Abstract:
OBJECTIVE:The aim of this study is to evaluate the performance of MALDI-TOF mass spectrometry in identifying bacteria isolated in the oral cavity known to be of probiotic interest. DESIGN:We evaluated Bruker MALDI Biotyper for the identification of 92 clinical oral isolates of probiotic interest (31 Streptococcus salivarius and 61 Lactobacillus spp.) by comparing direct colony method with on-plate formic acid extraction. Isolates were previously identified by use of biochemical methods and molecular biology. RESULTS:Using the manufacturer's suggested genus and species level cutoff scores, the direct colony method identified 42 (45.7%) isolates at the genus level and 35 (38%) at the species level while the on-plate extraction method correctly identified 90 (97.8%) isolates at the genus level and 82 (89.1%) at the species level. The difference between the two methods was statistically significant at the genus and species levels (P ≤ 0.0001). After dividing the isolates into two subgroups, the analysis was repeated. The direct colony method identified correctly all isolates of Streptococcus salivarius at the species level. In contrast, the direct colony method allowed the identification of only 11 (18%) lactobacilli at the genus level and 4 (6.6%) at the species level. The on-plate extraction method was statistically (P ≤ 0.0001) more efficient since 59 (96.7%) lactobacilli were identified at the genus level and 51 (83.6%) at the species level. CONCLUSIONS:MALDI Biotyper can efficiently identify Streptococcus salivarius regardless of the preparative method but on-plate extraction is superior to direct colony method for the identification of lactobacilli.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Jussiaux F,Miot-Sertier C,Nguyen-Lopez D,Badet C,Samot Jdoi
10.1016/j.archoralbio.2020.104983subject
Has Abstractpub_date
2021-01-01 00:00:00pages
104983eissn
0003-9969issn
1879-1506pii
S0003-9969(20)30361-7journal_volume
121pub_type
杂志文章abstract::Degradation of phenylalanine and leucine by resting cells of Eubacterium brachy ATCC 33089 were studied under strict anaerobic conditions. The effects of metronidazole and air on the metabolism were also studied. The organism principally produced phenylpropionate and isocaproate from phenylalanine and leucine, respect...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(94)90080-9
更新日期:1994-11-01 00:00:00
abstract::Normal keratinocytes from epidermis and from buccal mucosa underwent dissimilar stages of differentiation in the same culture medium and responded differently to changes in the composition of the medium. Manifestations of these variations were examined in terms of the expression at the mRNA level (as measured by rever...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(95)00046-r
更新日期:1995-09-01 00:00:00
abstract:OBJECTIVE:Gingival fibroblasts (GFs) can participate in the immune response and play an immune-regulatory role. This study was to evaluate whether GFs can be differently activated and polarized into distinct functional subtypes in response to T helper type 1 (Th1), or Th2 stimuli. DESIGN:Human GFs were stimulated by t...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2019.04.019
更新日期:2019-06-01 00:00:00
abstract:OBJECTIVE:The objective of this study was to determine the influence of masticatory function, dental caries and socioeconomic status on the body mass index (BMI) of preschool children. METHODS:A cross-sectional study was conducted with a sample of 285 children aged three to five years allocated to three groups based o...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2017.04.032
更新日期:2017-09-01 00:00:00
abstract::The periodontal ligament (PDL) contains precursor cells for osteoblasts and cementoblasts. It has been shown that epidermal growth factor (EGF) inhibits dexamethasone-induced differentiation and up-regulates EGF-receptor (EGF-R) expression, whereas EGF-R is down-regulated in the course of differentiation. Thus it was ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(98)00079-x
更新日期:1998-12-01 00:00:00
abstract::Tartrate-resistant acid phosphatase (TRAPase)-positive cells were examined during the early morphogenesis of mouse mandibular first molar teeth, using a simultaneous azo dye-coupling technique and confocal laser scanning microscopy (CLSM). CLSM of paraffin-embedded sections revealed that cells exhibiting TRAPase fluor...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(98)00019-3
更新日期:1998-05-01 00:00:00
abstract::Casein phosphopeptides (CPP) stabilize amorphous calcium phosphate (ACP) and may be used to localize ACP in dental plaque, maintaining a state of supersaturation with respect to tooth enamel, reducing demineralization and enhancing remineralization. The aim here was to investigate these effects by measuring the effect...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(00)00017-0
