Abstract:
:The regulation of osteoblast proliferation is a key factor in maintaining bone mass. The enhancement of this process can be achieved by stimulating the proliferation of these cells. Mechanical stimulation is one of the important enhancing factors, but the exact cellular mechanisms of mechanical stimulation, i.e., mechanotransduction, are unknown. In order to investigate the role of the cytoskeleton components in mechanotransduction for cell proliferation, I compared the total DNA content in cultured replicates of osteoblast-like cells derived from three human donors following their exposure to enhancing mechanical stimulation, with and without added specific microtubular and microfilament polymerization blockers (Colchicin and Cytochalasin D, respectively). The results revealed the essential and unique role of the microtubular component of the cytoskeleton in mechanotransduction for proliferation by showing that Colchicin blocked the expected increase in the DNA content after mechanical stimulation of the cultured replicates without altering the total DNA content in replicates at static conditions. Conversely, a specific blockage of the microfilament polymerization presented uniform cytotoxic effect in both static and biomechanically active environments. Since previous reports indicated the essential role of microfilament polymerization for the osteoblast metabolic activity, the results of this study further support the hypothesis that the mechanotransduction mechanisms for proliferation and metabolic activity are mediated by different intracellular pathways.
journal_name
Hum Exp Toxicoljournal_title
Human & experimental toxicologyauthors
Rosenberg Ndoi
10.1191/0960327103ht362oasubject
Has Abstractpub_date
2003-05-01 00:00:00pages
271-4issue
5eissn
0960-3271issn
1477-0903journal_volume
22pub_type
杂志文章abstract::Hydrogen sulfide (H2S) is an endogenously produced gaseous messenger that participates in regulation of cardiovascular functions. This study evaluates the possible protective effect of H2S in cardiovascular dysfunction induced by cecal ligation and puncture (CLP) in rats. After 24 h of induction of CLP, heart rate (HR...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327114564794
更新日期:2015-10-01 00:00:00
abstract::The mechanism of doxorubicin (DOX)-induced cardiotoxicity remains controversial. Wistar rats (n = 66) received DOX injections intraperitoneally and were randomly assigned to 2 experimental protocols: (1) rats were killed before (-24 h, n = 8) and 24 h after (+24 h, n = 8) a single dose of DOX (4 mg/kg body weight) to ...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327113512342
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abstract::In responding to Drs Calabrese and Baldwin's question, 'At what point, if ever, should hormesis be employed as the principal dose response default assumption in risk assessment?', we examined the benefits of replacing traditional dose-response with hormesis. In general, hormesis provides more complete useful informati...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1191/0960327105ht521oa
更新日期:2005-05-01 00:00:00
abstract::1. Neonatal treatment of rats with vitamin D3 resulted in a change of sexual behavior in adulthood. 2. 2.5 mg vitamin D3 completely inhibited the ejaculation of males without any apparent influence on sexual desire. 250 mg vitamin D3 influenced both the desire and ejaculation. 3. Sexual activity of females was depress...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719601500704
更新日期:1996-07-01 00:00:00
abstract::Comparisons on a linear and the Rozman logarithmic scale for dosage versus carcinogenicity in rodents are presented for methyl eugenol (ME), nitrosodiethylamine (NDEA), ethyl carbamate (EC) and 2-acetylaminofluorene (AAF). Each of these chemicals has been shown to be carcinogenic in experimental animals and, in additi...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1191/0960327105ht525oa
更新日期:2005-06-01 00:00:00
abstract:BACKGROUND:The widespread use of synthetic cannabinoids (SCs) among youth has become an important public health problem. Several life-threatening side effects of SC have been reported, including cardiovascular, gastrointestinal, neurological, renal, metabolic, ophthalmologic, and pulmonary effects, besides skin toxicit...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327120930057
更新日期:2020-11-01 00:00:00
abstract::Celecoxib has been shown to have antitumor effect in previous studies but the mechanisms are unclear. The effect of celecoxib on cytosolic Ca(2+) concentrations ([Ca(2+)]i) and viability in HA59T human hepatoma cells was explored. The Ca(2+)-sensitive fluorescent dye fura-2 was applied to measure [Ca(2+)]i. Celecoxib ...
