Abstract：
:NMDA receptor activation leads to elevated Ca2+ in cultured rat cortical and retinal ganglion cell neurons. If excessive, this Ca2+ response is associated with delayed neurotoxicity. We used dantrolene and ionomycin to test if the Ca2+ response to NMDA was due to mobilization of intracellular Ca2+ stores rather than merely to Ca2+ influx. In the presence of EGTA, ionomycin resulted in release and subsequent depletion of intracellular Ca2+ stores. Henceforth, despite normal extracellular Ca2+, NMDA elicited only about half of its former Ca2+ response. Similarly, when dantrolene was used to block Ca2+ release from intracellular stores, we observed > 50% smaller NMDA-evoked Ca2+ responses. These results quite surprisingly indicate that at least half the Ca2+ response to NMDA is due to release of intracellular Ca2+, a process triggered by influx of extracellular Ca2+. Dantrolene also protected neurons from NMDA receptor-mediated neurotoxicity. Release of intracellular Ca2+ may therefore be a necessary step in the cascade leading to neuronal damage induced by excessive NMDA receptor stimulation and may be amenable to pharmacological intervention.
journal_name
Brain Resjournal_title
Brain researchauthors
Lei SZ,Zhang D,Abele AE,Lipton SAdoi
10.1016/0006-8993(92)90183-asubject
Has Abstractpub_date
1992-12-11 00:00:00pages
196-202issue
1-2eissn
0006-8993issn
1872-6240pii
0006-8993(92)90183-Ajournal_volume
598pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:The following data were obtained from microelectrode exploration of rabbit auditory cortex (AC). (1) Location of AC--Major portions of the rabbit's right hemisphere were stereotaxically mapped for auditory responsive and non-responsive regions. Auditory responsive regions were found to lie behind the rhinal sulcus, ex...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)91194-x
更新日期：1981-06-15 00:00:00
abstract：:To examine the effect of development on rabbit brain insulin and insulin-like growth factor (IGF) receptor expression, we characterized and quantitated receptor mRNAs by Northern blot analysis and affinity-labeled ligand bound receptors by SDS-PAGE and autoradiography. At various stages of development ranging from 23 ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)91361-u
更新日期：1993-03-05 00:00:00
abstract：:Co-activation of group I metabotropic glutamate (mGlu) receptors and adenosine receptors resulted in an augmented cyclic AMP response in primary cultures of rat striatal neurones. L-glutamate and the selective group I agonist, (S)-dihydroxyphenylglycine (S-DHPG) evoked concentration-dependent potentiations of cyclic A...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(98)00094-8
更新日期：1998-04-27 00:00:00
abstract：:Lifelong bilingualism is associated with the delayed diagnosis of dementia, suggesting bilingual experience is relevant to brain health in aging. While the effects of bilingualism on cognitive functions across the lifespan are well documented, less is known about the neural substrates underlying differential behaviour...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2015.02.034
更新日期：2015-07-01 00:00:00
abstract：:We reported earlier that overnight change in explicit memory is positively related to the change in sleep spindle activity (between a control and a learning night). However, it remained unclear whether this effect was restricted to good memory performers and whether a general association of sleep spindles and a "sleep...
