification of morphological traits has been promoted by many evolutionary developmental biologists. For physiological traits, ...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00159.2007

    authors: Rosati B,Dong M,Cheng L,Liou SR,Yan Q,Park JY,Shiang E,Sanguinetti M,Wang HS,McKinnon D

    更新日期:2008-11-12 00:00:00

  • DEspR T/CATAAAA-box promoter variant decreases DEspR transcription and is associated with increased BP in Sardinian males.

    abstract::Essential hypertension is highly prevalent in the elderly population, exceeding 70% in people older than 60 yr of age, and remains a leading risk factor for heart disease, stroke, and chronic renal disease. Elucidation of genetic determinants is critical but remains a challenge due to its complex, multifactorial patho...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00012.2011

    authors: Glorioso N,Herrera VL,Didishvili T,Argiolas G,Troffa C,Bulla P,Bulla E,Ruiz-Opazo N

    更新日期:2011-11-07 00:00:00

  • The miR-29 family: genomics, cell biology, and relevance to renal and cardiovascular injury.

    abstract::The human miR-29 family of microRNAs has three mature members, miR-29a, miR-29b, and miR-29c. miR-29s are encoded by two gene clusters. Binding sites for several transcriptional factors have been identified in the promoter regions of miR-29 genes. The miR-29 family members share a common seed region sequence and are p...

    journal_title:Physiological genomics

    pub_type: 杂志文章,评审

    doi:10.1152/physiolgenomics.00141.2011

    authors: Kriegel AJ,Liu Y,Fang Y,Ding X,Liang M

    更新日期:2012-02-27 00:00:00

  • Identification of positional candidate genes for body weight and adiposity in subcongenic mice.

    abstract::We previously constructed a congenic mouse, B6.S-D2Mit194-D2Mit311 (B6.S-2) with 27 Mb of SPRET/Ei donor DNA on distal chromosome 2 in a C57BL/6J background that captured an obesity quantitative trait locus (QTL). Mice homozygous for SPRET/Ei alleles at the donor region had decreased body weight and obesity-related ph...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00267.2006

    authors: Chiu S,Kim K,Haus KA,Espinal GM,Millon LV,Warden CH

    更新日期:2007-09-19 00:00:00

  • Gene expression effects of lithium and valproic acid in a serotonergic cell line.

    abstract::Valproic acid (VPA) and lithium are widely used in the treatment of bipolar disorder. However, the underlying mechanism of action of these drugs is not clearly understood. We used RNA-Seq analysis to examine the global profile of gene expression in a rat serotonergic cell line (RN46A) after exposure to these two mood ...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00069.2018

    authors: Balasubramanian D,Pearson JF,Kennedy MA

    更新日期:2019-02-01 00:00:00

  • Gene selection heuristic algorithm for nutrigenomics studies.

    abstract::Large datasets from -omics studies need to be deeply investigated. The aim of this paper is to provide a new method (LEM method) for the search of transcriptome and metabolome connections. The heuristic algorithm here described extends the classical canonical correlation analysis (CCA) to a high number of variables (w...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00139.2012

    authors: Valour D,Hue I,Grimard B,Valour B

    更新日期:2013-07-15 00:00:00

  • Functional and structural characterization of the zebrafish Na+-sulfate cotransporter 1 (NaS1) cDNA and gene (slc13a1).

    abstract::Sulfate plays an essential role during growth, development and cellular metabolism. In this study, we characterized the function and structure of the zebrafish (Danio rerio) Na+-sulfate cotransporter 1 (NaS1) cDNA and gene (slc13a1). Zebrafish NaS1 encodes a protein of 583 amino acids with 13 putative transmembrane do...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.90234.2008

    authors: Markovich D,Romano A,Storelli C,Verri T

    更新日期:2008-08-15 00:00:00

  • Bone marrow mononuclear cells induce beneficial remodeling and reduce diastolic dysfunction in the left ventricle of hypertensive SS/MCWi rats.

    abstract::Bone marrow mononuclear cells (BMMNCs) increase capillary density and reduce fibrosis in rodents after myocardial infarction, resulting in an overall improvement in left ventricular function. Little is known about the effectiveness of BMMNC therapy in hypertensive heart disease. In the current study, we show that deli...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00170.2011

    authors: Parker SJ,Didier DN,Karcher JR,Stodola TJ,Endres B,Greene AS

    更新日期:2012-10-02 00:00:00

  • Use of contiguous congenic strains in analyzing compound QTLs.

    abstract::Genetic analysis of polygenic traits in rats and mice has been very useful for finding the approximate chromosomal locations of the genes causing quantitative phenotypic variation, so-called quantitative trait loci (QTL). Further localization of the causative genes and their ultimate identification has, however, prove...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00136.2011

    authors: Rapp JP,Joe B

    更新日期:2012-02-01 00:00:00

  • Transcriptome profiling and network analysis of genetically hypertensive mice identifies potential pharmacological targets of hypertension.

