[Gap junctions in the human urinary bladder].

Abstract:

PURPOSE:Gap junctions are intercellular contacts important for the synchronization of muscle cell activity through electrical coupling. Since the role of gap junctions for the function of smooth bladder muscle is still a matter of debate, we investigated the occurrence of gap junctions and the gap junctional protein connexin (Cx) 45 in the detrusor of the nonobstructed stable human bladder. MATERIALS AND METHODS:Detrusor biopsies from 6 patients aged 64 (55-72) years with stable nonobstructed bladders were investigated for the occurrence of gap junctions by electron microscopy, molecular biological techniques and immunohistochemistry. RESULTS:Transmission electron microscopy and freeze fracture showed the presence of gap junction at plasma membranes of detrusor smooth muscle cells. By reverse transcriptase (RT) polymerase chain reaction (PCR) and in situ hybridization, we found an expression of Cx45 in the detrusor. These data were confirmed by immunolocalization of Cx45 on smooth muscle cells. CONCLUSIONS:This study provides morphological as well as molecular biological and immunohistochemical evidence that bladder smooth muscle cells are electrically coupled.

journal_name

Aktuelle Urol

journal_title

Aktuelle Urologie

authors

John H,Wang X,Hauri D,Maake C

doi

10.1055/s-2003-42001

subject

Has Abstract

pub_date

2003-09-01 00:00:00

pages

328-32

issue

5

eissn

0001-7868

issn

1438-8820

journal_volume

34

pub_type

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