Abstract:
:Mesodermal and epidermal precursor cells undergo phenotypic changes during differentiation to the smooth muscle cell (SMC) lineage that are relevant to pathophysiological processes in the adult. Molecular mechanisms that underlie lineage determination and terminal differentiation of this cell type have received much attention, but the genetic program that regulates these processes has not been fully defined. Study of SMC differentiation has been facilitated by development of the P19-derived A404 embryonal cell line, which differentiates toward this lineage in the presence of retinoic acid and allows selection for cells adopting a SMC fate through a differentiation-specific drug marker. We sought to define global alterations in gene expression by studying A404 cells during SMC differentiation with oligonucleotide microarray transcriptional profiling. Using an in situ 60-mer array platform with more than 20,000 mouse genes derived from the National Institute on Aging clone set, we identified 2,739 genes that were significantly upregulated after differentiation was completed (false-detection ratio <1). These genes encode numerous markers known to characterize differentiated SMC, as well as many unknown factors. We further characterized the sequential patterns of gene expression during the differentiation time course, particularly for known transcription factor families, providing new insights into the regulation of the differentiation process. Changes in genes associated with specific biological ontology-based pathways were evaluated, and temporal trends were identified for functional pathways. In addition to confirming the utility of the A404 model, our data provide a large-scale perspective of gene regulation during SMC differentiation.
journal_name
Physiol Genomicsjournal_title
Physiological genomicsauthors
Spin JM,Nallamshetty S,Tabibiazar R,Ashley EA,King JY,Chen M,Tsao PS,Quertermous Tdoi
10.1152/physiolgenomics.00148.2004subject
Has Abstractpub_date
2004-11-17 00:00:00pages
292-302issue
3eissn
1094-8341issn
1531-2267pii
00148.2004journal_volume
19pub_type
杂志文章abstract::Besides neuronal plasticity, the neurotrophin brain-derived neurotrophic factor (BDNF) is also important in vascular function. The BDNF has been associated with angiogenesis through its specific receptor tropomyosin-related kinase B (TrkB). Additionally, Val66Met polymorphism decreases activity-induced BDNF. Since BDN...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00086.2015
更新日期:2016-02-01 00:00:00
abstract::Hypertension is a condition with major cardiovascular and renal complications, affecting nearly a billion patients worldwide. Few validated gene targets are available for pharmacological intervention, so there is a need to identify new biological pathways regulating blood pressure and containing novel targets for trea...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00010.2010
更新日期:2010-09-01 00:00:00
abstract::The structural organization of the brain is important for normal brain function and is critical to understand in order to evaluate changes that occur during disease processes. Three-dimensional (3D) imaging of the mouse brain is necessary to appreciate the spatial context of structures within the brain. In addition, t...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00055.2012
更新日期:2012-08-01 00:00:00
abstract::The renin-angiotensin system (RAS), known for its roles in cardiovascular, metabolic, and developmental regulation, is present in both the circulation and in many individual tissues throughout the body. Substantial evidence supports the existence of a brain RAS, though quantification and localization of brain renin ha...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00208.2010
更新日期:2011-03-29 00:00:00
abstract::A microarray study of the effect of senescence in mice on gene expression in muscle has been published recently. The present analysis was done to evaluate the extent to which the age-related differences in gene expression in murine muscle are also evident in human muscle. RNA extracted from muscle of young (21-24 yr) ...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.2001.5.2.67
更新日期:2001-03-08 00:00:00
abstract::The objectives of this study were to examine systemic insulin response to a glucose tolerance test (GTT) and transcript abundance of genes of the insulin signaling pathway in skeletal muscle, during both dietary restriction and re-alimentation-induced compensatory growth. Holstein Friesian bulls were blocked to one of...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00002.2015
更新日期:2015-08-01 00:00:00
abstract::Psoriasis is a chronic and common human skin disorder currently with no cure. Psoriatic skin displays inflammatory, raised, and scaly lesions with widely aberrant gene expression. Recent studies have revealed critical roles that microRNAs play as a class of posttranscriptional gene regulator in skin development and sk...
