Abstract:
:Little is known regarding the graft response to transplantation injury. This study investigates the posttransplantation response of genes that are constitutively expressed in the heart. Constitutive heart and lymph node tissue-restricted gene expression was first analyzed with DNA microarrays. To demonstrate changes following transplantation in genes constitutively expressed in the heart, we performed vascularized murine heart transplants in allogeneic (BALB/c to B6), syngeneic (B6 to B6), and alymphoid (BALB/c-RAG2-/- to B6-RAG1-/-) experimental groups. Temporal induction of genes posttransplant relative to constitutive expression was evaluated with DNA microarrays. Dendrograms and self-organizing maps were generated to determine the dissimilarity between the experimental groups and to identify subsets of differentially expressed genes within the groups, respectively. Expression patterns of selected genes were confirmed by real-time PCR. Biological processes were assigned to genes induced posttransplant using the AnnBuilder package via the Gene Ontology Database. Post-transplant, a shift was noted in genes classified as defense, communication, and metabolism. Our results identify novel components of the graft response to transplantation injury and rejection.
journal_name
Physiol Genomicsjournal_title
Physiological genomicsauthors
Christopher K,Mueller TF,DeFina R,Liang Y,Zhang J,Gentleman R,Perkins DLdoi
10.1152/physiolgenomics.00139.2002subject
Has Abstractpub_date
2003-09-29 00:00:00pages
52-64issue
1eissn
1094-8341issn
1531-2267pii
15/1/52journal_volume
15pub_type
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