Abstract：
BACKGROUND:Proteinuria results from increased glomerular permeability and is associated with retraction and effacement of the highly specialized interdigitating podocyte foot processes. In podocytes (glomerular epithelial cells [GECs]), p130Cas localizes diffusely to the cytoplasm and connects focal adhesion proteins and kinases to the glomerular basement membrane and adapter proteins of slit diaphragm insertion sites. METHODS:To investigate whether high-glucose (HG) and advanced glycosylation end products (AGEs) induce quantitative and distributional changes of podocyte p130Cas protein, a docking protein connecting F-actin fibers to the glomerular basement membrane and adapter proteins, we cultured rat GECs (r-GECs) and mouse GECs (m-GECs) under (i) normal glucose (5 mM = control), (ii) HG (30 mM), (iii) AGE-added or (iv) HG plus AGE-added conditions. We also prepared streptozotocin-induced diabetic renal tissues. RESULTS:We found that p130Cas stainings were located in peripheral cytoplasm of r-GECs and m-GECs as dots in linear alignment, partially colocalized with actin-binding proteins such as synaptopodin and alpha-actinin, and locally connected to the ends of actin filaments. In diabetic conditions, the intensities of p130Cas stainings were diminished in more pathological HG plus AGE-added condition of r-GECs and m-GECs and in chronic diabetic renal tissues. p130Cas protein was decreased significantly by HG, AGE and HG plus AGE in both cells compared with normal glucose or osmotic control conditions. p130Cas mRNA expression levels were also suppressed similarly in diabetic conditions. CONCLUSION:We suggest that diabetic conditions modulate the quantitative and distributional changes of podocyte p130Cas and therefore affect the filtration function of podocytes.
journal_name
J Nephroljournal_title
Journal of nephrologyauthors
Ha TS,Choi JY,Park HY,Han GDdoi
10.5301/jn.5000261subject
Has Abstractpub_date
2013-09-01 00:00:00pages
870-6issue
5eissn
1121-8428issn
1724-6059pii
1D792E19-F14E-4DDE-A558-BE330C5EBF8Fjournal_volume
26pub_type
杂志文章abstract：BACKGROUND:Severe hyperhomocysteinemia is common in hemodialysis patients, who also present a dramatic increase in plasma concentrations of sulfate, one of the main products of methionine and cysteine catabolism. The aim of this study was to verify the relationship between high plasma sulfate levels and cysteine or hom...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：BACKGROUND:Serum adiponectin is inversely linked to obesity, impaired glucose homeostasis, dyslipidemia and hypertension and has been suggested as a possible marker of cardiovascular (CV) disease in the general population. However, its role in chronic kidney disease and following renal transplantation is not well estab...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2010-01-01 00:00:00
abstract：BACKGROUND:Oxidative stress plays a crucial role in chronic complications of diabetes such as diabetic nephropathy (DN), the main cause of renal failure. In diabetes, whether peroxynitrite (ONOO(-)), generated from inducible nitric oxide synthase (iNOS) induced by tumor necrosis factor alpha (TNF-alpha), plays a primar...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2009-11-01 00:00:00
abstract：BACKGROUND:In the general population and in hemodialysis patients epicardial adipose tissue (EAT) has been associated with increased mortality and cardiovascular events. Weight loss and lipid lowering therapies reduced EAT in the general population. It is unknown whether sevelamer, a phosphate (Pi) binder that lowers c...
journal_title：Journal of nephrology
pub_type： 杂志文章,随机对照试验
doi：10.1007/s40620-016-0310-9
更新日期：2016-10-01 00:00:00
abstract：:The management of rapidly progressive glomerulonephritis has been transformed over the past thirty years. It has become one of the few forms of glomerulonephritis that can be effectively treated, and today overall renal survival is as high as 70%. Effective management of patients with RPGN requires prompt and accurate...
journal_title：Journal of nephrology
pub_type： 杂志文章,评审
doi：
更新日期：1999-07-01 00:00:00
abstract：:A 17-year-old boy was admitted to the hospital twice in a year for 2 episodes of hemolytic uremic syndrome (HUS). During these 2 HUS episodes he had diarrhea, decreased serum complement, decreased total protein and decreased serum albumin concentrations. We suggest that protein-losing enteropathy and hypocomplementemi...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2007-03-01 00:00:00
abstract：:Modern nephrology developed in Poland mostly from the internal medicine department chaired in Warsaw by the great internist prof. Witold Orłowski (1874-1966). Three of his pupils Tadeusz Orłowski, Jan Roguski and Jakub Penson independently established the foundations of clinical nephrology and renal replacement therap...
