Abstract:
:In vitro studies suggest that haploinsufficiency is involved in the pathogenesis of myelodysplastic syndromes (MDS). In patients with del5q cytogenetic abnormality, RPS-14 and microRNAs (miRNAs) play a major role. In a multicenter phase II single-arm trial with lenalidomide in anemic primary del5q MDS patients with low- or int-1 risk IPSS, biological changes from baseline were investigated. Gene expression profiling of selected genes was performed (TaqMan® Low Density Array Fluidic card, Applied Biosystems PRISM® 7900HT) and normalized against the expression of the 18S housekeeping gene from a pool of healthy subjects. Thirty-two patients were evaluated at baseline and after 3 and 6 months of treatment. RPS-14, miR-145, and miR-146 were downregulated at baseline and significantly increased during treatment. Nuclear factor kappa B, IL-6, interferon regulatory factor-1, IFNγ-R2, IL-2, and many genes in the apoptotic pathways (TNF, IL-1B, and IL-10) were upregulated at baseline and significantly downregulated during lenalidomide treatment, while forkhead box P3, FAS, IFNγ, IL-12A, and IL-12B were downregulated at baseline and progressively upregulated during treatment. The crucial role of aberrant immunological pathways and haploinsufficiency in the pathogenesis of del5q MDS is confirmed in the present patient setting. Our results indicate that lenalidomide may act through defined immunological pathways in this condition.
journal_name
Ann Hematoljournal_title
Annals of hematologyauthors
Oliva EN,Cuzzola M,Aloe Spiriti MA,Poloni A,Laganà C,Rigolino C,Morabito F,Galimberti S,Ghio R,Cortelezzi A,Palumbo GA,Sanpaolo G,Finelli C,Ricco A,Volpe A,Rodà F,Breccia M,Alimena G,Nobile F,Latagliata Rdoi
10.1007/s00277-012-1569-0subject
Has Abstractpub_date
2013-01-01 00:00:00pages
25-32issue
1eissn
0939-5555issn
1432-0584journal_volume
92pub_type
杂志文章abstract::Activation-induced cytidine deaminase (AID) is a mutator enzyme essential for somatic hypermutation (SHM) and class switch recombination (CSR) during effective adaptive immune responses. Its aberrant expression and activity have been detected in lymphomas, leukemias, and solid tumors. In chronic lymphocytic leukemia (...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-018-3520-5
更新日期:2019-02-01 00:00:00
abstract::We tested two Fc gamma receptor I (Fc gamma RI); six Fc gamma RII; and six Fc gamma RIII-specific monoclonal antibodies (mAb) for their capacity to inhibit monocyte and polymorphonuclear granulocyte (PMN) immune phagocytosis which is mediated by Fc gamma R. We used human red blood cells (rbc) coated with hIgG1 or mIgG...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s002770050249
更新日期:1997-01-01 00:00:00
abstract::In multiple myeloma, the polymerase chain reaction (PCR) of the Ig heavy chain with allele-specific oligonucleotide (ASO) primers is a common and well-described method of identifying the tumor clone in peripheral blood (PB), bone marrow (BM) or leukapheresis products (LA). A factor which is crucial to the detection of...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s002770000245
更新日期:2001-02-01 00:00:00
abstract::A combination of chromosomal translocations associated with bcl-2 re-arrangement (t(14;18)) and c-myc re-arrangement (t(8;14), t(8;22), or t(2;8)) is a rare event. We describe the first cell line exhibiting t(14;18) and t(8;22), which will enable us to study the interactions of bcl-2 and c-myc systematically. Cell cul...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-007-0313-7
更新日期:2007-11-01 00:00:00
abstract::Gastric MALT lymphoma is closely associated with Helicobacter pylori infection. Bacterial eradication therapy comprising clarithromycin is the first-line treatment in gastric MALT lymphoma patients. However, antimicrobial resistance to clarithromycin has been increasing in Europe, and thus far, it has not been examine...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-016-2672-4
更新日期:2016-06-01 00:00:00
abstract::Asplenic and hyposplenic patients have an increased risk for overwhelming pneumococcal infections, even several decades after splenectomy. Pneumococcal vaccination and daily oral administration of penicillin V are recommended to prevent such infections, 2-5 years after splenectomy, and for at least 5 years in children...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-003-0779-x
更新日期:2004-01-01 00:00:00
abstract::The present study attempted to build a single nucleotide polymorphism (SNP)-based risk model for predicting overall survival (OS) and event-free survival (EFS) in patients with core binding factor acute myeloid leukemia (CBF-AML). Adopting genome-wide SNP array using Affymetrix SNP array 6.0, we analyzed 868,157 SNPs ...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-018-3260-6
更新日期:2018-06-01 00:00:00
abstract::A 77-year-old man developed pneumonitis while on chlorambucil therapy for chronic lymphocytic leukemia, with a cumulative dose of 2700 mg. The condition improved promptly with the discontinuation of the drug and initiation of steroids. A case report and review of the literature are presented in this paper. ...
