Abstract：
BACKGROUND:Numerous data support the possible role of myeloblastin/proteinase 3 (PR3) in growth and differentiation of neutrophil granulocytes and certain monocyte subtypes. However, whether PR3 is expressed in non-myeloid cells remains a matter of debate even though recent studies clearly demonstrated its expression in endothelium, kidney epithelial cells and epithelial tumor cell lines. METHODS:To survey PR3 transcript presence in human tissues, we analyzed different human tissues by dot blot and northern blot using a cloned PR3-cDNA probe. To examine the physiological function of PR3 expression in non-myeloid cells, we constructed different recombinant retroviral vectors containing human PR3-cDNA variants and expressed them in tubular epithelial cells (TEC). Using an MTT-based proliferation assay, we determined the proliferation rate of PR3-transduced kidney cells. RESULTS:The resulting expression pattern clearly indicated that PR3 transcripts are not only present in tissues known to harbor hematopoietic cells, but surprisingly PR3 was highly expressed in fetal organs including the kidney. The proliferation assay revealed that the growth rate of TEC transduced with native PR3 was significantly enhanced relative to non-transduced TEC. CONCLUSIONS:The results supported our theory that PR3 can act as a growth factor in non-hematopoietic cells, analogous to its role in hematopoietic cells. The cells, recombinant vectors and methods described here serve as a basis to investigate PR3 function in cellular differentiation and proliferation, as well as its role in autoimmune diseases.
journal_name
J Nephroljournal_title
Journal of nephrologyauthors
Relle M,Mayet WJ,Strand D,Brenner W,Galle PR,Schwarting Asubject
Has Abstractpub_date
2003-11-01 00:00:00pages
831-40issue
6eissn
1121-8428issn
1724-6059journal_volume
16pub_type
杂志文章abstract：BACKGROUND:Chronic kidney disease (CKD) is an established risk factor for cardiovascular disease (CVD). In addition, patients with renal disease are exposed to a myriad of risk factors that increase their risk even further. The treatment of risk factors in these patients is paramount to reducing cardiovascular risk and...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：BACKGROUND:Adiponectin, a novel adipocytokine with antiinflammatory and antiatherosclerosis properties, has been found to be an inverse predictor of cardiovascular outcomes among uremic patients on hemodialysis. However, its role in uremic patients on continuous ambulatory peritoneal dialysis (CAPD) remains unclear. Th...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2008-09-01 00:00:00
abstract：:During Fabry disease, progressive glycosphingolipid deposition in the kidney causes gradual deterioration of renal function with proteinuria, uremia and hypertension. This results in end-stage renal disease (ESRD) which is one of the leading causes of morbidity and premature mortality in affected patients. Given the e...
journal_title：Journal of nephrology
pub_type： 杂志文章,评审
doi：10.5301/jn.5000214
更新日期：2013-07-01 00:00:00
abstract：:Chronic renal failure (CRF) is associated with a 20-fold increased risk of cardiovascular death, two principal mechanisms being: sudden, arrhythmic death associated with left ventricular hypertrophy, and ischaemic heart disease, associated with accelerated atherosclerosis. In recent years, the vascular endothelium has...
journal_title：Journal of nephrology
pub_type： 杂志文章,评审
doi：
更新日期：2000-03-01 00:00:00
abstract：BACKGROUND:This study investigated whether modifications of placental expression of endothelin-1 (ET-1), nitric oxide synthase (NOS) and tissue factor (TF) are associated with abnormal Doppler waveforms. METHODS:Fourteen pre-term severe preeclamptic (PE) women with fetal growth restriction (FGR) and 14 normal preg nan...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2003-09-01 00:00:00
abstract：:That systemic hypertension is involved in the progression of human renal disease is mostly suggested by the way anti-hypertensive treatment affects the course of the disease. Clinical evidence has been obtained from observational studies as well as from studies of dietary protein restriction. In addition, several tria...
journal_title：Journal of nephrology
pub_type： 杂志文章,评审
doi：
更新日期：2000-05-01 00:00:00
abstract：BACKGROUND:The therapeutic effects of immunosuppressive agents such as cyclosporin A, mycophenolate mofetil, rituximab and sirolimus on primary focal segmental glomerulosclerosis (FSGS) remain controversial. The present study was intended to review and analyze the treatment results of immunosuppressive agents for prima...