更新日期:2000-07-01 00:00:00
abstract:UNLABELLED:Growth hormone (GH) stimulates mandibular growth but its effect on the mandibular condylar cartilage is not well understood. OBJECTIVE:This study was designed to understand the influence of GH on mitotic activity and on chondrocytes maturation. The effect of GH on cartilage thickness was also determined. D...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2004.02.004
更新日期:2004-07-01 00:00:00
abstract:OBJECTIVE:To study the ultrastructural alterations induced in Streptococcus mutans (ATCC 25175) incubated with saliva, saliva plus histatin 5 and histatin 5. METHODS:S. mutans incubated with saliva histatin 5 or a combination of both were morphologically analyzed and counted. The results were expressed as (CFU)ml-1. U...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2018.01.004
更新日期:2018-03-01 00:00:00
abstract:OBJECTIVE:To investigate the antimicrobial effect of alpha-bisabolol and tea tree oil alone and in combination against the halitosis-associated Gram-positive bacillus Solobacterium moorei. DESIGN:The inhibitory activity of alpha-bisabolol and tea tree oil against the reference strain S. moorei CCUG39336 and four clini...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2012.08.001
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:To compare proteomics and biological function of human dentin matrix molecules (hDMMs) and bovine dentin matrix molecules (bDMMs). DESIGN:Dentin powder from human or bovine teeth (n = 4) was demineralized in 10% (v/v) ethylenediaminetetraacetic acid for 7 days. The extracts were dialyzed, lyophilized and pro...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2020.104888
更新日期:2020-11-01 00:00:00
abstract:OBJECTIVE:Amelogenesis imperfecta (AI) is a group of clinically and genetically heterogeneous inherited conditions, causing alterations in the structure of enamel and chemical composition of enamel matrix during development. The objective of this study was to compare the clinical, radiographic, histological and immunoh...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2015.06.016
更新日期:2015-09-01 00:00:00
abstract::Five well-characterized foods were used to compare two systems for estimating the cariogenic potential of food: human plaque-acidity using interdental metallic touch electrodes, and cariogenicity testing in rats. Both systems identified the same food as the least cariogenic or acidogenic food and identified the same t...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(85)90089-5
更新日期:1985-01-01 00:00:00
abstract::Neurophysiological exploration of the trigeminal sensory complex was done on 42 cats under ketamine anaesthesia, paying special attention to units receiving a periodontal input. Among 492 cells recorded in the trigeminal sensory complex, 73 responded to mechanical stimulation of the periodontium and were precisely loc...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(83)90138-3
更新日期:1983-01-01 00:00:00
abstract::The aim of this project was to develop an analytical procedure with the required level of sensitivity for the determination of glucose concentrations in small volumes of unstimulated fasting whole saliva. The technique involves high-performance ion-exchange chromatography at high pH and pulsed amperometric detection. ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(95)00121-2
更新日期:1996-02-01 00:00:00
abstract:OBJECTIVE:Streptococcus mutans, known to be a major pathogen of dental caries, is also considered to cause infective endocarditis. Its 120-kDa Cnm protein binds to type I collagen, which may be a potential virulence factor. In this study, we characterized S. mutans clinical strains focusing on the cnm gene encoding Cnm...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2009.11.008
更新日期:2010-01-01 00:00:00
abstract::The presence of human STRO-1(+)/CD146(+) periodontal ligament (PDL) cells has been reported, but obtaining a large amount of these cells is difficult. The purpose of this study was to evaluate the percentages of STRO-1(+)/CD146(+) cells in PDL cells and determine the effects of FGF-2 on the proliferation and multiline...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2011.12.003
更新日期:2012-06-01 00:00:00
abstract:OBJECTIVE:Streptococcus mutans is known to be a primary causative agent of dental caries and its surface proteins have been investigated to specify their association with its virulence. Amongst those, 4 glucan-binding proteins (Gbps) are considered to be important factors due to their glucan-binding properties, of whic...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.09.018
更新日期:2011-03-01 00:00:00
