Abstract：
:The myb proto oncogene product (c-Myb) is a transcriptional regulator and its expression and function are tightly linked to the cellular entry into S phase and DNA synthesis. It has been shown [Venturelli, D., Travali, S. & Calabretta, B. (1990). Proc. Natl. Acad. Sci. USA, 87, 5963-5967] that inhibition of T-cell proliferation by a myb antisense oligomer is accompanied by down-regulation of DNA polymerase alpha expression. To examine whether the transcription of the DNA polymerase alpha gene is directly regulated by c-Myb, we have identified and characterized the 5' regulatory region of the human DNA polymerase alpha gene. Two major and several minor transcription start sites were identified by nuclease S1 mapping. DNA sequence analysis showed that the promoter region contains no TATA box, one CCAAT box and putative Ap-1, AP-2 and E2F binding sites. In DNAase I footprinting, the bacterially expressed c-Myb protected six sites in the 5' flanking region of the human DNA polymerase alpha gene. However, c-Myb did not activate the DNA polymerase alpha gene promoter in a co-transfection assay. Our results suggest that an unknown factor(s) is required for the c-Myb-induced activation of the DNA polymerase alpha gene promoter, or c-Myb does not directly activate this promoter.
journal_name
Oncogenejournal_title
Oncogeneauthors
Sudo T,Miyazawa H,Hanaoka F,Ishii Ssubject
Has Abstractpub_date
1992-10-01 00:00:00pages
1999-2006issue
10eissn
0950-9232issn
1476-5594journal_volume
7pub_type
杂志文章相关文献
ONCOGENE文献大全abstract：:Pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal human cancers, with 5-year patient survival rates of <5%. Activating mutations in KRAS are the predominant oncogenic drivers of PDAC but are accompanied by additional lower frequency genetic alterations. Our group previously identified the guanine ...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/s41388-019-0985-1
更新日期：2020-01-01 00:00:00
abstract：:Topoisomerase IIalpha (topoIIalpha) is an essential mammalian enzyme that topologically modifies DNA and is required for chromosome segregation during mitosis. Previous research suggests that inhibition of topoII decatenatory activity triggers a G(2) checkpoint response, which delays mitotic entry because of insuffici...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2010.232
更新日期：2010-08-26 00:00:00
abstract：:A large body of physiological evidence shows that either upregulation or downregulation of intracellular c-Myc activity has profound consequences on cell cycle progression. Recent work suggests that c-Myc may stimulate the activity of cyclin E/cyclin-dependent kinase 2 (Cdk2) complexes and antagonize the action of the...
journal_title：Oncogene
pub_type： 杂志文章,评审
doi：10.1038/sj.onc.1202749
更新日期：1999-05-13 00:00:00
abstract：:We have developed a transplantation system that allows us to introduce oncogenes into mouse mammary epithelial cells in culture and then to reconstitute an epithelial tree in vivo from the genetically altered cells. Introduction of the neu oncogene, a transforming homologue of the human proto-oncogene c-erbB-2, produc...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1993-06-01 00:00:00
abstract：:The t(8;21) is associated with 12-15% of acute myelogenous leukemias of the M2 subtype. The translocation results in the fusion of two genes, AML1 (CBFA2) on chromosome 21 and ETO (MTG8) on chromosome 8. AML1 encodes a DNA binding factor; the ETO protein product is less well characterized, but is thought to be a trans...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1202412
更新日期：1999-02-11 00:00:00
abstract：:A previous report demonstrated that AP-2alpha favors the survival of ovarian cancer patients by clinical findings. However, the functional roles of AP-2alpha in human ovarian cancers have not been determined. To clarify the roles, we overexpressed AP-2alpha in SKOV3 human ovarian cancer cells, which originally possess...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1207723
更新日期：2004-07-15 00:00:00
abstract：:Epithelial-mesenchymal transition (EMT) facilitates cancer invasion and metastasis and thus accelerates cancer progression. p21-activated kinase 4 (PAK4) is a critical regulator of prostate cancer (PC) progression. Here, we report that PAK4 activation promotes PC progression through the EMT regulator Slug. We find tha...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/s41388-018-0327-8
更新日期：2018-09-01 00:00:00
abstract：:To gain better understanding of the molecular alterations responsible for the aggressive growth potential of epidermal growth factor receptor (EGFR)-positive breast cancers, we utilized an expression cloning strategy to seek gene products that mediate the EGF-independent growth of human breast cancer cells. A retrovir...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1204537
更新日期：2001-07-05 00:00:00
abstract：:While advances in laboratory automation has dramatically increased throughout of compound screening efforts, development of robust cell-based assays in relevant disease models remain resource-intensive and time-consuming, presenting a bottleneck to drug discovery campaigns. To address this issue, we present a modified...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/s41388-018-0274-4