journal_title:Physiological genomics
pub_type: 杂志文章,评审
doi:10.1152/physiolgenomics.00157.2013
更新日期:2014-02-15 00:00:00
abstract::The mammalian liver works to keep the body in a state of homeostasis and plays an important role in systemic acute phase response to infections. In this study we investigated the bovine hepatic acute phase response at the gene transcription level in dairy cows with experimentally Escherichia coli-induced mastitis. At ...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00084.2011
更新日期:2012-06-01 00:00:00
abstract::Hypoxia is a widely occurring condition experienced by diverse organisms under numerous physiological and disease conditions. To probe the molecular mechanisms underlying hypoxia responses and tolerance, we performed a genome-wide screen to identify mutants with enhanced hypoxia tolerance in the model eukaryote, the y...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00232.2010
更新日期:2011-07-27 00:00:00
abstract::Since the discovery of the microbiome in humans, it has been studied in many mammalian species. Different microbiological communities with variable richness and diversity have been found among these species in distinct areas of the reproductive tract. Human studies have shown that the composition of the microbiome is ...
journal_title:Physiological genomics
pub_type: 杂志文章,评审
doi:10.1152/physiolgenomics.00045.2019
更新日期:2019-08-01 00:00:00
abstract::microRNAs (miRNAs) are short noncoding RNAs that regulate gene expression through posttranscriptional repression of target genes. miRNAs exert a fundamental level of control over many developmental processes, but their role in the differentiation and development of skeletal muscle from myogenic progenitor cells in hum...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00037.2014
更新日期:2015-03-01 00:00:00
abstract::Ductus arteriosus (DA) closure is initiated by oxygen rise postnatally and progresses in two, functional-to-permanent, stages. Here, using GeneChip Arrays in rats (normoxic and hyperoxic fetus, normoxic newborn), we examined whether oxygen alone duplicates the birth process in affecting DA genes. In addition, by compa...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00231.2005
更新日期:2006-04-13 00:00:00
abstract::This study analyzes the electrophysiological cause and behavioral consequence of dopaminergic cell loss in a knockin mouse strain bearing hypersensitive nicotinic alpha4-receptor subunits ("L9'S mice"). Adult brains of L9'S mice show moderate loss of substantia nigra dopaminergic neurons and of striatal dopaminergic i...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00012.2004
更新日期:2004-08-11 00:00:00
abstract::Epistasis effects (gene interactions) have been increasingly recognized as important genetic factors underlying complex traits. The existence of a large number of single nucleotide polymorphisms (SNPs) provides opportunities and challenges to screen DNA variations affecting complex traits using a candidate gene analys...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00096.2006
更新日期:2006-12-13 00:00:00
abstract::This study was the first to explore the potential role of the myostatin (GDF8) pathway in relation to muscle strength and estimated muscle cross-sectional area in humans using linkage analysis with a candidate gene approach. In young male sibs (n = 329) 11 polymorphic markers in or near 10 candidate genes from the myo...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00224.2003
更新日期:2004-05-19 00:00:00
abstract::Slc22 family organic anion and cation transporters (OATs, OCTs, and OCTNs) are transmembrane proteins expressed predominantly in kidney and liver. These proteins mediate the uptake or excretion of numerous physiologically (and pharmacologically) important compounds, and accordingly have been the focus of intensive stu...
