Abstract:
:Cells of the macrophage lineage are a major source of various cytokines and hematopoietic growth factors. With regard to the growth factors acting on cells of their own lineage, macrophage colony-stimulating factor (M-CSF) has been proven to be secreted by monocytes (MO) and macrophages (MAC), whereas the production of granulocyte-macrophage colony-stimulating factor (GM-CSF) by human MO/MAC is under debate. Here we report that in elutriation-purified MO, as well as in MAC derived from cultured MO, GM-CSF m-RNA was regularly induced by LPS. In MO the GM-CSF message was still detectable 18 h after stimulation under serum-free conditions, but in contrast was already lost at this time point in MAC. Secreted GM-CSF protein was detected in the culture medium using a sandwich ELISA. Furthermore, a factor-dependent cell line (M-07) was used for a biological assay. Here, a neutralizing anti GM-CSF antibody specifically blocked the proliferation-inducing activity of MO/MAC supernatants. Whereas only small amounts of GM-CSF were detected in MO, its secretion increased severalfold upon MO-to-MAC differentiation in vitro. A similar increase upon in vitro maturation of MO was observed for the production of granulocyte colony-stimulating factor. The highest amounts of GM-CSF (up to 2.8 ng/10(6) cells) were produced by MAC that had been derived from MO cultured under serum-free conditions in the presence of 0.5 mg/ml albumin as the only medium supplement.
journal_name
Ann Hematoljournal_title
Annals of hematologyauthors
Krause SW,Kreutz M,Zenke G,Andreesen Rdoi
10.1007/BF01696222subject
Has Abstractpub_date
1992-04-01 00:00:00pages
190-5issue
4eissn
0939-5555issn
1432-0584journal_volume
64pub_type
杂志文章abstract::High-dose chemotherapy with autologous peripheral blood stem cell transplantation (PBSCT) includes the risk of infectious complications due to neutropenia and therapy-induced immune deviation. In order to understand early immune recovery in this situation, we analyzed the distribution of cell subsets by flow cytometry...
journal_title:Annals of hematology
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journal_title:Annals of hematology
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journal_title:Annals of hematology
pub_type: 杂志文章,多中心研究
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journal_title:Annals of hematology
pub_type: 杂志文章
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更新日期:1992-05-01 00:00:00
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journal_title:Annals of hematology
pub_type: 杂志文章,评审
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更新日期:2004-02-01 00:00:00
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journal_title:Annals of hematology
pub_type: 杂志文章,评审
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journal_title:Annals of hematology
pub_type: 临床试验,杂志文章
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journal_title:Annals of hematology
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journal_title:Annals of hematology
pub_type: 临床试验,杂志文章
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journal_title:Annals of hematology
pub_type: 临床试验,杂志文章
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journal_title:Annals of hematology
pub_type: 杂志文章,多中心研究
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更新日期:2021-01-01 00:00:00
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journal_title:Annals of hematology
pub_type: 临床试验,杂志文章
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journal_title:Annals of hematology
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更新日期:2003-12-01 00:00:00
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journal_title:Annals of hematology
pub_type: 杂志文章
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更新日期:2010-05-01 00:00:00
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journal_title:Annals of hematology
pub_type: 杂志文章,多中心研究
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更新日期:2016-12-01 00:00:00
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