更新日期：2018-08-01 00:00:00
abstract：:Replication origins are 'licensed' for a single initiation event by loading Mcm2-7 complexes during late mitosis and G1. Licensing is blocked at other cell cycle stages by the activity of cyclin-dependent kinases and a small protein called geminin. Here, we describe the effects of over-expressing a non-degradable form...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1205910
更新日期：2002-09-26 00:00:00
abstract：:Beta-transducin repeats-containing proteins (beta-TrCP) serve as the substrate recognition subunits for the SCFbeta-TrCP E3 ubiquitin ligases. These ligases ubiquitinate specifically phosphorylated substrates and play a pivotal role in the regulation of cell division and various signal transduction pathways, which, in...
journal_title：Oncogene
pub_type： 杂志文章,评审
doi：10.1038/sj.onc.1207389
更新日期：2004-03-15 00:00:00
abstract：:Cell migration is an integral component of metastatic disease. The ability of cells to transit between mesenchymal and amoeboid modes of migration has complicated the development of successful therapies designed to target cell migration as a means of inhibiting metastasis. Therefore, investigations of the mechanisms t...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2010.516
更新日期：2011-03-10 00:00:00
abstract：:To identify tyrosine kinases that may regulate regeneration of the mammalian intestinal epithelium, we amplified portions of the catalytic domains of protein kinases expressed in intestinal crypt cells, using the polymerase chain reaction technique with primers directed against two invariant amino acid sequence motifs...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1994-07-01 00:00:00
abstract：:Glial cell line-derived neurotrophic factor (GDNF) family ligands (GFLs) all signal through the transmembrane receptor tyrosine kinase RET. The signalling complex consists of GFLs, GPI-anchored ligand binding GDNF family receptor alphas (GFRalphas) and RET. Signalling via RET is required for the development of the ner...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1210161
更新日期：2007-05-31 00:00:00
abstract：:Wild-type human p53 protein is able to self-associate and consists predominantly of homotetramers in solution. In earlier work we identified the protein sequence motifs involved in p53 quaternary structure and showed that while monomeric p53 protein retains tumour suppressor function, monomeric tumour mutant p53 lacks...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
abstract：:We report the detailed molecular characterization of a human parathyroid adenoma with a clonal parathyroid hormone gene rearrangement. This rearrangement is similar to one we characterized recently in an independent adenoma. In these two, plus a third partially characterized adenoma, one allele of the PTH gene, on 11p...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1991-03-01 00:00:00
abstract：:Bladder cancer is one of the most common causes of death in industrialized countries. New tumor markers and therapeutic approaches are still needed to improve the management of bladder cancer patients. Choline kinase-alpha (ChoKalpha) is a metabolic enzyme that has a role in cell proliferation and transformation. Inhi...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2009.91
更新日期：2009-07-02 00:00:00
abstract：:A novel oncogene, rsc (rabbit squamous cell carcinoma), has been identified from a DMBA-induced rabbit squamous cell carcinoma using gene transfer and the nude mouse tumorigenesis assay. A full-length cDNA has been isolated and sequenced. rsc has potent tumorigenic activity in nude mice (latency <4 weeks), but does no...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1200950
更新日期：1997-03-20 00:00:00
abstract：:Protein kinases are known to undergo phosphorylation to regulate their activity. To determine whether the protein kinase activity of p37v-mos was similarly regulated, we investigated the influence of two well known protein kinases, namely protein kinase C and protein kinase A, on the activity of p37v-mos in vivo. NIH3...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
abstract：:A hallmark of plasma cells is the expression of syndecan-1, which has major functions in epithelial cells, in particular as the coreceptor of heparin-binding growth factors. We previously found that heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a growth factor for malignant plasma cells. As am...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1208536
更新日期：2005-05-12 00:00:00
abstract：:Stimulator of interferon genes (STING) is a cellular sensor that controls cytosolic DNA-activated innate immune signaling. We have previously demonstrated that STING-deficient mice are resistant to carcinogen-induced skin cancer, similar to myeloid differentiation primary response gene 88 (MyD88) deficient mice, since...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2014.457
更新日期：2015-10-08 00:00:00
abstract：:Jun : Fos and Jun : ATF complexes represent two classes of AP-1 dimers that (1) preferentially bind to either heptameric or octameric AP-1 binding sites, and (2) are differently regulated by cellular signaling pathways and oncogene products. To discriminate between the functions of Jun : Fos, Jun : ATF and Jun : Jun, ...
