Abstract:
:Candidal adherence to mucosal surfaces is considered as the first step in the pathogenesis of oral candidiasis. We examined the effect of antifungal polyenes, amphotericin B, nystatin and natamycin, at sublethal and minimum inhibitory concentrations (MICs) on the adherence of Candida albicans and Candida glabrata to HeLa cervical carcinoma and HSC-3 oral squamous cell carcinoma cells. A total of six oral Candida isolates were used throughout the study. Two Candida strains, C. albicans (44990) and C. glabrata (MYA-275) were obtained from ATCC. Four Candida strains, C. albicans 19 and 24 and C. glabrata 15 and 21, were isolated from patients with documented Candida-associated denture stomatitis. Cells were either incubated with Candida in the presence of the drug, or pre-incubated with yeasts and exposed subsequently to the drug. In the drug-free controls, the mean number of C. albicans yeasts associated with HeLa cells obtained from all experiments (130.1+/-10.1 yeasts/mm(2)) was significantly greater than that for HSC-3 cells (114.7+/-10.1 yeasts/mm(2); P<0.025). For C. glabrata, the mean adherence to HeLa and HSC-3 cells was 84.4+/-5.5 and 84.4+/-3.3 yeasts/mm(2), respectively, and these values were not statistically different (P>0.4). Candidal adherence was significantly reduced when the tested polyenes were present during the "adherence phase". The obtained values were significantly different from the controls, except for the effect of nystatin at the MIC on the adherence of C. glabrata strain MYA-275 to HeLa cells (P<0.375). Amphotericin B had the highest effect against both Candida species, reducing adherence by approximately 50 and approximately 60%, at the MIC and sublethal concentrations, respectively. The susceptibility of cell-associated Candida to polyenes was decreased markedly and the treatment did not result in significant detachment of adherent yeasts. The reduction in adherence was between 2 and 10%, when compared to the drug-free controls. These findings suggest that sub-therapeutic levels of polyenes that are likely to persist in the oral cavity following topical treatment may modulate candidal colonization when present during the "adherence phase".
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Dorocka-Bobkowska B,Konopka K,Düzgüneş Ndoi
10.1016/s0003-9969(03)00174-2subject
Has Abstractpub_date
2003-12-01 00:00:00pages
805-14issue
12eissn
0003-9969issn
1879-1506pii
S0003996903001742journal_volume
48pub_type
杂志文章abstract:OBJECTIVE AND DESIGN:Autopsies of the submandibular gland, the vestibular folds and the soft palate from 65-87 old humans were examined to record the immunohistochemical expression of MUC1 and the simple mucin-type antigens Tn and Sialyl-Tn. RESULTS:(1) The serous acini in the submucosal glands from the larynx and the...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.07.007
更新日期:2010-11-01 00:00:00
abstract:UNLABELLED:Tooth enamel is formed by ameloblasts, which are derived from the epithelial cells of the enamel organ. OBJECTIVE:The purpose of this study was to grow human ameloblast-like epithelial cells in culture. DESIGN:Human fetal tooth organs were isolated, and the cells were separated by digestion in collagenase/...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2004.12.008
更新日期:2005-08-01 00:00:00
abstract::Various fruit juices with relatively low pH are known to have erosive effects on human tooth enamel in a reasonably short time. Honey, also with a relatively low pH, could do the same, but scanning electron microscopy showed no erosion of enamel by honey over a period of 30 min, neither did Knoop microhardness tests s...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(94)90110-4
更新日期:1994-02-01 00:00:00
abstract:OBJECTIVES:The aim of the present study was to identify the anticancer effects and the mechanisms of action of shikonin and its analogue isobutyrylshikonin in oral squamous carcinoma cells. DESIGNS:The cytotoxic effects of isobutyrylshikonin and shikonin in Ca9-22 and SCC-25 cells were analyzed using 3-(4,5-dimethylth...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2020.104774
更新日期:2020-08-01 00:00:00
abstract::Specific, well-characterized antisera to the extracellular matrix glycoprotein tenascin were used. Immunoreactivity was detected in association with dentinal tubules; it was particularly prominent in the tooth crown, and was stronger within matrix than within predentine. In contrast, there was no anti-fibronectin stai...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(91)90080-e
更新日期:1991-01-01 00:00:00
abstract::The major gamma-carboxyglutamic acid-containing protein of rabbit cortical bone isolated and purified from near-neutral (pH 7.5) EDTA-extracts by DEAE-cellulose and Sephadex G 75 column chromatography had a molecular weight of about 5600 based on integral amino-acid composition; this was confirmed by high-performance ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(84)90149-3
更新日期:1984-01-01 00:00:00
