Abstract:
:The goal of the study was to investigate changes in expression of selected growth factors tentatively involved in regeneration of haematopoietic tissues (bone marrow and spleen) following cyclophosphamide (CY) damage in the mouse. The bone marrow (BM) and spleen were examined separately, since the regenerating pattern for haematopoietic progenitor cells (HPC) markedly differs in these two haematopoietically active organs after CY. Cytokines assumed to have a stimulatory effect on HPC - stem cell factor (SCF), fetal liver tyrosine kinase 3-ligand (flt3-ligand), thrombopoietin (TPO), stromal cell-derived factor 1 (SDF-1), oncostatin M (OSM) -, a suppressive effect on HPC proliferation - macrophage inflammatory protein-1alpha (MIP-1alpha), transforming growth factor-beta1 (TGFbeta1), tumour necrosis factor-alpha (TNFalpha) - and to be involved in migration of HPC (SCF, flt3-ligand, MIP1alpha, SDF-1) were examined at the level of mRNA expression by means of real-time RT-PCR. The expression of a particular cytokine appears to be similar in both BM and spleen of untreated mice. CY administration changed the expression pattern of the studied genes in BM and spleen. In BM, the levels of mRNAs for SCF and SDF-1 were increased and that for TGFbeta1 decreased at time intervals at which HPC are known to proliferate intensively during BM regeneration. In contrast, stimulated proliferation of HPC in spleen was accompanied by increased expression of flt3-ligand and oncostatin M. Upon mobilization of HPC from BM into blood after CY, the expression of SCF, TPO, SDF-1 and TGFbeta1 tends to decrease in BM. Accumulation of HPC in spleen is accompanied by increased mRNA for flt3-ligand and OSM. Our findings demonstrate that different cytokines may be involved in the proliferation and mobilization/homing of HPC during recovery after CY damage in BM and spleen.
journal_name
Acta Haematoljournal_title
Acta haematologicaauthors
Psenák O,Sefc L,Sýkora V,Chang KT,Necas Edoi
10.1159/000068490subject
Has Abstractpub_date
2003-01-01 00:00:00pages
68-75issue
2eissn
0001-5792issn
1421-9662pii
68490journal_volume
109pub_type
杂志文章abstract::We have studied the amount and intracellular distribution of erythroblastic alkaline and acid phosphatase, nonspecific esterase, and N-acetyl-beta-glucosaminidase in 50 patients with acquired dyserythropoiesis. 19 morphologically normal bone marrow smears served as controls. Alkaline phosphatase was found in all contr...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000206406
更新日期:1984-01-01 00:00:00
abstract::To clarify the role of thrombopoietin (c-Mpl ligand, TPO) in 'hypersplenic' thrombocytopenia, we used an enzyme-linked immunosorbent assay to examine changes in serum TPO levels accompanied with splenectomy in 6 patients with liver cirrhosis, 4 patients with gastric cancer, and 2 patients with lymphoid malignancies. W...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000040888
更新日期:1998-12-01 00:00:00
abstract::Fetal hemoglobin (HbF) concentrations were measured by a radial immunodiffusion assay in 233 patients with various malignancies. In 96 of these, alpha-fetoprotein (AFP) was also measured by radioimmunoassay. The concentration of HbF exceeded 2 SDs above the normal mean in 39 of 233 patients, most notably in patients w...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000207839
更新日期:1977-01-01 00:00:00
abstract::A 13-year-old Saudi girl presented with severe clinical features of systemic lupus erythematosus of 3 months' duration. In addition to laboratory evidence of the disease, the patient was found to have pancytopenia and myelofibrosis. While pancytopenia was considerably improved by steroids, myelofibrosis was not revers...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000205641
更新日期:1988-01-01 00:00:00
abstract::Avascular necrosis (AVN) of the hip is a common cause of morbidity in sickle cell disease (SCD). Its prevalence increases with age and predisposing factors include coexistent alpha-thalassemia trait, frequent vaso-occlusive crisis and a high hematocrit (Hct). SCD is relatively mild among Kuwaiti patients because of th...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000046529
更新日期:2001-01-01 00:00:00
abstract::Fetal liver transplantation was attempted in 7 patients with aplastic anemia. 4 of these patients showed a partial response as evidenced by decrease in blood transfusion requirements and increase in the peripheral blood counts and hematopoietic cells in the bone marrow. Bone marrow culture studies revealed evidence of...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000207558
更新日期:1979-01-01 00:00:00
abstract::A 67-year-old woman with agnogenic myeloid metaplasia, who underwent splenectomy 1 year after the diagnosis, experienced spontaneous hematologic remission 6 years after splenectomy. Ten months before her anemia improved, peripheral leukocytes no longer showed a shift to the left and peripheral erythroblasts had disapp...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000205163
更新日期:1990-01-01 00:00:00
abstract::We examined the in vivo effect of granulocyte colony-stimulating factor (G-CSF) on the surface expression of putative counterligands for endothelial selectins on neutrophils in healthy volunteers. G-CSF (50 microg/m2/day) was administered subcutaneously to 5 healthy volunteers for 4 days. The expression of surface ant...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000040901
更新日期:1998-01-01 00:00:00
abstract::It has been proposed that iron overload may adversely affect liver disease outcome. The recent identification of 2 mutations in the HFE gene related to hereditary haemochromatosis (Cys282Tyr and His63Asp) provided an opportunity to test whether they are associated with hepatic iron accumulation and the activity and se...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000040991
更新日期:2000-01-01 00:00:00
abstract::The last few years have seen an enormous increase in our knowledge on the haematopoietic growth factor erythropoietin (Epo), firstly with its purification and determination of its primary amino acid sequence, and more recently with the isolation of the Epo gene and its expression in mammalian cell lines. This review a...
