Abstract:
:Physiological, morphological, and transcriptional alterations elicited by ethynyl estradiol in the uteri of Sprague-Dawley rats and C57BL/6 mice were assessed using comparable study designs, microarray platforms, and analysis methods to identify conserved estrogen signaling networks. Comparative analysis identified 153 orthologous gene pairs that were positively correlated, suggesting conserved transcriptional targets important in uterine proliferation. Functional annotation for these responses were associated with angiogenesis, water and solute transport, cell cycle control, redox control, DNA replication, protein synthesis and transport, xenobiotic metabolism, cell-cell communication, energetics, and cholesterol and fatty acid regulation. The identification of conserved temporal expression patterns of these orthologs provides experimental support for the transfer of functional annotation from mouse orthologs to 44 previously unannotated rat expressed sequence tags based on their homology and co-expression patterns. The identification of comparable temporal phenotypic responses linked to related gene expression profiles demonstrates the ability of systematic comparative genomic assessments to elucidate important conserved mechanisms in rodent estrogen signaling during uterine proliferation.
journal_name
Physiol Genomicsjournal_title
Physiological genomicsauthors
Kwekel JC,Burgoon LD,Burt JW,Harkema JR,Zacharewski TRdoi
10.1152/physiolgenomics.00175.2005subject
Has Abstractpub_date
2005-11-17 00:00:00pages
327-42issue
3eissn
1094-8341issn
1531-2267pii
00175.2005journal_volume
23pub_type
杂志文章abstract::We investigated the expression and function of serum response factor (SRF) in endothelial-mesenchymal transition (EndMT) in glomerular endothelial cells (GEnCs) of diabetic nephropathy (DN). The expression of SRF, endothelial markers (VE-cadherin, CD31), and mesenchymal markers (α-SMA, FSP-1, fibronectin) was examined...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00082.2016
更新日期:2016-10-01 00:00:00
abstract::The genetic basis of susceptibility to pulmonary fibrosis is largely unknown. Initially, in this study, loci regulating the response of bleomycin-induced pulmonary fibrosis were mapped using a set of recombinant congenic strains bred from pulmonary fibrosis-resistant A/J and susceptible C57BL/6J (B6) mice. Linkage was...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00095.2005
更新日期:2005-09-21 00:00:00
abstract::A cDNA microarray resource has been developed with the goal of providing integrated functional genomics resources for cattle. The National Bovine Functional Genomics Consortium's (NBFGC) expressed sequence tag (EST) collection was established in 2001 to develop resources for functional genomics research. The NBFGC EST...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00094.2003
更新日期:2003-10-17 00:00:00
abstract::Septa from sheep hearts at 130 days gestation, term, and 14-day-old lambs were used to model the changes in gene expression patterns during the perinatal period using Agilent 15k ovine microarrays. We used Bioconductor for R to model five major patterns of coexpressed genes. Gene ontology and transcription factor anal...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00027.2015
更新日期:2015-09-01 00:00:00
abstract::In several species caloric restriction (CR) extends life span. In this paper we integrate data from studies on CR and other sources to articulate the hypothalamic deregulation hypothesis by which estrogen receptor-alpha (ER-α) signaling in the hypothalamus and limbic system affects life span under the stress of CR in ...
