Abstract:
:Loss of epithelial cell polarity, which can arise following disruption of tight junctions (TJs), is a precursor to the care-fully orchestrated removal of moribund cells from epithelia in apoptosis. Ordinarily, this cycle of events has minimally disruptive effects on the function of the epithelial barrier, but some agents have been identified that induce apoptosis and promote epithelial leakiness. The allergen Der p 1 is a cysteine peptidase that cleaves TJ adhesion proteins and induces apoptosis in epithelial cells. This suggests the possibility that, at least for some inducers of apoptosis, these events might be causally linked. We report here that Der p 1 induces epithelial apoptosis before outright cell detachment and that apoptosis occurs within the same time span as increased paracellular permeability in polarized epithelial monolayers. Whilst TJ-deficient BEAS-2B cells were resistant to Der p 1-induced apoptosis, the cell line 1HAEo-, which was also TJ deficient, was sensitive to Der p 1, providing evidence against TJ proteolysis as a cause of apoptosis. To provide direct evidence, we propagated cells that normally express TJs in low calcium medium that prevented intercellular junction assembly. These cells retained full susceptibility to Der p 1, indicating that Der p 1-induced apoptosis is independent from TJ proteolysis.
journal_name
Mol Membr Bioljournal_title
Molecular membrane biologyauthors
Baker SF,Yin Y,Runswick SK,Stewart GA,Thompson PJ,Garrod DR,Robinson Cdoi
10.1080/0968768021000061150subject
Has Abstractpub_date
2003-01-01 00:00:00pages
71-81issue
1eissn
0968-7688issn
1464-5203pii
8WYETNJHT7TJ7XRQjournal_volume
20pub_type
杂志文章abstract::The effect of cholesterol and lanosterol on the formation of structures in the gel/subgel phase of 1,2-dipalmitoylphosphatidylcholine was investigated using freeze-fracture electron microscopy and X-ray diffraction. Mixtures with up to 25 mol% sterol were analysed after annealing for between several days and some mont...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689709048181
更新日期:1997-10-01 00:00:00
abstract::Treatment of BHK fibroblasts with V. cholerae sialidase for 20 min caused the breakdown of about 70% of total cellular ganglioside GM3 and the production of an approximately equivalent amount of lactosylceramide. On removal of the enzyme, a slow resynthesis of GM3 from lactosylceramide was observed, equivalent to abou...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689709044325
更新日期:1998-10-01 00:00:00
abstract::Dengue fever is one of the most widespread tropical diseases in the world. The disease is caused by a virus member of the Flaviviridae family, a group of enveloped positive sense single-stranded RNA viruses. Dengue virus infection is mediated by virus glycoprotein E, which binds to the cell surface. After uptake by en...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687680701633091
更新日期:2008-02-01 00:00:00
abstract::Amiodarone (AMI) is a low water-solubility drug, which is very useful in the treatment of severe cardiac disease. Its adverse effects are associated with toxicity in different tissues. Several antioxidants have been shown to reduce, and prevent AMI toxicity. The aim of this work was to develop and characterize Dimyris...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687688.2014.896956
更新日期:2014-03-01 00:00:00
abstract::The mitochondrial ADP/ATP carrier imports ADP from the cytosol into the mitochondrial matrix for its conversion to ATP by ATP synthase and exports ATP out of the mitochondrion to replenish the eukaryotic cell with chemical energy. Here the substrate specificity of the human mitochondrial ADP/ATP carrier AAC1 was deter...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687688.2012.745175
更新日期:2013-03-01 00:00:00
abstract::That some membranes restrict certain lipid species to one side of the bilayer and others to the opposite side has been known for two decades. However, how this asymmetric transbilayer distribution is generated and controlled, how many and what type of membranes are so structured, and even the reason for its existence ...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.3109/09687689409160430
更新日期:1994-10-01 00:00:00
abstract::CitS of Klebsiella pneumoniae and GltS of Escherichia coli are Na+-dependent secondary transporters from different families that are believed to share the same fold and quaternary structure. A 10 kDa protein tag (Biotin Acceptor Domain [BAD]) was fused to the N-terminus of both proteins (CitS-BAD1 and GltS-BAD1, respe...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687688.2011.581252
更新日期:2011-08-01 00:00:00