journal_title：Brain research
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.brainres.2007.10.106
更新日期：2008-01-29 00:00:00
abstract：:We examined whether mild brain hypothermia during pretreatment with sublethal 2-min ischemia affected the tolerance to subsequent lethal 5-min ischemia. The neuronal densities in the hippocampal CA1 sector of gerbils preconditioned at mild brain hypothermia (32% of normal) were significantly lower than those in gerbil...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(96)01139-0
更新日期：1997-01-23 00:00:00
abstract：:The relationship between seizure propensity and the vascular anatomy of the anterior cerebral arteries, or stroke induced by unilateral ligation of the common carotid artery, was investigated utilizing well differentiated colonies of seizure-prone (SP) and non-seizure-prone (NSP) gerbils. Twenty SP and 20 NSP gerbils ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(82)90867-8
更新日期：1982-02-25 00:00:00
abstract：:After traumatic brain injury, extracellular K(+) in brain can dramatically increase. We studied the effects of increased K(+) on the isolated pressurized rat middle cerebral artery (MCA). MCAs (200-250 microm OD) were isolated, cannulated with glass micropipettes, and pressurized. K(+) was increased in the extralumina...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)02793-1
更新日期：2000-10-13 00:00:00
abstract：:In the present study, uptake of glutamine by rat cerebellar granule cells, a predominantly glutamatergic nerve cell population, has been investigated. Glutamine is taken up by granule cells via at least three transport systems, A, ASC and L. The L-type low affinity system (K(m) = 2.6 mM) is the major transport system ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00139-x
更新日期：1997-05-16 00:00:00
abstract：:Inhibition of nitric oxide (NO) synthesis has been found to produce learning deficits in spatial tasks. Recent studies also suggest a regulatory effect of endogenous NO on hippocampal serotonin (5-HT) release and have shown that NO-synthase (NOS) inhibitors increased extracellular levels of serotonin (5-HT) in the rat...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)03987-2
更新日期：2003-02-14 00:00:00
abstract：:The cognitive reserve hypothesis proposes that the brain actively attempts to cope with age-related changes by using pre-existing cognitive networks (neural reserve) or enlisting compensatory processes (neural compensation). In a context of visual selective attention, the current study compared task-related activation...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2012.02.061
更新日期：2012-05-15 00:00:00
abstract：:The recently identified neuropeptide QRFP(26) is predominantly expressed in the hypothalamus and was suggested to play a role in the regulation of food intake following the observation of an acute orexigenic effect after central administration in mice. QRFP(26) exerts its effect via GPR103 and a newly identified recep...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.08.055
更新日期：2006-11-13 00:00:00
abstract：:Neuronal cells in primary culture have been demonstrated to possess specific insulin receptors (Boyd et al., J. Biol. Chem., 260 (1985) 15880-15884). Incubation of these cultures with insulin causes a dose-dependent inhibition of maprotiline-sensitive [3H]norepinephrine uptake. Maximum inhibition of 95% of maprotiline...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91242-4
更新日期：1986-11-19 00:00:00
abstract：:Intra- and interhemispheric connections between the anterior ectosylvian visual area (AEV) and other visual cortical areas including the lateral suprasylvian (LSS) were examined in the cat using the retrograde double-label fluorescence technique. The areal and laminar distributions of labeled neurons were mapped follo...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)90189-1
更新日期：1985-11-18 00:00:00
abstract：:Horseradish peroxidase (HRP) was used as a retrograde tracer to identify the distribution pattern of labeled cells in the inferior olivary nucleus (IO) of Tupaia. Crystallized HRP was implanted into dorsal (DPML) and ventral (VPML) divisions of the paramedian lobule (PML) and, following appropriate survival times, the...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90433-5
更新日期：1984-07-09 00:00:00
abstract：:FRAT1 was originally characterized as a protein frequently rearranged in advanced T cell lymphoma, which inhibits GSK-3-mediated phosphorylation of beta-catenin and positively regulates the Wnt signaling pathway. FRAT1 has been identified as a proto-oncogene involved in tumorigenesis. Previous studies have shown that ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.01.037
更新日期：2010-03-12 00:00:00
abstract：:Effects of protein kinase C (PKC) activation on the function of the GABA/benzodiazepine receptor-chloride complex were analyzed by quantitative autoradiography using [3H]muscimol, [3H]flunitrazepam and [35S]TBPS in rat brain slices. The density of [3H]muscimol binding was highest in cerebellar granular layers and high...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)02127-7
更新日期：1999-12-11 00:00:00