journal_title:Physiological genomics
pub_type: 杂志文章,评审
doi:10.1152/physiolgenomics.00073.2016
更新日期:2017-02-01 00:00:00
abstract::We previously reported that mice deficient in stearoyl-CoA desaturase-1 (Scd1) and maintained on a very low-fat (VLF) diet for 10 days developed severe loss of body weight, hypoglycemia, hypercholesterolemia, and many cholestasis-like phenotypes. To better understand the metabolic changes associated with these phenoty...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00139.2007
更新日期:2008-05-13 00:00:00
abstract::Cardiac microvascular endothelial cells (CMVECs) are the most numerous cells in the myocardium and orchestrate cardiogenesis during development, regulate adult cardiac function, and modulate pathophysiology of heart failure. It has been shown that the transcriptome of CMVECs differs from other endothelial cell types, ...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00002.2019
更新日期:2019-06-01 00:00:00
abstract::Teleost fish represent unique models to study the role of neuroestrogens because of the extremely high activity of brain aromatase (AroB; the product of cyp19a1b). Aromatase respectively converts androstenedione and testosterone to estrone and 17beta-estradiol (E2). Specific inhibition of aromatase activity by fadrozo...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00051.2009
更新日期:2009-08-07 00:00:00
abstract::Ductus arteriosus (DA) closure is initiated by oxygen rise postnatally and progresses in two, functional-to-permanent, stages. Here, using GeneChip Arrays in rats (normoxic and hyperoxic fetus, normoxic newborn), we examined whether oxygen alone duplicates the birth process in affecting DA genes. In addition, by compa...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00231.2005
更新日期:2006-04-13 00:00:00
abstract::High-frequency ultrasound biomicroscopy (UBM) has recently emerged as a high-resolution means of phenotyping genetically altered mice and has great potential to evaluate the cardiac morphology and hemodynamics of mouse mutants. However, there is no standard procedure of in vivo transthoracic cardiac imaging using UBM ...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00026.2004
更新日期:2004-07-08 00:00:00
abstract::We describe longitudinal measurements of functional residual capacity (FRC) in breathing mice using a clinical computed tomography (CT) scanner. Lungs of anesthetized mice from the A/J and C3H/HeJ strains were scanned over a 10-s period. Using a fixed threshold for CT density, we could accurately and reproducibly obta...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.2001.4.3.215
更新日期:2001-01-19 00:00:00
abstract::The liver of dairy cows is involved in signaling the current hepatic metabolic state to the brain via metabolites and nerval afferents to control and adjust feed intake. Feed deprivation may result in mobilization of body reserves favoring hepatic steatosis. While the overall metabolic changes are well characterized, ...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.90381.2008
更新日期:2009-04-10 00:00:00
abstract::Gene expression was measured during t10c12-CLA-induced body fat reduction in a polygenic obese line of mice. Adult mice (n = 185) were allotted to a 2 x 2 factorial experiment consisting of either nonobese (ICR-control) or obese (M16-selected) mice fed a 7% fat, purified diet containing either 1% linoleic acid (LA) or...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00244.2004
更新日期:2005-05-11 00:00:00
abstract::Large datasets from -omics studies need to be deeply investigated. The aim of this paper is to provide a new method (LEM method) for the search of transcriptome and metabolome connections. The heuristic algorithm here described extends the classical canonical correlation analysis (CCA) to a high number of variables (w...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00139.2012
更新日期:2013-07-15 00:00:00
abstract::Valve formation during embryonic heart development involves a complex interplay of regional specification, cell transformations, and remodeling events. While many studies have addressed the role of specific genes during this process, a global understanding of the genetic basis for the regional specification and develo...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00142.2009
更新日期:2010-02-04 00:00:00
abstract::Bone marrow mononuclear cells (BMMNCs) increase capillary density and reduce fibrosis in rodents after myocardial infarction, resulting in an overall improvement in left ventricular function. Little is known about the effectiveness of BMMNC therapy in hypertensive heart disease. In the current study, we show that deli...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00170.2011
更新日期:2012-10-02 00:00:00
abstract::Survival of near-freezing body temperatures and reduced blood flow during hibernation is likely the result of changes in the expression of specific genes. In this study, we described a comprehensive survey of mRNAs in the heart of the thirteen-lined ground squirrel (Spermophilus tridecemlineatus) before and during hib...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00076.2005
更新日期:2005-10-17 00:00:00