abstract::The assessment of the transverse distribution and mobility of NBD-labelled phospholipid analogues in biological membranes by selective chemical destruction of fluorescent label in the outer monolayer with dithionite has been investigated using resealed erythrocyte ghosts as a model system. The distribution of those an...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689409161028
更新日期:1994-01-01 00:00:00
abstract::Solid-state NMR combined with sample deuteration was used to probe the proximity of the low-affinity substrate D-glucose to its binding site within the Escherichia coli sugar transport protein GalP. Samples of E. coli inner membranes with amplified expression of GalP were incubated in D(2)O with D-[(13)C(6)]glucose an...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687688.2012.743193
更新日期:2013-03-01 00:00:00
abstract::The cyanelles of the glaucocystophyte alga Cyanophora paradoxa resemble endosymbiotic cyanobacteria in morphology, pigmentation and, especially, in the presence of a peptidoglycan wall situated between the inner and outer envelope membranes. However, it is now clear that cyanelles in fact are primitive plastids. Phylo...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.1080/09687860500041411
更新日期:2005-01-01 00:00:00
abstract::Nedd4 family ubiquitin ligases regulate trafficking and degradation of numerous target substrates in different cellular compartments, including at the plasma membrane, in endosomes, in the secretory pathway and in the nucleus. WWP1 is a Nedd4 family protein closely related to mouse Itch and Drosophila Su(dx), both of ...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687860600665010
更新日期:2006-05-01 00:00:00
abstract::Site-directed mutagenesis and site-directed fluorescence spectroscopy demonstrate that Cys148 interacts hydrophobically with the galactosyl moiety of substrates of the lactose permease of Escherichia coli. By taking advantage of the finding that labelling of single-Cys148 permease with the thiol-specific fluorophore 2...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689809027513
更新日期:1998-01-01 00:00:00
abstract::Membrane proteins are ubiquitous in biology and are key targets for therapeutic development. Despite this, our structural understanding has lagged behind that of their soluble counterparts. This review provides an overview of this important field, focusing in particular on the recent resurgence of electron microscopy ...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.1080/09687688.2016.1221533
更新日期:2016-03-01 00:00:00
abstract::The number of Signal Peptidase I (SPasel) molecules per E. coli cell was determined using western blot techniques. Different strains were found to contain approximately 1000 SPasel molecules per cell during exponential growth. Based on the activity of SPasel in vitro it could be estimated that this amount is sufficien...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689509072437
更新日期:1995-10-01 00:00:00
abstract::Insolubility of membrane components in non-ionic detergents such as Triton X-100 at low temperature is a widely used biochemical criterion to identify, isolate and characterize membrane domains. In this work, we monitored the detergent insolubility of the serotonin(1A) receptor in CHO cell membranes and its modulation...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687688.2012.688147
更新日期:2012-11-01 00:00:00
abstract::Radiation-based therapies aided by nanoparticles have been developed for decades, and can be primarily categorized into two main platforms. First, delivery of payload of photo-reactive drugs (photosensitizers) using the conventional nanoparticles, and second, design and development of photo-triggerable nanoparticles (...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.3109/09687688.2010.507788
更新日期:2010-10-01 00:00:00
abstract::Temperature-composition phase diagrams of binary lipid mixtures under conditions of constant pressure and excess water show that component immiscibility is the rule rather than the exception in these systems. Phase immiscibility is particularly noted in the solid phase but several cases are known where immiscibility i...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689509038493
更新日期:1995-01-01 00:00:00
abstract::Notch receptor signalling plays a central role in development and its misfunction has been linked to a number of diseases. In the cannonical Notch signalling pathway, ligand binding to Notch activates a series of proteolytic cleavages that release the Notch intracellular domain for trafficking to the nucleus, where it...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.1080/09687860500129778
更新日期:2005-07-01 00:00:00
abstract::The Amt/Mep/Rh family of integral membrane proteins comprises ammonium transporters of bacteria, archaea and eukarya, as well as the Rhesus proteins found in animals. They play a central role in the uptake of reduced nitrogen for biosynthetic purposes, in energy metabolism, or in renal excretion. Recent structural inf...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.1080/09687680701388423