journal_title:Acta haematologica
pub_type: 杂志文章,评审
doi:10.1159/000204826
更新日期:1991-01-01 00:00:00
abstract::Neurologic complications of allogeneic hematopoietic cell transplantation (allo-HCT) include infections, cerebrovascular events, therapy-induced neurotoxicity, recurrent malignancies, and neurologic manifestations of graft-versus-host disease (GVHD). Anti-glutamic acid decarboxylase (GAD) antibody-associated cerebella...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000494423
更新日期:2019-01-01 00:00:00
abstract::Diffuse large B cell lymphoma, T-cell-rich/histiocyte-rich variant (DLBL-TH), is characterized by the presence of neoplastic B cells set in a background containing numerous non-neoplastic T lymphocytes and histiocytes. We report here the case of a patient with DLBL-TH who developed overt pure red cell aplasia (PRCA) f...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000084450
更新日期:2005-01-01 00:00:00
abstract::Thrombopoietin levels in thrombocytopenic mice assayed by 75Se-se-lenomethionine incorporation into blood platelets reached a maximum 12 h after the induction of an acute, immune thrombocytopenia; that was more than twice the value in control mice. The implications of this finding are discussed with reference to the k...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000208096
更新日期:1975-01-01 00:00:00
abstract::Micromegakaryocytes (MMK) were defined morphologically by the cell area, nucleus form and cytoplasmic structure. Bone marrow smears of 7,156 patients were retrospectively analyzed. MMK were found most frequently and abundantly in acute non-lymphatic leukaemia, chronic myeloid leukaemia and pre-leukaemia. The presence ...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000207213
更新日期:1980-01-01 00:00:00
abstract::The 8p11 myeloproliferative syndrome (EMS) is an aggressive neoplasm associated with chromosomal translocations involving the fibroblast growth factor receptor 1 (FGFR1) tyrosine kinase gene on chromosome 8p11-12. A new case of a 9-year-old boy with leukocytosis, eosinophilia, and general lymphadenopathy is reported i...