journal_title：Journal of nephrology
pub_type： 历史文章,杂志文章
doi：
更新日期：2006-05-01 00:00:00
abstract：:Some patients with essential hypertension manifest increased urinary albumin excretion (UAE). Hypertensive patients with microalbuminuria manifest abnormal circadian variation of blood pressure, increased serum levels of LDL-cholesterol and lipoprotein(a), a greater rise of serum insulin in response to an oral glucose...
journal_title：Journal of nephrology
pub_type： 杂志文章,评审
doi：
更新日期：1997-07-01 00:00:00
abstract：:Given the alarming predictions of the potentially devastating future epidemic of end-stage renal disease (ESRD), further knowledge is still urgently needed on this topic. The real dimensions of the problem are still unclear. It is difficult to compare Europe and the USA because different criteria are used in data coll...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2005-03-01 00:00:00
abstract：:This review focuses on a series of risk factors involved in the pathogenesis of cardiovascular complications in patients undergoing Regular Dialysis Treatment (RDT). Many of them are not modifiable by any pharmacological strategies or dialysis therapy, others - such as phosphate overload - can be corrected successfull...
journal_title：Journal of nephrology
pub_type： 杂志文章,评审
doi：
更新日期：2006-01-01 00:00:00
abstract：BACKGROUND:Non-heart-beating donors suffer a period of warm ischaemia (WI) injury, resulting in higher rates of delayed graft function compared with heart-beating donors. Endothelin is believed to play a pathophysiological role in the intense vasospasm associated with WI. This study aimed to investigate renal function ...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：OBJECTIVE:It is uncertain whether increasing 25-hydroxyvitamin D (25-D) levels in chronic kidney disease (CKD) patients above those recommended by current guidelines result in progressive amelioration of secondary hyperparathyroidism. Our objective was to identify a potential therapeutic 25-D target which optimally low...
journal_title：Journal of nephrology
pub_type： 杂志文章,多中心研究
doi：10.1007/s40620-015-0186-0
更新日期：2016-02-01 00:00:00
abstract：:Recently, two independent teams detected presumed hepatitis agents, which were designated HGV and hepatitis GB virus C; they represent a new genus in the family Flaviviridae. The most accurate way to assess the epidemiology of GBV-C/HGV infection remains the combination of RT-PCR and anti-GBV-C/HGV E2 techniques, to d...
journal_title：Journal of nephrology
pub_type： 杂志文章,评审
doi：
更新日期：1999-05-01 00:00:00
abstract：BACKGROUND:The relationship between malnutrition in hemodialysis (HD) patients with a chronic inflammatory condition and the expression levels of leukocyte integrins and their adhesiveness to fibronectin was investigated. METHODS:Subjective global assessment, albumin and body mass index were used as nutritional marker...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2010-03-01 00:00:00
abstract：:Hypertension is prevalent and remains an important risk factor in elderly and very elderly. Randomized controlled outcome trials have shown benefit of antihypertensive treatment in patients with systolic diastolic hypertension and in patients with isolated systolic hypertension, aged 60 years and over. More recently b...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2009-09-01 00:00:00
abstract：:The relevance of the Salerno Medical School is examined through the contributions of Magister Maurus, Alfano I, Giovanni Plateario, Giovanni Afflacio, Bartolomeo and Urso of Calabria. An unpublished treatise on De Urinis in middlelatin and Italian volgare is introduced here for the first time by discussing the lapis o...
journal_title：Journal of nephrology
pub_type： 历史文章,杂志文章
doi：
更新日期：2004-03-01 00:00:00
abstract：:The most common cause of liver disease worldwide is now non-alcoholic fatty liver disease (NAFLD). NAFLD refers to a spectrum of disease ranging from steatosis to non-alcoholic steatohepatitis, causing cirrhosis, and ultimately hepatocellular carcinoma. However, the impact of NAFLD is not limited to the liver. NAFLD h...
