Mapping and SNP analysis of bovine candidate genes for meat and carcass quality.

Abstract:

:The chromosomal localization of 13 bovine genes was determined using radiation hybrid (RH) mapping. The RH mapping data were in agreement with published data using either linkage, somatic cell hybrids or in situ hybridization. Mutation analysis using single-stranded conformational polymorphism, restriction fragment length polymorphism (RFLP) and sequencing revealed 13 SNPs in four different genes, namely carboxypeptidase E (CPE), uncoupling protein 2 (UCP2), single-minded (Drosophila) homologue 1 (SIM1) and methallothionein IIa (MT2A). With the exception of one mutation in CPE, all other mutations are either silent or are situated in an intron. The polymerase chain reaction RFLP was used on unrelated animals from different cattle breeds for determing allelic distribution.

journal_name

Anim Genet

journal_title

Animal genetics

authors

Haegeman A,Williams JL,Law A,Van Zeveren A,Peelman LJ

doi

10.1046/j.1365-2052.2003.01008.x

subject

Has Abstract

pub_date

2003-10-01 00:00:00

pages

349-53

issue

5

eissn

0268-9146

issn

1365-2052

pii

1008

journal_volume

34

pub_type

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