journal_title：Oncogene
pub_type： 杂志文章,评审
doi：10.1038/sj.onc.1204239
更新日期：2001-04-30 00:00:00
abstract：:The B-raf/c-Rmil proto-oncogene belongs to the raf/mil family of serine/threonine protein kinases. It encodes multiple protein isoforms resulting from alternative splicing of two exons located upstream of the kinase domain. Recent studies suggested that B-Raf could be the intermediate molecule between Ras and Mek-1 (M...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1995-04-20 00:00:00
abstract：:The reversible interaction of the retinoblastoma susceptibility gene product (Rb) with the cellular transcription factor E2F has recently been demonstrated. Activation of the adenovirus E2a promoter by the products of the viral E1a gene correlates with the ability of both early E1a proteins to sequester Rb, thereby re...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
abstract：:Survivin is a member of the inhibitor of apoptosis protein family and has an essential role in mitosis. Survivin is overexpressed in a large variety of human cancers and represents an attractive target for cancer therapy. Epidermal growth factor receptor and Her/neu-transformed human tumors in particular exhibit high ...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2011.377
更新日期：2012-04-12 00:00:00
abstract：:We have evaluated the transcriptional activation of a human p21 promoter reporter construct by transfection of BRCA1 expression constructs into tumorigenic and nontumorigenic human breast cell lines. Two cell lines with wildtype p53 (MCF-7 and MCF10A) demonstrated transcriptional activation of the p21 promoter by full...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1204025
更新日期：2000-12-14 00:00:00
abstract：:E2a/Pbx1 is a fusion oncoprotein resulting from the t(1;19) translocation found in human pre-B acute lymphocytic leukemia and in a small number of acute T-lymphoid and myeloid leukemias. It was previously suggested that E2a/Pbx1 could cooperate with normal or oncogenic signaling pathways to immortalize myeloid and lym...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1209777
更新日期：2007-01-11 00:00:00
abstract：:Chromosome 8 loss of heterozygosity (LOH) in cancer of the urinary bladder is associated with high tumour grade and stage. We have screened 193 cases of transitional cell carcinoma (TCC) of the bladder using 30 microsatellite polymorphisms on chromosome 8. Forty three cases (22%) showed LOH on 8p, the majority of whic...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1996-03-07 00:00:00
abstract：:We recently reported the cloning and sequencing of the mouse A-myb proto-oncogene cDNA and the abundant expression of this mRNA primarily in the testis of adult mice. The A-myb mRNA is detectable by in situ hybridization specifically in the spermatogenic cells, and is downregulated during terminal differentiation. A l...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1996-09-19 00:00:00
abstract：:Human papillomaviruses are the causative agents of cervical cancer. Previous studies have shown that loss of the viral E2 protein during malignant progression is an important feature of HPV-induced malignancy due to the resulting uncontrolled expression of the viral oncoproteins E6 and E7. We now show however that the...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1208701
更新日期：2005-08-04 00:00:00