    abstract::Hypertension is a condition with major cardiovascular and renal complications, affecting nearly a billion patients worldwide. Few validated gene targets are available for pharmacological intervention, so there is a need to identify new biological pathways regulating blood pressure and containing novel targets for trea...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00010.2010

    authors: Puig O,Wang IM,Cheng P,Zhou P,Roy S,Cully D,Peters M,Benita Y,Thompson J,Cai TQ

    更新日期:2010-09-01 00:00:00

  • Transfer of the Rf-1 region from FHH onto the ACI background increases susceptibility to renal impairment.

    abstract::The genetically hypertensive fawn-hooded (FHH/Eur) rat is characterized by the early presence of systolic and glomerular hypertension, progressive proteinuria (UPV), and albuminuria (UAV), and focal glomerulosclerosis, resulting in premature death from renal failure. Previous studies showed that at least five genetic ...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00030.2000

    authors: Provoost AP,Shiozawa M,Van Dokkum RP,Jacob HJ

    更新日期:2002-02-28 00:00:00

  • Resequencing of IRS2 reveals rare variants for obesity but not fasting glucose homeostasis in Hispanic children.

    abstract::Our objective was to resequence insulin receptor substrate 2 (IRS2) to identify variants associated with obesity- and diabetes-related traits in Hispanic children. Exonic and intronic segments, 5' and 3' flanking regions of IRS2 (∼14.5 kb), were bidirectionally sequenced for single nucleotide polymorphism (SNP) discov...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00019.2011

    authors: Butte NF,Voruganti VS,Cole SA,Haack K,Comuzzie AG,Muzny DM,Wheeler DA,Chang K,Hawes A,Gibbs RA

    更新日期:2011-09-22 00:00:00

  • Transcription factor CHF1/Hey2 regulates the global transcriptional response to platelet-derived growth factor in vascular smooth muscle cells.

    abstract::The cardiovascular restricted transcription factor CHF1/Hey2 has been previously shown to regulate the smooth muscle response to growth factors. To determine how CHF1/Hey2 affects the smooth muscle response to growth factors, we performed a genomic screen for transcripts that are differentially expressed in wild-type ...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00277.2006

    authors: Shirvani SM,Mookanamparambil L,Ramoni MF,Chin MT

    更新日期:2007-06-19 00:00:00

  • Transcriptional reprogramming and ultrastructure during atrophy and recovery of mouse soleus muscle.

    abstract::This study investigated the use of the hindlimb suspension (HS) and reloading model of mice for the mapping of ultrastructural and gene expressional alterations underlying load-dependent muscular adaptations. Mice were hindlimb suspended for 7 days or kept as controls (n = 12). Soleus muscles were harvested after HS (...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00100.2004

    authors: Däpp C,Schmutz S,Hoppeler H,Flück M

    更新日期:2004-12-15 00:00:00

  • Integration of expression profiles and genetic mapping data to identify candidate genes in intracranial aneurysm.

    abstract::Intracranial aneurysm (IA) is a complex genetic disease for which, to date, 10 loci have been identified by linkage. Identification of the risk-conferring genes in the loci has proven difficult, since the regions often contain several hundreds of genes. An approach to prioritize positional candidate genes for further ...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00015.2007

    authors: Weinsheimer S,Lenk GM,van der Voet M,Land S,Ronkainen A,Alafuzoff I,Kuivaniemi H,Tromp G

    更新日期:2007-12-19 00:00:00

  • A novel genetic locus modulates infarct volume independently of the extent of collateral circulation.

    abstract::In the mouse model of permanent, middle cerebral artery occlusion, infarct volume varies widely across inbred strains but generally is inversely correlated with collateral vessel number. However, we also observed certain mouse strains that share similar collateral vessel anatomy but exhibit significantly different inf...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00063.2013

    authors: Chu PL,Keum S,Marchuk DA

    更新日期:2013-09-03 00:00:00

  • Deletion of a subgroup of ribosome-related genes minimizes hypoxia-induced changes and confers hypoxia tolerance.

    abstract::Hypoxia is a widely occurring condition experienced by diverse organisms under numerous physiological and disease conditions. To probe the molecular mechanisms underlying hypoxia responses and tolerance, we performed a genome-wide screen to identify mutants with enhanced hypoxia tolerance in the model eukaryote, the y...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00232.2010

    authors: Shah AN,Cadinu D,Henke RM,Xin X,Dastidar RG,Zhang L

    更新日期:2011-07-27 00:00:00

  • Hypothalamic gene expression profile indicates a reduction in G protein signaling in the Wfs1 mutant mice.

    abstract::The Wfs1 gene codes for a protein with unknown function, but deficiency in this protein results in a range of neuropsychiatric and neuroendocrine syndromes. In the present study we aimed to find the functional networks influenced by Wfs1 in the hypothalamus. We performed gene expression profiling (Mouse Gene 1.0 ST Ar...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00117.2011

    authors: Kõks S,Soomets U,Plaas M,Terasmaa A,Noormets K,Tillmann V,Vasar E,Fernandes C,Schalkwyk LC