abstract：:Amacrine cells are axonless interneurons of the vertebrate retina. Over the past few years many neuroactive peptides have been localized to these cells. We have previously demonstrated that a neuropeptide and a classical transmitter are colocalized in some amacrine cells. In this report, we show that two neuroactive p...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)90850-9
更新日期：1985-10-14 00:00:00
abstract：:The localization of prostaglandin endoperoxide synthase in monkey brain was investigated by the immunoperoxidase method using the monoclonal antibody (PES-7) raised against the enzyme purified from bovine seminal vesicle. The frozen sections with 30-microns thickness were employed after the brain was fixed with perfus...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)91043-z
更新日期：1991-03-08 00:00:00
abstract：:The Snell dwarf mouse (dw/dw) shows significantly decreased somatostatin levels in the hypothalamus whereas more or less increased somatostatin concentrations are observed in any central extrahypothalamic sites studied. These opposite results in hypothalamus and extrahypothalamic areas may be linked to the apparently ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)91337-x
更新日期：1985-02-25 00:00:00
abstract：:Male Wistar rats were anaesthetized, injected intracisternally (i.c.) with saline or colchicine and were decapitated at various time intervals. Trunk blood was collected for the determination of immunoreactive adrenocorticotropic hormone (ACTHi) by radioimmunoassay (RIA) and of corticosterone by a fluorometric assay. ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)91517-x
更新日期：1988-03-01 00:00:00
abstract：:Mechanical hyperalgesia is an early symptom of diabetic neuropathy. To evaluate the mechanisms underlying this symptom, it was studied and compared in rat models of systemic and local hyperglycemia. Systemic hyperglycemia was induced by a single injection of streptozotocin (STZ, 50 mg/kg). Local hyperglycemia either i...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)03828-3
更新日期：2003-01-17 00:00:00
abstract：:Activity produced by direct microelectrophoretic application of glutamate onto 19 convergent neurones in trigeminal nucleus caudalis, was strongly depressed during and after the application of heterotopic noxious conditioning stimuli: noxious heat (52 degrees C) applied to the tail, noxious pinches applied to the tail...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90695-4
更新日期：1984-10-29 00:00:00
abstract：:Opiates and cannabinoids are among the most widely consumed habit-forming drugs in humans. Several studies have demonstrated the existence of interactions between both kind of drugs in a variety of effects and experimental models. The present study has been focused to determine whether perinatal delta9-tetrahydrocanna...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(98)00766-5
更新日期：1998-10-05 00:00:00
abstract：:Smoking is considered the leading cause of preventable death in the United States, but studies in animals suggest that nicotine is only weakly reinforcing. The maintenance of a dangerous habit by a weakly reinforcing agent has been the topic of some dispute. Using a two-lever "choice" self-administration procedure dev...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)03215-2
更新日期：2002-01-04 00:00:00
abstract：:The distribution of calcitonin gene-related peptide (CGRP)-immunoreactive (IR) fibers and cell bodies was studied in the spinal cord and the medulla oblongata of the grey monkey (Macaca fascicularis) using peroxidase-antiperoxidase (PAP) immunohistochemistry. At all levels of the spinal cord many CGRP-IR motoneurons a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)90787-v
更新日期：1991-09-06 00:00:00
abstract：:The roles of spinal N-methyl-d-aspartic acid receptor 2B (NR2B) subunit in central sensitization of chronic visceral pain were investigated. A rat model with irritable bowel syndrome (IBS) was established by colorectal distention (CRD) on post-natal days 8-14. Responses of the external oblique muscle of the abdomen to...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2013.10.008
更新日期：2014-01-13 00:00:00
abstract：:The effect of different dose, mode and duration of estradiol administration was examined in the different brain catecholaminergic areas in ovariectomized (OVX) female rats. We determined changes in mRNA levels of tyrosine hydroxylase (TH), rate-limiting enzyme in catecholamine (CA) biosynthesis of GTP cyclohydrolase I...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2004.04.002
更新日期：2004-07-23 00:00:00
abstract：:At present, the ability of polyunsaturated fatty acids (PUFAs) to regulate individual glutamate transporter subtypes is poorly understood and very little information exists on the mechanism(s) by which PUFAs achieve their effects on the transport process. Here we investigate the effect of cis-4,7,10,13,16,19-docosahex...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2005.01.008
更新日期：2005-03-10 00:00:00
abstract：:Interpretation of the cellular and molecular pathogenic basis of post-minimal traumatic brain injury is a significant clinical and scientific problem, especially due to the high prevalence of motor vehicle--and other accidents. Pathogenetic brain mechanisms following traumatic impact are usually investigated by using ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.10.032
更新日期：2007-01-26 00:00:00