journal_title:Annals of hematology
pub_type: 杂志文章,评审
doi:10.1007/s002770050418
更新日期:1998-07-01 00:00:00
abstract::Aberrant promoter methylation of RASSF6 and RASSF10 occurs at a high frequency in acute lymphoblastic leukemia (ALL). Because of the complexity of the current minimal residual disease (MRD) detecting-methods, the DNA methylation status of the RASSF6 and RASSF10 genes could potentially become biomarkers for the assessm...
journal_title:Annals of hematology
pub_type: 临床试验,杂志文章
doi:10.1007/s00277-019-03775-y
更新日期:2019-12-01 00:00:00
abstract::This study aimed to determine whether dose-dense therapy improves 3-year survival over the standard therapy for untreated aggressive lymphoma. One hundred and fifteen patients with untreated aggressive lymphoma were stratified by center, age, and international prognostic index and randomized to one of two treatment ar...
journal_title:Annals of hematology
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00277-009-0811-x
更新日期:2010-03-01 00:00:00
abstract::The general impact of blood donation on iron status was studied in a population survey comprising 1359 nonpregnant Danish women in age cohorts of 30, 40, 50, and 60 years; 809 were premenopausal and 550 postmenopausal; 180 (13%) were blood donors. Iron stores were assessed by serum (S)-ferritin and hemoglobin (Hb). Hb...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/BF01714957
更新日期:1991-07-01 00:00:00
abstract::Approximately, 4-11 % of the patients with idiopathic venous thrombosis (VT) show protein C (PC) deficiency. The molecular pathology of PC deficiency was analyzed in 102 patients; 98 healthy controls were also studied to assess the association of various polymorphisms with reduced PC levels. PROC gene mutations were d...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-012-1483-5
更新日期:2012-09-01 00:00:00
abstract::The clinical impact of KIT mutations in core binding factor acute myeloid leukemia (CBF-AML) is still unclear. In the present study, we analyzed the prognostic significance of each KIT mutation (D816, N822K, and other mutations) in Japanese patients with CBF-AML. We retrospectively analyzed 136 cases of CBF-AML that h...
journal_title:Annals of hematology
pub_type: 临床试验,杂志文章
doi:10.1007/s00277-017-3074-y
更新日期:2017-10-01 00:00:00
abstract::Post-transplantation cyclophosphamide (PTCy) demonstrated effectiveness to prevent GVHD after haploidentical hematopoietic cell transplantation (HCT). Reducing toxicities with a maximized efficacy is still challenging in HCT. In this retrospective study, we analyzed the safety and efficacy of transplantation from a 1-...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-019-03673-3
更新日期:2019-06-01 00:00:00
abstract::The impact of intraocular involvement (IOL) in primary CNS lymphoma (PCNSL) has not been sufficiently evaluated. Here, we present the analysis of IOL in the only completed randomized phase III trial in PCNSL. The G-PCNSL-SG1 study evaluated the role of whole-brain radiotherapy in primary therapy of PCNSL. Data of the ...