journal_title：Journal of nephrology
pub_type： 杂志文章,评审
doi：10.1007/s40620-020-00751-y
更新日期：2020-05-21 00:00:00
abstract：INTRODUCTION:We measured serum creatinine concentrations in 17 male athletes of the Italian national rugby team. METHODS:Blood was obtained before the start of training and during the competitive season. Serum creatinine level was measured by Jaffé reaction at 4 time points during the season, with a formal measure of ...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：10.5301/jn.5000094
更新日期：2012-11-01 00:00:00
abstract：BACKGROUND:Previous studies have shown that bleeding times have positive predictive values of only 5% for perioperative bleeding in unselected populations. Nevertheless, performing bleeding times prior to all renal biopsies is common in nephrology practice. METHODS:We report complications of 112 renal biopsies done at...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
abstract：BACKGROUND:In older patients with chronic kidney disease (CKD), the risk of progression to end stage renal disease and cardiovascular death both differ compared to younger patients. This likely reflects differences in case mix and co-morbid burdens. We sought to establish the extent to which age itself is an independen...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：10.1007/s40620-018-0529-8
更新日期：2018-12-01 00:00:00
abstract：:Although several in vitro studies and clinical observations suggest that ACE-inhibitors (ACE-I) are a promising treatment for IgA nephropathy (IgAN), meta-analysis of published data is not yet conclusive. Therefore, a European double-blinded, prospective, randomized therapeutic trial was designed to evaluate ACE-I tre...
journal_title：Journal of nephrology
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：
更新日期：2001-11-01 00:00:00
abstract：BACKGROUND:Lipid peroxidation (LPO) plays a role in glomerulonephritis pathogenesis. The role of LPO in IgA nephropathy (IgAN) and the effect of prostaglandin E1 on it, is not determined. METHODS:Levels of malondialdehyde (MDA), superoxide dismutase (SOD) and vitamin E (vitE) were measured in 42 patients with IgAN and...
journal_title：Journal of nephrology
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：2005-05-01 00:00:00
abstract：BACKGROUND:An increased number of major histocompatibility complex (MHC) class II-positive cells (OX-6+ cells) were observed in the glomerulus and periglomerular interstitium during the course of anti-glomerular basement membrane (anti-GBM) crescentic glomerulonephritis (GN) in WKY rats. This study aimed to demonstrate...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2007-05-01 00:00:00
abstract：INTRODUCTION:We analyzed renal histologic and immunohistologic findings in children with nephrotic syndrome (NS) who did (n=5) or did not (n=17) develop cyclosporine A (CyA) nephropathy despite appropriately low serum CyA concentrations being maintained over 2 years. METHODS:To discriminate embryonic-type from mature ...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：10.5301/JN.2010.5793
更新日期：2011-07-01 00:00:00
abstract：:That systemic hypertension is involved in the progression of human renal disease is mostly suggested by the way anti-hypertensive treatment affects the course of the disease. Clinical evidence has been obtained from observational studies as well as from studies of dietary protein restriction. In addition, several tria...
journal_title：Journal of nephrology
pub_type： 杂志文章,评审
doi：
更新日期：2000-05-01 00:00:00
abstract：BACKGROUND:Drug-related problems (DRPs) are common in chronic kidney disease (CKD) patients. We developed a 2-step consultation including a clinical pharmacist (CP) session and a nephrologist conventional care consultation to explore the feasibility of a pilot drug-oriented disease management program in controlling iat...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：10.5301/jn.5000063
更新日期：2012-09-01 00:00:00
abstract：:Page kidney was first described in animal experiments in 1939. In the 1950s and 1960s the human counterpart became evident. In this review we examine the modest literature on this rare but important renal/urological complication, summarize the clinical features, and discuss the best approach to diagnosis and managemen...
journal_title：Journal of nephrology
pub_type： 杂志文章,评审
doi：
更新日期：2003-05-01 00:00:00
abstract：BACKGROUND AND AIMS:It has been well documented that gene and DNA alterations occur frequently in benign primary parathyroid adenomas as well as in parathyroid glands with secondary hyperplasia. However, it has not been shown whether a correlation exists between somatic DNA aberrations and clinical data. METHODS:We an...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2007-03-01 00:00:00
abstract：BACKGROUND:Chronic renal failure (CRF) is associated with a tendency to atherosclerosis due to the enhanced oxidative stress and insufficient antioxidant enzyme activities such as superoxide dismutase (SOD), catalase (CAT) and paraoxonase (PON 1), together with abnormalities in lipid parameters. We determined the in vi...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2002-03-01 00:00:00
abstract：INTRODUCTION:Post-transplant malignancies are among the most important complications in organ transplantation. Hemangioblastoma (HB) is especially prevalent in the cerebellum. CASE REPORT:A 20-year-old male who first started dialysis therapy, and then underwent kidney transplantation from a living-relative donor. Five...
journal_title：Journal of nephrology
pub_type： 杂志文章
doi：
更新日期：2005-11-01 00:00:00