abstract::Mesodermal and epidermal precursor cells undergo phenotypic changes during differentiation to the smooth muscle cell (SMC) lineage that are relevant to pathophysiological processes in the adult. Molecular mechanisms that underlie lineage determination and terminal differentiation of this cell type have received much a...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00148.2004
更新日期:2004-11-17 00:00:00
abstract::Aside from abnormal angiogenesis, dual endothelin-1/VEGF signal peptide-activated receptor deficiency (DEspR(-/-)) results in aberrant neuroepithelium and neural tube differentiation, thus elucidating DEspR's role in neurogenesis. With the emerging importance of neurogenesis in adulthood, we tested the hypothesis that...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00044.2008
更新日期:2008-11-12 00:00:00
abstract::The gut microbiome plays a critical role in the onset and progression of obesity and the metabolic syndrome. However, it is not well documented whether the cecal vs. the fecal microbiome is more relevant when assessing their contributions to these diseases. Here, we amplified the V4 region of the 16S rRNA gene from ce...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00110.2017
更新日期:2018-05-01 00:00:00
abstract::Systemic lupus erythematosus (SLE) is an autoimmune disorder with immune-complex deposition that affects multiple organs. Previous studies have suggested the involvement of oxidative stress and apoptosis in SLE, but no clear link to etiology has been established. Here we show that mice deficient in a transcription fac...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00209.2003
更新日期:2004-08-11 00:00:00
abstract::To facilitate the development of assays for the discovery of pharmaceuticals capable of mimicking the effects of caloric restriction (CR) on life- and healthspan (CR mimetics), we evaluated the effectiveness of glucoregulatory and putative cancer chemopreventatives in reproducing the hepatic gene expression profile pr...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00069.2005
更新日期:2005-11-17 00:00:00
abstract::Expression patterns of monocarboxylate transporter 2 (MCT2) display mRNA diversity in a tissue-specific fashion. We cloned and characterized multiple mct2 5'-cDNA ends from the mouse and determined the structural organization of the mct2 gene. We found that transcription of this gene was initiated from five independen...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00192.2007
更新日期:2007-12-19 00:00:00
abstract::Myocardial infarction (MI) induced by acute coronary arterial occlusion is usually secondary to atherosclerotic plaque rupture. Dysregulated response of vascular smooth muscle cells (VSMCs) in atherosclerotic plaques may promote plaque rupture. Cadherins (CDHs) form adherens junctions and are known stabilizers of athe...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00042.2017
更新日期:2018-08-01 00:00:00
abstract::alpha-Sarcoglycan-deficient (Sgca-null) mice provide potential for elucidating the pathogenesis of limb girdle muscular dystrophy type 2D (LGMD 2D) as well as for studying the effectiveness of therapeutic strategies. Skeletal muscles of Sgca-null mice demonstrate an early onset of extensive fiber necrosis, degeneratio...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00311.2004
更新日期:2005-07-14 00:00:00
abstract::Higher fat and lower carbohydrate and amino acid oxidation are observed in women compared with men during endurance exercise. We hypothesized that the observed sex difference is due to estrogen and that menstrual cycle phase or supplementation of men with 17beta-estradiol (E(2)) would coordinately influence the mRNA c...
journal_title:Physiological genomics
pub_type: 杂志文章,随机对照试验
doi:10.1152/physiolgenomics.00115.2009
更新日期:2009-12-30 00:00:00
abstract::Peripheral blood cells are an accessible environment in which to visualize exercise-induced alterations in global gene expression patterns. We aimed to identify a peripheral blood mononuclear cell (PBMC) signature represented by alterations in gene expression, in response to a standardized endurance exercise training ...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00072.2014
更新日期:2015-02-01 00:00:00
abstract::An increased phospholamban (PLB)-to-sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA) ratio has been suggested to contribute to the slowing of relaxation in failing human ventricle. We have used an adenoviral vector carrying the sequence for PLB to increase this ratio in isolated adult rat ventricular myocytes, and w...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.1999.1.2.41
更新日期:1999-08-31 00:00:00
abstract::Morbidity and mortality associated with acute lung injury (ALI) and acute respiratory distress syndrome remain substantial. Although many candidate genes have been tested, a clear understanding of the pathogenesis is lacking, as is our ability to predict individual outcome. Because ALI is a complex disease, single gen...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.90392.2008
更新日期:2009-07-09 00:00:00
abstract::Usher syndrome (USH) is a neurosensory disorder affecting both hearing and vision in humans. Linkage studies of families of USH patients, studies in animals, and characterization of purified proteins have provided insight into the molecular mechanisms of hearing. To date, 11 USH proteins have been identified, and evid...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00135.2013
更新日期:2013-11-01 00:00:00