Abstract:
:Teleost fish represent unique models to study the role of neuroestrogens because of the extremely high activity of brain aromatase (AroB; the product of cyp19a1b). Aromatase respectively converts androstenedione and testosterone to estrone and 17beta-estradiol (E2). Specific inhibition of aromatase activity by fadrozole has been shown to impair estrogen production and influence neuroendocrine and reproductive functions in fish, amphibians, and rodents. However, very few studies have identified the global transcriptomic response to fadrozole-induced decline of estrogens in a physiological context. In our study, sexually mature prespawning female goldfish were exposed to fadrozole (50 mcirog/l) in March and April when goldfish have the highest AroB activity and maximal gonadal size. Fadrozole treatment significantly decreased serum E2 levels (4.7 times lower; P = 0.027) and depressed AroB mRNA expression threefold in both the telencephalon (P = 0.021) and the hypothalamus (P = 0.006). Microarray expression profiling of the telencephalon identified 98 differentially expressed genes after fadrozole treatment (q value <0.05). Some of these genes have shown previously to be estrogen responsive in either fish or other species, including rat, mouse, and human. Gene ontology analysis together with functional annotations revealed several regulatory themes for physiological estrogen action in fish brain that include the regulation of calcium signaling pathway and autoregulation of estrogen receptor action. Real-time PCR verified microarray data for decreased (activin-betaA) or increased (calmodulin, ornithine decarboxylase 1) mRNA expression. These data have implications for our understanding of estrogen actions in the adult vertebrate brain.
journal_name
Physiol Genomicsjournal_title
Physiological genomicsauthors
Zhang D,Popesku JT,Martyniuk CJ,Xiong H,Duarte-Guterman P,Yao L,Xia X,Trudeau VLdoi
10.1152/physiolgenomics.00051.2009subject
Has Abstractpub_date
2009-08-07 00:00:00pages
351-61issue
3eissn
1094-8341issn
1531-2267pii
00051.2009journal_volume
38pub_type
杂志文章abstract::Chromogranin A (CHGA) plays a catalytic role in formation of catecholamine storage vesicles and also serves as precursor to the peptide fragment catestatin, a catecholamine secretory inhibitor whose expression is diminished in the hypertensive individuals. We previously reported the hypertensive, hyperadrenergic pheno...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00086.2009
更新日期:2010-03-03 00:00:00
abstract::Functional proteomic strategies offer unique advantages over current molecular array approaches, as the epitopes identified can directly provide bioactive peptides for investigational and/or translational applications. The vascular endothelium is well suited to proteomic assessment by in vivo phage display, but extens...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00025.2006
更新日期:2006-08-16 00:00:00
abstract::In functional genomics, the high-throughput methods such as microarrays 1) allow analysis of the relationships between genes considering them as elements of a network and 2) lead to biological interpretations thanks to Gene Ontology. But up to now it has not been possible to find relationships between the functions an...
journal_title:Physiological genomics
pub_type: 杂志文章,meta分析
doi:10.1152/physiolgenomics.00008.2008
更新日期:2008-06-12 00:00:00
abstract::RNA viruses represent the predominant cause of many clinically relevant viral diseases in humans. Among several evolutionary advantages acquired by RNA viruses, the ability to usurp host cellular machinery and evade antiviral immune responses is imperative. During the past decade, RNA interference mechanisms, especial...
journal_title:Physiological genomics
pub_type: 杂志文章,评审
doi:10.1152/physiolgenomics.00112.2013
更新日期:2013-11-15 00:00:00
abstract::Peroxisome proliferator-activated receptor type gamma (PPARgamma) is a subgroup of the PPAR transcription factor family. Recent studies indicate that loss of PPARgamma is associated with the development of pulmonary hypertension (PH). We hypothesized that the endothelial dysfunction associated with PPARgamma inhibitio...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00094.2009
更新日期:2009-12-30 00:00:00
abstract::Myocardial infarction (MI) induced by acute coronary arterial occlusion is usually secondary to atherosclerotic plaque rupture. Dysregulated response of vascular smooth muscle cells (VSMCs) in atherosclerotic plaques may promote plaque rupture. Cadherins (CDHs) form adherens junctions and are known stabilizers of athe...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00042.2017
更新日期:2018-08-01 00:00:00
abstract::We previously reported that markers on rat chromosome 1 are genetically linked to blood pressure in an F(2) population derived from Dahl salt hypertension-sensitive (S) and Lewis (LEW) rats. Because there was evidence for more than one blood pressure quantitative trait locus (QTL) on chromosome 1, an initial congenic ...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.1999.1.3.119
更新日期:1999-11-11 00:00:00
abstract::Proteomics, analogous with genomics, is the analysis of the protein complement present in a cell, organ, or organism at any given time. While the genome provides information about the theoretical status of the cellular proteins, the proteome describes the actual content, which ultimately determines the phenotype. The ...
