Effects of Ni(2+), Co(2+), and Mn(2+) on desensitized butyrylcholinesterase prepared from human serum.

Abstract:

:Human serum butyrylcholinesterase (BChE) has been converted into a stable but less active desensitized form when heated at 45 degrees C for 24 h. The desensitized BChE follows Michaelis-Menten kinetics, whereas native enzyme exhibits slightly negative cooperativity with respect to butyrylthiocholine binding. In this study, we investigated the effects of Ni2+, Co2+, and Mn2+ on the desensitized BChE. It is found that all three ions were noncompetitive inhibitors of the desensitized BChE, and Ki values have been determined as 7.816 +/- 1.060 mM, 48.722 +/- 4.635 mM, and 84.795 +/- 5.249 mM for Ni2+, Co2+, and Mn2+, respectively. In our previous study, these ions were linear mixed-type inhibitors of the native BChE. This finding confirms that desensitized BChE changes to a different conformation than native BChE. From the comparison of Ki values of the trace elements, it can be said that Ni2+ is a more effective inhibitor of the desensitized BChE than Co2+ and Mn2+.

journal_name

Biol Trace Elem Res

authors

Cengiz D,Cokugras AN,Tezcan EF

doi

10.1385/BTER:93:1-3:55

subject

Has Abstract

pub_date

2003-07-01 00:00:00

pages

55-62

issue

1-3

eissn

0163-4984

issn

1559-0720

pii

BTER-93-1-3-55

journal_volume

93

pub_type

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