journal_title：Journal of nephrology
pub_type： 杂志文章,评审
doi：10.5301/jn.5000092
更新日期：2012-09-01 00:00:00
abstract：:The attitude of 19th century (and even of 20th century) scholars toward medieval and, particularly, Anglo-Saxon medicine has been of severe criticism. According to them it was filled with superstition and stupidities. However, in these last fifty years research has proved that, compared with the Continent, Anglo-Saxon...
journal_title：Journal of nephrology
pub_type： 历史文章,杂志文章
doi：
更新日期：2009-11-01 00:00:00
abstract：BACKGROUND:Stenoses of vascular accesses are leading factors limiting access survival. Besides physical examination, screening of access stenoses is based mainly on the "dysfunction hypothesis", which states that progressive stenosis causes graft dysfunction, such as decreased flow. We tested whether Doppler ultrasonog...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
abstract：:We report the first documented case of X-linked chronic granulomatous disease (CGD) in association with IgA nephropathy in a 22 year old male with multiple soft tissue abscesses. The case highlights the potential role of staphylococcal infections in the pathogenesis of IgA nephropathy and suggests the hypothesis that ...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
abstract：BACKGROUND AND METHODS:Smoking is associated with hyperparathyroidism in the elderly general population and nicotine, the main component of tobacco smoke, stimulates PTH release in experimental models. Although smoking is a persisting problem in patients with end-stage renal disease (ESRD), the association between smok...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：10.5301/JN.2011.7740
更新日期：2012-01-01 00:00:00
abstract：:The management of rapidly progressive glomerulonephritis has been transformed over the past thirty years. It has become one of the few forms of glomerulonephritis that can be effectively treated, and today overall renal survival is as high as 70%. Effective management of patients with RPGN requires prompt and accurate...
journal_title：Journal of nephrology
pub_type： 杂志文章,评审
doi：
更新日期：1999-07-01 00:00:00
abstract：:To determine the clinical impact of conversion from C0 to C2 Neoral monitoring, we conducted a 6-month prospective study in 62 stable renal transplant recipients. Neoral was given alone (19%), with steroids (31%), combined with azathioprine (Aza) or mycophenolate mofetil (MMF) (50%). C0 and C2 target ranges were, resp...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2004-03-01 00:00:00
abstract：:The collecting duct is responsible for the final secretion or reabsorption of protons and bicarbonate, respectively. Approximately 2% of the total filtered bicarbonate is reabsorbed in this segment. At least two different types of cells are involved in the transport of protons and bicarbonate, namely, type A and B int...
journal_title：Journal of nephrology
pub_type： 杂志文章,评审
doi：
更新日期：2002-03-01 00:00:00
abstract：:Patients with type 2 diabetes run a high risk for progressive renal function loss. Many interventions have been tested to reduce the risk, but we are nowadays still confronted with a high unmet need. To improve on this unmet need, we will have to change the current strategies in drug discovery, clinical trials and cli...
journal_title：Journal of nephrology
pub_type： 杂志文章,评审
doi：10.1007/s40620-020-00820-2
更新日期：2020-12-01 00:00:00
abstract：:Primary renal non-Hodgkin's lymphoma is very rare in childhood. A six-year-old boy presented with bilateral non-obstructive multinodular nephromegaly and renal failure. Percutaneous needle biopsy showed large-cell lymphoma. The patient was started on chemotherapy. A right nephrectomy was done when systemic hypertensio...
journal_title：Journal of nephrology
pub_type： 杂志文章,评审
doi：
更新日期：2002-09-01 00:00:00
abstract：:A 59 year-old woman showed rapidly progressive glomerulonephritis during immunotherapy for metastatic renal cell carcinoma. She received unilateral nephrectomy and cytotoxic T lymphocyte (CTL) therapy for the treatment of retroperitoneal lymph node metastasis of renal cell carcinoma. With CTL therapy, her retroperiton...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2005-07-01 00:00:00
abstract：:IgM secreting myelomas or lymphomas, including Waldenström macroglobulinemia, are associated with a varied spectrum of renal pathology, including intracapillary hyaline deposits, cryoglobulin, membranoproliferative glomerulonephritis, amyloid, monoclonal immunoglobulin deposition disease, cast nephropathy, and lymphom...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：10.1007/s40620-018-0544-9
更新日期：2018-12-01 00:00:00
abstract：BACKGROUND:Different mitogens are involved in the pathogenesis of kidney damage after subtotal nephrectomy. One of them, TGF-beta, controls mesangial cell proliferation and interstitial fibrosclerosis. The transduction of the TGF-beta signal is controlled by intracellular signalling molecules such as Ras G monomeric pr...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2002-09-01 00:00:00
abstract：:Modern nephrology developed in Poland mostly from the internal medicine department chaired in Warsaw by the great internist prof. Witold Orłowski (1874-1966). Three of his pupils Tadeusz Orłowski, Jan Roguski and Jakub Penson independently established the foundations of clinical nephrology and renal replacement therap...