更新日期:2007-09-01 00:00:00
abstract::Evidence was recently reported that the cysteine proteinase inhibitor, cystatin C, is highly expressed by cultured human retinal pigment epithelial (RPE) cells. As a step towards understanding possible functions of this protein associated with the RPE, the localization, targetting and trafficking of cystatin C were in...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687680110075101
更新日期:2001-07-01 00:00:00
abstract::2B is a 99 amino acid membrane protein encoded by enteroviruses such as polio and coxsackie viruses with two transmembrane domains. The protein is found to make membranes of infected cells permeable. Using a computational approach which positions the models and assesses stability by molecular dynamics (MD) simulations...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687680903164101
更新日期:2009-08-01 00:00:00
abstract::Palmitoylation is required for the activities of several cancer-associated proteins, making the palmitoyl acyltransferase (PAT) enzymes that catalyze these reactions potential targets for anticancer therapeutics. In this study, we sought to identify and characterize a human PAT with activity toward N-terminally myrist...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687681003616854
更新日期:2010-04-01 00:00:00
abstract::Cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-regulated C1(-) channel. Malfunction of CFTR causes cystic fibrosis (CF). CFTR belongs to an ATP-binding cassette (ABC) transporter superfamily which includes P-glycoprotein (Pgp), the molecule that is responsible for multidrug resistance in cancer c...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687689609160572
更新日期:1996-01-01 00:00:00
abstract::This review describes the numerous and innovative methods used to study the structure and function of viral fusion peptides. The systems studied include both intact fusion proteins and synthetic peptides interacting with model membranes. The strategies and methods include dissecting the fusion process into intermediat...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.3109/09687689709048170
更新日期:1997-07-01 00:00:00
abstract::The molecular events and the protein components that are involved in signalling by the T cell receptor (TCR) for antigen have been extensively studied. Activation of signalling cascades following TCR stimulation depends on the phosphorylation of the receptor by the tyrosine kinase Lck, which localizes to the cytoplasm...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.1080/09687860500453673
更新日期:2006-01-01 00:00:00
abstract::Numerous cell surface receptors propagate activation signals to the interior of the cell via tyrosine phosphorylation of transmembrane proteins. This leads to the phosphotyrosine (PiY)-mediated recruitment of cytoplasmic signalling protein complexes which catalyze crucial biochemical signalling reactions. Here we desc...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687680802637660
更新日期:2009-04-01 00:00:00
abstract::Membrane microdomains enriched in cholesterol, sphingolipids (rafts), and specific proteins are involved in important physiological functions. However their structure, size and stability are still controversial. Given that detergent-resistant membranes (DRMs) are in the liquid-ordered state and are rich in raft-like c...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.3109/09687688.2014.949319
更新日期:2014-09-01 00:00:00
abstract::Previous work from our laboratory has established bovine hippocampal membranes as a convenient natural source for studying neuronal receptors such as the G-protein coupled serotonin1A receptor. In this paper, we have explored the organization and dynamics of bovine hippocampal membranes using environment-sensitive and...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687860600803223
更新日期:2006-09-01 00:00:00
abstract::Caveolae are approximately 50-100 nm membrane micro-invaginations associated with the plasma membrane of a wide variety of cells. Although they were first identified in transmission electron micrographs approximately 40 years ago, their exact function(s) has remained controversial. Two well-established functions inclu...
journal_title:Molecular membrane biology
pub_type: 杂志文章,评审
doi:10.3109/09687689509038506
更新日期:1995-01-01 00:00:00
abstract::A structural characterization of a synthetic peptide corresponding to the fourth transmembrane domain (M4-TMD) of the gamma-subunit of the nicotinic acetylcholine receptor from Torpedo californica has been undertaken. Solid-state NMR and CD spectroscopy studies indicate that upon reconstitution into lipid vesicles or ...
journal_title:Molecular membrane biology
pub_type: 杂志文章
doi:10.1080/09687860500370653
更新日期:2005-11-01 00:00:00