journal_title:Physiological genomics
pub_type: 杂志文章,评审
doi:10.1152/physiolgenomics.00282.2007
更新日期:2008-03-14 00:00:00
abstract::Immobilization, bed rest, or denervation leads to muscle disuse and subsequent skeletal muscle atrophy. Muscle atrophy can also occur as a component of various chronic diseases such as cancer, AIDS, sepsis, diabetes, and chronic heart failure or as a direct result of genetic muscle disorders. In addition to this atrop...
journal_title:Physiological genomics
pub_type: 杂志文章,评审
doi:10.1152/physiolgenomics.00043.2018
更新日期:2018-09-01 00:00:00
abstract::Epigenetic differences between male and female bovine blastocysts provide a plausible link between physiological and gene transcription differences observed between male and female embryos. The aim of this study was to examine sex-related epigenetic differences in bovine blastocysts produced in vitro. Oocytes were mat...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00234.2007
更新日期:2008-01-17 00:00:00
abstract::Besides neuronal plasticity, the neurotrophin brain-derived neurotrophic factor (BDNF) is also important in vascular function. The BDNF has been associated with angiogenesis through its specific receptor tropomyosin-related kinase B (TrkB). Additionally, Val66Met polymorphism decreases activity-induced BDNF. Since BDN...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00086.2015
更新日期:2016-02-01 00:00:00
abstract::Although increased vascular stiffness is more prominent in aging males than females, and males are more prone to vascular disease with aging, no study has investigated the genes potentially responsible for sex differences in vascular aging. We tested the hypothesis that the transcriptional adaptation to aging differs ...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00229.2006
更新日期:2007-04-24 00:00:00
abstract::We previously reported that mice deficient in stearoyl-CoA desaturase-1 (Scd1) and maintained on a very low-fat (VLF) diet for 10 days developed severe loss of body weight, hypoglycemia, hypercholesterolemia, and many cholestasis-like phenotypes. To better understand the metabolic changes associated with these phenoty...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00139.2007
更新日期:2008-05-13 00:00:00
abstract::The mammalian liver works to keep the body in a state of homeostasis and plays an important role in systemic acute phase response to infections. In this study we investigated the bovine hepatic acute phase response at the gene transcription level in dairy cows with experimentally Escherichia coli-induced mastitis. At ...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00084.2011
更新日期:2012-06-01 00:00:00
abstract::MicroRNAs (miRs) are small, noncoding RNAs that are emerging as crucial regulators of cardiac remodeling in left ventricular hypertrophy (LVH) and failure (LVF). However, there are no data on their role in right ventricular hypertrophy (RVH) and failure (RVF). This is a critical question given that the RV is uniquely ...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00163.2011
更新日期:2012-05-01 00:00:00
abstract::Although a great deal has been elucidated concerning the mechanisms regulating muscle differentiation, little is known about transcription factor-specific gene regulation. Our understanding of the genetic mechanisms regulating cell differentiation is quite limited. Much of what has been defined centers on regulatory s...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00149.2004
更新日期:2004-12-15 00:00:00
abstract::Bioaccumulative environmental estrogen, nonylphenol (NP; 4-nonylphenol), is widely used as a nonionic surfactant and can affect human health. Since genomes of Saccharomyces cerevisiae and higher eukaryotes share many structural and functional similarities, we investigated subcellular effects of NP on S. cerevisiae BY4...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00034.2017
更新日期:2017-10-01 00:00:00
abstract::The SMXA-5 recombinant inbred strain, which was established from nondiabetic parental SM/J and A/J mice, develops diabetic phenotypes such as impaired glucose tolerance. The combination of diabetogenic genes in the SM/J and A/J genomes impairs glucose tolerance in SMXA-5 mice. Using (SM/J x SMXA-5)F2 mice fed a high-f...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00027.2008
更新日期:2008-09-17 00:00:00
abstract::We previously generated a conditional floxed mouse line to study androgen action, in which exon 3 of the androgen receptor (AR) gene is flanked by loxP sites, with the neomycin resistance gene present in intron 3. Deletion of exon 3 in global AR knockout mice causes androgen insensitivity syndrome, characterized by ge...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00260.2007