    更新日期:2011-12-16 00:00:00

  • Thermoprotection of synaptic transmission in a Drosophila heat shock factor mutant is accompanied by increased expression of Hsp83 and DnaJ-1.

    abstract::In Drosophila larvae, acquired synaptic thermotolerance after heat shock has previously been shown to correlate with the induction of heat shock proteins (Hsps) including HSP70. We tested the hypothesis that synaptic thermotolerance would be significantly diminished in a temperature-sensitive strain (Drosophila heat s...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00195.2005

    authors: Neal SJ,Karunanithi S,Best A,So AK,Tanguay RM,Atwood HL,Westwood JT

    更新日期:2006-05-16 00:00:00

  • Transcriptomes of purified gastric ECL and parietal cells: identification of a novel pathway regulating acid secretion.

    abstract::The gastric entero-chromaffin-like (ECL) cell plays a key regulatory role in peripheral regulation of acid secretion due to the release of histamine that stimulates acid secretion by the parietal cell. Studies in intact animals, gastric glands, and isolated cells after short-term culture have shown expression of stimu...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00271.2005

    authors: Lambrecht NW,Yakubov I,Zer C,Sachs G

    更新日期:2006-03-13 00:00:00

  • Functional genomics of the dopaminergic system in hypertension.

    abstract::Abnormalities in dopamine production and receptor function have been described in human essential hypertension and rodent models of genetic hypertension. Under normal conditions, D(1)-like receptors (D(1) and D(5)) inhibit sodium transport in the kidney and intestine. However, in the Dahl salt-sensitive and spontaneou...

    journal_title:Physiological genomics

    pub_type: 杂志文章,评审

    doi:10.1152/physiolgenomics.00127.2004

    authors: Zeng C,Sanada H,Watanabe H,Eisner GM,Felder RA,Jose PA

    更新日期:2004-11-17 00:00:00

  • Novel slc22 transporter homologs in fly, worm, and human clarify the phylogeny of organic anion and cation transporters.

    abstract::Slc22 family organic anion and cation transporters (OATs, OCTs, and OCTNs) are transmembrane proteins expressed predominantly in kidney and liver. These proteins mediate the uptake or excretion of numerous physiologically (and pharmacologically) important compounds, and accordingly have been the focus of intensive stu...

    journal_title:Physiological genomics

    pub_type: 杂志文章,评审

    doi:10.1152/physiolgenomics.00014.2004

    authors: Eraly SA,Monte JC,Nigam SK

    更新日期:2004-06-17 00:00:00

  • Knockin mice with Leu9'Ser alpha4-nicotinic receptors: substantia nigra dopaminergic neurons are hypersensitive to agonist and lost postnatally.

    abstract::This study analyzes the electrophysiological cause and behavioral consequence of dopaminergic cell loss in a knockin mouse strain bearing hypersensitive nicotinic alpha4-receptor subunits ("L9'S mice"). Adult brains of L9'S mice show moderate loss of substantia nigra dopaminergic neurons and of striatal dopaminergic i...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00012.2004

    authors: Orb S,Wieacker J,Labarca C,Fonck C,Lester HA,Schwarz J

    更新日期:2004-08-11 00:00:00

  • Steroid sulfatase gene variation and DHEA responsiveness to resistance exercise in MERET.

    abstract::Genetic influences and endurance exercise have been shown to alter circulating concentrations of dehydroepiandrosterone (DHEA) and its sulfated conjugate, DHEAS. We hypothesized that acute resistance exercise (RE) and training (RET) would increase DHEA steroids, and the magnitude of the increase would be influenced by...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00097.2003

    authors: Riechman SE,Fabian TJ,Kroboth PD,Ferrell RE

    更新日期:2004-05-19 00:00:00

  • Salmonid genomes have a remarkably expanded akirin family, coexpressed with genes from conserved pathways governing skeletal muscle growth and catabolism.

    abstract::Metazoan akirin genes regulate innate immunity, myogenesis, and carcinogenesis. Invertebrates typically have one family member, while most tetrapod and teleost vertebrates have one to three. We demonstrate an expanded repertoire of eight family members in genomes of four salmonid fishes, owing to paralog preservation ...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00045.2010

    authors: Macqueen DJ,Kristjánsson BK,Johnston IA

    更新日期:2010-06-01 00:00:00

  • New quantitative trait loci for carotid atherosclerosis identified in an intercross derived from apolipoprotein E-deficient mouse strains.

    abstract::Carotid atherosclerosis is the primary cause of ischemic stroke. To identify genetic factors contributing to carotid atherosclerosis, we performed quantitative trait locus (QTL) analysis using female mice derived from an intercross between C57BL/6J (B6) and BALB/cJ (BALB) apolipoprotein E (Apoe(-/-)) mice. We started ...

    journal_title:Physiological genomics

    pub_type: 杂志文章

    doi:10.1152/physiolgenomics.00099.2012

    authors: Rowlan JS,Zhang Z,Wang Q,Fang Y,Shi W

    更新日期:2013-04-16 00:00:00