abstract:OBJECTIVE:Currently there is still a debate about whether peritubular dentine (PTD) is non-collageneous or collageneous tissue. The chemical composition and structure of human PTD and intertubular dentine (ITD) was re-visited in this study. DESIGN:The dentine tubular region including ITD and PTD prepared from human th...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2011.09.008
更新日期:2012-04-01 00:00:00
abstract::The Trk family, a group of high-affinity neurotrophin receptors, is divided into three subtypes, TrkA, TrkB and TrkC. These were originally found in neural elements, and are involved in neural development, maintenance and survival. Recent studies have shown that non-neural cells in vitro also express mRNA encoding som...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(97)00030-7
更新日期:1997-06-01 00:00:00
abstract::Hinokitiol, a natural iron-chelating agent, is known to have diverse biological and pharmacological activities in various cell types. However, the effect of hinokitiol on dental pulp cells has not yet been reported. In this study, hinokitiol increases hypoxia-inducible factor-1α (HIF-1α) protein levels and vascular en...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2013.10.009
更新日期:2014-02-01 00:00:00
abstract::Calcium and protein kinase C may be directly involved in exocytosis. However, in the rat submandibular gland, cAMP-mediated events appear to be required for mucin secretion. Calcium may be involved, but a direct signal-transduction role for calcium and protein kinase C in regulating such secretion has yet to be establ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(89)90075-7
更新日期:1989-01-01 00:00:00
abstract::The expression and distribution of inducible nitric oxide synthase (iNOS) was examined in 12 samples of human temporomandibular joint (TMJ) with internal derangement (ID) and four control specimens. In the diseased joints, strong or definite iNOS reactivity was expressed in synovial lining and endothelial cells; weake...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(00)00086-8
更新日期:2001-01-01 00:00:00
abstract:OBJECTIVE:Syndecan-1 plays an important role in cell proliferation in dental papilla during tooth development. This study aimed to clarify the transcription mechanisms that regulate syndecan-1 gene expression in dental papilla. DESIGN:We analysed genomic conservation and putative transcriptional factor binding sites o...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2011.10.017
更新日期:2012-05-01 00:00:00
abstract::The localization of cathepsin L and rat salivary cystatin-3 (RSC-3) in rat osteoclasts (rat femoral and alveolar bones) treated with or without E-64 (control) was examined immunocytochemically. In osteoclasts pretreated with E-64, immunoreactivity for cathepsin L was very weak extracellularly compared to that in the c...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(97)00003-4
更新日期:1997-04-01 00:00:00
abstract::Normal function of the lateral pterygoid muscle is not well understood. The principal aim of this study was to determine whether there is a progressive decrease in lateral pterygoid activity as the condyle moves posteriorly and superiorly during the return phase of a contralateral or protrusive jaw movement, as would ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(00)00011-x
更新日期:2000-06-01 00:00:00
abstract::Platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine; PAF) is a biologically active lipid, like the prostaglandins, which mediates allergic and inflammatory reactions. Aggregation of washed rabbit platelets was induced by a lipid prepared from inflamed gingiva. The mobility of the active lipid wa...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(89)90044-7
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND:External apical root resorption is a pathologic consequence of orthodontic tooth movement. Cementum and dentin are removed from the root surface while active force is present. OBJECTIVE:The aim of this study was to identify and quantify extracellular matrix proteins, dentin matrix protein 1 (DMP1), dentin p...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2006.08.018
更新日期:2007-03-01 00:00:00
abstract::The first indication of cartilage formation was detected on day 14.5 of pregnancy in close connection to the ossifying mandible. A separated blastema was never observed. Thus the findings support the concept that the condylar cartilage develops from already differentiated cells, not from primary undifferentiated mesen...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(95)00105-0
更新日期:1996-01-01 00:00:00
abstract:OBJECTIVE:Biofilms are complex, multi-species bacterial communities that colonize the oral cavity in the form of plaque and are known to cause dental caries and periodontal disease. Present study demonstrated the potential of three selected medicinal plants against isolated and identified dental biofilm forming strains...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2017.05.011