journal_title:Annals of hematology
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1007/s00277-014-2212-z
更新日期:2015-03-01 00:00:00
abstract::We report about a 28-year-old woman with relapsed mantle cell lymphoma (MCL, centrocytic lymphoma according to the Kiel classification) refractory to salvage chemotherapy. The patient underwent allogeneic bone marrow transplantation from a HLA-identical brother after myeloablative chemotherapy consisting of busulfan, ...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s002770000196
更新日期:2000-10-01 00:00:00
abstract::An improvement in quality of life and survival occurred among thalassemia major (TM) patients: pregnancy in such patients has become a reality. Safe pregnancy and delivery require efforts to ensure the best outcomes. Between 2007 and 2016, 30 TM patients had 37 pregnancies. We analyzed the hematological parameters bef...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-017-2979-9
更新日期:2017-06-01 00:00:00
abstract::We have studied the effects of recombinant human thrombopoietin (TPO, mpl ligand) on the megakaryocyte colony formation from control human bone marrow cells, human leukemia cells at diagnosis, and human bone marrow cells after induction chemotherapy for acute leukemias. In the control human bone marrow cells from four...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s002770050408
更新日期:1998-07-01 00:00:00
abstract::Nondeletional hereditary persistence of fetal hemoglobin (nd-HPFH), a rare hereditary condition resulting in elevated levels of fetal hemoglobin (Hb F) in adults, is associated with promoter mutations in the human fetal globin (HBG1 and HBG2) genes. In this paper, we report a novel type of nd-HPFH due to a HBG2 gene p...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-008-0643-0
更新日期:2009-06-01 00:00:00
abstract::We screened for increased osmotic fragility of erythrocytes in 1464 healthy German blood donors. The osmotic fragility was determined by an acidified glycerol lysis test (AGLT) using glycerol-sodium phosphate-buffered NaCl solution. Since the original test described by Zanella et al. [23] showed only low specificity f...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/BF01715351
更新日期:1992-02-01 00:00:00
abstract::A 72-year-old-man with night sweats and a low-grade fever was found to have bilateral adrenal enlargement associated with incipient adrenal insufficiency. Without any intervention, these adrenal lesions regressed spontaneously, accompanied by disappearance of clinical symptoms. Seven months later, however, the lesions...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s002770100335
更新日期:2001-09-01 00:00:00
abstract::The objective of this study is to compare and evaluate the diagnostic value of hereditary spherocytosis (HS) by three screening tests, comparing mean spherical corpuscular volume (MSCV) to mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and flow cytometric osmotic fragility test. Perip...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-014-2270-2
更新日期:2015-05-01 00:00:00
abstract::Spontaneous splenic rupture is a rare, though life-threatening complication of some hematological malignancies and is even more infrequent as the initial symptom of acute lymphoblastic leukemia. We describe the fourth case, to our knowledge, of acute lymphoblastic leukemia presenting as splenic rupture, and for the fi...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/BF01727419
更新日期:1994-03-01 00:00:00
abstract::To investigate the problem of allogeneic bone marrow transplantation (allo-BMT) for advanced stage patients, we retrospectively analyzed 24 consecutive patients who underwent allo-BMT in the non-remission stage. Twenty-four patients (19 males and 5 females) with acute leukemia, chronic myelogenous leukemia, and malign...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-002-0506-z
更新日期:2002-10-01 00:00:00
abstract::We found increased numbers of CD28-negative T cells in a patient with multicentric Castleman's disease (MCD), who also had significantly decreased interleukin-2 (IL-2) production and impaired T-cell proliferation. The presence of CD28-negative T cells may be indicative of a functional T-cell defect in MCD. ...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s002770050228
更新日期:1996-10-01 00:00:00
abstract::MicroRNAs are short noncoding RNAs, known regulators of several signaling pathways cell differentiation and proliferation, development, and apoptosis, which are deregulated in acute leukemia. Mixed lineage leukemia (MLL) gene encodes a protein with histone methyltransferase activity, which is essential for the fine tu...
journal_title:Annals of hematology
pub_type: 杂志文章,评审
doi:10.1007/s00277-013-1803-4
更新日期:2013-11-01 00:00:00
abstract::Five major subtypes of human T-lymphotropic virus type I (HTLV-I) have been proposed: cosmopolitan, Japanese, West African, Central African, and Melanesian. Based on nucleotide variations specific to particular subtypes, it was possible to genotype HTLV-I rapidly by restriction fragment length polymorphism (RFLP) stud...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s002770050213
更新日期:1996-09-01 00:00:00
abstract::Expression of long non-coding RNA KIAA0125 has been incorporated in various gene expression signatures for prognostic prediction in acute myeloid leukemia (AML) patients, yet its functions and clinical significance remain unclear. This study aimed to investigate the clinical and biological characteristics of AML beari...
journal_title:Annals of hematology
pub_type: 临床试验,杂志文章
doi:10.1007/s00277-020-04358-y
更新日期:2021-02-01 00:00:00
abstract::During the last few years, new insights into the biology of mantle cell lymphoma have been obtained. However, with a median survival of only 3 years, mantle cell lymphoma remains the lymphoma subtype with the poorest prognosis. At initial diagnosis most patients present with advanced Ann Arbor stage III or IV and conv...
journal_title:Annals of hematology
pub_type: 杂志文章,评审
doi:10.1007/s00277-003-0774-2
更新日期:2004-02-01 00:00:00
abstract::Polycythemias or erythrocytoses in childhood and adolescence are very rare. Systematic data on the clinical presentation and laboratory evaluations as well as on treatment regimens are sparse. The diagnostic program in absolute erythrocytosis includes extensive clinical, hematological, biochemical, and molecular biolo...
journal_title:Annals of hematology
pub_type: 杂志文章,评审
doi:10.1007/s00277-004-0985-1
更新日期:2005-03-01 00:00:00