journal_title:Physiological genomics
pub_type: 杂志文章,评审
doi:10.1152/physiolgenomics.00014.2004
更新日期:2004-06-17 00:00:00
abstract::Sulfate plays an essential role during growth, development and cellular metabolism. In this study, we characterized the function and structure of the zebrafish (Danio rerio) Na+-sulfate cotransporter 1 (NaS1) cDNA and gene (slc13a1). Zebrafish NaS1 encodes a protein of 583 amino acids with 13 putative transmembrane do...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.90234.2008
更新日期:2008-08-15 00:00:00
abstract::Hypoxia is an important ecological, evolutionary, and biomedical stressor. While physiological acclimatization of mammals to hypoxia is well studied, the variation in gene expression that underlies acclimatization is not well studied. We acclimatized inbred mice for 32 days to hypoxic conditions that simulated altitud...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00075.2009
更新日期:2010-05-01 00:00:00
abstract::Teleost fish represent unique models to study the role of neuroestrogens because of the extremely high activity of brain aromatase (AroB; the product of cyp19a1b). Aromatase respectively converts androstenedione and testosterone to estrone and 17beta-estradiol (E2). Specific inhibition of aromatase activity by fadrozo...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00051.2009
更新日期:2009-08-07 00:00:00
abstract::Large datasets from -omics studies need to be deeply investigated. The aim of this paper is to provide a new method (LEM method) for the search of transcriptome and metabolome connections. The heuristic algorithm here described extends the classical canonical correlation analysis (CCA) to a high number of variables (w...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00139.2012
更新日期:2013-07-15 00:00:00
abstract::Chronic kidney disease (CKD) is associated with increased cardiovascular morbidity and mortality. Previous studies indicated an impairment of ischemia-induced angiogenesis in skeletal muscle of rats with CKD. We performed a systematic comparison of early gene expression in response to ischemia in rats with or without ...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00124.2016
更新日期:2017-04-01 00:00:00
abstract::Vasopressin is a peptide hormone that regulates renal water excretion in part through its actions on the collecting duct. The regulation occurs in part via control of transcription of genes coding for the water channels aquaporin-2 (Aqp2) and aquaporin-3 (Aqp3). To identify transcription factors expressed in collectin...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00148.2009
更新日期:2010-02-04 00:00:00
abstract::To examine differences in cytokine profiles that may confer tolerance/susceptibility to bovine African trypanosomiasis, N'Dama (trypanotolerant, n = 8) and Boran (trypanosusceptible, n = 8) cattle were experimentally challenged with Trypanosoma congolense. Blood samples were collected over a 34-day period, and RNA was...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00100.2006
更新日期:2006-12-13 00:00:00
abstract::The purpose of this study was to determine the influence of age, sex, and strength training (ST) on large-scale gene expression patterns in vastus lateralis muscle biopsies using high-density cDNA microarrays and quantitative PCR. Muscle samples from sedentary young (20-30 yr) and older (65-75 yr) men and women (5 per...
journal_title:Physiological genomics
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1152/physiolgenomics.00028.2002
更新日期:2002-09-03 00:00:00
abstract::Aortic banding in the rat has become a popular method to induce left ventricular (LV) hypertrophy and heart failure. However, because of often extensive intrathoracic adhesions and inflammatory cell infiltrates resulting from the traditional surgical approach, an uncomplicated second thoracic incision for genetic mani...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00035.2001
更新日期:2002-01-01 00:00:00
abstract::Although a great deal has been elucidated concerning the mechanisms regulating muscle differentiation, little is known about transcription factor-specific gene regulation. Our understanding of the genetic mechanisms regulating cell differentiation is quite limited. Much of what has been defined centers on regulatory s...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00149.2004
更新日期:2004-12-15 00:00:00
abstract::Aside from abnormal angiogenesis, dual endothelin-1/VEGF signal peptide-activated receptor deficiency (DEspR(-/-)) results in aberrant neuroepithelium and neural tube differentiation, thus elucidating DEspR's role in neurogenesis. With the emerging importance of neurogenesis in adulthood, we tested the hypothesis that...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00044.2008
更新日期:2008-11-12 00:00:00
abstract::Creatine monohydrate (CrM) supplementation has been shown to increase fat-free mass and muscle power output possibly via cell swelling. Little is known about the cellular response to CrM. We investigated the effect of short-term CrM supplementation on global and targeted mRNA expression and protein content in human sk...
journal_title:Physiological genomics
pub_type: 杂志文章,随机对照试验
doi:10.1152/physiolgenomics.00157.2007
更新日期:2008-01-17 00:00:00
abstract::We previously reported that markers on rat chromosome 1 are genetically linked to blood pressure in an F(2) population derived from Dahl salt hypertension-sensitive (S) and Lewis (LEW) rats. Because there was evidence for more than one blood pressure quantitative trait locus (QTL) on chromosome 1, an initial congenic ...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.1999.1.3.119
更新日期:1999-11-11 00:00:00
abstract::Adenosine deaminase acting on RNA (ADAR) in Drosophila and mammals has recently become the target of numerous investigations. It is now clear that this protein has a number of functions in the nervous system. Indeed, the mutation of ADAR in Drosophila (dADAR) results in many pathological and physiological changes, suc...
journal_title:Physiological genomics
pub_type: 杂志文章
doi:10.1152/physiolgenomics.00093.2003
更新日期:2005-01-20 00:00:00