更新日期:2008-03-14 00:00:00
abstract::Heart disease is the major cause of death in diabetes, a disorder characterized by chronic hyperglycemia and cardiovascular complications. Diabetic cardiomyopathy (DCM) is increasingly recognized as a major contributor to diastolic dysfunction and heart failure in diabetes, but its molecular basis has remained obscure...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00204.2006
更新日期:2007-02-12 00:00:00
abstract::We describe longitudinal measurements of functional residual capacity (FRC) in breathing mice using a clinical computed tomography (CT) scanner. Lungs of anesthetized mice from the A/J and C3H/HeJ strains were scanned over a 10-s period. Using a fixed threshold for CT density, we could accurately and reproducibly obta...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.2001.4.3.215
更新日期:2001-01-19 00:00:00
abstract::To further define genes that are differentially expressed during cysteine deprivation and to evaluate the roles of amino acid deprivation vs. oxidative stress in the response to cysteine deprivation, we assessed gene expression in human hepatoma cells cultured in complete or cysteine-deficient medium. Overall, C3A cel...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00263.2007
更新日期:2008-04-22 00:00:00
abstract::Several critical cell functions are influenced not only by internal cellular machinery but also by external mechanical and biochemical cues from the surrounding microenvironment. Slight changes to the microenvironment can result in dramatic changes to the cell's phenotype; for example, a change in the nutrients or pH ...
journal_title:Physiological genomics
pub_type: 杂志文章,评审
doi:10.1152/physiolgenomics.00170.2013
更新日期:2014-05-01 00:00:00
abstract::Experimental rat models of arthritis are extensively studied with a view to understand the genetic underpinnings of rheumatoid arthritis (RA). Genome scans using these models have led to the detection of arthritis regulatory quantitative trait loci (QTLs) on all but three chromosomes of the rat. Whereas some of the QT...
journal_title:Physiological genomics
pub_type: 杂志文章,评审
doi:10.1152/physiolgenomics.00034.2005
更新日期:2006-10-03 00:00:00
abstract::We in this study investigated the role of imatinib-upregulated lncRNA (IUR) in prostate carcinoma (PC). We observed that IUR was downregulated in PC, and its expression levels decreased with the increase of clinical stages. In PC tissues, microRNA (miR)-200 was positively, while ZEB1 was inversely correlated with IUR....
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00062.2019
更新日期:2019-11-01 00:00:00
abstract::The structural organization of the brain is important for normal brain function and is critical to understand in order to evaluate changes that occur during disease processes. Three-dimensional (3D) imaging of the mouse brain is necessary to appreciate the spatial context of structures within the brain. In addition, t...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00055.2012
更新日期:2012-08-01 00:00:00
abstract::Hypertension poses a significant challenge to vasculature homeostasis and stands as the most common cardiovascular disease in the world. Its effects are especially profound on endothelial cells that form the inner lining of the vasculature and are directly exposed to the effects of excess pressure. Here, we characteri...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00111.2017
更新日期:2018-02-01 00:00:00
abstract::Vasopressin is a peptide hormone that regulates renal water excretion in part through its actions on the collecting duct. The regulation occurs in part via control of transcription of genes coding for the water channels aquaporin-2 (Aqp2) and aquaporin-3 (Aqp3). To identify transcription factors expressed in collectin...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00148.2009
更新日期:2010-02-04 00:00:00
abstract::Growth hormone is one of few pharmacologic agents known to augment milk production in humans. We hypothesized that recombinant human GH (rhGH) increases the expression of cell proliferation and milk protein synthesis genes. Sequential milk and blood samples collected over four days were obtained from five normal lacta...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00079.2010
更新日期:2011-04-27 00:00:00
abstract::A novel coronavirus disease, COVID-19, has created a global pandemic in 2020, posing an enormous challenge to healthcare systems and affected communities. COVID-19 is caused by Severe Acute Respiratory Syndrome (SARS)-CoronaVirus-2 (CoV-2) that manifests as bronchitis, pneumonia, or a severe respiratory illness. SARS-...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00087.2020
更新日期:2020-12-04 00:00:00