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVE:The aim of this study is to evaluate the performance of MALDI-TOF mass spectrometry in identifying bacteria isolated in the oral cavity known to be of probiotic interest. DESIGN:We evaluated Bruker MALDI Biotyper for the identification of 92 clinical oral isolates of probiotic interest (31 Streptococcus sali...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2020.104983
更新日期:2021-01-01 00:00:00
abstract::Salivary proteins and glycoproteins that participate in the formation of 2-h in vivo enamel pellicle were determined utilizing polyacrylamide gel electrophoresis [sodium dodecyl sulphate (SDS)-PAGE and anionic PAGE]/Western transfer analyses, and specific radiolabelling/SDS-PAGE fluorography. The sensitivity of these ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(89)90070-8
更新日期:1989-01-01 00:00:00
abstract:OBJECTIVE:To explore the gene mutation in an incontinentia pigmenti (IP) patient with syndromic tooth agenesis. METHODS:Long-range polymerase chain reaction (PCR) and Sanger sequencing were used to detect inhibitor of nuclear factor kappa-B kinase subunit gamma (IKBKG) mutation in the IP patient. We used the nuclear f...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2019.03.013
更新日期:2019-05-01 00:00:00
abstract::The ability of cadmium (Cd) to induce the synthesis of metallothionein (MT) in the developing teeth of the rat was investigated. Rats were given daily intraperitoneal injections of cadmium chloride (1.5 mg Cd/kg) for 7 days. The induction of MT synthesis in incisor teeth after Cd treatment was investigated immunohisto...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(98)00088-0
更新日期:1999-01-01 00:00:00
abstract:OBJECTIVES:Previous studies have suggested that the vasculature in the dental pulp and periodontal tissues plays an important role in producing the eruptive force in continuously erupting incisors. The aim of the present study was to investigate the effects of local injections of vasoactive drugs on regional blood flow...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2005.11.001
更新日期:2006-06-01 00:00:00
abstract:OBJECTIVE:Secondary cartilage develops from osteochondral progenitor cells. Hypertrophic chondrocytes in secondary cartilage increase within a very short time and then ossify rapidly. In the present study, we investigated the sequential development process of osteochondral progenitor cells, and the morphology and size ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2017.12.027
更新日期:2018-05-01 00:00:00
abstract:OBJECTIVE:This study aimed to evaluate the effect of fluoridated dentifrice (FD) and mouthwash (FM) under different treatment regimens on root caries (RC) inhibition. METHODS:Dual-species biofilms formed by Streptococcus mutans and Lactobacillus casei were grown on the surface of bovine root dentine slabs which were e...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2015.07.018
更新日期:2015-10-01 00:00:00
abstract:OBJECTIVES:This study evaluated the level and mechanism of apoptosis in human gingival fibroblasts (HGF) and murine fibroblasts (NIH/3T3) treated with a titanium tetrafluoride (TiF4) varnish compared those treated with a sodium fluoride (NaF) varnish. METHODS:Cells were treated with a TiF4, NaF (both 2.45%F) or placeb...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2018.10.039
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVE:Interleukin-1 (IL-1) is a proinflammatory cytokine that is highly elevated in response to bacterial biofilms and is a potential risk factor for periodontal diseases. IL-1 gene polymorphisms have been associated with the IL-1 level. The aim of this study was to investigate if IL-1 gene cluster polymorphisms ar...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.10.001
更新日期:2011-03-01 00:00:00
abstract:OBJECTIVE:The complex movement of the temporomandibular joint (TMJ) disc during mastication is controlled in large part by the disc's attachments to the surrounding tissues. This study seeks to address the lack of available quantitative data characterizing the extracellular matrix composition of the discal attachments ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2011.10.004
更新日期:2012-06-01 00:00:00
abstract::Mandibles were cultured in the presence of dibutyryl cyclic AMP (1 mM) and theophylline (1 mM) and in control medium. Controls differentiated normally but the timing of events differed from that in ovo and feathers did not form. With elevated intracellular cyclic-AMP levels, membrane bone did not form in the earlier s...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(86)90057-9
更新日期:1986-01-01 00:00:00
abstract:OBJECTIVE:The effect of experimental jaw-muscle pain and stress on masticatory muscle activity in TMD-patients has been discussed. Furthermore, associations between TMD and deep bite patients have been studied. Accordingly in the present study, comparison of EMG responses at rest, maximal clenching, during evoked pain ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2013.07.003
更新日期:2013-10-01 00:00:00