journal_title:Acta haematologica
pub_type: 杂志文章,评审
doi:10.1159/000481392
更新日期:2018-01-01 00:00:00
abstract::We present a case with the clinical and pathological impression of Ph1-positive chronic myelogenous leukemia in extramedullary blast crisis involving lymph nodes as demonstrated by morphological and cytogenetic studies. The hyperploid cell lines that were present in the lymph node were not present in the bone marrow. ...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000206166
更新日期:1985-01-01 00:00:00
abstract::Dihydrofolate reductase (FH2-R) was studied cytochemically in the bone marrow erythroblasts of 20 normal controls and 46 patients with myelodysplastic syndromes (MDSs) classified according to FAB, prior to therapy. The reaction product was quantified for the same samples with a Vickers M86 microdensitometer. The enzym...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000205807
更新日期:1988-01-01 00:00:00
abstract::While sickle cell disease (SCD) is generally mild in most Kuwaitis, because of their elevated fetal Hb levels, avascular necrosis of the femoral head (AVNFH) appears to be a common complication. It was recently documented in 26.7% of Kuwaiti children with SCD. There have, however, been no previous studies of adult pat...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000072406
更新日期:2003-01-01 00:00:00
abstract::Tumor cell (TC) contamination of stem cell products can contribute to relapse after high dose chemotherapy and stem cell rescue. A new purging technology using replication-deficient recombinant adenovirus (Adv) containing the p53 tumor suppressor gene (Adv-p53) has been suggested to reduce tumor contamination of autol...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000040931
更新日期:1999-01-01 00:00:00
abstract::We present the case of a woman undergoing treatment with acenocoumarol for deep vein thrombosis, who maintained an international normalized ratio (INR) of between 2.5 and 4 for 2 months. Seven days after the introduction of amoxycillin (500 mg/8 h) for a probable respiratory infection, the patient developed spontaneou...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000204457
更新日期:1993-01-01 00:00:00
abstract::Iron overload due to regular transfusions of packed red cells can cause multiple organ damage. Iron chelation therapy (ICT) is important in patients with aplastic anemia (AA) who require blood transfusions as supportive management. With the introduction of the oral iron chelator deferasirox, ICT has become more widely...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000342772
更新日期:2013-01-01 00:00:00
abstract::Chromosomal aberrations in a case of atypic myeloproliferative syndrome are reported. The analysis was carried out on short-term cultures of sternal bone marrow with the ASG and fluorescence method. 90% of the metaphases studied showed pseudoliploidy: one of the chromosomes 12 was missing and one submedian marker chro...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000208052
更新日期:1975-01-01 00:00:00
abstract::Alpha-thalassemia is very common in the Kuwaiti population, but its influence on anemia of pregnancy has not been previously investigated. We have screened a group of 59 anemic (Hb < 11 g/dl) pregnant women for the alpha-thal-2 (-alpha-3.7 kb) deletion which is the commonest alpha-thal allele in this community, using ...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000039757
更新日期:2000-01-01 00:00:00
abstract::A family with hereditary ovalocytosis (HO) is described. The probands, 2 brothers, had splenic rupture after modest trauma as preenting symptoms. 7 members of the family had HO. The sister of the pobands had a moderately enlarged spleen. The other members proved normal on routine clinical examination. ...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000207420
更新日期:1980-01-01 00:00:00
abstract::In order to study the abnormal differentiation and proliferation of precursor T cells in homosexual men, a study was made of T-cell colony formation in 15 healthy homosexual men without anti-human immunodeficiency virus (HIV) antibody. The colony forming method used was the one-step monolayer technique using phytohema...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000205374
更新日期:1989-01-01 00:00:00
abstract::An improved plasma clot culture method for CFU megakaryocytes (MK) has been developed with a higher plating efficiency and easier identification and enumeration of MK colonies by an indirect immunoperoxidase staining using a monoclonal antibody specific to the platelet glycoprotein IIb/IIIa complex. This technique has...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000205837
更新日期:1987-01-01 00:00:00
abstract::The most common single genetic disorder and a major public health issue in Greece and other Mediterranean countries is beta-thalassemia. Current therapeutic approaches for homozygous beta-thalassemia entail blood transfusions and iron chelation therapy with deferoxamine or deferiprone for preventing tissue hemosideros...
journal_title:Acta haematologica
pub_type: 临床试验,杂志文章
doi:10.1159/000077551
更新日期:2004-01-01 00:00:00
abstract::The objective of this study was to assess the pharmacokinetics of rhG-CSF after a single intraperitoneal injection 2 h post-TBI in B6D2F1 lethally irradiated mice and to analyze the effect of rhG-CSF on the endogenous response of interleukin-3 (IL-3), interleukin-6 (IL-6) and granulocyte-macrophage colony-stimulating ...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000203604
更新日期:1997-01-01 00:00:00
abstract::A 69-year-old woman presented with chronic lymphocytic leukemia (CLL) diagnosed after bone marrow recovery following intensive chemotherapy for acute lymphoblastic leukemia. The retrospective diagnosis of initial blastic transformation of CLL was made. The patient eventually died, 5 months after initial blastic phase,...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000205751
更新日期:1988-01-01 00:00:00
abstract:BACKGROUND:The incidence of venous thromboembolism (VTE) in haematological malignancies varies according to the type and grade of the disease and clinical variables, and there is a need to develop a tool to predict the occurrence of VTE in cancer patients at diagnosis to tailor prophylactic anticoagulation use during t...
journal_title:Acta haematologica
pub_type: 杂志文章
doi:10.1159/000496914
更新日期:2019-01-01 00:00:00