Abstract:
:The chromosomal localization of 13 bovine genes was determined using radiation hybrid (RH) mapping. The RH mapping data were in agreement with published data using either linkage, somatic cell hybrids or in situ hybridization. Mutation analysis using single-stranded conformational polymorphism, restriction fragment length polymorphism (RFLP) and sequencing revealed 13 SNPs in four different genes, namely carboxypeptidase E (CPE), uncoupling protein 2 (UCP2), single-minded (Drosophila) homologue 1 (SIM1) and methallothionein IIa (MT2A). With the exception of one mutation in CPE, all other mutations are either silent or are situated in an intron. The polymerase chain reaction RFLP was used on unrelated animals from different cattle breeds for determing allelic distribution.
journal_name
Anim Genetjournal_title
Animal geneticsauthors
Haegeman A,Williams JL,Law A,Van Zeveren A,Peelman LJdoi
10.1046/j.1365-2052.2003.01008.xsubject
Has Abstractpub_date
2003-10-01 00:00:00pages
349-53issue
5eissn
0268-9146issn
1365-2052pii
1008journal_volume
34pub_type
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