journal_title：Journal of nephrology
pub_type： 历史文章,杂志文章
doi：
更新日期：2006-05-01 00:00:00
abstract：BACKGROUND:The G-1082A polymorphism of the interleukin-10 (IL-10) gene has been associated with modified gene expression and the progression of primary IgA nephropathy (IgAN). In the present study, we evaluated its influence on recurrent IgAN after renal transplantation. METHODS:We studied 103 patients who suffered fr...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2008-11-01 00:00:00
abstract：:Clinical immunology laboratories play an essential role in diagnosis and monitoring of systemic lupus erythematosus (SLE). To obtain the best results in terms of diagnostic performance and clinical usefulness, the following recommendations should be fulfilled: Indirect immunofluorescence on Hep-2 cells remains the met...
journal_title：Journal of nephrology
pub_type： 杂志文章,评审
doi：
更新日期：2002-11-01 00:00:00
abstract：BACKGROUND:Lipid peroxidation (LPO) plays a role in glomerulonephritis pathogenesis. The role of LPO in IgA nephropathy (IgAN) and the effect of prostaglandin E1 on it, is not determined. METHODS:Levels of malondialdehyde (MDA), superoxide dismutase (SOD) and vitamin E (vitE) were measured in 42 patients with IgAN and...
journal_title：Journal of nephrology
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：2005-05-01 00:00:00
abstract：:Cyclosporine A has been empirically used for more than 12 years in the treatment of idiopathic nephrotic syndrome, in both children and adults. There is consistent evidence, from both experimental and human studies, that the highly lipophilic cyclosporine molecule diminishes or abolishes proteinuria by two differing m...
journal_title：Journal of nephrology
pub_type： 杂志文章,评审
doi：
更新日期：1997-01-01 00:00:00
abstract：BACKGROUND:In biopsy-proven idiopathic nephrotic syndrome (INS), immunoglobulin M (IgM) and C1q are occasionally deposited in the mesangium. In pediatric nephrology, the significance of mesangial IgM or C1q deposits is controversial, based on previous reports. The aim of this study was to explore the clinical significa...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：10.5301/jn.5000133
更新日期：2013-03-01 00:00:00
abstract：:The role of the kidney in controlling and maintaining plasma potassium levels in the normal range requires the presence and activity of renal potassium channels, and their importance has been highlighted in patients with Bartter syndrome harboring mutations in the ROMK (Kir1.1, KCJN1) channel and hyperkalemia. However...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2010-01-01 00:00:00
abstract：BACKGROUND:The ongoing necessity for systemic heparinization is a well-known disadvantage of continuous renal replacement therapies (CRRT), and alternative methods of anticoagulation may be required. Our aim was to evaluate, in patients with a high risk of bleeding, the possibility of an acceptable filter life with non...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2003-07-01 00:00:00
abstract：BACKGROUND:To report clinical symptoms and outcome of systemic paraphenylene diamine (PPD) intoxication. METHODS:Our study was retrospective. It was conducted over 6 yrs (1994-2000) in the medical intensive care unit (ICU) of a university hospital and it concerned 19 patients hospitalized for systemic PPD intoxication...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2005-05-01 00:00:00
abstract：:The partial correction of anemia and the normalization of phosphate and blood pressure are the mainstay of treatment of patients with chronic kidney disease (CKD). Available anti-hypertensive drugs, erythropoiesis stimulating agents (ESAs) and iron supplements have resolved quite satisfactorily the goal of controlling...
journal_title：Journal of nephrology
pub_type： 杂志文章,评审
doi：10.1007/s40620-017-0421-y
更新日期：2017-12-01 00:00:00
abstract：BACKGROUND:Proteinuria results from increased glomerular permeability and is associated with retraction and effacement of the highly specialized interdigitating podocyte foot processes. In podocytes (glomerular epithelial cells [GECs]), p130Cas localizes diffusely to the cytoplasm and connects focal adhesion proteins a...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：10.5301/jn.5000261
更新日期：2013-09-01 00:00:00