journal_title:Human & experimental toxicology
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doi:10.1177/0960327112472996
更新日期:2014-11-01 00:00:00
abstract::Substance misuse among street children is a significant problem in developing countries. Volatile substances are the most abused agents. According to case reports, chronic renal diseases are common among substance-abusing street children. In this study, we examined the renal findings of 42 volatile substance-abusing s...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327108092292
更新日期:2008-06-01 00:00:00
abstract::Arsenic is a naturally occurring environmental toxicant, chronic exposure to arsenic can cause multiorgan damage, except for typical skin lesions, liver damage is the main problem for health concern in population with arsenic poisoning. Abnormal apoptosis is closely related to liver-related diseases, and p53 is one of...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327120988880
更新日期:2021-01-27 00:00:00
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journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1191/0960327105ht539oa
更新日期:2005-07-01 00:00:00
abstract:AIM:Diazinon (DZN) is one of the most important organophosphorus compounds used to control pests in agriculture in many countries. Several studies have shown that exposure to DZN may alter protein expression in the liver. In order to further investigate the mechanism of DZN toxicity, differentially expressed ATP-intera...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327115619771
更新日期:2016-10-01 00:00:00
abstract::Alcohol levels were measured in 15 cerebrospinal fluid (CSF) samples and 14 blood samples from grade III and IV male alcoholic patients with signs of nervous system involvement, and compared with levels detected in 11 CSF samples and 11 blood samples from abstemious patients or patients with grade I or II alcoholism w...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719201100313
更新日期:1992-05-01 00:00:00
abstract::The majority of households in rural India still rely on unprocessed solid biomass for domestic energy. The aim of this study was to investigate whether chronic exposure to biomass smoke causes activation of leukocytes and the formation of leukocyte-platelet aggregates. We conducted flow cytometric analysis of beta2 Ma...
journal_title:Human & experimental toxicology
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doi:10.1177/0960327106074603
更新日期:2006-11-01 00:00:00
abstract::Recent years have seen substantial shifts in cultural attitudes towards cannabis for medical and recreational use. However, legalizing recreational marijuana may have adverse effects on individual and public health. As the most widely used illicit agent, cannabis is commonly reported to disrupt learning and memory. Un...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327119886037
更新日期:2020-03-01 00:00:00
abstract::Sepsis, often initiated by an infection, is a state of disrupted inflammatory homeostasis. There is increasing evidence that oxidative stress has an important role in the development of sepsis-induced multiorgan failure. Resveratrol (RV) is a polyphenolic compound found in the skin of red fruits, such as mulberries an...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327112467047
更新日期:2013-10-01 00:00:00
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journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327115605440
更新日期:2015-12-01 00:00:00
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journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719101000104
更新日期:1991-01-01 00:00:00
abstract::Paraquat (PQ) is a highly toxic herbicide that is used in most of the countries without restriction. The cytotoxic effect of PQ is mediated by radicals, which are the products of PQ reduction in cells. The anti-oxidative action of captopril, an angiotensin-converting enzyme inhibitor, appears to be through its ability...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327107084533
更新日期:2007-10-01 00:00:00
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journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719401300405
更新日期:1994-04-01 00:00:00
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journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327111407230
更新日期:2011-12-01 00:00:00
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journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327113499168
更新日期:2014-05-01 00:00:00
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journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327109105405
更新日期:2009-05-01 00:00:00
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journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1191/0960327103ht384oa
更新日期:2003-08-01 00:00:00
abstract::Dimethylacetamide (DMAc) is a skin-penetrating solvent able to induce hepatic damage after chronic exposure. Previous research has indicated that metabolism may be saturated at its present TLV/TWA (10 ppm). Biological monitoring of monomethylacetamide (MMAc), the primary metabolite of DMAc, might therefore underestima...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/096032719301200206
更新日期:1993-03-01 00:00:00
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journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1191/0960327102ht291oa
更新日期:2002-09-01 00:00:00
abstract::A key aim of toxicology is the prevention of adverse effects due to toxic hazards. Therefore, the dissemination of toxicology research findings must confront two important challenges: one being the lack of information on the vast majority of potentially toxic industrial chemicals and the other being the strict criteri...
journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327115601762
更新日期:2015-12-01 00:00:00
abstract::This study investigated the main target sites of chlorpyrifos (CPF), its effect on biochemical indices, and the pathological changes observed in rat liver and kidney function using gas chromatography/mass spectrometry. Adult female Wistar rats (n = 12) were randomly assigned into two groups (one control and one test g...
journal_title:Human & experimental toxicology
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doi:10.1177/0960327115614384
更新日期:2016-09-01 00:00:00
abstract::This study was designed to determine the levels of prolactin, leptin, osteopontin, and follistatin in workers chronically and subacutely exposed to lead compounds. The examined population consisted of three groups. The first group was composed of 56 male workers who were chronically exposed to lead for 13.38 ± 10.38 y...
journal_title:Human & experimental toxicology
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更新日期:2017-06-01 00:00:00
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journal_title:Human & experimental toxicology
pub_type: 杂志文章
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更新日期:2016-09-01 00:00:00
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journal_title:Human & experimental toxicology
pub_type: 杂志文章
doi:10.1177/0960327112446818
更新日期:2012-12-01 00:00:00