abstract:OBJECTIVE:The anti-cariogenic properties of tea have been suggested for decades. Tea polyphenols, especially epigallocatechin gallate (EGCG), have been shown to inhibit dental plaque accumulation, but the exact mechanisms are not clear at present. We hypothesise that EGCG suppresses gtf genes in Streptococcus mutans at...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2011.10.021
更新日期:2012-06-01 00:00:00
abstract:OBJECTIVE:The aim of the study was to compare the neonatal line width as well as the composition of the pre-natal and post-natal enamel in deciduous teeth of children with cerebral palsy (CP) to deciduous teeth of healthy children. DESIGN:58 extracted or normally exfoliated deciduous teeth were collected for the study...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2019.06.001
更新日期:2019-08-01 00:00:00
abstract::Dehydration by alcohols and concentrated solutions of highly soluble salts caused refractive changes in enamel which were enhanced in the cervical regions. Electron microscopy showed crystals in cervical enamel to vary from normal orientation, size and habit to marked disorientation and reduced size. Consequent increa...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(81)90106-0
更新日期:1981-01-01 00:00:00
abstract::A genomic library of Bacteroides gingivalis W83 chromosomal DNA was constructed in the Escherichia coli lambda (lambda) vector EMBL 4. Three recombinant lambda phages expressing a cloned protease were identified in the library. All three lambda phages contained cloned overlapping DNA fragments from the same region of ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(90)90137-y
更新日期:1990-01-01 00:00:00
abstract:OBJECTIVE:Currently there is still a debate about whether peritubular dentine (PTD) is non-collageneous or collageneous tissue. The chemical composition and structure of human PTD and intertubular dentine (ITD) was re-visited in this study. DESIGN:The dentine tubular region including ITD and PTD prepared from human th...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2011.09.008
更新日期:2012-04-01 00:00:00
abstract::Designing a temporomandibular joint (TMJ) total prosthesis requires the assessment of joint displacements for open/close movements. Current knowledge presents disc-condyle motions as involving both translation and rotation but there may be substantial variations from human to human. The aim of this study is to discrim...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2007.11.010
更新日期:2008-05-01 00:00:00
abstract::Many studies have shown that various myosin isoforms are involved in muscle contraction. A search for specific antibodies directed against the myosin heavy chain (MHC) resulted in the identification of at least two main classes, referred to as MHC type I and type II. In this study, immunohistology and gel electrophore...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(00)00092-3
更新日期:2000-12-01 00:00:00
abstract::The mineralization levels of erupted buccal enamel from 24 deciduous teeth were compared to those of 28 permanent teeth. Sections were prepared in a defined plane using a lapping machine which gave plano-parallel sections. Mineralization levels were recorded by quantitative microradiography at 25 equivalent anatomical...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(89)90130-1
更新日期:1989-01-01 00:00:00
abstract::Smears of buccal mucosa, dorsal surface of the tongue and floor of mouth were taken from 10 patients with histologically confirmed oral lichen planus and 12 healthy age- and sex-matched controls. In buccal smears, no significant differences in cytoplasmic and nuclear areas were observed between lesional, adjacent non-...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(95)00106-9
更新日期:1996-01-01 00:00:00
abstract:OBJECTIVES:The aim of this in vitro study was to evaluate the effectiveness of an iodoform paste and silver-coated abutments in preventing the microbial colonization and leakage through the implant-abutment interface of morse taper and internal hexagon implants. MATERIAL AND METHODS:Seventy-two implants with morse tap...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2019.06.009
更新日期:2019-09-01 00:00:00
abstract:OBJECTIVE:To evaluate the expression of the cytokines IFN-γ, IL-1β, IL-4, IL-6, IL-10, IL-21, IL-22, IL-23, IL-25, IL-31, IL-33, IL-17A, IL-17F, sCD40L, and TNF-α in gingival tissue and intestinal mucosa of patients having both periodontitis and inflammatory bowel disease (IBD) and assess how they cluster in both tissu...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2016.02.018
更新日期:2016-06-01 00:00:00
abstract:BACKGROUND:Integrin-α5 (ITGA5) gene has been reported to be critical for the progression of several cancers. However, the effects of ITGA5 in oral squamous cell carcinoma (OSCC) remain unclear. METHODS:We firstly used bioinformatics methods to analyze the ITGA5 gene expression based on the public dataset. HO1-N-1 and ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2019.03.007
更新日期:2019-05-01 00:00:00